56results about How to "Improve multiplication efficiency" patented technology

The invention discloses a high-efficient large-scale propagation technology for mycorrhiza fungi inoculum, and belongs to the field of bio-fertilizer productions. The high-efficient large-scale propagation technology suitable for mycorrhiza fungi includes material selection, production process, quality control and product formation. Materials for the technology are easy to obtain, operation is easy and convenient and cost is low. Spore density of the propagated mycorrhiza fungi inoculum can reach more than fifty per cubic centimeter, and can even reach more than one hundred per cubic centimeter, and propagation efficiency is more than four times higher as compared with conventional propagation technology efficiency. The device adopted in the technology is high in flexibility, easy to be regulated in real time and small in occupation. Detection of product quality of the mycorrhiza fungi and large-scale production thereof are facilitated. An AMF (abuscular mycorrhizal fungi) propagation technology, high-efficient and prone to large-scale production, formed in the technology, is beneficial to application of the AMF in agricultural production, and new approaches for sustainable development for agriculture are provided.

The invention relates to the technical field of information storage, in particular to a DNA-based information storage method. A long sequence in vivo DNA information storage technique is provide. Themain goal is to construct a coding system with strong error correction mechanism based on LDPC and BCH codes, which can reduce the redundancy of primers and indexes by long sequence coding, and achieve a high actual carrying capacity (above 97%). The main goal is to construct a medium-length DNA sequence (above 1Kbp). Saccharomyces cerevisiae in vivo assembly system is used to assemble and store long sequences and preserve information, and the information can be replicated at low cost, high fidelity and high speed by model organisms such as Saccharomyces cerevisiae, Escherichia coli or Bacillus. At the same time, because of the existence of strong error correction system, the data in the bacterial cell can be reduced perfectly with low coverage (1-5 X) in the second generation and third generation sequencing.

The invention discloses a rosemary stem tip cutting propagation method, which comprises the following operation steps: 1) manufacturing cutting media, 2) cutting the rosemary stem tips, 3) inducing the rooting of cutting slips, 4) cutting the cutting slips, 5) managing the cutting slips before rooting, and 6) cultivating seedlings. According to the cutting propagation method for the rosemary disclosed by the invention, the stem tips of the rosemary are cutting slips, and every stem tip of the rosemary can become a seedling; compared with the efficiency of the existing seed propagation method,the efficiency of the rosemary stem tip cutting propagation method is increased by more than 30 times; and the cutting slips firstly root, and are then induced to sprout, the roots of the cutting slips are strong, the transplantation survival rate is high, and the production cost is reduced.

The invention discloses a high-density expanding propagation method of healthy sugarcane seedlings, which comprises the steps of (1) ridging and ditching, (2) high-density planting, (3) fine field management and (4) shearing and harvesting and stubble cane management. According to the method, in the processes of seed production and expanding propagation of the healthy sugarcane seedlings, a high-density expanding propagation technology is adopted for expanding propagation, so that the purposes of saving land resources, reducing reinfection rate of a pathogen and increasing the total number of buds in unit area are achieved, the intensive and fine management is realized, the expanding propagation efficiency is improved, and a positive promotion effect is played on accelerating the popularization of the healthy sugarcane seedlings.

The invention discloses a Japanese coccophagus expanding propagation method. The method includes the first step of host plant preparation, the second step of expanding propagation of an intermediate host, namely parasaissetia nigra, and the third step of expanding propagation and rejuvenation of Japanese coccophagus. The method is a simple and efficient intermediate host collection and inoculation method, the survival rate of the intermediate host, namely parasaissetia nigra, is increased, the utilization rate of host plants is increased to the largest extent, the parasitism rate of Japanese coccophagus on the intermediate host is increased, the number of pest populations is controlled, and the field prevention effect is ensured.

