The invention discloses a preparation method of mulberry leaf tea, and the method comprises the following steps of: picking mulberry leaf, drying in a spreading way, roasting, cooling, twisting, extracting fragrance and packing, wherein the working procedure repeatedly comprises at least three times of steps of roasting, cooling and twisting, namely firstly roasting, cooling and twisting, secondly roasting, cooling and twisting, and thirdly roasting, cooling and twisting; and the temperature of a roasting boiler is 130-140DEG C, and the stir-frying time of the first roasting is 3-4 minutes. Compared with the prior art, the preparation method can be effectively removing the grass fishy smell, guaranteeing the nutrition, fresh and tender raw materials and even heating of the mulberry leaf when being roasted and optimizing the mouthfeel of the mulberry leaf tea, and is convenient to operate, low in cost, and good in economic benefit, the prepared mulberry leaf tea is fresh in mouthfeel, free from peculiar smell and fresh and green in color and luster, and the blood pressure and the blood fat can be effectively reduced after the mulberry leaf tea is drunk for a long term.