Tissue Culture Method of Hemerocallis grandiflora Christmas Red

A tissue culture, Hemerocallis Hemerocallis technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of large market demand, small number of introductions, slow ramets, etc., to improve the survival rate and increase the output. , the effect of increasing the speed of reproduction

Active Publication Date: 2011-12-07
上海上房园艺有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the small number of imported varieties introduced from abroad, the ramets are slow, the market demand is large, and the supply of seedlings is limited
At present, there are no domestic reports on the tissue culture of Hemerocallis grandiflora 'Christmas Red'

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The tissue culture method of Hemerocallis grandiflora Christmas red, the method comprises the following steps:

[0029] (1) Obtaining sterile materials

[0030] Pick the flower buds when Hemerocallis 'Christmas Red' blooms, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 20s, soak them in 1wt‰ mercury chloride solution for 10mins, and rinse them with sterile water for 5 times Blot the surface moisture with sterile filter paper, get the bud base and cut it into 1cm length, and inoculate it on the bud induction medium. The composition of the bud induction medium is MS+KT0.1mg / L+NAA1.0mg / L;

[0031] (2) Differentiation and proliferation of buds

[0032] 6 weeks after the flower buds were inoculated on the flower bud induction medium, the flower buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. Continue to culture for 1 month, and then cut off the callus...

Embodiment 2

[0041] The tissue culture method of Hemerocallis grandiflora Christmas red, the method comprises the following steps:

[0042] (1) Obtaining sterile materials

[0043] Pick the flower buds when Hemerocallis 'Christmas Red' blooms, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 40s, soak them in 1wt‰ mercury chloride solution for 20mins, and rinse them with sterile water for 6 times Blot the surface moisture with sterile filter paper, get the flower bud base and cut it into 1 cm length, and inoculate it on the flower bud induction medium. The composition of the flower bud induction medium is MS+KT0.5mg / L+NAA5.0mg / L;

[0044] (2) Differentiation and proliferation of buds

[0045] 6 weeks after the flower buds were inoculated on the flower bud induction medium, the flower buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. Continue to culture for 1 month, and t...

Embodiment 3

[0054] The tissue culture method of Hemerocallis grandiflora Christmas red, the method comprises the following steps:

[0055] (1) Obtaining sterile materials

[0056] Pick the flower buds when Hemerocallis 'Christmas Red' blooms, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 30s, soak them in 1wt‰ mercury chloride solution for 15mins, and rinse them with sterile water for 5 times Blot the surface moisture with sterile filter paper, get the flower bud base and cut it into 1 cm length, and inoculate it on the flower bud induction medium. The composition of the flower bud induction medium is MS+KT0.3mg / L+NAA3.0mg / L;

[0057] (2) Differentiation and proliferation of buds

[0058] 6 weeks after the flower buds were inoculated on the flower bud induction medium, the flower buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. Continue to culture for 1 month, and t...

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PUM

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Abstract

The invention relates to a tissue culturing method of hemerocallis middendorfii poinsettia. The method comprises steps that: when hemerocallis poinsettia blooms, flower buds are selected, or newly grown buds are selected in early spring; sterile materials are obtained; the buds are differentiated and multiplied; adventitious bud strong seedlings are cultured; rooting culturing, acclimatization and transplantation are carried out upon the buds, such that hemerocallis poinsettia plants are obtained. Compared to prior arts, according to the invention, through the tissue culturing method, multiplication speed and nursery stock regularity can be substantially improved, original female parent properties can be maintained, survival rate can be improved, and the yield can be improved. With the method, the transplantation survival rate of hemerocallis middendorfii poinsettia reaches 95%.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of Hemerocallis grandiflorum Christmas red. Background technique [0002] Hemerocallis grandiflora 'Christmas red' is a perennial herb of the genus Hemerocallis in the family Liliaceae, with tender green leaves and beautiful clusters of green leaves; it blooms in early summer, with large, funnel-shaped flowers, about 10cm in diameter, red, yellow in the center of the flower, brightly colored, It is easy to cultivate, has few pests and diseases, and has strong adaptability to the environment. Hemerocallis is widely used in gardens because of its strong ornamental value and adaptability to the environment. It can be planted as a ground cover in large areas, or it can be planted in clusters or arranged in flower borders. It is also an excellent material for beautifying small gardens. At present, the common varieties of daylilies in China are the yellow golden...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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