The invention relates to the technical field of crop cultivation, in particular to a cultivation method for potato breeder's seeds. The cultivation method includes the steps of rapid propagation of virus-free seedlings, seedling hardening, transplanting, seedling stage management, harvesting and storage. The link of strong seedling culture before transplanting potato tissue culture seedlings is omitted, the inoculum density is increased in the link of rapid propagation of the virus-free seedlings, the inoculum mode is changed, the use ratio of a culture medium is greatly increased, propagation efficiency is improved, power consumption cost and culture space are reduced, and the cost of link of rapid propagation of the potato tissue culture seedlings is reduced; density is reduced in the transplanting link, multi-plant cuttage is carried out, traditional strong seedling transplanting is changed to be weak seedling transplanting, top ends perish after medium weak seedlings are transplanted and survive, apical dominance is broken, plant branching is improved, the number of single plant branches is increased to 2-4 from 1-2, plant growth vigor is improved, and the breeder's seed yield is increased. Meanwhile, the fertilization mode and fertilizer type are improved, so that management cost is reduced, and the yield is increased.

The invention discloses a switchgrass cuttage vegetative propagation method which comprises the following steps: when the switchgrass grows to period with 4-6 knobs, removing the top and leaving 3-4 knobs below the top so as to break the apical dominance and prompt the growth of axillary buds on the knobs below; when lateral buds of 15-20 cm long grow from the axillary buds, cutting the lateral buds together with the parts which are 5 cm long above and below the knobs, so as to be the cuttings; trimming the cuttings and carrying out TDZ (Thidiazuron) rooting powder treatment, and cuttaging the cuttings into a culture medium to carry out rooting culture; and transplanting to a greenhouse or a land after the rooting is accomplished. According to the switchgrass cuttage vegetative propagation method disclosed by the invention, the vegetative propagation is tried on perennial switchgrass of the grass family by using the cuttaging method, and unexpected and good breeding effect is achieved; and the method has the characteristics of being simple and feasible, high in survival rate, unlikely to have variation and the like, and is particularly applicable to providing materials for switchgrass biotechnology study.

The invention belongs to the field of traditional Chinese medicinal materials and particularly relates to a method for culturing a bletilla striata seedling by virtue of an intermittent immersion type bioreactor. The method sequentially comprises the steps the following steps: seed sterilization, seed germination, enrichment culture, pseudobulb generation culture and pseudobulb expansion culture. The method for culturing the bletilla striata seedling by virtue of the intermittent immersion type bioreactor is carried out under aseptic conditions; the enrichment culture step comprises a transition enrichment culture step and a multiplication and rooting culture step. By virtue of the technical scheme, the enrichment culture process is divided into the transition enrichment culture process and the multiplication and rooting culture process, and in the transition enrichment culture process, a long adaptation phenomenon occurring from a solid culture process to an intermittent immersion type bioreactor culture process is eliminated or shortened by virtue of a bottle shaking manner, so that lots of bletilla striata seedlings for production are rapidly obtained and then are subjected to pseudobulb expansion culture to produce seedlings with pseudobulbs.

The invention discloses a propagation method for healthy sugarcane seedlings in a temporary planting nursery. The method comprises the following steps: (1) cultivating sugarcane virus-free seed stems in the temporary planting nursery; (2) performing successive multiplication culture on the sugarcane virus-free seed stems in the temporary planting nursery; (3) sprouting and raising seedlings in nutritious cups; (4) propagating or planting the seedlings in the nutritious cups; and the like. According to the method, a temporary planting nursery propagation technology is adopted in a healthy sugarcane seedling seed production and propagation process to achieve the purpose of saving the land resource; meanwhile, the reinfection rates of both ratoon stunting disease and mosaic disease of each stage of seedling field are lower than those of the conventional method. By applying the method, intensive and standardized management can be realized, and the propagation efficiency is improved; the method has a positive promotion effect on the generalization and promotion of the healthy sugarcane seedlings.

The invention provides a cutting propagation method of Miscanthus giganteus, which comprises the steps of: (1) cutting treatment of a mature stem of the Miscanthus giganteus: stripping the stem from a leaf sheath and cutting into segments of 10-12cm as shoots for cutting, and obliquely cutting into a substrate after induced hormone treatment of axillary bud; (2) germination and cultivation of the axillary bud: controlling the temperature of the substrate at high temperature through warming geothermal lines for inducing the axillary bud to germinate and come out of the soil, and cultivating the growth of the bud by controlling the day temperature / night temperature at 20-30 DEG C / 15-20 DEG C respectively; and (3) rooting cultivation of the bud: transplanting the bud to a nutrition pot after the bud grows to 15-20 cm or more, paving the geothermal lines to control the temperature of the substrate and promoting the rooting of the bud. The method has the advantages of high propagation efficiency, robust cuttage root system and high transplanting survival rate, and is a mature technology suitable for commercial propagation of the Miscanthus giganteus.

