A stem cell high-efficiency expansion culture device

Abstract

The utility model relates to petri dish technical field, concretely is a kind of stem cell efficient amplification culture device, including box, the inside fixed of box has inner enclosure, the top of inner enclosure is sleeved and is connected with cover body, the bottom of cover body is inserted between box and inner enclosure, the surface of box is equipped with through hole two, the surface of inner enclosure is equipped with through hole one;Beneficial effect is: the double-layer enclosure structure formed by the box and inner enclosure is used.Cover body is screwed on the top of inner enclosure, the stem cell cultivated in the inside of box can be effectively protected.Even if the box is damaged, since the existence of inner enclosure, still can provide a relatively independent, safe culture space for stem cell, avoid external pollutants to enter, thereby guarantee the activity and quality of stem cell, improve culture success rate.

Description

Technical Field

[0001] This utility model relates to the field of culture dish technology, specifically to a high-efficiency stem cell expansion and culture device. Background Technology

[0002] In the field of stem cell research and application, efficient expansion and culture of stem cells is a crucial step. Stem cells possess the potential for self-renewal and multi-lineage differentiation, showing great promise for applications in tissue engineering, regenerative medicine, and disease treatment. However, the stem cell culture process has extremely demanding environmental requirements, necessitating strict control of factors such as temperature, humidity, and gas composition to ensure the normal growth and proliferation of stem cells.

[0003] Currently, various stem cell culture devices exist on the market, but these devices generally suffer from several problems. Firstly, many devices lack adequate protection; during transportation, storage, or use, they are easily damaged by external impacts or accidental collisions, leading to contamination of the culture environment. This, in turn, affects the activity and quality of stem cells, and may even cause culture failure. Secondly, existing culture devices are not convenient enough for adjusting the gas composition in the culture environment. For example, switching from an aerobic to an anaerobic culture environment often requires additional equipment or complex operations, increasing the difficulty and cost of the culture process. Furthermore, when multiple culture dishes need to be cultured simultaneously, the stacking stability between the dishes is poor, easily leading to tilting or tipping, which not only affects the culture results but may also damage the culture dishes and waste stem cells. Utility Model Content

[0004] The purpose of this invention is to provide a high-efficiency stem cell expansion and culture device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a high-efficiency stem cell expansion and culture device, comprising a box body, an inner enclosure fixed inside the box body, a cover body screwed onto the top of the inner enclosure, the bottom of the cover body being inserted between the box body and the inner enclosure, a second through hole being formed on the surface of the box body, and a first through hole being formed on the surface of the inner enclosure.

[0006] Preferably, the height of the inner enclosure is equal to the height of the groove of the box body, and the box body and the inner enclosure form an annular groove. The inner ring surface of the cover body is provided with an internal thread, and the outer ring surface of the inner enclosure body is provided with an external thread. The external thread and the internal thread are connected in a mating manner, and the top surface of the cover body is provided with multiple finger grooves.

[0007] Preferably, both through holes one and through holes two are provided in multiples. Through holes one are arranged at equal intervals and of equal size along the outer ring surface of the inner enclosure, and through holes two are arranged at equal intervals and of equal size along the inner ring surface of the box.

[0008] Preferably, the outer circumferential surface of the cover has a marking groove at the top, and the marking groove is an annular groove.

[0009] Preferably, both the outer and inner ring surfaces of the cover are fixed with rubber rings at their bottom ends. One rubber ring is clamped between the cover and the box, and the other rubber ring is clamped between the cover and the inner enclosure.

[0010] Preferably, a rubber ring is fixed to the bottom surface of the top plate of the cover. The rubber ring has a U-shaped cross-section and is clamped between the top plate of the cover and the top surface of the inner enclosure.

[0011] Preferably, the bottom surface of the box body is provided with a stacking groove, the diameter of which is equal to the diameter of the top surface of the cover body.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] The stem cell high-efficiency expansion and culture device proposed in this invention adopts a double-layer protective structure consisting of a box and an inner enclosure. After the cover is screwed onto the top of the inner enclosure, it can effectively protect the stem cells cultured inside the box. Even if the box is damaged, the presence of the inner enclosure can still provide a relatively independent and safe culture space for the stem cells, preventing external contaminants from entering, thereby ensuring the activity and quality of the stem cells and improving the culture success rate.

[0014] This system enables convenient adjustment of the gas composition in the culture environment. A second through-hole is made on the surface of the box, and a first through-hole is made on the surface of the inner enclosure; the positions of both through-holes are carefully designed. After the cover is screwed onto the top of the inner enclosure, the positional relationship between the bottom surface of the marking groove and the top surface of the box allows determination of whether through-holes one and two form a pathway. When both are coplanar, through-holes one and two are connected, facilitating the supply of air to the box and meeting the aerobic culture requirements of stem cells. When anaerobic culture is required, simply continue screwing the cover downwards to seal through-holes one and two at the bottom. Simultaneously, a rubber ring is clamped between the top plate of the cover and the top surface of the inner enclosure, achieving a good seal and preventing outside air from entering, thus providing an anaerobic culture environment for stem cells. This adjustment method is simple and quick to operate, requiring no additional equipment, reducing culture costs and operational difficulty.

