56results about How to "Rich biodiversity" patented technology

The invention discloses a method for cultivating tea comprising the steps of, tea plantation sun screening, planting deciduous arbor around the tea plantation and between the tea trees, covering the tea plantation through rice straw and planting bean green manure, applying special-purpose fertilizer for organic tea, inoculating beneficial soil organisms and soil creatures including right amount of earthworm, finally carrying out irrigation through mini- sprinkling irrigation system in high temperature dry seasons.

The invention belongs to the technical field of livestock breeding and specifically relates to an ecological method of breeding chicken by planting grass under trees. The method includes that a fine forage grass breed which is shade-tolerant, trampling-resistant, strong in stress resistance and applicable to being planted in the forest is selected, and a forest land for grazing the chicken is modified to form a grassland under the trees for grazing the chicken; a contour grass and shrub zone in a stocking land is used for planting the grass, a chicken house is built in the middle of the grassland under the trees for grazing the chicken, zone division is performed for rotation grazing, and zones are separated by biological fences; and Chinese herbal plants are sowed in rotation grazing zones so that the grazed chicken can freely ingest the plants. A scientific evidence is provided for obvious economic benefits and ecological benefits which are achieved by means of rapid development of domestic fowls and effective ecological rehabilitation, wherein the domestic fowls of Hetian chicken and the like are applicable to stocking in forestland. By means of the model for breeding the chicken, high economic benefits are created, parts of the economic benefits are used for investing development of ecological chicken breeding model, and the created economic benefits and the development of ecological chicken breeding model are combined for creating better benefits, and accordingly, a virtuous cycle is achieved, ecological balance is kept, and the sustainable development is effectively achieved.

The invention belongs to the field of agricultural environmental protection, and particularly relates to a method for establishing nature-simulated ecological farmland. According to the method, the farmland, trenches, a tractor-ploughing road, farmland buffer zones and a buffer purification pool are established; on the basis of a farmland production function, a farmland ecological function is further exerted, the farmland is utilized for consumption and sinks of environmental nitrogen and phosphorus; through soil carbon sinks on the farmland and biodiversity, non-point source pollution to the farmland is controlled. Through adoption of the method, the functions of the farmland, farmland ridges, the trenches, the tractor-ploughing road and the buffer purification pool in a whole compound system are optimized; the farmland biodiversity is improved, and a farmland planting and breeding compound function and a pollution source control function are extended; farmland ridge buffering, beautifying and economic functions are added, and the non-point source pollution self-purification capability of the farmland is enhanced; the trenches are hardened through ecologicalization, and nitrogen and phosphorus nutrients can be recycled; interception and purification of the non-point source pollution to the farmland are regulated and controlled; an ecological corridor is established, farmland landscapes are beautified, and the ecological value of the farmland is improved.

The invention provides an ecological ditch with a nitrogen and phosphorus removal function. The ecological ditch with the nitrogen and phosphorus removal function comprises a quasi-three-smooth-side ditch body, a plurality of solid slow release carbon source packing pools and a lifting dam, wherein the solid slow release carbon source packing pools are located below the bottom of the quasi-three-smooth-side ditch body, and the lifting dam is located at the tail end of the ecological ditch in the water flow direction. On the whole, a construction method of the ecological ditch with the nitrogen and phosphorus removal function is developed by taking the problems of a traditional soil ditch and a traditional three-smooth-side cement hardened ditch into full consideration. On the basis that the normal drainage function of a farmland ditch is guaranteed, the functions of a soil adsorption part, a packing adsorption part, a plant adsorption part, a biodegradation part and other parts of the ditch are enhanced, the biological diversity of the farmland ditch is enriched, effective removal of nitrogen and phosphorus pollutants in farmland tail water is achieved, and the ditch is made to serve as a nitrogen and phosphorus pollutant treatment unit between the farmland tail water and a receiving water body.

