254 results about "Aseptic processing" patented technology

A method for implanting a breast implant into a subject, the method includes: providing a sterile, flexible, elastic biodegradable bag sized to contain the breast implant; providing a sterile breast implant; inserting, using sterile handling, the sterile breast implant into the sterile bag to form a sterile breast implant assembly; closing the bag to fully enclose the implant within the bag; and implanting in a sterile manner the sterile breast implant assembly into the subject. A sterile bag, which may be provided in a kit, preferably for use with the method includes first and second chambers connected by a channel for moving a breast implant from the first chamber through the channel to the second chamber without directly touching the implant. An incision dilator may also be included with the kit or separately provided for use in a preferred embodiment of the method.

The invention provides multifunctional pure natural mouthwash and a preparation method thereof. The mouthwash contains sweetening agent, acidity agent, wetting agent, essence, alcohol, 0.5-1% of active constituent stabilizing agent, 0.01-0.3% of active constituent modifier and 0.05-0.1% of the active constituent deionized water, wherein the active constituents are at least two of tea polyphenol, propolis and Magnolia officinalis. The preparation method comprises the following steps of: dissolving the sweetening agent and the acidity agent into water, and adding with the wetting agent to form water-soluble cleaning liquid; and dissolving the active constituents into the alcohol, adding with the active constituent stabilizing agent, the essence and the active constituent modifier, and stirring to form oily solution; slowly adding the oily solution into the cleaning liquid, evenly mixing, statically putting for a night, filtering, and aseptically processing to obtain final products. Because of the combined action of the active constituents, the multifunctional pure natural mouthwash has an effect for multiply preventing and curing oral problems, such as decayed tooth, and has the functions of diminish inflammation and sterilization, desensitization, hemostasis, breath freshening, etc.

The present invention is directed to providing a sterile container protecting various surgical grafts, implants and devices from incidental contamination and damage during operative procedures. The design includes an inner box to contain the items, and an outer box to protect the exterior of the inner box from contamination in the event of fall or other contamination, such as from airborne particles or liquids, fall from the sterile field, or by non-sterile handling. In the event of fall or contamination of the outer box, the surgical staff will be able to recover the inner box and its contents in sterile and unspoiled condition without difficulty.

The invention relates to a medical compound micropore polysaccharide and application in hemostasis on wound surface bleeding areas of various wounds and operation tissues. The medical compound micropore polysaccharide is prepared by adopting a method with the following steps: (1) preparing starch fluid; (2) preparing carboxymethyl chitosan solution; (3) mixing the starch fluid and the carboxymethyl chitosan solution, and adding dispersant and emulsifier into the mixture; (4) emulsifying, crosslinking and co-polymerizing; and (5) refining, drying, packing and sterile treatment. The invention also discloses application of the medical compound micropore polysaccharide in preparing a hemostasis medicament used for the wound surface bleeding areas of wounds and operation tissues. The medical compound micropore polysaccharide of the invention has the following characteristics: 1, the hemostasis time is short, namely the hemostasis is generally finished in 3 to 5 minutes; 2, the medical compound micropore polysaccharide has no antigenicity, has polysaccharide components, and has no protein or immune reaction; 3, the medical compound micropore polysaccharide can be fully degraded and absorbed in vivo; 4, the medical compound micropore polysaccharide is a sterile product and is convenient to use by opening; and 5, the tissue reaction is light. Therefore, the medical compound micropore polysaccharide is a comparatively ideal surgical hemostasis product.

