31results about How to "Maintain maternal traits" patented technology

The invention relates to a tissue culturing method of hemerocallis middendorfii poinsettia. The method comprises steps that: when hemerocallis poinsettia blooms, flower buds are selected, or newly grown buds are selected in early spring; sterile materials are obtained; the buds are differentiated and multiplied; adventitious bud strong seedlings are cultured; rooting culturing, acclimatization and transplantation are carried out upon the buds, such that hemerocallis poinsettia plants are obtained. Compared to prior arts, according to the invention, through the tissue culturing method, multiplication speed and nursery stock regularity can be substantially improved, original female parent properties can be maintained, survival rate can be improved, and the yield can be improved. With the method, the transplantation survival rate of hemerocallis middendorfii poinsettia reaches 95%.

The invention relates to a breeding method of a new variety of banana, which comprises the five steps of sterile material acquisition, bud differentiation and proliferation, adventitious bud strong stock cultivation, rooting cultivation, and seedling adaptation and transplantation to obtain banana sprouts. Compared with the prior art, through a tissue culture technique, the breeding speed and theneatness of nursery stock can be greatly increased, the original female parent properties can be better maintained, the survival rate can be improved, thus, the output is increased, and the transplanting survival rate can reach 95%.

The invention provides a method for cutting crape myrtle seedlings on a seedbed and belongs to the technical field of plant cutting methods. The method is characterized by comprising the following steps of 1 setting up a greenhouse; 2 arranging the seedbed; 3 preparing cuttings; 4 performing cutting; 5 performing field management. By means of the method for cutting the crape myrtle seedlings on the seedbed, the variety number can be rapidly increased, female parent characters can be kept, the root quality and the growth situation of aboveground parts are good, the same-area cutting amount is large, and the cutting survival rate reaches to 93%.

The invention relates to a tissue culture method of nandina domestica firepower, and the method is based on newly germinated buds of nandina domestica firepower, and comprises the following steps: obtaining a sterile material, performing differentiation and proliferation of buds, performing strong seedling culture and rooting culture of adventitious buds, performing seedling hardening and seedling transplanting so as to finally obtain a nandina domestica firepower plant with good characters. Compared with the prior art, the invention has a transplanting survival rate of up to 95%, greatly increases the reproductive speed and the seedling uniformity, and maintains original female parent characters better.

The invention relates to a flaxbush tissue culture method. The method comprises the following steps: (1) preparing a culture medium comprising components and content of the culture medium in each stage during tissue culture, 1) a bud induction culture medium: MS+6-BA(1.0-5.0)mg / L+NAA(0.1-0.5)mg / L; 2) an adventitious bud proliferation culture medium: MS+6-BA(1.0-3.0)mg / L+NAA(0.1-0.3)mg / L; 3) a strong seedling culture medium: MS+6-BA(0.5-2.0)mg / L+NAA(0.1-0.2)mg / L; 4) a rooting culture medium: MS+NAA(0.05-0.2)mg / L, wherein the ph values of the culture mediums under various conditions is 5.8; adding 30g / L of cane sugar, 6g / L of agar, the culture temperature is 25+ / -1 DEG C, and the illumination is about 80 micromoles. Compared with the prior art, the flaxbush tissue culture method has the advantages that the breeding speed and the seedling uniformity can be greatly improved, and the original female parent characters are well kept.

The invention relates to a tissue culture method for heuchera micrantha 'Palace Purple'. The method comprises the following steps of: obtaining sterile materials; differentiating and proliferating buds; culturing strong seedlings of adventitious buds; rooting; and hardening off seedlings and transplanting the seedlings and the like. Compared with the prior art, the method greatly improves the reproductive speed of the heuchera micrantha 'Palace Purple' and the trimming of the seedlings, and improves the stability of shape and properties of the heuchera micrantha 'Palace Purple', so that the industrial mass production of the seedlings can be realized.