[0015] The bottom of the box has a stacking groove, the diameter of which is equal to the diameter of the top surface of the lid. When multiple sets of culture dishes need to be stacked, the stacking groove at the bottom of one set of culture dishes is placed over the lid at the top of another set, ensuring that the two culture dishes are not tilted and maintain a stable stacking state. This design not only improves the stacking efficiency of culture dishes but also avoids the problems of damage to culture dishes and waste of stem cells caused by unstable stacking, which is beneficial for large-scale stem cell culture experiments. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a top view of the structure of this utility model;

[0018] Figure 3 for Figure 2 Sectional view of the structure at point AA;

[0019] Figure 4 for Figure 3 Enlarged schematic diagram of the structure at point A in the middle;

[0020] Figure 5 This is a schematic diagram of the connection structure between the box body and the inner enclosure of this utility model.

[0021] In the diagram: 1. Box body; 2. Inner enclosure; 3. Cover; 4. Through hole one; 5. Through hole two; 6. Marking groove; 7. Rubber ring one; 8. Finger groove; 9. Stacking groove; 10. Rubber ring two; 11. Limiting ring. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this utility model clear and complete, the embodiments of this utility model will be further described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are only some, not all, embodiments of this utility model, and are merely used to explain the embodiments of this utility model. They are not intended to limit the embodiments of this utility model. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0023] Please see Figures 1 to 5 This utility model provides a technical solution: a high-efficiency stem cell expansion and culture device, including a box body 1. An inner enclosure 2 is fixed inside the box body 1. A cover 3 is screwed onto the top of the inner enclosure 2. The bottom of the cover 3 is inserted between the box body 1 and the inner enclosure 2. The height of the inner enclosure 2 is equal to the height of the groove in the box body 1. An annular groove is formed between the box body 1 and the inner enclosure 2. The inner annular surface of the cover 3 has an internal thread, and the outer annular surface of the inner enclosure 2 has an external thread. The external and internal threads are connected. Multiple finger grooves 8 are provided on the top surface of the cover 3. The box body 1 and the inner enclosure 2 form a double-layer protective structure. After the cover 3 is screwed onto the top of the inner enclosure 2, the stem cells cultured inside the box body 1 are protected. Even if the box body 1 is damaged, the inner enclosure 2 can still effectively protect the stem cells. The finger grooves 8 are provided to facilitate the insertion of fingers into the finger grooves 8 to manipulate the cover 3. A limit ring 11 is fixed to the inner wall of the inner enclosure 2.

[0024] The surface of the box body 1 has a second through hole 5, and the surface of the inner enclosure 2 has a first through hole 4. The first through hole 4 is located above the limiting ring 11. There are multiple first through holes 4 and second through holes 5. The first through holes 4 are arranged at equal intervals and of equal size along the outer ring surface of the inner enclosure 2, and the second through holes 5 are arranged at equal intervals and of equal size along the inner ring surface of the box body 1. The outer ring surface of the cover body 3 has a marking groove 6 at the top. The marking groove 6 is an annular groove. Both the outer ring surface and the inner ring surface of the cover body 3 have rubber rings 2 10 fixed at the bottom. One rubber ring 2 10 is clamped between the cover body 3 and the box body 1, and the other rubber ring 2 10 is clamped between the cover body 3 and the inner enclosure 2. The bottom surface of the top plate of the cover body 3 has a rubber ring 1 7. The rubber ring 1 7 is a circular ring structure with a "U" shaped cross section. The rubber ring 1 7 is clamped between the top plate of the cover body 3 and the top surface of the inner enclosure 2. When the cover 3 is screwed onto the top of the inner enclosure 2, and the bottom surface of the marking groove 6 is coplanar with the top surface of the box 1, the through hole 4 and the through hole 5 form a passage, which facilitates the delivery of air to the stem cells cultured inside the box 1. When it is necessary to perform anaerobic culture on the contents inside the box 1, continue to screw the cover 3 downwards until the bottom of the cover 3 seals the through hole 4 and the through hole 5. At this time, the rubber ring 7 is clamped between the top plate of the cover 3 and the top surface of the inner enclosure 2 to achieve a seal.

[0025] The bottom surface of the box 1 is provided with a stacking groove 9, and the diameter of the stacking groove 9 is equal to the diameter of the top surface of the cover 3. When multiple sets of culture dishes need to be stacked, the stacking groove 9 at the bottom of one set of culture dishes is placed on the cover 3 at the top of another set of culture dishes, so as to ensure that the two culture dishes will not be tilted when stacked.