The invention relates to a soil covering culture method for simulated wild glossy ganoderma, which comprises the following steps that: a glossy ganoderma culture bag is subjected to germicidal treatment, inoculated with strains of the glossy ganoderma when the temperature in the culture bag is cooled to about 30 DEG C, and then cultured under dark or weak light environment, and mycelia are grown full of the bag; a furrow is made on loosen sandy soil, drainage channels are arranged on the periphery, a glossy ganoderma bed is dug in the furrow, the culture bag is opened and vertically arranged in the glossy ganoderma bed in a way that the inoculation surface is upwards, the bag is covered with soil, gaps are filled by the soil, and the soil is kept moist; the glossy ganoderma bag is cultured continuously in the open air and promoted to grow the glossy ganoderma naturally; and a sunshade is erected after the fruit body of the glossy ganoderma is ripen, a rainproof plastic film is spread on the sunshade, the culture is continued, and ganoderma lucidum spore is collected. The method adopts a field open cold frame soil covering mode to grow the glossy ganoderma, changes the problem that the glossy ganoderma is always in the greenhouse environment in the prior art, reduces cost, only needs aseptic processing and humidity maintaining in the culture process, and is simple, high in programming and easy in promotion.

The invention discloses a Paenibacillus kribbensis, and a preparation method and an application thereof. The Paenibacillus kribbensis has a bacterial code name of TRCC82001, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCCNo.7996. The above bacterial strain has the advantages of good production property, good metabolite antimicrobial effect, and strong activity against rice blast and sheath blight, and a solution obtained after diluting a broth subjected to antibiotic treatment to 100 times has an inhibition effect of above 80% on the rice blast and sheath blight. The bacterial strain and its metabolites have a broad antimicrobial spectrum, can be used for preparing biocontrol preparations for the broad spectrum activity inhibition of pathogenic fungi to control the fungal diseases of plants.

The invention discloses a turbid liquor eye composition with chlotiponuo and jiatishaxing and making method, which comprises the following parts: (a) 0. 2-2% chlotiponuo and salt with grain size below 50um, (b) 0. 2-1% jiatishaxing and salt, (c) 0. 5-2. 5% one or more non-ion polymer as suspension-assisting agent, (d) eye excipient agent. The making method comprises the following steps: grinding the chlotiponuo carbonate below 50um; sterilizing; adding jiatishaxing, non-ion polymer and other eye adjuvants to form an eye agent to treat and prevent opthalmitis and bacterial infection.

The present invention is directed to an environmentally closed cell processing device, for the aseptic processing of blood cells. The device includes a continuous flow centrifuge, fluid reservoirs and fluid handling systems. Blood cells are processed by the present device, to remove their immunodominant antigens. Seroconverted cells and methods of treating subjects with these cells are also described.

The invention discloses a method for producing a Fujian jewel orchid by plant tissue culture, which comprises the following three procedures: selection and sterile treatment of plants, induced culture of cluster buds and large-scale culture. The method is technically characterized in that two different culture media are used in the induced culture procedure and the large-scale culture procedure, wherein the culture medium for the induced culture comprises an MS culture medium, sucrose of 10-50g/L, agar of 6-8g/L, activated carbon of 2.0-3.0g/L, naphthylacetic acid of 0.1-3.0mg/L and 6-benzylaminopurine of 0.1-5.0mg/L, and the pH value is 5.0-7.0; and the culture medium for the large-scale culture comprises a modified MS culture medium, calcium nitrate of 600mg/L, mashed banana of 30-50g/L, sucrose of 20-40g/L, agar of 6-8g/L, activated carbon of 2.0-3.0g/L, naphthylacetic acid of 1.0-2.0mg/L and 6-benzylaminopurine of 2.0-3.0mg/L, and the pH value is 5.0-7.0. Plant tissue culture can keep the good characters of the plants. The method has the advantages of easy operation, low production cost, high yield, good quality and no environment pollution, is suitable for industrial seedling raising and can realize mass production.