The invention relates to a method for in vitro culture of hemerocallis middendorfii Trautv. et Mey. tissue. The method comprises the following steps: picking a small bud on a flower stalk when hemerocallis middendorfii Trautv. et Mey. is in the blooming season, performing sterile processing on the small bud, and sequentially performing bud differentiation and proliferation, strong adventitious bud seedling culture, rooting culture, seedling exercising and transplanting to obtain the hemerocallis middendorfii Trautv. et Mey. Compared with the prior art, the year propagation coefficient of the hemerocallis middendorfii Trautv. et Mey. can be increased to 1: 100,000 or even higher, and the cost can be reduced to 0.5-0.8 yuan / strain, so that the reproduction rate and the uniformity of seedlings are greatly improved, and original female parent characters are better maintained.

The invention relates to a rapid breeding method based on tissue culture for ligularia kaempferi. The method comprises the following steps: (1) preparing a bud inducing culture medium, an adventitiousbud proliferation culture medium, a seedling strengthening culture medium, and a rooting culture medium; and (2) performing tissue culture on the ligularia kaempferi. According to the method providedby the invention, through a tissue culture technology the annual propagation coefficient is improved to 1:50000 and the cost is reduced by 0.8-1 yuan / plant compared with those of the prior art; and because the floral leaves of the leaves are a yellow-green chimera, the difficulty of the tissue culture is larger, through adjustment of the ratio of auxin to cytokinin, the characteristics of the leaves are obvious, the propagation speed and the seedling uniformity are greatly improved, and the original female parent characters are better maintained.

The invention relates to a tissue culture method of dianella ensifolia. The tissue culture method comprises the steps: respectively using a budlet induction medium for carrying out bud induction culture, a proliferation medium for carrying out proliferation culture, a strong seedling medium for carrying out strong seedling culture and a rooting medium for rooting culture, then carrying out domestication and transplanting so as to obtain dianella ensifolia plants. Compared with the prior art, the tissue culture method has the advantages that the reproduction speed and the evenness degree of seedlings are greatly increased through a tissue culture technology, the original female parent characters are maintained well, and the survival rate of transplanting can reach 95%.

The invention relates to a cultivating method of Hosta plantaginea Gold Standard. The method comprises the following steps of: first carrying out sterile treatment on Hosta plantaginea Gold Standard buds excavated in the early spring; and then inoculating the buds on a bud inducing culture medium to differentiate the buds; culturing the differentiated buds for 1 month; and then cutting off a budding callus and putting the budding callus into a proliferation medium to be proliferated so as to obtain cluster buds; after dividing the cluster buds into shoots or single buds, putting the shoots orthe single buds into a seedling culture medium to culture the seedling with adventitious buds; obtaining 2-3 cm small plants after 20 days; transferring the small plants into a rooting culture mediumto be induced for rooting; selecting strongly growing sterile seedlings with developed roots; carrying out seedling adaptation indoors in an open bottle for three days; washing off agars at the rootsof the seedlings after taking out the seedlings; transplanting the seedlings outdoors after naturalizing the seedlings in a greenhouse for 40 days; and carrying out fertilizer and water management onthe seedlings. Compared with the prior art, the cultivating method of the Hosta plantaginea Gold Standard provided by the invention has the following advantages that: the reproductive speed and the evenness of the nursery-grown plants can be largely increased; the original maternal character is preferably kept; and the survival rate can be increased so that the yield is increased and the transplanting survival rate can reach 95%.

The invention belongs to the technical field of asexual reproduction of wild ganoderma lucidum karst, and specifically relates to a cultivation method for wild ganoderma lucidum karst. The cultivationmethod includes ganoderma lucidum karst obtaining, mother strain preparation, original strain preparation, production strain cultivation and ganoderma lucidum karst fruit body cultivation; the raw materials of a mother strain culture medium include potatoes, rice milk, sugar, agar and water; the raw materials of an original strain culture medium include wood chips, platycodon grandiflorum powder,wheat bran, wheat, corns, gypsum, the sugar and calcium carbonate, with the balance being water; and the raw materials of a production strain culture medium include the wood chips, sweet potato flour, the wheat, the corns, soybean meal and the sugar, with the balance being water. The cultivation method utilizes the wild ganoderma lucidum karst as a material to reproduce strains indoors without limiting by seasons, so that quantity demands on production can be met, maternal characters can also be maintained, high-quality ganoderma lucidum karst strains suitable for local development can be effectively cultivated, and large-scale production of ganoderma lucidum karst industries can be realized; and the cultivation method has good economic, social and ecological benefits.