[0026] Instructions for using this high-efficiency stem cell expansion and culture device:

[0027] Align the internal thread of the cover 3 with the external thread of the inner enclosure 2, and slowly rotate the cover 3 until it is screwed onto the top of the inner enclosure 2. During rotation, ensure that the bottom of the cover 3 is smoothly inserted between the box 1 and the inner enclosure 2. After the cover 3 is screwed onto the top of the inner enclosure 2, observe whether the bottom surface of the marking groove 6 is coplanar with the top surface of the box 1. If they are coplanar, it means that through hole 4 and through hole 5 form a passage, and subsequent culture operations can be performed. If anaerobic culture is required, continue to screw the cover 3 downwards until the bottom of the cover 3 completely seals through hole 4 and through hole 5. At this time, rubber ring 7 is clamped between the top plate of the cover 3 and the top surface of the inner enclosure 2 to achieve a seal and prevent outside air from entering. Place the stem cells to be expanded and cultured within the inner enclosure 2 inside the box 1, taking care to avoid contaminating the stem cells during the operation. When the bottom surface of the marking groove 6 is coplanar with the top surface of the box 1, through-hole 4 and through-hole 5 form a passage, allowing outside air to enter the interior of the box 1 and providing an aerobic culture environment for stem cells. At this point, conventional aerobic stem cell culture methods can be followed, such as regularly observing cell growth and replenishing culture medium. After further unscrewing the cover 3 to seal through-hole 4 and through-hole 5, the gas inside the box 1 is replaced with oxygen-free gas using appropriate gas replacement equipment, creating an anaerobic culture environment. Then, culture is carried out according to anaerobic culture operating procedures, such as strictly controlling temperature and humidity. When multiple sets of culture dishes need to be stacked, first ensure that each culture dish has completed the above-mentioned installation, sealing, and stem cell placement operations. Align the stacking groove 9 at the bottom of one set of culture dishes with the cover 3 at the top of another set of culture dishes, and slowly lower it so that the stacking groove 9 is completely secured to the cover 3. This stacking method ensures that the two culture dishes will not tilt and remain stable. When the stem cell expansion culture reaches the expected goal, the culture is terminated according to the corresponding operating procedures. First, rotate the cover 3 upwards to open the culture device and remove the cultured stem cells. Then, clean and disinfect the culture device for future use.

[0028] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A stem cell high-efficiency expansion culture device, comprising a box body (1), characterized in that: The box (1) has an inner enclosure (2) fixed inside. The top of the inner enclosure (2) is fitted with a cover (3) screwed on. The bottom of the cover (3) is inserted between the box (1) and the inner enclosure (2). The surface of the box (1) has a second through hole (5), and the surface of the inner enclosure (2) has a first through hole (4).

2. The stem cell high-efficiency expansion culture device according to claim 1, characterized in that: The height of the inner enclosure (2) is equal to the height of the groove of the box body (1). The box body (1) and the inner enclosure (2) form an annular groove. The inner ring surface of the cover (3) is provided with an internal thread, and the outer ring surface of the inner enclosure (2) is provided with an external thread. The external thread and the internal thread are connected in a mating manner. The top surface of the cover (3) is provided with multiple finger grooves (8).

3. The high-efficiency stem cell expansion and culture device according to claim 1, characterized in that: Both the first through hole (4) and the second through hole (5) are provided in multiples. The first through hole (4) is arranged at equal distances and of equal size along the outer ring surface of the inner enclosure (2), and the second through hole (5) is arranged at equal distances and of equal size along the inner ring surface of the box (1).

4. The high-efficiency stem cell expansion and culture device according to claim 1, characterized in that: The outer ring surface of the cover (3) has a marking groove (6) at the top, and the marking groove (6) is an annular groove.

5. The high-efficiency stem cell expansion and culture device according to claim 1, characterized in that: Both the outer and inner ring surfaces of the cover (3) are fixed with rubber rings (10) at the bottom. One rubber ring (10) is held between the cover (3) and the box (1), and the other rubber ring (10) is held between the cover (3) and the inner enclosure (2).

6. The high-efficiency stem cell expansion and culture device according to claim 1, characterized in that: A rubber ring (7) is fixed to the bottom surface of the top plate of the cover (3). The rubber ring (7) has a U-shaped cross-section and is sandwiched between the top plate of the cover (3) and the top surface of the inner enclosure (2).

7. The high-efficiency stem cell expansion and culture device according to claim 1, characterized in that: The bottom surface of the box (1) is provided with a stacking groove (9), and the diameter of the stacking groove (9) is equal to the diameter of the top surface of the cover (3).

Images