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A culturing method and technology for prolifera, which are applied in the field of aseptic culture of prolifera, can solve problems such as inability to achieve sterilization, and achieve the effects of good growth state, bright green color and improved accuracy.
Inactive Publication Date: 2014-03-05
SHANGHAI OCEAN UNIV
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Problems solved by technology
[0003] At present, the aseptic culture of Enteromorpha mainly adopts disinfection and physical methods. However, both disinfection and physical methods require multiple changes to the existing Enteromorpha culture system, and there is a certain degree of difficulty in operation. achieve sterility
[0004] The antibiotic method is generally recognized for its rapid effectiveness and thoroughness of sterilization. The method of microalgae sterilization usually adopts the antibiotic method, but there is no relevant literature on the use of the antibiotic method in the aseptic culture of Enteromorpha. Pass
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Embodiment 1
[0031] (1) Pretreatment of Enteromorpha seedlings
[0032] The surface of the Enteromorpha seedlings cultivated in the early stage was washed with sterile seawater for 3 times, and then the excess water on the surface of the algae body was blotted with sterile filter paper.
[0033] (2) Aseptic culture process
[0034] After the three antibiotics of penicillin G, neomycin and polymyxin B were dissolved, they were sterilized by microporous membrane filter sterilization method respectively, and the pore size of the microporous membrane was 0.22 μm.
[0035] Add 25mg of penicillin G, 25mg of neomycin and 12.5mg of polymyxin B into 250mL of VSE culture solution to prepare antibiotic culture solution.
[0036] Put the pretreated Enteromorpha seedlings in the antibiotic culture solution, and place them according to the ratio of adding 50mL antibiotic culture solution to each Enteromorpha seedling; then place them at a temperature of 20°C and a light intensity of 140μmol / m 2 s. Und...
Embodiment 2
[0040] (1) Pretreatment of Enteromorpha seedlings
[0041] The surface of the Enteromorpha seedlings cultivated in the early stage was washed with sterile seawater for 3 times, and then the excess water on the surface of the algae body was blotted with sterile filter paper.
[0042] (2) Aseptic culture process
[0043] After the three antibiotics of penicillin G, neomycin and polymyxin B were dissolved, they were sterilized by microporous membrane filter sterilization method respectively, and the pore size of the microporous membrane was 0.22 μm.
[0044] Add 25mg of penicillin G, 25mg of neomycin and 25mg of polymyxin B into 250mL of VSE culture medium to make antibiotic culture medium. Put the pretreated Enteromorpha seedlings in the antibiotic culture solution, and place them according to the ratio of adding 45mL antibiotic culture solution to each Enteromorpha seedling; 2 s. The light cycle is 12L:12D under the condition of aerated culture, and the antibiotic culture sol...
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Abstract
The invention relates to a sterile cultivation method for algae, in particular to a sterile cultivation method for enteromorpha. Firstly, enteromorpha seedlings are washed 2-3 times, and the surfaces of the enteromorpha seedlings are absorbed dry; secondly, three antibiotics including the penicillin G, the neomycin and the polymyxin B are added into a VSE nutrient solution to be prepared into an antibiotic nutrition solution; thirdly, the enteromorpha seedlings are placed in the antibiotic nutrition solution, the cultivation temperature ranges from 18 DEG C to 25 DEG C, the illumination intensity ranges from 130mumol/m<2>*s, the photoperiod is 12L:12D, the enteromorpha seedlings are cultivated in an inflation mode, and the antibiotic nutrition solution is replaced every 3-6 days until the enteromorpha seedlings grow into mature algae. The sterile cultivation is carried out on the enteromorpha collected in the field in a laboratory, the enteromorpha is tested and has no infectious microbe growth, and accordingly the accuracy of the later experiment results of the enteromorpha is greatly improved. The sterile cultivation method is easy to operate, the enteromorpha cultivation system does not need to be changed, raw materials are easy to obtain, price is low, and cost is low.
Description
technical field [0001] The invention relates to an aseptic culture method of algae, in particular to an aseptic culture method of enteromorpha. Background technique [0002] Since 2008, green tide has become one of the most harmful ecological phenomena in my country's coastal waters, causing serious damage to coastal economic development and ecological environment. The occurrence of green tide is caused by the explosive growth of green tide algae, the most important species of green tide algae is Enteromorpha. In plant taxonomy, Enteromorpha belongs to green algae. Although its plant body is very slender and looks like green filaments to the naked eye, it is large enough to be called "macroalgae". In recent years, the research on Enteromorpha has become an important direction in the field of green tide research. Enteromorpha often grows on rocks or bogs in the intertidal zone, or on gravels on muddy beaches, and sometimes grows on the algal bodies of large seaweeds. Unde...
Claims
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