Breeding method of new variety of banana

A banana and culture medium technology, applied in the field of tissue culture of herbaceous plants, to achieve the effect of increasing the reproduction speed, increasing the survival rate, and increasing the yield

Active Publication Date: 2013-03-06
上海上房园艺有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture report of banana 'Rowe Red' has not been seen yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for cultivating a new banana variety, the method comprising the following steps:

[0027] (1) Aseptic treatment

[0028] Dig out the small buds at the base of the banana, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 30s, soak them in 1wt‰ mercury solution for 15mins, wash them with sterile water for 5-6 times, and use them Blot the surface moisture with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA2.0mg / L+NAA0.2mg / L;

[0029] (2) Differentiation and proliferation of buds

[0030] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA1.0mg / L+NAA0.1mg / L was cultured, a...

Embodiment 2

[0038] A method for cultivating a new banana variety, the method comprising the following steps:

[0039] (1) Aseptic treatment

[0040] Dig out the small buds at the base of the banana, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 20s, soak them in 1wt‰ mercury chloride solution for 10mins, rinse them with sterile water for 5-6 times, and use them. Blot the surface moisture with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA1.0mg / L+NAA0.1mg / L;

[0041] (2) Differentiation and proliferation of buds

[0042] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA2.0mg / L+NAA0.2mg / L was ...

Embodiment 3

[0050] A method for cultivating a new banana variety, the method comprising the following steps:

[0051] (1) Aseptic treatment

[0052] Dig out the small buds at the base of the banana, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 40s, soak them in 1wt‰ mercury chloride solution for 20mins, rinse them with sterile water for 5-6 times, and use them. Blot the surface moisture with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA3.0mg / L+NAA0.3mg / L;

[0053] (2) Differentiation and proliferation of buds

[0054]Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA3.0mg / L+NAA0.3mg / L was c...

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PUM

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Abstract

The invention relates to a breeding method of a new variety of banana, which comprises the five steps of sterile material acquisition, bud differentiation and proliferation, adventitious bud strong stock cultivation, rooting cultivation, and seedling adaptation and transplantation to obtain banana sprouts. Compared with the prior art, through a tissue culture technique, the breeding speed and theneatness of nursery stock can be greatly increased, the original female parent properties can be better maintained, the survival rate can be improved, thus, the output is increased, and the transplanting survival rate can reach 95%.

Description

technical field [0001] The invention relates to a method for tissue culture of herbaceous plants, in particular to a method for cultivating a new variety of banana. Background technique [0002] Banana 'Rowe Red' is a large herbaceous plant, about 1 meter high, with red stripes on the leaves, which is unique for ornamental purposes. It is a small plant in the Banana genus, and it is a plant material mainly for foliage. Bananas are primarily cultivated in the tropics, but are now also valued in temperate regions. As the species introduced from abroad, the number of introductions is small, and its ramets are slow, but there is a fixed market demand, and the market demand is large, and the supply of seedlings is limited. Through tissue culture technology, the propagation speed and the uniformity of seedlings are greatly improved, and the original female parent traits are better maintained. The tissue culture report of banana 'Rowe Red' has not been seen yet. Contents of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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