Amomum tsao-ko tissue culture and seedling raising method

Abstract

The invention belongs to the field of tissue culture of medicinal plants, and discloses an amomum tsao-ko tissue culture and seedling raising method. The amomum tsao-ko tissue culture and seedling raising method comprises the following steps of: selecting an explant: selecting the young stem segment at the base of an amomum tsao-ko stem; disinfecting the explant; performing primary induction culture; performing secondary enrichment culture; and performing rooting culture. According to the amomum tsao-ko tissue culture and seedling raising method disclosed by the invention, the adopted explantis the young stem segment at the base of the stem; compared with young shoots near the soil surface adopted at the beginning of an experiment, the contamination rate is decreased to 12% at the young stem segment from 95% at the young shoots near the soil surface; but, the inductivity of the young stem segment at the base of the stem is 80%, and slightly lower than the inductivity, namely, 84%, atthe young shoots near the soil surface.

Description

technical field [0001] The invention belongs to the field of tissue culture of medicinal plants, and in particular relates to a method for cultivating seedlings of Tsaoguo group. Background technique [0002] Caoguo (Amomumtsao-koCrivost et Lemaire) is a perennial herb of the genus Cardamom in the Zingiberaceae family. It grows in tropical and subtropical shaded and humid forests at an altitude of 1100-1800 meters, and is mainly distributed in Yunnan, Guangxi, Guizhou and other places. Its dried ripe fruit is used as medicine. It has the effects of drying dampness and warming the middle, eliminating phlegm and intercepting malaria. At the same time, Tsaoguo belongs to medicinal and edible medicinal materials, which are widely used as flavoring raw materials for food in the folk, and its whole plant (generally the fruit is the most) contains 1, 8-cineole, trans-2-decenal , Citral-b and other aromatic substances are often used to extract volatile essential oils as flavors and...

AI Technical Summary

Problems solved by technology

At present, it is known from the results of the national germplasm resource survey that due to the use of seedlings for perennial seedlings, the formation of hybridization between the populations has led to a large degree of variation in Tsaoguo, which makes Tsaoguo various production areas or even in the same production area. There are various varieties, resulting in The quality and yield of Tsaoko are uneven, and in some areas, the division propagation method is used for propagation and experimental planting. Although the traits of the female parent can be maintained to the greatest extent, it is found that due to the long planting period during the planting process, it has seriously degraded. , at the same time, due to the accumulation of pests and diseases, it is very easy to cause a pandemic of pests and diseases

[0004] In recent years, although there are reports on the method of cultivating seedlings of Tsaoko group, there are relatively few studies. There are research reports on the use of Tsaoko embryos for tissue culture (Hu Yan, etc.), but the embryos used are obtained by Tsaoko seed culture , there is also the possibility of hybridization variation, and the operation process uses the embryo of Tsaoko as an explant to carry out tissue culture, cultivate Tsaoko aseptic seedlings, take the stem section of Tsaoko aseptic seedlings to induce adventitious buds, and then carry out rooting culture. The period of the cultivation process is long, and large-scale production will increase the cost. In the existing method, the stem tip of Tsaoguo is used for tissue culture (Xiao Hongdong, etc.), but the multiplication factor of the treatment method only reaches 2.34, and adventitious buds appear. At the same time, there are various variations, such as leaf chlorosis, whole plant yellowing and death, etc.

In addition, in the process of induction and proliferation culture, the medium produces browning phenomenon, which leads to the death of the culture tissue, which affects the growth and development of the culture, and even leads to the overall death of the culture.

Images