The invention relates to a microbial strain
screening method based on self-
distillation deep learning, and belongs to the crossing field of
synthetic biology and
artificial intelligence. According to the method, lightweight self-
distillation network modeling is carried out on
DNA sequences of microbial strains, the microbial strains with
high protein expression quantity are efficiently screened, and a
screening tool is provided for cost and
mass balanced
genotype-
phenotype design. The method comprises the following steps: carrying out One-hot two-dimensional matrix coding and high-dimensional
feature extraction on
a DNA sequence of a microbial strain; constructing a super
network topology architecture by using the encoded
DNA sequence, and performing architecture weight optimization and sampling according to the distillability of the current dominant architecture; in order to simplify a super
network structure and reduce calculation complexity, a filtering module is introduced for
global optimization and candidate operation filtering, so that a potential architecture space is compressed; finally, strategy knowledge
distillation is carried out on the compressed
network architecture and the
network architecture of the
previous generation, so that knowledge in the architecture of the
previous generation is migrated to the current architecture, and the prediction precision and efficiency of the
protein expression quantity of the microbial strain are improved. According to the technical scheme, the problems of
protein expression quantity prediction and microbial strain
DNA prototype design and screening can be solved by effectively utilizing a lightweight
deep learning model, and the screening cost is effectively reduced compared with a traditional
genetic engineering method.