31results about How to "Maintain maternal traits" patented technology

The invention relates to a cultivating method of Hosta plantaginea Gold Standard. The method comprises the following steps of: first carrying out sterile treatment on Hosta plantaginea Gold Standard buds excavated in the early spring; and then inoculating the buds on a bud inducing culture medium to differentiate the buds; culturing the differentiated buds for 1 month; and then cutting off a budding callus and putting the budding callus into a proliferation medium to be proliferated so as to obtain cluster buds; after dividing the cluster buds into shoots or single buds, putting the shoots orthe single buds into a seedling culture medium to culture the seedling with adventitious buds; obtaining 2-3 cm small plants after 20 days; transferring the small plants into a rooting culture mediumto be induced for rooting; selecting strongly growing sterile seedlings with developed roots; carrying out seedling adaptation indoors in an open bottle for three days; washing off agars at the rootsof the seedlings after taking out the seedlings; transplanting the seedlings outdoors after naturalizing the seedlings in a greenhouse for 40 days; and carrying out fertilizer and water management onthe seedlings. Compared with the prior art, the cultivating method of the Hosta plantaginea Gold Standard provided by the invention has the following advantages that: the reproductive speed and the evenness of the nursery-grown plants can be largely increased; the original maternal character is preferably kept; and the survival rate can be increased so that the yield is increased and the transplanting survival rate can reach 95%.

The invention discloses a quick cultivation method of Rosa multiflora thunb.var.cathayensis. a rooting powder solution is NAA with the concentration of 300 mg.L<-1>, red-yellow soil serves as a cutting medium, one-year-old stems with leaves serve as cuttings, treatment time is four hours, the cutting substrate which is 8-10 cm thick covers a seedbed, the processed cutting scions are inserted by being punched by a wood stick according to the plant spacing of 15 cm*15 cm, the insertion depth is 1 / 2-2 / 3 of the length of the scions, the soil is sufficiently watered immediately, and the temperature is 20-25 DEG C. The method is high in cutting survival rate and rooting rate and good in quality, and lays a foundation for large-scale seedling raising of Rosa multiflora thunb.var.cathayensis.

The invention relates to a method for culturing tissues of Buxus sempervives. The method comprises the following steps of: picking budlets on the upper part of the Buxus sempervives, and sequentially performing sterile treatment, differentiating and proliferating buds, performing hardening-off culture of adventitious buds, performing rooting culture, exercising seedlings and transplanting to obtain seedlings of the Buxus sempervives. Compared with the prior art, the method has the advantages that: by a tissue culture technology, the reproductive speed and the uniformity of nursery stocks can be greatly improved, the original maternal character can be better maintained, and the survival rate can be improved, so that the yield is improved; and the transplantation survival rate can reach 95 percent.

The invention relates to a tissue culture method of president clematis. Tender shoots of 'president' clematis are selected as a raw material; and a clematis 'president' plant is acquired through steps of an aseptic processing, a differentiation and propagation of tender shoots, a strong seedling culture of adventitious buds, a rooting culture of a root, a plant hardening seedling and transplanting, etc. Compared with a prior art, the invention employs tissue culture to increase a breeding speed of nursery stock and regularity of the seedlings, to better maintain original female parent properties, and to realize factory seedling growing, so as to guarantee market supply demand.

The invention relates to a method for cultivating gold-leaf sedge. The method comprises the following steps of: picking a budlet of gold-leaf sedge; and performing sterile pretreatment, differentiation and multiplication of the budlet, adventitious bud acclimatizing culturing, rooting culturing, seedling adaptation and transplanting to obtain a gold-leaf sedge plant. Compared with the prior art, the method has the advantages that: by adopting a tissue culturing technology, the reproduction speed and the regularity of nursery stock can be greatly increased, the original maternal character is better kept, and the survival rate can be increased, so that the yield is increased and the transplanting survival rate is up to 95 percent.

The invention relates to a method for culturing red-petiole taros. The method comprises the following steps: (1) obtaining sterile material; (2) differentiating and proliferating buds; (3) culturing adventitious bud strong seedlings; (4) striking roots and culturing; and (5) acclimatizing and transplanting. Compared with the prior art, the method has the advantages that the propagation speed and the neatness of seedlings can be greatly increased through a tissue culture technology, the trait of a female patient is better retained, and the survival rate can be increased, therefore, the yield is increased, and the transplanting survival rate can be up to 95%.

The invention belongs to the technical field of asexual propagation of wild ganoderma, and in particular relates to a cultivation method of wild ganoderma. A cultivation method of wild Ganoderma lucidum, comprising the acquisition of Ganoderma lucidum entities, preparation of mother seeds, preparation of original seeds, cultivation of production species and cultivation of fruiting bodies of Ganoderma lucidum, wherein the raw materials of the mother seed culture medium include potatoes, rice milk, sugar, agar, and water; the original seeds The medium raw materials include sawdust, bellflower powder, wheat bran, wheat, corn, gypsum, sugar, calcium carbonate, and the balance is water; the production medium raw materials include sawdust, sweet potato powder, wheat, corn, soybean flour, sugar, and the balance It is water; the present invention uses wild ganoderma as a material to propagate strains indoors without seasonal restrictions, which can not only meet the quantity demand in production, but also maintain the traits of the female parent, and effectively select and breed high-quality ganoderma suitable for local development species, the large-scale production of Ganoderma lucidum industry has been realized, and it has good economic, social and ecological benefits.

The invention relates to a tissue culture method for heuchera micrantha 'Palace Purple'. The method comprises the following steps of: obtaining sterile materials; differentiating and proliferating buds; culturing strong seedlings of adventitious buds; rooting; and hardening off seedlings and transplanting the seedlings and the like. Compared with the prior art, the method greatly improves the reproductive speed of the heuchera micrantha 'Palace Purple' and the trimming of the seedlings, and improves the stability of shape and properties of the heuchera micrantha 'Palace Purple', so that the industrial mass production of the seedlings can be realized.

The invention relates to a tissue culture method of nandina domestica firepower, and the method is based on newly germinated buds of nandina domestica firepower, and comprises the following steps: obtaining a sterile material, performing differentiation and proliferation of buds, performing strong seedling culture and rooting culture of adventitious buds, performing seedling hardening and seedling transplanting so as to finally obtain a nandina domestica firepower plant with good characters. Compared with the prior art, the invention has a transplanting survival rate of up to 95%, greatly increases the reproductive speed and the seedling uniformity, and maintains original female parent characters better.