The invention relates to a production method and a device of a three-phase biological bacterium organic fertilizer, which is characterized in that biogas fermentation, an anaerobic bacteria species propagation and fermentation integration continuous and rapid fermenting device and a three-phase rapid fermentation technology are used for processing city industrial organic waste materials, crops straws and human and animal night soil in a harmlessness manner to produce the high efficiency biological bacterium organic fertilizer. According to the invention, in a first phase, the animal dung with high carbon-nitrogen ratio is used for propagation and fermentation to produce secondary liquid strain; the secondary liquid strain with high carbon-nitrogen ratio is used as a main nitrogen source for high temperature aerobic fermentation in a second phase, and a third phase anaerobic fermentation is carried out after the second phase high temperature aerobic fermentation. The characteristics of high water content, high nitrogen and low carbon of human and animal night soils are used for rapidly decomposing by employing biogas fermentation to produce a lot of humic acid, amino acid and available nitrogen, rapidly available phosphorus, and rapidly available potassium. A biogas broth is used for propagation and fermentation of biological bacterium, the biological bacterium propagation technology and production equipment are simplified, a lot of fermentation raw materials can be saved, biological bacterium propagation efficiency is accelerated, and beneficial biological bacterium survival rate can be increased.

The invention discloses an efficient expanding propagation method for dahlia toxin-free seedlings. The method includes the following steps of 1, pretreatment of dahlia toxin-free seedlings, 2, induction of adventitious buds, 3, cutting of adventitious buds for mulitiplication, 4, hardening-seedling, 5, water planting and rooting, 6, water planting of strong seedlings and 7 transplanting. The efficient expanding propagation method solves the problems that dahlia toxin-free seedlings are low in efficiency in the expanding propagation process and prone to secondary pollution. A great number of excellent dahlia toxin-free seedlings can be cultured in an expanding propagation mode in a short period of time, the problem of variety degeneration brought by dahlia division propagation and seed propagation can be avoided, and the method is easy to operate and has higher practical value in production, and industrialized popularization is achieved easily.

The invention relates to a salamandrina breeding method and in particular relates to an artificial forcing breeding method for batrachuperus pinchonii. The method is technically characterized by comprising the following steps: building a culture pond; feeding breeding batrachuperus pinchonii; incubating the breeding batrachuperus pinchonii; managing the young batrachuperus pinchonii, optimizing the growth environment of the batrachuperus pinchonii, and performing scientific management. The birth and survival rate is improved at each link of the whole batrachuperus pinchonii growth process, the growing period is shortened, and the weight of an individual batrachuperus pinchonii is increased, so that the quality of the batrachuperus pinchonii is well guaranteed. According to the forcing breeding method disclosed by the invention, the fertilization rate of the batrachuperus pinchonii is increased by 30% compared with that under natural conditions, the survival rate of the batrachuperus pinchonii is improved by 50% compared with that under natural conditions, the growing period of the batrachuperus pinchonii is shortened by 150-200 days, and the weight of the individual batrachuperus pinchonii is increased by 26% year on year.

The invention relates to the field of computers, in particular to an SM2 elliptic curve public key cryptosystem optimization method, which adopts a comb algorithm of two pre-stored tables to optimizefixed point multiple point operation in an SM2 digital signature algorithm. For fixed point multi-point operation in the SM2 public key encryption algorithm, a comb algorithm of two pre-stored tablesis adopted for optimization; for unfixed-point multi-point operation on the curve, a multi-point Montgomery method is adopted for optimization; for unfixed-point multi-point operation on an E (GFp) curve, a projective coordinate algorithm is adopted for optimization. According to the method, the operation efficiency is improved through an optimization algorithm, and the digital signature, key exchange and data encryption performance of the method are optimized.