The invention discloses a construction and building method of an anti-pollution mangrove forest protection forest. The construction and building method comprises the following steps that 1-2-year seedling mangrove plant species with the standard municipal sewage rate being more than 50% are selected according to municipal sewage standards; annular pagatpat annual or laguncularia racemosa biennial seedling forest zone with the width being 15m-20m is planted and constructed on mudflats which have more serious offshore pollution and great storms and are unsuitable for afforestation; after a pagatpat or laguncularia racemosa forest is planted for 2-3 years, the mangrove plant species are planted in a space in an offshore annular zone with the width being 15m-20m; the plant spacing and the row spacing for planting pagatpat or laguncularia racemosa are 1m-2m and 1m-2m respectively; the plant spacing of planting a local mangrove forest is 0.5m-1m and the row spacing is 0.5m-1m. According to the construction and building method, the mangrove forest is built on the mudflats which have more serious offshore pollution and great storms and are unsuitable for afforestation, so that the local mangrove forest can be built while exotic species are planted; the landscape ecology is coordinated and the species are diversified and abundant; the mangrove forest is high in survival rate and has good anti-pollution and anti-storm performances.

The invention discloses a construction and creation method of an indigenous mangrove protection forest on a foreshore unsuitable for afforestation. The method includes the steps: a, planting an annual seedling forest of sonneratia apetala on the heavy-wind-wave foreshore, unsuitable for afforestation, 15-20 meters from a coast to construct a mangrove protection wall resisting wind waves, wherein planting distance of the sonneratia apetala is 1-2m, and line spacing is 1-2m; b, after the sonneratia apetala forest is planted for 2-3 years, planting biennial indigenous mangrove species on a vacant lot of a sonneratia apetala planting field, wherein planting distance of the indigenous mangrove species is 0.5-1m, and the line spacing is 0.5-1m. Exotic species and the indigenous species of mangroves are created on the heavy-wind-wave foreshore unsuitable for afforestation, restored mangrove wetland has rich biologic diversity and a good ecological function, coastal landscape is improved significantly as compared with that of a traditional forestation method, respective benign growing environment of the exotic species and the indigenous species is maintained, and normal growth of the indigenous species is unaffected; meanwhile, the indigenous mangrove protection forest has good wind wave resistance and functions of dam protection, siltation promotion and the like.

The invention discloses a method for constructing and building arbor wave-eliminating forest belt on non-forestry river mouth sludge intertidal zone beach, wherein the method belongs to the technical field of agricultural cultivation. The method comprises the following steps of A, manual ditching and fetching soil for manufacturing piers and lifting ground; B, planting arbor container seedlings; C, performing mechanical soil working and planting a mangrove forest; D, planting intersected arbors in a mangrove planting area; and E, performing ditch cleaning and supporting, thereby improving tree growth and beach siltation. The method of the invention has functions of realizing normal growth of arbor trees such as Casuarina equisetifolia on the wetland of the non-forestry river mouth sludge intertidal zone beach, improving survival rate of the protection forest, successively applying creation of wave-eliminating forest belt on the wetland of the non-forestry beach, facilitating restoring and reconstruction of the biological system on the wetland of the non-forestry beach, facilitating resistance for great natural disasters such as typhoon and flooding tide at coastal regions, and performing a positive function on economic development of Chinese sea.

The invention provides a riverway microhabitat reforming and water quality purifying device. The riverway microhabitat reforming and water quality purifying device comprises a plurality of wooden piles arranged on the river bank. Block stone is inlaid between the wooden piles. The wooded piles and the block stone define a periphery structure. The wooden piles are gradually shorter in the water flow direction of the riverway. The block stone, gravel and silt are sequentially laid inside the wooden piles and form an aquatic plant plantation base material. In the water flow direction of the riverway, emergent aquatic plants and submerged plants are sequentially planted on the silt. The riverway microhabitat reforming and water quality purifying device can purify water quality, provide inhabitant and propagation space and bait sources for aquatic organisms, enrich the biological diversity of the riverway and form a submerged forest landscape effect, has high flood fighting capacity, can manufacture complicated flow state and has high discharge capacity.

