31results about How to "Maintain maternal traits" patented technology

The invention relates to a tissue culturing method of hemerocallis middendorfii poinsettia. The method comprises steps that: when hemerocallis poinsettia blooms, flower buds are selected, or newly grown buds are selected in early spring; sterile materials are obtained; the buds are differentiated and multiplied; adventitious bud strong seedlings are cultured; rooting culturing, acclimatization and transplantation are carried out upon the buds, such that hemerocallis poinsettia plants are obtained. Compared to prior arts, according to the invention, through the tissue culturing method, multiplication speed and nursery stock regularity can be substantially improved, original female parent properties can be maintained, survival rate can be improved, and the yield can be improved. With the method, the transplantation survival rate of hemerocallis middendorfii poinsettia reaches 95%.

The invention relates to a breeding method of a new variety of banana, which comprises the five steps of sterile material acquisition, bud differentiation and proliferation, adventitious bud strong stock cultivation, rooting cultivation, and seedling adaptation and transplantation to obtain banana sprouts. Compared with the prior art, through a tissue culture technique, the breeding speed and theneatness of nursery stock can be greatly increased, the original female parent properties can be better maintained, the survival rate can be improved, thus, the output is increased, and the transplanting survival rate can reach 95%.

The invention provides a method for cutting crape myrtle seedlings on a seedbed and belongs to the technical field of plant cutting methods. The method is characterized by comprising the following steps of 1 setting up a greenhouse; 2 arranging the seedbed; 3 preparing cuttings; 4 performing cutting; 5 performing field management. By means of the method for cutting the crape myrtle seedlings on the seedbed, the variety number can be rapidly increased, female parent characters can be kept, the root quality and the growth situation of aboveground parts are good, the same-area cutting amount is large, and the cutting survival rate reaches to 93%.

The invention relates to a tissue culture method of nandina domestica firepower, and the method is based on newly germinated buds of nandina domestica firepower, and comprises the following steps: obtaining a sterile material, performing differentiation and proliferation of buds, performing strong seedling culture and rooting culture of adventitious buds, performing seedling hardening and seedling transplanting so as to finally obtain a nandina domestica firepower plant with good characters. Compared with the prior art, the invention has a transplanting survival rate of up to 95%, greatly increases the reproductive speed and the seedling uniformity, and maintains original female parent characters better.

The invention relates to a flaxbush tissue culture method. The method comprises the following steps: (1) preparing a culture medium comprising components and content of the culture medium in each stage during tissue culture, 1) a bud induction culture medium: MS+6-BA(1.0-5.0)mg/L+NAA(0.1-0.5)mg/L; 2) an adventitious bud proliferation culture medium: MS+6-BA(1.0-3.0)mg/L+NAA(0.1-0.3)mg/L; 3) a strong seedling culture medium: MS+6-BA(0.5-2.0)mg/L+NAA(0.1-0.2)mg/L; 4) a rooting culture medium: MS+NAA(0.05-0.2)mg/L, wherein the ph values of the culture mediums under various conditions is 5.8; adding 30g/L of cane sugar, 6g/L of agar, the culture temperature is 25+/-1 DEG C, and the illumination is about 80 micromoles. Compared with the prior art, the flaxbush tissue culture method has the advantages that the breeding speed and the seedling uniformity can be greatly improved, and the original female parent characters are well kept.

The invention relates to a method for in vitro culture of hemerocallis middendorfii Trautv. et Mey. tissue. The method comprises the following steps: picking a small bud on a flower stalk when hemerocallis middendorfii Trautv. et Mey. is in the blooming season, performing sterile processing on the small bud, and sequentially performing bud differentiation and proliferation, strong adventitious bud seedling culture, rooting culture, seedling exercising and transplanting to obtain the hemerocallis middendorfii Trautv. et Mey. Compared with the prior art, the year propagation coefficient of the hemerocallis middendorfii Trautv. et Mey. can be increased to 1: 100,000 or even higher, and the cost can be reduced to 0.5-0.8 yuan/strain, so that the reproduction rate and the uniformity of seedlings are greatly improved, and original female parent characters are better maintained.

The invention relates to a rapid breeding method based on tissue culture for ligularia kaempferi. The method comprises the following steps: (1) preparing a bud inducing culture medium, an adventitiousbud proliferation culture medium, a seedling strengthening culture medium, and a rooting culture medium; and (2) performing tissue culture on the ligularia kaempferi. According to the method providedby the invention, through a tissue culture technology the annual propagation coefficient is improved to 1:50000 and the cost is reduced by 0.8-1 yuan/plant compared with those of the prior art; and because the floral leaves of the leaves are a yellow-green chimera, the difficulty of the tissue culture is larger, through adjustment of the ratio of auxin to cytokinin, the characteristics of the leaves are obvious, the propagation speed and the seedling uniformity are greatly improved, and the original female parent characters are better maintained.

