Tissue Culture Method of Hemerocallis grandiflora Christmas Red
A tissue culture, Hemerocallis Hemerocallis technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of large market demand, small number of introductions, slow ramets, etc., to improve the survival rate and increase the output. , the effect of increasing the speed of reproduction
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Embodiment 1
[0028] The tissue culture method of Hemerocallis grandiflora Christmas red, the method comprises the following steps:
[0029] (1) Obtaining sterile materials
[0030] Pick the flower buds when Hemerocallis 'Christmas Red' blooms, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 20s, soak them in 1wt‰ mercury chloride solution for 10mins, and rinse them with sterile water for 5 times Blot the surface moisture with sterile filter paper, get the bud base and cut it into 1cm length, and inoculate it on the bud induction medium. The composition of the bud induction medium is MS+KT0.1mg / L+NAA1.0mg / L;
[0031] (2) Differentiation and proliferation of buds
[0032] 6 weeks after the flower buds were inoculated on the flower bud induction medium, the flower buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. Continue to culture for 1 month, and then cut off the callus...
Embodiment 2
[0041] The tissue culture method of Hemerocallis grandiflora Christmas red, the method comprises the following steps:
[0042] (1) Obtaining sterile materials
[0043] Pick the flower buds when Hemerocallis 'Christmas Red' blooms, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 40s, soak them in 1wt‰ mercury chloride solution for 20mins, and rinse them with sterile water for 6 times Blot the surface moisture with sterile filter paper, get the flower bud base and cut it into 1 cm length, and inoculate it on the flower bud induction medium. The composition of the flower bud induction medium is MS+KT0.5mg / L+NAA5.0mg / L;
[0044] (2) Differentiation and proliferation of buds
[0045] 6 weeks after the flower buds were inoculated on the flower bud induction medium, the flower buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. Continue to culture for 1 month, and t...
Embodiment 3
[0054] The tissue culture method of Hemerocallis grandiflora Christmas red, the method comprises the following steps:
[0055] (1) Obtaining sterile materials
[0056] Pick the flower buds when Hemerocallis 'Christmas Red' blooms, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 30s, soak them in 1wt‰ mercury chloride solution for 15mins, and rinse them with sterile water for 5 times Blot the surface moisture with sterile filter paper, get the flower bud base and cut it into 1 cm length, and inoculate it on the flower bud induction medium. The composition of the flower bud induction medium is MS+KT0.3mg / L+NAA3.0mg / L;
[0057] (2) Differentiation and proliferation of buds
[0058] 6 weeks after the flower buds were inoculated on the flower bud induction medium, the flower buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. Continue to culture for 1 month, and t...
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