Cell culture device convenient to add liquid

Abstract

The utility model discloses a cell culture device convenient to add liquid, which belongs to the technical field of cell culture and comprises a culture dish, a placing plate is arranged in the culture dish, a plurality of culture holes are formed in the placing plate, a cover plate is arranged at the top of the culture dish, a plurality of first through holes are formed in the cover plate, and a plurality of second through holes are formed in the first through holes. And the cover plate is rotationally connected with a rotating plate. According to the cell culture device convenient to add the liquid, by arranging a rotating disc, a second through hole, a first through hole, a rotating plate and a screw rod, a worker enables an injector to penetrate through the first through hole and the second through hole and adds a nutrient solution into a culture hole, after adding is completed, a nut is rotated to drive the rotating plate and the rotating disc to rotate, and when the second through hole is aligned with the solid part of a cover plate, the rotating plate is rotated; according to the device, when a nutrient solution needs to be added into one culture hole, the cover plate does not need to be wholly opened, so that the cell pollution probability is reduced, and the cell culture success rate is increased.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, in particular to a cell culture device which is convenient for adding liquid. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. Cell culture devices are required in the cell culture process.

[0003] A cell culture device is generally a cell culture dish. Existing culture dishes generally have multiple culture wells inside. When nutrient solution needs to be added to one of the culture wells, the entire cover needs to be opened, which makes the numerous culture wells inside the culture dish exposed to the air, making them very susceptible to contamination, resulting in a reduced success rate of cell culture. Utility Model Content

[0004] In order to overcome the above defects, the utility model provides a cell culture device that is easy to add liquid, which solves the problem that when adding nutrient solution to one of the culture wells of the existing cell culture device, the cover needs to be opened completely, resulting in a reduced success rate of cell culture.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture device that is easy to add liquid, comprising a culture dish, a placement plate is arranged inside the culture dish, a plurality of culture holes are opened on the placement plate, a cover plate is arranged on the top of the culture dish, a plurality of first through holes are opened on the cover plate, a rotating plate is rotatably connected to the cover plate, a screw is threadedly connected to the rotating plate, the rotating plate is fixedly connected to a rotating disk through the cover plate, a second through hole is opened on the rotating disk, and the diameter of the second through hole is equal to that of the first through hole.

[0006] As a further solution of the utility model: a nut is fixedly connected to one end of the screw rod, and four suction cups are fixedly connected to the bottom of the culture dish.

[0007] As a further solution of the utility model: two fixing blocks are fixedly connected to the cover plate, and a limiting rod is slidably connected to the fixing block.

[0008] As a further solution of the utility model: a tension spring is sleeved on the limit rod, and a round cap is fixedly connected to one end of the limit rod.

[0009] As a further solution of the utility model: two positioning holes are provided on the culture dish, and two limiting holes are provided on the culture dish.

[0010] As a further solution of the utility model: two positioning rods are fixedly connected inside the cover plate, and the positioning rods correspond to the positioning holes.

[0011] Compared with the prior art, the beneficial effects of the utility model are:

[0012] 1. The cell culture device that is easy to add liquid is provided with a turntable, a second through hole, a first through hole, a rotating plate and a screw. When nutrient solution needs to be added to the culture well, the nut is rotated to drive the rotating plate and the turntable to rotate around the cover plate, so that the second through hole is aligned with the culture well to which the nutrient solution needs to be added, and the second through hole is also aligned with the first through hole. At this time, the nut is turned to drive the screw to rotate. When the screw contacts the cover plate, the rotating plate and the turntable are fixed. At this time, the staff passes the syringe through the first through hole and the second through hole and adds nutrient solution to the culture well. After the addition is completed, the nut is turned to drive the rotating plate and the turntable to rotate. When the second through hole is aligned with the solid part of the cover plate, the nut is turned to fix the rotating plate and the turntable, and the culture dish can be sealed. When the device needs to add nutrient solution to one of the culture wells, the cover plate does not need to be opened entirely, thereby reducing the probability of cell contamination and improving the success rate of cell culture.

[0013] 2. The cell culture device that is easy to add liquid is provided with a limit rod, a tension spring, a round cap and a limit hole. When the cover plate needs to be fixed to the culture dish, the two round caps are pulled to drive the two limit rods to move away from each other, and the positioning rod is inserted into the positioning hole. Under the positioning of the positioning rod and the positioning hole, the limit hole and the limit rod are aligned. At this time, the round cap is loosened, the tension spring pulls the two round caps and the limit rod closer to each other, and the limit rod is inserted into the limit hole, so that the cover plate can be fixed on the culture dish. By fixing the cover plate on the culture dish, the cover plate can be prevented from being separated from the culture dish, causing the culture hole to be exposed to the air, thereby improving the stability of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0015] Figure 2 This is a schematic diagram of the structure of the culture dish and the cover plate being separated according to the utility model;

[0016] Figure 3 For this utility model Figure 2 The enlarged structural diagram at A in the middle;

[0017] Figure 4 It is a structural schematic diagram of the cross section of the cover plate of the utility model;

[0018] In the figure: 1. culture dish; 2. cover plate; 3. first through hole; 4. nut; 5. round cap; 6. suction cup; 7. screw; 8. rotating plate; 9. placement plate; 10. culture hole; 11. positioning hole; 12. limiting hole; 13. fixing block; 14. limiting rod; 15. tension spring; 16. positioning rod; 17. turntable; 18. second through hole. DETAILED DESCRIPTION

[0019] The technical solution of this patent is further described in detail below in conjunction with specific implementation methods.