The invention relates to a tissue culture method of dianella ensifolia. The tissue culture method comprises the steps: respectively using a budlet induction medium for carrying out bud induction culture, a proliferation medium for carrying out proliferation culture, a strong seedling medium for carrying out strong seedling culture and a rooting medium for rooting culture, then carrying out domestication and transplanting so as to obtain dianella ensifolia plants. Compared with the prior art, the tissue culture method has the advantages that the reproduction speed and the evenness degree of seedlings are greatly increased through a tissue culture technology, the original female parent characters are maintained well, and the survival rate of transplanting can reach 95%.

The invention belongs to the technical field of asexual reproduction of wild ganoderma lucidum karst, and specifically relates to a cultivation method for wild ganoderma lucidum karst. The cultivationmethod includes ganoderma lucidum karst obtaining, mother strain preparation, original strain preparation, production strain cultivation and ganoderma lucidum karst fruit body cultivation; the raw materials of a mother strain culture medium include potatoes, rice milk, sugar, agar and water; the raw materials of an original strain culture medium include wood chips, platycodon grandiflorum powder,wheat bran, wheat, corns, gypsum, the sugar and calcium carbonate, with the balance being water; and the raw materials of a production strain culture medium include the wood chips, sweet potato flour, the wheat, the corns, soybean meal and the sugar, with the balance being water. The cultivation method utilizes the wild ganoderma lucidum karst as a material to reproduce strains indoors without limiting by seasons, so that quantity demands on production can be met, maternal characters can also be maintained, high-quality ganoderma lucidum karst strains suitable for local development can be effectively cultivated, and large-scale production of ganoderma lucidum karst industries can be realized; and the cultivation method has good economic, social and ecological benefits.

The invention discloses a method for the tissue culture of long-ear kniphofia. The method comprises the steps of picking up long-ear kniphofia budlet, washing, culturing by a bud induction medium, putting cultured calluses with buds into a multiplication medium for differentiation multiplication, carrying out adventitious bud cultivation and rooting cultivation, and obtaining the long-ear kniphofia plant through the seedling hardening technology and the transplanting technology. Compared with the prior art, according to the tissue culture technology, the maturing rate can be improved, and the different nutrition facts can be provided at the different cultivation stages by the selected bud induction culture medium, multiplication culture medium, seedling boosting culture medium and rooting culture medium, so that the original female characters can be kept to the maximum, and the transplanting survival rate in the steps of seedling hardening and transplanting can reach 90%.

The invention belongs to the field of tissue culture of medicinal plants, and discloses an amomum tsao-ko tissue culture and seedling raising method. The amomum tsao-ko tissue culture and seedling raising method comprises the following steps of: selecting an explant: selecting the young stem segment at the base of an amomum tsao-ko stem; disinfecting the explant; performing primary induction culture; performing secondary enrichment culture; and performing rooting culture. According to the amomum tsao-ko tissue culture and seedling raising method disclosed by the invention, the adopted explantis the young stem segment at the base of the stem; compared with young shoots near the soil surface adopted at the beginning of an experiment, the contamination rate is decreased to 12% at the young stem segment from 95% at the young shoots near the soil surface; but, the inductivity of the young stem segment at the base of the stem is 80%, and slightly lower than the inductivity, namely, 84%, atthe young shoots near the soil surface.

The invention relates to a tissue culture method of baptisia australis. The tissue culture method comprises the steps of sterile treatment, differentiation and proliferation, strong seedling of adventitious buds, rooting culture, acclimatization and transplanting, and the like. The step-by-step tissue culture of the baptisia australis is carried out by using a bud induction medium, a proliferation medium, a strong seedling medium and a rooting medium. Compared with the prior art, the tissue culture method has the advantages that the baptisia australis is subjected to step-by-step tissue culture by selecting the appropriate mediums, so that the reproduction speed and the evenness degree of nursery stocks are increased, the original female parent characters are maintained well, and in addition, the survival rate of transplanting can reach 85%.

The invention belongs to the technical field of forestry reproduction and discloses a reproduction and transplantation regulator for reddening leaves of acer palmatum and a reproduction and planting method of the acer palmatum. The reproduction and transplantation regulator is prepared from components as follows: indolebutyric acid, magnesium carbonate, ferric phosphate, zinc sulfate, aluminum sulfate, ammonium nitrate and potassium stearate. All that is required for large-scale planting is to rapidly dip cuttings in the regulator, and the reproduction and transplantation regulator is particularly suitable for being used in gardens, municipal engineering, roads and landscape industries. The reproduction and transplantation regulator can make acer palmatum have uniform colors, redder leaves and higher ornamental value, leaf yellowing, leaf spots and powdery mildew are prevented and treated, the cutting survival rate is high, and the cutting number is large in the same area.