The invention discloses a quick cultivation method of Rosa multiflora thunb.var.cathayensis. a rooting powder solution is NAA with the concentration of 300 mg.L<-1>, red-yellow soil serves as a cutting medium, one-year-old stems with leaves serve as cuttings, treatment time is four hours, the cutting substrate which is 8-10 cm thick covers a seedbed, the processed cutting scions are inserted by being punched by a wood stick according to the plant spacing of 15 cm*15 cm, the insertion depth is 1 / 2-2 / 3 of the length of the scions, the soil is sufficiently watered immediately, and the temperature is 20-25 DEG C. The method is high in cutting survival rate and rooting rate and good in quality, and lays a foundation for large-scale seedling raising of Rosa multiflora thunb.var.cathayensis.

The invention relates to a method for culturing tissues of Buxus sempervives. The method comprises the following steps of: picking budlets on the upper part of the Buxus sempervives, and sequentially performing sterile treatment, differentiating and proliferating buds, performing hardening-off culture of adventitious buds, performing rooting culture, exercising seedlings and transplanting to obtain seedlings of the Buxus sempervives. Compared with the prior art, the method has the advantages that: by a tissue culture technology, the reproductive speed and the uniformity of nursery stocks can be greatly improved, the original maternal character can be better maintained, and the survival rate can be improved, so that the yield is improved; and the transplantation survival rate can reach 95 percent.

The invention provides a method for cutting crape myrtle seedlings on a seedbed and belongs to the technical field of plant cutting methods. The method is characterized by comprising the following steps of 1 setting up a greenhouse; 2 arranging the seedbed; 3 preparing cuttings; 4 performing cutting; 5 performing field management. By means of the method for cutting the crape myrtle seedlings on the seedbed, the variety number can be rapidly increased, female parent characters can be kept, the root quality and the growth situation of aboveground parts are good, the same-area cutting amount is large, and the cutting survival rate reaches to 93%.

The invention relates to a tissue culture method of Hemerocallis dumortieri, comprising the steps of: acquisition of bacteria-free material, differentiation and proliferation of bud, sound seeding of adventitious bud, rooting culture, hardening and transplantation and the like. Compared with the prior art, the tissue culture method greatly enhances reproduction speed of Hemerocallis dumortieri and uniformity of seedlings, better maintains original maternal character, and can realize batch production of seedlings in factory.

The invention relates to a tissue culture method of president clematis. Tender shoots of 'president' clematis are selected as a raw material; and a clematis 'president' plant is acquired through steps of an aseptic processing, a differentiation and propagation of tender shoots, a strong seedling culture of adventitious buds, a rooting culture of a root, a plant hardening seedling and transplanting, etc. Compared with a prior art, the invention employs tissue culture to increase a breeding speed of nursery stock and regularity of the seedlings, to better maintain original female parent properties, and to realize factory seedling growing, so as to guarantee market supply demand.

The invention relates to a breeding method of a new variety of banana, which comprises the five steps of sterile material acquisition, bud differentiation and proliferation, adventitious bud strong stock cultivation, rooting cultivation, and seedling adaptation and transplantation to obtain banana sprouts. Compared with the prior art, through a tissue culture technique, the breeding speed and theneatness of nursery stock can be greatly increased, the original female parent properties can be better maintained, the survival rate can be improved, thus, the output is increased, and the transplanting survival rate can reach 95%.

The invention relates to a method for cultivating gold-leaf sedge. The method comprises the following steps of: picking a budlet of gold-leaf sedge; and performing sterile pretreatment, differentiation and multiplication of the budlet, adventitious bud acclimatizing culturing, rooting culturing, seedling adaptation and transplanting to obtain a gold-leaf sedge plant. Compared with the prior art, the method has the advantages that: by adopting a tissue culturing technology, the reproduction speed and the regularity of nursery stock can be greatly increased, the original maternal character is better kept, and the survival rate can be increased, so that the yield is increased and the transplanting survival rate is up to 95 percent.

