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Method for cultivating gold-leaf sedge

A technology of golden leaf moss grass and culture medium, which is applied in the field of tissue culture of herbs, can solve the problems of slow ramets, few introductions, limited supply of seedlings, etc., and achieves the effects of increasing reproduction speed, increasing yield, and increasing survival rate.

Inactive Publication Date: 2013-03-06
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the small number of imported varieties introduced from abroad and the slow division, it is difficult to meet a large number of market demands, and the supply of seedlings is greatly limited
Through the tissue culture technique, the propagation speed and the uniformity of the seedlings are greatly improved, and the report of Carex aureus that better maintains the original female parent traits has not yet been seen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for cultivating Carex golden leaf, the method comprising the following steps:

[0029] (1) Sterile pre-treatment of small buds of Carex aureus

[0030] Pick the small buds of Carex aureus that grow robustly, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 30s, soak them in 1wt% mercuric chloride solution for 15mins, wash them with sterile water for 5-6 After the second time, dry the surface water with sterile filter paper, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA3.0mg / L+NAA0.3mg / L;

[0031] (2) Differentiation and proliferation of buds

[0032] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to swell, and yellow-green protrusions appeared, and after 2 weeks, obvious budlet meristems could be seen. Put the tissue into the proliferation medium MS+6-BA1.0mg / L+NAA0.1mg / L for culture, there...

Embodiment 2

[0040] A method for cultivating Carex golden leaf, the method comprising the following steps:

[0041] (1) Sterile pre-treatment of small buds of Carex aureus

[0042] Pick the small buds of Carex aureus that grow robustly, wash them with tap water for 2 hours, place them on a clean bench, soak them in 75wt% ethanol for 20s, soak them in 1wt‰ mercury chloride solution for 10mins, and wash them with sterile water for 5-6 After the second time, blot the surface moisture with sterile filter paper, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA1.0mg / L+NAA0.1mg / L;

[0043] (2) Differentiation and proliferation of buds

[0044] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to swell, and yellow-green protrusions appeared, and after 2 weeks, obvious budlet meristems could be seen. Put the tissue into the proliferation medium MS+6-BA2.0mg / L+NAA0.2mg / L for culture, there ar...

Embodiment 3

[0052] A method for cultivating Carex golden leaf, the method comprising the following steps:

[0053] (1) Sterile pre-treatment of small buds of Carex aureus

[0054] Pick the small buds of Carex aureus that grow robustly, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 40s, soak them in 1wt‰ mercury chloride solution for 20mins, wash them with sterile water for 5-6 After the second time, blot the surface moisture with sterile filter paper, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA5.0mg / L+NAA0.5mg / L;

[0055] (2) Differentiation and proliferation of buds

[0056] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to swell, and yellow-green protrusions appeared, and after 2 weeks, obvious budlet meristems could be seen. After one month of cultivation, the budlet meristems were cut off Put the tissue into th...

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PUM

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Abstract

The invention relates to a method for cultivating gold-leaf sedge. The method comprises the following steps of: picking a budlet of gold-leaf sedge; and performing sterile pretreatment, differentiation and multiplication of the budlet, adventitious bud acclimatizing culturing, rooting culturing, seedling adaptation and transplanting to obtain a gold-leaf sedge plant. Compared with the prior art, the method has the advantages that: by adopting a tissue culturing technology, the reproduction speed and the regularity of nursery stock can be greatly increased, the original maternal character is better kept, and the survival rate can be increased, so that the yield is increased and the transplanting survival rate is up to 95 percent.

Description

technical field [0001] The invention relates to a method for tissue culture of herbaceous plants, in particular to a method for cultivating Carex aureus. Background technique [0002] Carex aureus is a perennial evergreen herb with a plant height of 20 cm. The leaves have golden stripes, and the leaf clusters gushes out like a fountain. It is mainly for leaf viewing, with spikes, and the flowering period is April-May. In the garden, it is planted in clusters or arranged in flower borders or courtyards, and it can also be used as a large-area shade-tolerant ground cover. This species is semi-shade tolerant, has no strict requirements on the soil, and likes a humid environment with good drainage. At present, it is mostly used as foliage plants in flower beds or flower borders in gardens, and it can also be planted in pots for viewing. It is a good flower mirror and garden material. Due to the small number of imported varieties and the slow division of plants, it is difficult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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