The invention belongs to the technical field of plant cell engineering. A culture method of holboellia fargesii reaub germchits sequentially comprises the following steps: pre-treating, obtaining sterile explants, inducing calluses, carrying out induction and multiplication on cluster buds, carrying out rooting culture and transplanting. With the adoption of the technical scheme for producing the holboellia fargesii reaub germchits, the culture method has the beneficial effects that (1) the multiplication rate is high, the biological yield is great and the culture period is short; (2) the quality of tissue culture seedlings is good, the germchits are strong and the culture survival rate is high; the holboellia fargesii reaub germchits are cultured by using the method comprising the steps of pre-treating, obtaining the sterile explants, inducing the calluses, carrying out induction and multiplication on the cluster buds, carrying out rooting culture and transplanting; compared with other culture manners, the culture method has high multiplication efficiency, short culture time and convenience for transplanting; the germchits are strong, the culture survival rate is high and the cost is low; and therefore, the culture method is suitable for large-scale culture and production on the holboellia fargesii reaub germchits.

The invention discloses a system for expanding propagation of arbuscular mycorrhizal fungi with alfalfa as a host. The system comprises a plurality of fixed stand columns, a plurality of fungus expanding propagation devices are arranged on each fixed stand column at intervals in the height direction of the fixed stand column, each fungus expanding propagation device comprises an inner planting cavity and an outer planting cavity, and a fungus expanding propagation cavity is formed between each inner planting cavity and the corresponding outer planting cavity; and the two planting cavities communicate with the corresponding fungus expanding propagation cavity through a plurality of fungus propagation diffusion channels, so that fibrous roots and arbuscular mycorrhizal fungi of the alfalfa are propagated into the fungus expanding propagation cavities. According to the system, arbuscular mycorrhizal fungi and some fibrous roots can be introduced into fungus matrix soil in the fungus expanding propagation cavities through the fungus propagation diffusion channels, hyphae, spores and the like are attached into the fungus matrix soil, in this way, after the fungus matrix soil is collected, an arbuscular mycorrhizal fungus inoculant is prepared, then the fungus expanding propagation cavities are filled with new fungus matrix soil again, the growth of alfalfa root systems and the growth and propagation of fungi are not influenced, and the expanding propagation efficiency of arbuscular mycorrhizal fungi is improved while continuous production is realized.

The invention discloses a regeneration and propagation method for optimized fresh ginger virus-free seedlings and development of the industrialized flow of the optimized fresh ginger virus-free seedlings. The regeneration and propagation method is characterized in that an optimal explant type, disinfection method, cytokinin, and induction medium formula, seedling hardening condition and cultivation condition are determined. It requires 3.5-4.5 months for the optimized fresh ginger virus-free seedlings from explants to TC0 rooting seedlings, and it takes about 1.5 months for each time of subsequent subculture; the bacteria pollution rate in the whole process is less than 5%; the budding percentage of TC0 can reach up to 800%; the differentiation efficiency can reach up to 300% for each subsequent subculture; the rooting rate can reach 100%; the survival rate of transplanting of tissue cultured seedlings can reach 80% or more, and the rooting seedlings can be applied to industrializationas a ginger seed after being propagated in a field for 2-3 generations; and the virus-free seedlings in the method have high propagation efficiency, a good condition and a short cycle.

The invention relates to a Lycium hard-branch cuttage seedling raising method through hotbed root promotion and hormone enhancement. The method is characterized by comprising the steps: collecting cuttings, treating base parts, performing hotbed root promotion, applying a plant hormone brassinolide to cutting trenches, putting seedling in the cutting trenches, performing field management and the like. The seedling rate of Lycium hard-branch cuttage reaches 75%-88%, the expanding propagation efficiency of improved varieties of Lycium is improved, and the cost of seedling raising is reduced.