The invention discloses a method for improving the capability of a hardened ditch in removing nitrogen-phosphorus contaminations in tail water of farmland. The method comprises the following concrete steps: constructing a plurality of small filler-plant treating units in the ditch along the two sidewalls of the ditch and arranging an elevating dam at the tail end of the trench, wherein each of the small filler-plant treating units successively comprises an adsorption filler water-permeable dam and a pine pile vegetation zone along a water flow direction, and the adsorption filler water-permeable dam and the pine pile vegetation zone are closely bonded. According to the invention, the tail water of the farmland is allowed to fully contact with water plants and an adsorption filler, so plant absorption, microbial degradation and filler adsorption of contaminations in the ditch are reinforced; the height of the elevating dam can be adjusted according to changing between an irrigation period and a non-irrigation period, so the ditch is rapidly switchable between a flood draining function and a contamination treating function; thus, the hardened ditch is reconstructed into a ditch with good capability in removing nitrogen-phosphorus contaminations and realizes removal of non-point source agricultural pollution.

The invention discloses a paddy rice no-tillage direct-sowing method characterized in comprising the steps of (1) weeding, (2) seed treatment, (3) sowing, (4) secondary weeding, (5) fertilizing, and (6) secondary sowing. With the method, soil organism growth and development are promoted, soil biodiversity is enriched, chemical fertilizer use is reduced, farmland non-point source pollution is reduced, labor and effort are saved, agricultural planting efficiency is improved, and paddy rice quality is improved.

The invention provides application and a preparation method of a novel high-universality efficient composite carbon source. The novel high-universality efficient composite carbon source comprises a dominant carbon source for denitrifying bacterium growth, a growth promoting agent and anaerobic bacteria, and the growth and denitrification performance of denitrifying bacteria can be promoted. The carbon source accounts for 65%-75% of the total amount of the composite carbon source and comprises 45%-60% of small-molecular acid, 10%-20% of small-molecular alcohol, 5%-10% of saccharides, 5%-10% of sodium acetate and 0-30% of water, summing to 100%. The growth promoting agent accounts for 0.5%-5% of the total amount of the composite carbon source and comprises inorganic salt, compound enzyme, compound amino acid, compound vitamin and tap water, wherein the inorganic salt accounts for 0.1%-2%, the compound enzyme accounts for 0.5%-5%, the compound amino acid accounts for 0.5%-5%, the compound vitamin accounts for 0.5%-5%, and the tap water accounts for 83%-98.4%, totaling to 100%. The anaerobic bacteria account for 0.5%-5%. The COD of the composite carbon source provided by the invention is 500,000-600,000 mg / L. A high-universality efficient composite carbon source nutritional agent has the characteristics of high efficiency, environmental protection performance, stability and convenience in use, and can well adapt to and meet market requirements.

The invention discloses a method for planting calliopsis on a side slope. The method comprises the following steps: preparing slope surface; backfilling nutrient soil on the slope surface, and laying non-woven fabrics or coarse sand on the nutrient soil; pouring ecological concrete and covering a plastic thin film for maintenance, wherein the ecological concrete is prepared from gravels, cement, micro silicon dioxide powder or fly ash, a water reducing agent and water according to a weight proportion by uniform mixing under stirring; sowing calliopsis seeds and carrying out watering for maintenance, wherein the calliopsis seeds are pretreated through the following steps before sowing: adding starch and 6-benzyl aminopurine into an extract obtained by mixing rhubarb and traditional Chinese medicinal clove, then carrying out stirring so as to obtain an emulsion, and soaking the calliopsis seeds in the emulsion for use. The method provided by the invention enriches diversity of plants cultivated on the ecological concrete; meanwhile, the calliopsis seeds undergo soaking pretreatment in the emulsion of the extract containing rhubarb and traditional Chinese medicinal clove before sowing, so the disease-resisting ability and the survival rate of the calliopsis seeds are improved.

