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4results about How to "Improve multiplication efficiency" patented technology

Transplanting and planting method for efficient field propagation of detoxified original sugarcane seedlings

PendingCN119278831AHigh transplant survival rateImprove multiplication efficiencySugarcane cultivationSugar caneVirus
The invention belongs to the field of sugarcane planting, and particularly relates to a transplanting planting method for efficient field propagation of detoxified original sugarcane seedlings. According to the method disclosed by the invention, on the premise of ensuring the quality (stem diameter thickness and seed bud vitality) of the harvested virus-free seed stems, more effective lateral buds of the virus-free seed stems can be obtained in unit area, and the reduction of the large-field large-scale breeding cost of the sugarcane virus-free seedlings and the improvement of the overall propagation efficiency are promoted; the method has a positive promotion effect on popularization and planting of sugarcane virus-free seedlings in China and improvement of the coverage rate of improved varieties.
Transplanting and planting method for efficient field propagation of detoxified original sugarcane seedlings
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Owner:GUANGXI INSTITUTE OF TROPICAL AGRICULTURAL SCIENCES +1

Low serum msb1 suspension culture medium and chicken infectious anemia virus inactivated vaccine

ActiveCN122081234Amaintain growth activitymaintain structureViral antigen ingredientsAntiviralsBioreactorAmino acid
This invention discloses a low-serum MDCC-MSB1 (MSB1) suspension culture medium and an inactivated chicken infectious anemia virus (TIV) vaccine. The culture medium comprises amino acids, carbon source, additives, vitamins, inorganic salts, buffer salts, and water, and further contains newborn calf serum (NBCS). Using this medium, low-cost, high-density suspension culture of MSB1 cells can be achieved, significantly improving cell culture stability. After 30 consecutive passages at a 1% volume ratio of NBCS, the viability of MSB1 cells remains above 94%, with uniform cell morphology and a clumping rate of <3%, making it suitable for large-scale bioreactor production. Furthermore, when applied to the preparation of an inactivated chicken infectious anemia virus vaccine, it can comprehensively improve virus propagation efficiency, increasing virus production efficiency by more than 25%, with a virus titer of not less than 1.0 × 10⁻⁶. 8.0 TCID 50 / ml, ensuring stable quality in large-scale production.
Low serum msb1 suspension culture medium and chicken infectious anemia virus inactivated vaccine
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Owner:SUZHOU WOMEI BIOLOGY CO LTD

A food residue culture medium and a method for propagating lignocellulose-degrading bacteria and applications thereof

ActiveCN116024101Breduce pollutionInhibition of reproductive metabolismBiotechnologyXylanase
The present invention belongs to the technical field of composting. The food residue culture medium provided by the present invention comprises the following raw material components, measured by the volume of solvent water: 200-300 g / L of pomace, 100-150 g / L of bean dregs and 3-5 g / L of CaCO3; the pH value of the food residue culture medium is 6-7. The culture medium for expanding lignocellulose-degrading bacteria prepared from the food residue has low cost and is rich in sugars, proteins, fruit acids, vitamins and minerals. The food residues are fully utilized while solving the technical problems of high cost and low efficiency of conventional expansion methods of lignocellulose-degrading bacteria agents, and improving the xylanase activity, cellulase activity, laccase activity, manganese peroxidase activity and lignin peroxidase activity in the expanded product. The lignocellulose-degrading bacterial liquid obtained by expanding the food residue culture medium is applied to garden waste composting to improve the quality of compost.
A food residue culture medium and a method for propagating lignocellulose-degrading bacteria and applications thereof
A food residue culture medium and a method for propagating lignocellulose-degrading bacteria and applications thereof
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Owner:SHIJIAZHUANG INST OF AGRI MODERNIZATION CHINESE ACAD OF SCI

Feeding tray for automatic large-scale production of noctuid larvae

ActiveCN222302807UImprove multiplication efficiencylow costAnimal husbandryFisherySpodoptera
The utility model discloses a feeding tray for automatic scale production of noctuid larvae, which comprises a body with an independent unit chamber, the independent unit chamber is provided with a first upper opening, an accommodating body is arranged in the internal space of the independent unit chamber, the accommodating body is provided with an accommodating chamber for accommodating eggs of the noctuid larvae and protective substances, and the accommodating chamber is provided with a second upper opening. The containing body is provided with a second upper opening, the internal space of the independent unit cavity further comprises a feed space for containing feed, the feed space is communicated with the second upper opening, and the position of the second upper opening is lower than the position of the first upper opening in the vertical direction; during feeding, after the eggs in the accommodating chamber grow, the eggs can enter the feed space through the second upper opening. According to the feeding tray, feeding of eggs and larvae in multiple different growth stages can be completed, only one-time feeding material adding and subsequent film sealing are needed, the propagation expanding efficiency is greatly improved, the cost can be reduced, and the feeding period can be shortened.
Feeding tray for automatic large-scale production of noctuid larvae
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Owner:YUNNAN TOBACCO CO LTD KUNMING BRANCH +1