A kind of tissue culture method of New Zealand hemp

A tissue culture and tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of limited seedlings, small number of introduced species, large market demand, etc., achieve good maternal traits, improve seedlings the uniformity and the effect of improving reproduction speed

Active Publication Date: 2016-08-24
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the small number of species introduced from abroad, their propagation is slow, the market demand is large, and the supply of seedlings is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for tissue culture of New Zealand hemp, the method comprising the following steps:

[0035] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0036] 1) Bud induction medium: MS+6-BA5mg / L+NAA5mg / L;

[0037] 2) Adventitious bud proliferation medium: MS+6-BA3mg / L+NAA0.3mg / L;

[0038] 3) Strong seedling medium: MS+6-BA0.1mg / L+NAA0.1mg / L;

[0039] 4) Rooting medium: MS+NAA0.1mg / L;

[0040] The pH of the above-mentioned medium is 5.8, 30g / .L of sucrose, 6g / L of agar are added, the culture temperature is (25±1)℃, and the light is 80μmol / m -2 About s:

[0041] (2) Tissue culture of New Zealand hemp

[0042] 1) Obtaining sterile materials

[0043] Dig out the small buds at the base of the New Zealand hemp plant, wash them with tap water for 2 hours, put them on an ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, wash ...

Embodiment 2

[0053] A method for tissue culture of New Zealand hemp, the method comprising the following steps:

[0054] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0055] 1) Bud induction medium: MS+6-BA1.0mg / L+NAA0.1mg / L;

[0056] 2) Adventitious bud proliferation medium: MS+6-BA1mg / L+NAA0.1mg / L;

[0057] 3) Strong seedling medium: MS+6-BA0.5mg / L+NAA0.1mg / L;

[0058] 4) Rooting medium: MS+NAA0.05mg / L;

[0059] The pH of the above-mentioned medium is 5.8, add 30g / .L sucrose, 6g / L agar, culture temperature is (25±1)℃, light is 70μmol / m -2 About s:

[0060] (2) Tissue culture of New Zealand hemp

[0061] 1) Obtaining sterile materials

[0062] Dig out the small buds at the base of the New Zealand hemp plant, wash them with tap water for 2 hours, put them on an ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, wash them with sterile w...

Embodiment 3

[0072] A method for tissue culture of New Zealand hemp, the method comprising the following steps:

[0073] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0074] 1) Bud induction medium: MS+6-BA3.0mg / L+NAA0.3mg / L;

[0075] 2) Adventitious bud proliferation medium: MS+6-BA2mg / L+NAA0.2mg / L;

[0076] 3) Strong seedling medium: MS+6-BA2mg / L+NAA0.2mg / L;

[0077] 4) Rooting medium: MS+NAA0.2mg / L;

[0078] The pH of the above-mentioned medium is 5.8, add 30g / .L sucrose, 6g / L agar, culture temperature is (25±1)℃, light is 90μmol / m -2 About s:

[0079] (2) Tissue culture of New Zealand hemp

[0080] 1) Obtaining sterile materials

[0081] Dig out the small buds at the base of the New Zealand hemp plant, wash them with tap water for 2 hours, put them on an ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, wash them with sterile wate...

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PUM

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Abstract

The invention relates to a flaxbush tissue culture method. The method comprises the following steps: (1) preparing a culture medium comprising components and content of the culture medium in each stage during tissue culture, 1) a bud induction culture medium: MS+6-BA(1.0-5.0)mg / L+NAA(0.1-0.5)mg / L; 2) an adventitious bud proliferation culture medium: MS+6-BA(1.0-3.0)mg / L+NAA(0.1-0.3)mg / L; 3) a strong seedling culture medium: MS+6-BA(0.5-2.0)mg / L+NAA(0.1-0.2)mg / L; 4) a rooting culture medium: MS+NAA(0.05-0.2)mg / L, wherein the ph values of the culture mediums under various conditions is 5.8; adding 30g / L of cane sugar, 6g / L of agar, the culture temperature is 25+ / -1 DEG C, and the illumination is about 80 micromoles. Compared with the prior art, the flaxbush tissue culture method has the advantages that the breeding speed and the seedling uniformity can be greatly improved, and the original female parent characters are well kept.

Description

technical field [0001] The invention relates to plant cultivation, in particular to a tissue culture method of New Zealand hemp. Background technique [0002] New Zealand hemp is a shrub plant of the Agaveaceae New Zealand genus Hemp, which is a perennial evergreen herb. New Zealand hemp has no central erect stem, but has short, fleshy, creeping, forked running stems, about 5 cm in diameter. A group of strong, sword-shaped leaves emerge from the walking stems, growing slightly fan-shaped, strong, thick leathery, and basal. The panicle is born on the leafless flower stem, the base of the flower is cylindrical, the corolla is dark red, and it blooms in summer. Capsule erect, with three edges. It likes a warm and sunny environment. It is suitable for growth in deep, humus-rich sandy loam. It is not cold-resistant. It is more suitable for garden cultivation or flower border configuration. It is an excellent foliage plant with beautiful plant shape, gorgeous and elegant colors...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄建荣张浪陈建华沈勤
Owner 上海上房园艺有限公司
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