The invention belongs to the field of traditional Chinese medicinal materials, specifically relates to the technical field of medicinal
plant cultivation, and in particular to a method for cultivating
Bletilla striata seedlings using an intermittent immersion
bioreactor; the method for cultivating
Bletilla striata seedlings using an intermittent immersion
bioreactor, Including seed sterilization, seed
germination, multiplication and cultivation,
pseudobulb generation and cultivation and
pseudobulb expansion and cultivation steps, the method for rapidly cultivating
Bletilla striata seedlings using intermittent immersion bioreactors is carried out under aseptic conditions, and the multiplication and cultivation includes transition Proliferation culture and Proliferation and rooting culture. Through the above technical scheme, the proliferation culture process is divided into two processes of transitional proliferation culture and proliferation and rooting culture, and the transitional proliferation culture eliminates or shortens the long
adaptation period from
solid culture to intermittent immersion
bioreactor culture by means of shake flasks phenomenon, thereby rapidly obtaining a large amount of
bletilla striata seedlings for production, and then obtaining the method for the
pseudobulb seedlings through the cultivation of pseudobulb expansion.