The invention discloses a method for producing green tea in autumn, which comprises the following steps: a special fertilizer for organic tea is applied; mechanical picking or labor picking with limitation is carried out and the picking strength is to cut 1 / 2 of new shoot growing in autumn; a trellis is put up in the tea garden and a shading net is covered to conduct shading; low temperature (24 DEG C to 26 DEG C) drying is carried out; green removing is carried out when the temperature of the inlet barrel of a fixation machine is 195 DEG C to 215 DEG C, the air temperature at the tea inlet is 100 DEG C to 110 DEG C and the temperature of the outlet barrel is 145 DEG C to 155 DEG C; and drying and shaping are conducted in a curing machine, the temperature is controlled to be the temperature of the air intake at the beginning, tea is thinly laid in a plate made of bamboo sheets to be cooled until the surface of the tea is colloidal and pekoe shows out. The method is simple, has low cost, increases the production of the green tea in hillside tea garden, obviously improves the quality of the tea in autumn and is conducive to conduct scale production of high quality green tea in autumn in the hillside area of Yangtze River.

The invention belongs to the field of riverway water body ecological remediation, and particularly relates to an ecological permeable spur dike system. The ecological permeable spur dike system is composed of a plurality of ecological permeable spur dikes, a gabion grid is placed on the inner side of a spur dike pile column, the inner part of the gabion grid is filled with absorption filling materials, and aquatic plants are planted on the upper layer. According the ecological permeable spur dike system, the function that river banks are not directly washed by incoming flows is achieved, the action that pollutants such as nitrogen and phosphorus in river water are held back and absorbed is achieved, riverway water quality can be improved, conditions are created for growth and inhabitationof aquatic organism, restoration of the riverway partial ecological environment is promoted, and biodiversities are increased.

Disclosed is a high-value permanent forest cultivation technology with functions of land utilization and land maintenance. According to the technology, the design and setting of 3 or more tree species of 6 types of various matching trees which contain soil fertility, can naturally improve fertility and are suitable for sites are adopted, and advanced breeding achievements and forest culture and management technologies are utilized for new technology survey design of land utilization and land maintenance, diameter-containing groups and other new indexes; existing forests are subjected to selective cutting; the tree species of 6 types are classified, selected and set; soil preparation for afforestation or interplanting is conducted; tending management is conducted; target trees are classified; thinning is conducted; regeneration under a selection system is conducted, and cultivated forests have forest sustained-yield management natural capabilities like natural forest land fertility improvement, natural seeding self-renewal development, rich biodiversity, and resistance to diseases, insect pests and natural disasters. With every 10-20 years as one management period, permanent forests are formed, wherein the permanent forests continuously have large-diameter timber precious and high-value output, crown canopies of mixed, uneven-aged, multi-layer and multi-tree-species towering trees permanently cover forest land, and the permanent forests have high economic, ecological, socioculture and landscape value. The problems that large-area abies holophylla pure forests in the southern regions have declined soil fertility and lower productivity are solved.

The present invention discloses a remote authenticating system for a biologic sample comprising a data sampling subsystem, a data preparing subsystem and a data displaying subsystem, wherein the data sampling subsystem is connected to the data preparing subsystem through internet, and the data preparing subsystem is connected to the data displaying subsystem through internet. The data sampling subsystem converts the biologic sample's image, images and voices in the sampling spot of the biologic sample into electric signals which can be transmitted by the network, and sends these signals to the data preparing subsystem through internet; the data preparing subsystem transfers the images and voices sampled in the data sampling subsystem to the data displaying subsystem; and the data displaying subsystem decodes the received audio-video signals from the data preparing subsystem, converts them into images and voices and plays the images and voices. The inventive remote authenticating system for a biologic sample can realize quick and remote authentication fro the biologic sample.

The invention discloses a method for cultivating tea comprising the steps of, tea plantation sun screening, planting deciduous arbor around the tea plantation and between the tea trees, covering the tea plantation through rice straw and planting bean green manure, applying special-purpose fertilizer for organic tea, inoculating beneficial soil organisms and soil creatures including right amount of earthworm, finally carrying out irrigation through mini- sprinkling irrigation system in high temperature dry seasons.

