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Plant culturing method of spot Farfugium

A spot and culture medium technology, which is applied in the field of plant cultivation of the spot Aurea spp., can solve the problems of slow branching, limited supply of seedlings, and small number of introduced species, and achieve high uniformity, meet market demand, and increase reproduction speed. Effect

Active Publication Date: 2013-03-06
上海上房园艺有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because it is a variety imported from abroad, the number of introduced species is small, and the ramets are slow, resulting in limited supply of seedlings and unable to meet market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The plant culture method of Pseudomonas vulgaris, the tissue culture method includes five steps of aseptic treatment, differentiation and proliferation, cultivation of strong seedlings, cultivation of rooting and cultivation of seedlings, and the specific steps are as follows:

[0024] (1) Aseptic treatment: Rinse the small buds of Pseudomonas auratus with tap water for 2 hours, then place them on the ultra-clean workbench, soak them in ethanol with a concentration of 75% for 20s, soak them in 1‰ mercuric chloride for 10 minutes, and rinse with sterile water for 5 hours. At the same time, use sterile filter paper to absorb the surface moisture, cut the base of the small buds into 1cm lengths and inoculate them on the bud induction medium. L, agar 6g / L, the main nutrients are MS+6-BA1.0mg / L+NAA0.1mg / L, the pH value of the medium is 5.6;

[0025] (2) Differentiation and proliferation: The small buds were inoculated on the bud induction medium for 3 weeks and began to expa...

Embodiment 2

[0030] The plant culture method of Pseudomonas vulgaris, the tissue culture method includes five steps of aseptic treatment, differentiation and proliferation, cultivation of strong seedlings, cultivation of rooting and cultivation of seedlings, and the specific steps are as follows:

[0031] (1) Aseptic treatment: Rinse the small buds of Pseudomonas vulgaris with tap water for 2 hours, then place them on the ultra-clean workbench, soak them in ethanol with a concentration of 75wt% for 30s, soak in mercuric chloride with a concentration of 1wt‰ for 15min, and rinse with sterile water for 6 hours. At the same time, use sterile filter paper to dry the surface moisture, cut the base of the small buds into 1cm length and inoculate on the bud induction medium, control the culture temperature to be 25 ° C, the light condition is 80 μmol / ms, and the standard component of the medium is sucrose 30 g / L, agar 6g / L, the main nutrients are MS+6-BA3.0mg / L+NAA0.3mg / L, the pH value of the med...

Embodiment 3

[0037] The plant culture method of Pseudomonas vulgaris, the tissue culture method includes five steps of aseptic treatment, differentiation and proliferation, cultivation of strong seedlings, cultivation of rooting and cultivation of seedlings, and the specific steps are as follows:

[0038](1) Aseptic treatment: Rinse the small buds of Pseudomonas vulgaris with tap water for 2 hours, then place them on the ultra-clean workbench, soak them in ethanol with a concentration of 75% for 30s, soak them in 1‰ mercuric chloride for 15 minutes, and rinse them with sterile water for 6 hours. At the same time, use sterile filter paper to absorb the surface moisture, cut the base of the small buds into 1cm lengths and inoculate them on the bud induction medium. L, agar 6g / L, the main nutrients are MS+6-BA5.0mg / L+NAA0.5mg / L, the pH value of the medium is 5.9;

[0039] (2) Differentiation and proliferation: The small buds were inoculated on the bud induction medium for 3 weeks and began to...

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PUM

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Abstract

The invention relates to a plant culturing method of spot Farfugium. According to the method, the spot Farfugium plant is obtained by a tissue culture technology; and the specific steps comprise asepsis, differentiation and proliferation, seedling cultivating, rooting culture as well as seedling adaptation and transplantation. Compared with the prior art, the plant culturing method of the spot Farfugium provided by the invention has the advantage of increasing the reproductive speed of the plants through the tissue culture technology so that the market requirements can be satisfied. In addition, by using the method, the original maternal character can be kept and the evenness of the plants is also higher.

Description

technical field [0001] The invention relates to a tissue culture method of plants, in particular to a plant culture method of Pseudomonas spp. Background technique [0002] Spotted Dawufengcao is an evergreen perennial herb with thick rhizomes and a stem height of 30 to 70 cm. The leaves are mostly basal, bright green, densely covered with star-like macular, kidney-shaped, wavy edges, and loose flower heads. Umbrella-shaped, yellow, blooming in late autumn, prefers semi-shade and humid environment; cold-resistant, can overwinter in the open field in the south of the Yangtze River; afraid of direct sunlight; good soil adaptability, fertile, loose, well-drained loam is appropriate. This variety is widely used in gardens. Many pieces are planted as forest ground cover, or planted in flower borders or courtyards. It is an excellent sparse forest ground cover plant, and it is also a good flower mirror and courtyard material. Because it is a variety introduced from abroad, the nu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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