The invention discloses a sweetleaf root 'matcha' seedling culture method which includes the steps: digging sweetleaf root 'matcha' stem base tissues in spring and summer, performing bacteria-free treatment, and inoculating the tissues on a multiple-shoot inducement culture medium for multiple-shoot inducement culture to obtain multiple shoots 30 days later; dividing the multiple shoots and placing the multiple shoots into a proliferation culture medium for proliferation culture with the cycle of 30 days; acquiring a certain number of multiple shoots by proliferation culture, dividing the multiple shoots into single bud seedlings, culturing the bud seedlings in a rooting culture medium for rooting induction, and forming a complete plant 25-30 days later; hardening the seedlings with a bottle cap in an environment with scattered light for 3-7 days, and then hardening the seedlings in an uncapped manner for 1-2 days; taking out test-tube seedlings after hardening, washing a root culturemedium and transplanting the seedlings into a seedling culture plug. Compared with the prior art, the sweetleaf root 'matcha' seedling culture method has the advantages that sweetleaf root 'matcha' seedlings are reproduced by a tissue culture technology, operation is simple, reproductive rate is high, the problem of too low speed of division propagation can be solved, and mass production of the sweetleaf root 'matcha' seedlings with consistent characters is realized.

The invention discloses a method for the tissue culture of long-ear kniphofia. The method comprises the steps of picking up long-ear kniphofia budlet, washing, culturing by a bud induction medium, putting cultured calluses with buds into a multiplication medium for differentiation multiplication, carrying out adventitious bud cultivation and rooting cultivation, and obtaining the long-ear kniphofia plant through the seedling hardening technology and the transplanting technology. Compared with the prior art, according to the tissue culture technology, the maturing rate can be improved, and the different nutrition facts can be provided at the different cultivation stages by the selected bud induction culture medium, multiplication culture medium, seedling boosting culture medium and rooting culture medium, so that the original female characters can be kept to the maximum, and the transplanting survival rate in the steps of seedling hardening and transplanting can reach 90%.

The invention relates to a method for culturing red-petiole taros. The method comprises the following steps: (1) obtaining sterile material; (2) differentiating and proliferating buds; (3) culturing adventitious bud strong seedlings; (4) striking roots and culturing; and (5) acclimatizing and transplanting. Compared with the prior art, the method has the advantages that the propagation speed and the neatness of seedlings can be greatly increased through a tissue culture technology, the trait of a female patient is better retained, and the survival rate can be increased, therefore, the yield is increased, and the transplanting survival rate can be up to 95%.

The invention belongs to the technical field of asexual propagation of wild ganoderma, and in particular relates to a cultivation method of wild ganoderma. A cultivation method of wild Ganoderma lucidum, comprising the acquisition of Ganoderma lucidum entities, preparation of mother seeds, preparation of original seeds, cultivation of production species and cultivation of fruiting bodies of Ganoderma lucidum, wherein the raw materials of the mother seed culture medium include potatoes, rice milk, sugar, agar, and water; the original seeds The medium raw materials include sawdust, bellflower powder, wheat bran, wheat, corn, gypsum, sugar, calcium carbonate, and the balance is water; the production medium raw materials include sawdust, sweet potato powder, wheat, corn, soybean flour, sugar, and the balance It is water; the present invention uses wild ganoderma as a material to propagate strains indoors without seasonal restrictions, which can not only meet the quantity demand in production, but also maintain the traits of the female parent, and effectively select and breed high-quality ganoderma suitable for local development species, the large-scale production of Ganoderma lucidum industry has been realized, and it has good economic, social and ecological benefits.

The invention belongs to the field of tissue culture of medicinal plants, and discloses an amomum tsao-ko tissue culture and seedling raising method. The amomum tsao-ko tissue culture and seedling raising method comprises the following steps of: selecting an explant: selecting the young stem segment at the base of an amomum tsao-ko stem; disinfecting the explant; performing primary induction culture; performing secondary enrichment culture; and performing rooting culture. According to the amomum tsao-ko tissue culture and seedling raising method disclosed by the invention, the adopted explantis the young stem segment at the base of the stem; compared with young shoots near the soil surface adopted at the beginning of an experiment, the contamination rate is decreased to 12% at the young stem segment from 95% at the young shoots near the soil surface; but, the inductivity of the young stem segment at the base of the stem is 80%, and slightly lower than the inductivity, namely, 84%, atthe young shoots near the soil surface.