The invention provides a riverway ecological restoration system and restoration method, and belongs to the technical field of riverway restoration. The riverway ecological restoration system comprisesa riverway, the riverway comprises shoals and deep pools, the shoals comprise slow flow shoals and rapid flow shoals, a spur dike (1) is arranged at each rapid flow shoal, a bottom protection fixinggroove (2) is arranged at each deep pool, and aquatic plants (3) are planted at the slow flow shoals. According to the riverway ecological restoration system, by changing the waterpower condition of the riverway, the topographic characteristic that deep pools and shoals are alternated is formed, animals can inhabit conveniently, a healthy food chain of the river can be constructed, a riverway foodnet is formed, and the biodiversity of the riverway and the surroundings is enriched. The restoration method of the riverway adopts an ecological means, so that the ecological system of the riverwayis kept in a balanced state, the biodiversity is maintained, and the method is suitable for large-scale popularization and application.

The invention discloses an artificial channel drainage sandbeach sedimentation reducing system, and belongs to the technical field of coastal wetland ecological restoration. The artificial channel drainage sandbeach sedimentation reducing system is arranged on the silt beach side of an open sea and comprises a water injection ditch, a drainage passageway, outer walls and a water injection system.The water injection ditch is arranged on the rear land of the silt beach, the water injection system and the water injection ditch cooperate with each other, water is injected into the water injectionditch all the time, the outer walls are arranged on the two sides of the water injection ditch, water in the water injection ditch is drained to sandbeaches on the two sides through the drainage passageway; fine-particle mud in the drainage passageway is started under the action of water flow and moves, the mud is output along with offshore flow in a tide falling period, and sandbeach argillization is avoided. According to the artificial channel drainage sandbeach sedimentation reducing system, the ditch is artificially excavated, and the function of waves and tide is effectively adjusted toreduce natural hazards. Pollution can be effectively degraded through coast wetland restoration, and the primary ecological system and natural balancing mechanism are restored; and by means of ecological restoration, the diversity of the species is improved. A good basis is provided for further construction of leisure tourism inhabiting facilities, and a basis is provided for tourism development.

The invention belongs to the technical field of environment molecular biology and relates to a method for efficiently extracting microorganism total RNA (Ribonucleic Acid) from anaerobic granular sludge. The method comprises the following steps: fetching activated sludge, centrifuging, discarding supernatant, then adding sterilized glass beads and a stock solution I into a centrifugal tube, putting the centrifugal tube in a vortex mixer for beating after a tube cover of the centrifugal tube is screwed tightly, centrifuging to discharge supernatant, and washing by a phosphate buffer solution; adding a stock solution II and incubating; adding a TRIzol, centrifuging to obtain supernatant after vortex oscillation, adding chloroform, carrying out vortex oscillation, putting in a room temperature, centrifuging to obtain supernatant, adding isopropanol, putting in a temperature of -20 DEG C, centrifuging to discharge supernatanth, adding 75% ethanol to wash precipitates, centrifuging to discharge supernatant, air-drying the precipitates, dissolving nucleic acid precipitates by a stock solution III, adding DNase I (RNase free) and RNase inhibitors and adopting an RNA purifying reagent kit to purify the RNA. The steps for extracting the microorganism total RNA are simplified, and the microorganism total RNA is quickly and efficiently extracted from the anaerobic granular sludge.

The invention relates to a camellia sinensis planting method. According to the method, arbores that grow slowly are planted at the edge of a camellia sinensis planting area, the arbores are 2 to 3 meters away from camellia sinensis at the outmost periphery, a one-row and one-plant planting mode or a two-row and two-plant planting mode is adopted, and organic fertilizers such as decomposed manure of poultries and livestock as well as retting straws are utilized. The beneficial effects are that a problem that biocenoses singly rely on camellia sinensis is solved, rich biological diversity is provided, and large-scale insect pests are less likely to break out; pesticides and fertilizers are prevented from being excessively used during plantation of camellia sinensis; and produced tea is rich in selenium, popular in markets, and high in output value.

