A kind of breeding method of new banana variety
A cultivating method and a technology for new varieties are applied in the field of tissue culture of herb plants to achieve the effects of increasing survival rate, increasing yield and increasing reproduction speed
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Embodiment 1
[0026] A method for cultivating a new banana variety, the method comprising the following steps:
[0027] (1) Aseptic treatment
[0028] Dig out the small buds at the base of the banana, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 30s, soak them in 1wt‰ mercury solution for 15mins, wash them with sterile water for 5-6 times, and use them Blot the surface moisture with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA2.0mg / L+NAA0.2mg / L;
[0029] (2) Differentiation and proliferation of buds
[0030] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA1.0mg / L+NAA0.1mg / L was cultured, a...
Embodiment 2
[0038] A method for cultivating a new banana variety, the method comprising the following steps:
[0039] (1) Aseptic treatment
[0040] Dig out the small buds at the base of the banana, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 20s, soak them in 1wt‰ mercury chloride solution for 10mins, rinse them with sterile water for 5-6 times, and use them. Blot the surface moisture with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA1.0mg / L+NAA0.1mg / L;
[0041] (2) Differentiation and proliferation of buds
[0042] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA2.0mg / L+NAA0.2mg / L was ...
Embodiment 3
[0050] A method for cultivating a new banana variety, the method comprising the following steps:
[0051] (1) Aseptic treatment
[0052] Dig out the small buds at the base of the banana, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 40s, soak them in 1wt‰ mercury chloride solution for 20mins, rinse them with sterile water for 5-6 times, and use them. Blot the surface moisture with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA3.0mg / L+NAA0.3mg / L;
[0053] (2) Differentiation and proliferation of buds
[0054]Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA3.0mg / L+NAA0.3mg / L was c...
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