The invention discloses a method for producing anoectochilus formosanus by tissue culture, comprising the following steps: frond selection and asepsis, cluster bud inducing culture and large-scale culture. The key to the technique is characterized in that two different culture media are applied to the inducing culture and the large-scale culture, wherein the inducing culture medium is as follows: H culture medium+ 10-50g/L of cane sugar + 0.1-3.0mg/L of alpha-naphthylacetic acid+ 0.1-5.0mg/L of 6-benzyladenine, and pH value is 5.0-7.0; the large-scale culture medium is as follows: B5 culture medium+ 20-40g/L of cane sugar+ 6-8g/L of agar+ 2.0-3.0g/L of active carbon+ 1.0-2.0mg/L of alpha-naphthylacetic acid+ 2.0-3.0mg/L of benzyladenine, and pH value is 5.5-6.5. The method is easy to operate, features low production cost, high output, high quality and no pollution to the environment and can realize batch production.

Machine for the aseptic treatment of containers (10) in bottling plant, of the rotary type provided with a plurality of stations (9) for treating the containers and so shaped as to cause the treatment of the containers to take place under substantially sterile conditions, is characterised in that it comprises sealing means that separate a non sterile area (11) of the machine from an area (12) maintained under sterile conditions and in which the treated containers (10) transit, said means comprising a fixed annular channel (15) at least partially filled with liquid in which slides a concentric annular element (16) associated with the rotating part (11).

The method of aseptically treating containers in accordance with the invention comprises the step of subjecting the containers while held upside-down in a heat treatment station to steam at 140° C. for 2 s to 20 s and then in subjecting the containers in a station to a disinfectant mixture comprising peracetic acid, hydrogen peroxide, acetic acid, and water raised to a maximum temperature of 60° C.

A flanged joint system connects two pipes disposed along a common axis. A flange having a mating surface with a circumferential groove terminates each pipe. A gasket assembly comprising an outer annular, substantially rigid retaining ring and an inner deformable annular sealing member is interposed between the mating surfaces of the flanges. The flanges are clamped together with their mating surfaces in axially aligned, facing relationship. The retaining ring limits the impingement of the flanges and prevents overcompression of the deformable material. The joint is reliable and durable, and permits aseptic processing conditions to be maintained. Servicing and gasket replacement are readily accomplished.

The invention relates to a tissue culture method of president clematis. Tender shoots of 'president' clematis are selected as a raw material; and a clematis 'president' plant is acquired through steps of an aseptic processing, a differentiation and propagation of tender shoots, a strong seedling culture of adventitious buds, a rooting culture of a root, a plant hardening seedling and transplanting, etc. Compared with a prior art, the invention employs tissue culture to increase a breeding speed of nursery stock and regularity of the seedlings, to better maintain original female parent properties, and to realize factory seedling growing, so as to guarantee market supply demand.

The invention discloses a method for realizing excellent plant body induction and successive propagation culture on dendrobium candidum by virtue of plant tissue culture. The method comprises three links: selecting a plant and sterilizing, inducing and culturing cluster buds and carrying out propagation culture in a large scale; and the method is characterized in that two different culture mediums are adopted in the two stages of inducing and culturing as well as culturing in the large scale. The method disclosed by the invention is easier to operate, the production cost is low, the method is applicable to industrial seed culturing, the yield is high, the quality is good, no pollution is produced to the environment, and batch production can be realized.

The present invention provides processes for aseptically processing live mammalian cells in an aqueous medium to produce a cell suspension having a cell density of at least about 10 million cells/mL and cell viability of at least about 90%. These methods comprise a step of reducing the volume of the medium using a tangential flow filter (TFF) having a pore size of greater than 0.1 micron, during which step the trans-membrane pressure (TMP) is maintained at less than about 3 psi and the shear rate is maintained at less than about 4000 sec⁻¹. The invention also provides a complete process for large scale manufacturing mammalian cells for use in a therapeutic composition, and scalable, fully disposable systems for carrying out the process, using readily available disposables and pumps.