[0020] like Figure 1-4 As shown, the utility model provides a technical solution: a cell culture device that is easy to add liquid, including a culture dish 1, a placement plate 9 is arranged inside the culture dish 1, two positioning holes 11 are opened on the culture dish 1, and two limiting holes 12 are opened on the culture dish 1.

[0021] A plurality of culture holes 10 are provided on the placement plate 9 , and a cover plate 2 is provided on the top of the culture dish 1 , to which two fixing blocks 13 are fixedly connected, and to which a limiting rod 14 is slidably connected.

[0022] A plurality of first through holes 3 are provided on the cover plate 2, a rotating plate 8 is rotatably connected to the cover plate 2, a screw rod 7 is threadedly connected to the rotating plate 8, and because the screw rod 7 is provided, the turntable 17 is fixed by locking the screw rod 7 to prevent the second through hole 18 from shifting position, thereby facilitating the staff to add nutrient solution, a nut 4 is fixedly connected to one end of the screw rod 7, and because the nut 4 is provided with a plurality of grooves, friction can be increased, and it is convenient for the staff to rotate the nut 4, thereby improving the convenience of operation, and four suction cups 6 are fixedly connected to the bottom of the culture dish 1, and because the suction cup 6 is provided, the culture dish 1 is tightly adsorbed inside the incubator through the suction cup 6 to prevent multiple culture dishes 1 from colliding with each other when a person collides with the incubator, thereby protecting the culture dish 1.

[0023] The rotating plate 8 passes through the cover plate 2 and is fixedly connected with a rotating disk 17. A second through hole 18 is opened on the rotating disk 17. The diameter of the second through hole 18 is equal to that of the first through hole 3. A tension spring 15 is sleeved on the limiting rod 14. A round cap 5 is fixedly connected to one end of the limiting rod 14. Two positioning rods 16 are fixedly connected inside the cover plate 2. The positioning rods 16 correspond to the positioning holes 11. Since the positioning rods 16 are provided, the positioning rods 16 cooperate with the positioning holes 11 to position the cover plate 2, so that the limiting holes 12 are aligned with the limiting rods 14. There is no need to adjust the position of the cover plate 2 multiple times, thereby facilitating the fixing of the cover plate 2 to the culture dish 1.

[0024] The working principle of the utility model is:

[0025] When it is necessary to add nutrient solution to the culture well 10, the nut 4 is turned to drive the rotating plate 8 and the rotating disk 17 to rotate around the cover plate 2, so that the second through hole 18 is aligned with the culture well 10 to which the nutrient solution needs to be added, and the second through hole 18 is also aligned with the first through hole 3. At this time, the nut 4 is turned to drive the screw 7 to rotate. When the screw 7 contacts the cover plate 2, the rotating plate 8 and the rotating disk 17 are fixed. At this time, the staff passes the syringe through the first through hole 3 and the second through hole 18 and adds nutrient solution to the culture well 10. After the addition is completed, the nut 4 is turned to drive the rotating plate 8 and the rotating disk 17 to rotate. When the second through hole 18 is aligned with the physical part of the cover plate 2, the nut 4 is turned to fix the rotating plate 8 and the rotating disk 17, and the culture dish 1 can be sealed;

[0026] When it is necessary to fix the cover 2 to the culture dish 1, pull the two round caps 5 to drive the two limit rods 14 away from each other, insert the positioning rod 16 into the positioning hole 11, and under the positioning of the positioning rod 16 and the positioning hole 11, the limit hole 12 is aligned with the limit rod 14. At this time, release the round cap 5, the tension spring 15 pulls the two round caps 5 and the limit rod 14 closer to each other, and inserts the limit rod 14 into the limit hole 12, so that the cover 2 can be fixed on the culture dish 1. When removing the cover 2, you only need to repeat the above operation in reverse.

[0027] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0028] The above describes in detail the preferred implementation of this patent, but this patent is not limited to the above implementation. Various changes can be made within the knowledge scope of ordinary technicians in this field without departing from the purpose of this patent.

Claims

1. A cell culture device that is convenient for adding liquid, comprising a culture dish (1), characterized in that: A placement plate (9) is arranged inside the culture dish (1), and a plurality of culture holes (10) are provided on the placement plate (9). A cover plate (2) is arranged on the top of the culture dish (1), and a plurality of first through holes (3) are provided on the cover plate (2). A rotating plate (8) is rotatably connected to the cover plate (2), and a screw (7) is threadedly connected to the rotating plate (8). The rotating plate (8) passes through the cover plate (2) and is fixedly connected to a rotating disk (17), and a second through hole (18) is provided on the rotating disk (17), and the diameter of the second through hole (18) is equal to that of the first through hole (3).

2. A cell culture device that is easy to add liquid according to claim 1, characterized in that: A nut (4) is fixedly connected to one end of the screw rod (7), and four suction cups (6) are fixedly connected to the bottom of the culture dish (1).

3. A cell culture device that is easy to add liquid according to claim 1, characterized in that: Two fixing blocks (13) are fixedly connected to the cover plate (2), and a limiting rod (14) is slidably connected to the fixing block (13).

4. A cell culture device that facilitates liquid addition according to claim 3, characterized in that: A tension spring (15) is sleeved on the limiting rod (14), and a round cap (5) is fixedly connected to one end of the limiting rod (14).

5. The cell culture device for easy liquid addition according to claim 1, characterized in that: The culture dish (1) is provided with two positioning holes (11), and the culture dish (1) is provided with two limiting holes (12).

6. A cell culture device that facilitates liquid addition according to claim 5, characterized in that: Two positioning rods (16) are fixedly connected inside the cover plate (2), and the positioning rods (16) correspond to the positioning holes (11).

Images