The invention discloses a rapid construction method of a coastal wind prevention and sand fixation salt-tolerant landscape community, and belongs to the technical field of ecological restoration. The rapid construction method of the coastal wind prevention and sand fixation salt-tolerant landscape community comprises the following steps: site investigation; site division; soil improvement; screening of salt-tolerant plants; quickly constructing a salt-tolerant landscape community; and performing tending management. The screened salt-tolerant plants are different in shape, rich in flower leaves and high in ornamental value, the plants of different salt-tolerant grades are subjected to echelon configuration of forest belts with different functions and three-dimensional matching of trees, shrubs and grass, the purposes of wind prevention, sand fixation and salt resistance can be effectively achieved, and a good ecological landscape effect is rapidly formed; the construction method is simple, convenient and practical, tending management is simple, a coastal vegetation community stable in structure and rich in biodiversity can be formed after two years of maintenance, lasting restoration and soil improvement of the coastal saline-alkali soil are achieved, and the method is suitable for being popularized in the Pearl River Delta and even the South China coastal area.

The invention discloses a composite tag for high-throughput sequencing of biological diversity of brewing fungi and application of the composite tag. Sequences for sequencing are shown as in following NO. 1 to NO. 48. An application method of the tag comprises: extracting total DNA of each sample; for each sample, using any pair of sequences from 48 pairs of unselected sequences as primers to amplify the total DNA of the sample; electrophoretically detecting an amplification product of each sample, and purifying and recovering the amplification products; precisely quantifying purified and recovered products of all samples with an ultraviolet spectrophotometer; mixing equivalently all samples to establish a sequencing library; subjecting the established sequencing library to high-throughput sequencing. The sample throughput of high-throughput sequencing can be increased, high-throughput sequencing cost can be effectively reduced, fungal populations in the whole microbial population in the samples can be effectively enriched, and a high-throughput analytical way is provided for the study on fungal diversity of an environment.

The invention provides a no-tillage direct seeding and mulching cultivation method of rice in the technical field of agricultural planting. In the cultivation method, Chinese milk vetch serves as green manure in winter, and is mulched on the ground surface as organic green manure before the seeding of the rice; and all rice straws are returned to the field, and are mulched on the ground surface after the rice is harvested; therefore, the permanent no-tillage of a rice field and the direct seeding of the rice are put into practice, and thus the crop rotation of the rice and the green manure are put into practice. When the method is adopted, the growth and development of soil organisms are improved; the diversity of the soil organisms is enriched; the application of fertilizers is reduced; the widespread pollution of a farming land is reduced; labor and force are saved; the agricultural planting efficiency is improved; and the quality of the rice is improved.

The invention relates to an ecological landscape floating island for riverway water quality restoration. The ecological landscape floating island comprises a floating island body, a stirring mechanism, a water pumping mechanism and a through pipe, wherein a restoration water tank is arranged in the floating island body, the stirring mechanism is arranged in the restoration water tank, the water pumping mechanism is arranged on the floating island body, a water inlet of the water pumping mechanism extends into the restoration water tank, a water outlet of the water pumping mechanism extends outof the floating island body, one end of the through pipe is communicated with the exterior of the floating island body, and the other end of the through pipe is communicated with the interior of therestoration water tank. The beneficial effects of the ecological landscape floating island are that: reagents and river water can be fully mixed through the stirring mechanism, the phenomenon that thereagents precipitate when the river water is improved is avoided, then the mixture of water and the reagents is discharged into a river channel through the water pumping mechanism, and compared witha traditional mode that the reagents are directly poured into the river, the efficiency in the river channel water quality improving process is improved.

The invention discloses a method for cultivation of rice in a rich-zinc formula. A disease-resistant variety and four special rice varieties, including giant embryo rice, black rice, glutinous rice and red rice, similar in growth period and different in plant height are adopted as hybrid cultivation varieties. By a rich-zinc cultivation method in which controlled-release zinc fertilizers are placed at a water inlet, produced rice in the rich-zinc formula is high in zinc content, and functional rich-zinc formulated rice can be obtained without adoption of a mixing formula for rice harvested ina maturation stage and are rich in functional components including zinc, GABA, vitamins, minerals, anthocyanidin, carotene, cardiac glycoside and the like, low in amylose content, soft, glutinous anddelicious in taste and high in market price, so that peasants can obtain high planting economic benefits. Mixed planting of different varieties makes full use of height differences of different rice varieties, light, heat and fertilizer resources can be fully coordinated, biodiversity is realized, disease and pest resistance is improved, pesticide consumption is reduced, and rice and soil contamination is abated.