The invention relates to a plant culturing method of spot Farfugium. According to the method, the spot Farfugium plant is obtained by a tissue culture technology; and the specific steps comprise asepsis, differentiation and proliferation, seedling cultivating, rooting culture as well as seedling adaptation and transplantation. Compared with the prior art, the plant culturing method of the spot Farfugium provided by the invention has the advantage of increasing the reproductive speed of the plants through the tissue culture technology so that the market requirements can be satisfied. In addition, by using the method, the original maternal character can be kept and the evenness of the plants is also higher.

The invention relates to a tissue culture method for heuchera micrantha 'Palace Purple'. The method comprises the following steps of: obtaining sterile materials; differentiating and proliferating buds; culturing strong seedlings of adventitious buds; rooting; and hardening off seedlings and transplanting the seedlings and the like. Compared with the prior art, the method greatly improves the reproductive speed of the heuchera micrantha 'Palace Purple' and the trimming of the seedlings, and improves the stability of shape and properties of the heuchera micrantha 'Palace Purple', so that the industrial mass production of the seedlings can be realized.

The invention relates to a flaxbush tissue culture method. The method comprises the following steps: (1) preparing a culture medium comprising components and content of the culture medium in each stage during tissue culture, 1) a bud induction culture medium: MS+6-BA(1.0-5.0)mg / L+NAA(0.1-0.5)mg / L; 2) an adventitious bud proliferation culture medium: MS+6-BA(1.0-3.0)mg / L+NAA(0.1-0.3)mg / L; 3) a strong seedling culture medium: MS+6-BA(0.5-2.0)mg / L+NAA(0.1-0.2)mg / L; 4) a rooting culture medium: MS+NAA(0.05-0.2)mg / L, wherein the ph values of the culture mediums under various conditions is 5.8; adding 30g / L of cane sugar, 6g / L of agar, the culture temperature is 25+ / -1 DEG C, and the illumination is about 80 micromoles. Compared with the prior art, the flaxbush tissue culture method has the advantages that the breeding speed and the seedling uniformity can be greatly improved, and the original female parent characters are well kept.

The invention relates to a tissue culture method of baptisia australis. The tissue culture method comprises the steps of sterile treatment, differentiation and proliferation, strong seedling of adventitious buds, rooting culture, acclimatization and transplanting, and the like. The step-by-step tissue culture of the baptisia australis is carried out by using a bud induction medium, a proliferation medium, a strong seedling medium and a rooting medium. Compared with the prior art, the tissue culture method has the advantages that the baptisia australis is subjected to step-by-step tissue culture by selecting the appropriate mediums, so that the reproduction speed and the evenness degree of nursery stocks are increased, the original female parent characters are maintained well, and in addition, the survival rate of transplanting can reach 85%.

The invention belongs to the technical field of forestry reproduction and discloses a reproduction and transplantation regulator for reddening leaves of acer palmatum and a reproduction and planting method of the acer palmatum. The reproduction and transplantation regulator is prepared from components as follows: indolebutyric acid, magnesium carbonate, ferric phosphate, zinc sulfate, aluminum sulfate, ammonium nitrate and potassium stearate. All that is required for large-scale planting is to rapidly dip cuttings in the regulator, and the reproduction and transplantation regulator is particularly suitable for being used in gardens, municipal engineering, roads and landscape industries. The reproduction and transplantation regulator can make acer palmatum have uniform colors, redder leaves and higher ornamental value, leaf yellowing, leaf spots and powdery mildew are prevented and treated, the cutting survival rate is high, and the cutting number is large in the same area.

The invention discloses a quick cultivation method of Rosa multiflora thunb.var.cathayensis. a rooting powder solution is NAA with the concentration of 300 mg.L<-1>, red-yellow soil serves as a cutting medium, one-year-old stems with leaves serve as cuttings, treatment time is four hours, the cutting substrate which is 8-10 cm thick covers a seedbed, the processed cutting scions are inserted by being punched by a wood stick according to the plant spacing of 15 cm*15 cm, the insertion depth is 1 / 2-2 / 3 of the length of the scions, the soil is sufficiently watered immediately, and the temperature is 20-25 DEG C. The method is high in cutting survival rate and rooting rate and good in quality, and lays a foundation for large-scale seedling raising of Rosa multiflora thunb.var.cathayensis.