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Tissue culture method of nandina domestica firepower

A technology of nandina and flame, which is applied in the field of tissue culture of nandina 'flame', can solve the problems of cutting, slow branching, low cutting survival rate and small number, etc., and achieve the effect of increasing the speed of reproduction and

Active Publication Date: 2013-03-06
上海上房园艺有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this plant is a variety introduced from abroad, and there is no report on tissue culture, so the number is small, its cuttings and divisions are slow, and the survival rate of cuttings is low, resulting in limited supply of seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The tissue culture method of nandina 'flame', the method comprises the following steps:

[0029] (1) Obtaining sterile materials

[0030] Pick the newly germinated buds of Nantian bamboo, rinse them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 20s and 1wt‰mercuric chloride solution for 10mins, rinse them with sterile water for 5-6 times and remove them. Blot the surface moisture, take the base of the buds and cut them into 1cm and inoculate them in the bud induction medium. The composition of the bud induction medium is MS+6-BA1.0mg / L+NAA0.1mg / L+IBA1.0mg / L L;

[0031] (2) Differentiation and proliferation of buds

[0032] Four weeks after inoculation of the small buds on the small bud induction medium, the small buds began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks. Proliferation, adventitious buds grow rapidly without vitrification and other adverse phenom...

Embodiment 2

[0041] The tissue culture method of nandina 'flame', the method comprises the following steps:

[0042] (1) Obtaining sterile materials

[0043] Pick the newly germinated buds of Nantian bamboo, rinse them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 40s and 1wt‰mercuric chloride solution for 20mins, rinse them with sterile water for 6 times and blot them dry Surface water, take the base of the budlet and cut it into 1cm and inoculate it in the budlet induction medium, the composition of the budlet induction medium is MS+6-BA3.0mg / L+NAA0.3mg / L+IBA3.0mg / L;

[0044] (2) Differentiation and proliferation of buds

[0045] Four weeks after inoculation of the small buds on the small bud induction medium, the small buds began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks. Proliferation, adventitious buds grow rapidly without vitrification and other adverse phenomena, they gr...

Embodiment 3

[0054] The tissue culture method of nandina 'flame', the method comprises the following steps:

[0055] (1) Obtaining sterile materials

[0056] Pick the newly germinated buds of Nantian bamboo, rinse them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 30s and 1wt‰mercuric chloride solution for 15mins, rinse them with sterile water for 5 times and blot them dry Surface water, take the base of the budlet and cut it into 1cm and inoculate it in the budlet induction medium, the composition of the budlet induction medium is MS+6-BA5.0mg / L+NAA0.5mg / L+IBA5.0mg / L;

[0057] (2) Differentiation and proliferation of buds

[0058] 4 weeks after the buds were inoculated on the bud induction medium, the buds began to swell, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks, and there were more small buds on the callus at the base, but it did not affect the adventitious buds Adventitious buds grow ...

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PUM

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Abstract

The invention relates to a tissue culture method of nandina domestica firepower, and the method is based on newly germinated buds of nandina domestica firepower, and comprises the following steps: obtaining a sterile material, performing differentiation and proliferation of buds, performing strong seedling culture and rooting culture of adventitious buds, performing seedling hardening and seedling transplanting so as to finally obtain a nandina domestica firepower plant with good characters. Compared with the prior art, the invention has a transplanting survival rate of up to 95%, greatly increases the reproductive speed and the seedling uniformity, and maintains original female parent characters better.

Description

technical field [0001] The invention relates to a method for tissue culture of plants, in particular to a method for tissue culture of nandina 'flame'. Background technique [0002] Nandina flame is a small evergreen shrub that likes a warm, humid and well-ventilated semi-shady environment. Cold-resistant, drought-resistant, able to tolerate slightly alkaline soil, suitable for growth in sandy loam containing humus. Nandina flame has a compact plant type, wider leaves than ordinary nandina, bright red leaves under strong light, especially in mid-winter season, the leaf color is very bright. In the garden, it is often planted in clusters or isolated in flower borders and courtyards, and can also be used as a ground cover plant in sparse forests. It is a good flower mirror and garden material, and has a large market demand. However, this plant is a species introduced from abroad, and there is no report on tissue culture, so the quantity is small, its cuttings and divisions a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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