Sterile cultivation method for enteromorpha

Abstract

The invention relates to a sterile cultivation method for algae, in particular to a sterile cultivation method for enteromorpha. Firstly, enteromorpha seedlings are washed 2-3 times, and the surfaces of the enteromorpha seedlings are absorbed dry; secondly, three antibiotics including the penicillin G, the neomycin and the polymyxin B are added into a VSE nutrient solution to be prepared into an antibiotic nutrition solution; thirdly, the enteromorpha seedlings are placed in the antibiotic nutrition solution, the cultivation temperature ranges from 18 DEG C to 25 DEG C, the illumination intensity ranges from 130mumol / m<2>*s, the photoperiod is 12L:12D, the enteromorpha seedlings are cultivated in an inflation mode, and the antibiotic nutrition solution is replaced every 3-6 days until the enteromorpha seedlings grow into mature algae. The sterile cultivation is carried out on the enteromorpha collected in the field in a laboratory, the enteromorpha is tested and has no infectious microbe growth, and accordingly the accuracy of the later experiment results of the enteromorpha is greatly improved. The sterile cultivation method is easy to operate, the enteromorpha cultivation system does not need to be changed, raw materials are easy to obtain, price is low, and cost is low.

Description

technical field [0001] The invention relates to an aseptic culture method of algae, in particular to an aseptic culture method of enteromorpha. Background technique [0002] Since 2008, green tide has become one of the most harmful ecological phenomena in my country's coastal waters, causing serious damage to coastal economic development and ecological environment. The occurrence of green tide is caused by the explosive growth of green tide algae, the most important species of green tide algae is Enteromorpha. In plant taxonomy, Enteromorpha belongs to green algae. Although its plant body is very slender and looks like green filaments to the naked eye, it is large enough to be called "macroalgae". In recent years, the research on Enteromorpha has become an important direction in the field of green tide research. Enteromorpha often grows on rocks or bogs in the intertidal zone, or on gravels on muddy beaches, and sometimes grows on the algal bodies of large seaweeds. Unde...

AI Technical Summary

Problems solved by technology

[0003] At present, the aseptic culture of Enteromorpha mainly adopts disinfection and physical methods. However, both disinfection and physical methods require multiple changes to the existing Enteromorpha culture system, and there is a certain degree of difficulty in operation. achieve sterility

[0004] The antibiotic method is generally recognized for its rapid effectiveness and thoroughness of sterilization. The method of microalgae sterilization usually adopts the antibiotic method, but there is no relevant literature on the use of the antibiotic method in the aseptic culture of Enteromorpha. Pass