The invention reLates to the fieLd of tea tree pLanting, in particuLar to a tea tree pLanting method. The pLanting method comprises the foLLowing steps of 1, Land seLection and preparation; 2, fruit tree interpLanting; 3, tea tree pLanting. According to the interpLanting mode, on the one hand, tea tree nutrient substances are not robbed, so that tea tree growth is not affected; on the other hand,resources are fuLLy utiLized, the benefit of a tea garden is increased, the tea garden organism diversity is enriched, the ecoLogicaL baLance of the tea garden is improved, and then disease and pest damage in the tea garden is reLieved.

The invention discloses a copper-cadmium heavy metal polluted site energy plant ecological intercepting, purifying and repairing system which comprises an isolation wall body arranged on the periphery of a polluted site in a surrounding mode, a plurality of liquid passivator filling modules distributed in the polluted site and energy plants planted on the polluted site. The liquid passivator filling module is used for uniformly adding a liquid passivator into the soil of the polluted site, the energy plants are planted in the soil of the polluted site and are cultivated and managed, and after the energy plants grow to the mature period, the overground parts of the energy plants are harvested or the energy plants are integrally removed from the polluted soil. The invention further discloses a working method of the system. Normal ecological and economic functions of the polluted land are brought into full play, high-valued utilization of the heavy metal polluted land is achieved, energy plants are vigorously developed through the heavy metal polluted land, soil heavy metal pollution can be effectively remedied, the way that heavy metal enters a food chain can be cut off, and food safety is guaranteed.

The invention discloses a rice three-free cultivation method for the paddy field. The rice three-free cultivation method comprises the following steps of treating straws of previous harvested crop, performing ridging, transplanting rice, adding earthworms, performing field management, and after rice is harvested, performing three-free circular planting. Ridging is performed in the paddy field, water is stored in furrows, and the rice is planted on ridges, so that the roofs of the ridges are maintained to be moist and free from water; through shielding of the rice, before ridging, an organic fertilizer is basally applied, full dose of the straws are returned to the field, and the roofs of the ridges are covered with the straw, so that favorable places and rich baits are provided for growthand propagation of the earthworms, and self-reproduction of the earthworms in the paddy field is realized. The earthworms are bred in the paddy field, so that the content of nitrogen, phosphorus, potassium and the like in the soil are increased, and the problems of sealing of soil, secondary gleyization and the like can be solved; through the furrows, the water storing and draining capacity of thepaddy field can be improved, the utilization efficiency of natural rainfall can be improved, and three-free operations namely free from tillage, free from straw burning and free from irrigation of the field, in the production course of the rice, are realized, so that the energy consumption during operation is reduced, the irrigation water is reduced, and haze is reduced.

The invention discloses a composite tag for high-throughput sequencing of biological diversity of fungi in an environment and application of the composite tag. Sequences of the composite tag are shown as in NO. 1 to NO. 48. An application method of the tag comprises: extracting total DNA of each sample; for each sample, using any pair of sequences from 48 pairs of unselected sequences as primers to amplify the total DNA of the sample; electrophoretically detecting an amplification product of each sample, and purifying and recovering the amplification products; precisely quantifying purified and recovered products of all samples with an ultraviolet spectrophotometer; mixing equivalently all samples to establish a sequencing library; subjecting the established sequencing library to high-throughput sequencing. The sample throughput of high-throughput sequencing can be increased, high-throughput sequencing cost can be effectively reduced, fungal populations in the whole microbial population in the samples can be effectively enriched, and a high-throughput analytical way is provided for the study on fungal diversity of an environment.