The invention provides a method for preparing a decidua mesenchymal stem cell. The method comprises the following steps: performing placenta asepsis by taking the placenta of a male full-term fetus as a raw material; separating a decidual tissue; identifying the decidual tissue and identifying whether the decidua mesenchymal stem cell comes from a maternal tissue; culturing the decidua mesenchymal stem cell; freeze-storing the decidua mesenchymal stem cell; and reviving the decidua mesenchymal stem cell. The method provided by the invention has the technical characteristics that the sampled fetus is male fetus placenta; after the decidual tissue is obtained, sex determination is performed to identify whether the decidual tissue comes from a parent body and is free of pollution of a daughter tissue; by adopting a bacterial pollution prevention method, the pollution possibility is reduced from a sampling source; the surface is washed for a plurality of times, so that the pollution possibility is effectively reduced; a single enzyme is used for digestion to simplify the procedures; a serum-free medium is used for culture to reduce the use of an animal source component; the cell is stable in performance; a long-term in-vitro culture process of the decidua mesenchymal stem cell can be maintained; and the cellular morphology, multiplication capacity, MSC surface marking expression capacity, differentiative capacity and the like of the cell can be maintained.

The invention relates to the field of dairy products, and in particular provides a liquid dairy product containing a heat-sensitive substance and a preparation method thereof. The method for preparingthe dairy product containing the heat-sensitive substance comprises the following steps: 1) removing impurities from raw milk by using a milk purification process, and performing standardization on the raw milk; 2) performing pasteurization: sterilizing the standardized raw material at a temperature of between 70 and 90 DEG C for 5 to 15 seconds, and cooling the raw material to a temperature of 8DEG C; 3) proportioning materials: mixing the pasteurized milk with an auxiliary material, and homogenizing the mixture at a pressure of between 180 and 259 bars and at a temperature of between 40 and 75 DEG C; 4) performing aseptic processing on the heat-sensitive material; 5) performing ultrahigh temperature sterilization on the dairy products after the homogenization at a temperature of between 121 and 150 DEG C for 4 to 15 seconds; 6) adding the heat-sensitive substance at normal temperature on line sterilely at the later stage of the ultrahigh temperature sterilization; and 7) performingsubpackage. The products developed by using the method have diversified functionalities and can satisfy nutritional needs of different people.

The invention discloses a production process of fruit tea, comprising the steps of 1) selecting fruits (such as orange, tangerine, and lemon) uniform in size, washing the surface of the fruits with clear water, and sterilizing; 2) after the fruits are washed, making a cut in each fruit to obtain fruit skin covers, removing pulp in the fruits, dividing the fruits into cover and skin portions, washing the covers and the skin with clear water, sterilizing, removing residual juice, and naturally airing the skin; 3) placing aseptically treated heat conductors in the skin, sun-curing naturally in the sunlight, and after heat from sunlight is conducted to the heat conductors, allowing the heat conductors to naturally sun-cure the inside of the skin until the skin hardens and dries; 4) removing the heat conductors from the sun-cured skin, filling the skin evenly with tea, and closing the covers to obtain a finished product; 5) preserving the finished product in the environment with natural ventilation, zero peculiar odor, shadiness and dryness and coolness.

The present invention provides an apparatus for manufacturing sterilized water, spraying apparatus thereof and capsule containing salt using therein, more particularly, comprises a container having a water receiver for accommodating water; at least one negative electrode having at least one negative electrode projection formed thereon in the water receiver; at least one positive electrode having at least one positive electrode projection formed thereon arranged to face the negative electrode projection in the water receiver; and a power supply for supplying electric current to the negative electrode and the positive electrode, thereby promptly manufacturing a large amount of sterilized water within a short time, and thus, enabling users to use for disinfecting and sterilization the fresh sterilized water immediately after directly manufacturing the sterilized water without having aseptic to injured area or the inside of a nose for rhinitis' patients.

An apparatus for aseptic processing of a viscous food product includes a product tank configured to hold the viscous food product. A first pump is configured to pump the viscous food product from the product tank through a heat exchanger at a given rate. A cleaning station including a cleaning pump configured to pump a flushing fluid through the product tank. A second pump is configured to pump a flushing fluid through the heat exchanger at the same rate as the first pump, while the cleaning pump is pumping the flushing fluid through the product tank and first pump.

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.