The invention provides a tissue culture rapid propagation technology for slash pine seedlings, which belongs to the field of plant nutrition and cultivation technology, and comprises the following steps: pretreating a mother plant, taking explants from the mother plant, and explanting the explants Disinfection of implants; induction of tender stems; multiplication of clustered lateral buds; rejuvenation culture of rootless buds; rooting culture; domestication culture; transplantation of tissue cultured seedlings. The invention provides a tissue culture rapid propagation technology for slash pine seedlings, through inducing and culturing the tender stems of slash pine, obtaining germinated seedlings, and obtaining a large number of rootless buds through the method of proliferating clustered buds. Then the rootless sprouts are rejuvenated by tissue culture, induced to take root, and can be transplanted out of the bottle after natural acclimation in the greenhouse. According to the technology, the progeny seedlings bred have stable genetics, comprehensive character expression, and excellent ornamental properties. Moreover, the technical system has low production cost, is environmentally friendly and does not pollute the environment, and has a fast mass production speed, fully realizing the technical requirements for large-scale mass production.

The invention discloses a method for multiplying the wasps of A. japonica, which comprises the following steps: (1) treatment of host plants (2) expansion and propagation of the intermediate host A. Complex and rejuvenated. The present invention has a simple and efficient method for collecting and inoculating intermediate hosts, which improves the survival rate of the intermediate host, the parasitic wax scale, maximizes the utilization rate of the host plant, increases the parasitism rate of the Japanese scale-eating wasp to the intermediate host, and realizes In order to control the number of pest populations and ensure the effectiveness of field control.

The invention belongs to the field of traditional Chinese medicinal materials, specifically relates to the technical field of medicinal plant cultivation, and in particular to a method for cultivating Bletilla striata seedlings using an intermittent immersion bioreactor; the method for cultivating Bletilla striata seedlings using an intermittent immersion bioreactor, Including seed sterilization, seed germination, multiplication and cultivation, pseudobulb generation and cultivation and pseudobulb expansion and cultivation steps, the method for rapidly cultivating Bletilla striata seedlings using intermittent immersion bioreactors is carried out under aseptic conditions, and the multiplication and cultivation includes transition Proliferation culture and Proliferation and rooting culture. Through the above technical scheme, the proliferation culture process is divided into two processes of transitional proliferation culture and proliferation and rooting culture, and the transitional proliferation culture eliminates or shortens the long adaptation period from solid culture to intermittent immersion bioreactor culture by means of shake flasks phenomenon, thereby rapidly obtaining a large amount of bletilla striata seedlings for production, and then obtaining the method for the pseudobulb seedlings through the cultivation of pseudobulb expansion.

The invention discloses a culture method for alexandtium minutum capable of generating gonyautoxin. Conventional culture method and conditions are adopted for culturing alexandtium minutum, and a marine red yeast extract is added into a culture medium in the initial culture stage of alexandtium minutum. A preparation method for the marine red yeast extract comprises the following steps: taking drymarine red yeast powder, and then adding distilled water, (S)-(+)-3-(1-naphthoxy)-1-phenyl-1-propyl alcohol and citric acid, preparing into a suspension and ultrasonically treating; properly regulating pH and temperature of the solution, adding papain and performing enzymolysis; centrifuging, taking supernate and freeze-drying, thereby acquiring the marine red yeast extract. The invention has thebeneficial effects as follows: the invention provides a simple and efficient culture method for alexandtium minutum capable of generating gonyautoxin, the culture process is unlikely to result in pollution, and the alexandtium minutum is high in growth rate and high in toxin yield.

The invention relates to chrysopa formosa packaging and storing methods. According to the growth stage of chrysopa formosa, the proper packaging method is selected, three-dimensional paper card type packaging is adopted for chrysopa formosa eggs, bottle type packaging is adopted for chrysopa formosa larvae, three-dimensional paper card or bottle type packaging is adopted for chrysopa formosa cocoons, and cassette packaging is adopted for chrysopa formosa adults. The storing method comprises short term refrigeration storing and / or long term diapause storing. Accordingly, simple, rapid and efficient packaging and effective storing of all insect state products of the chrysopa formosa are achieved, the product shelf life is significantly prolonged, the product storage quantity is increased, the packaging, transportation and storing cost is lowered, standardized perennial large-scale production of the chrysopa formosa is easily achieved, the methods can be used for controlling multiple agriculture and forestry pests such as aphides, whitefly, scale insects, pest mites, lepidoptera eggs and young larvae, and biological control needs of facility vegetables, fields, orchards, flowers and the like are met.