35 results about "Casuarina" patented technology

The invention relates to microbial fertilizer specially used for casuarinas. The invention is characterized in that the invention comprises liquid bacterial fertilizer which is obtained by mixing the fermentation broth of a Bacillus megaterium de Bary (Bacillus megaterium de Bary), a fermentation broth of a Frankia.sp (Frankia.sp) and stable effect promoter in a volume ratio of 1 to 1.5: 1.5 to 2.0: 0.1-0.5. The components of the stable effect promoter are counted by a mass fraction: 0.01 to 0.05 percent of flavonoids, 0.001 to 0.01 percent of coumarin, 0.001 to 0.01 percent of sodium molybdate, 0.001 to 0.01 percent of cobalt sulfate; the rest is water. The stable effect promoter also comprises a zeolite powder and organic fertilizer. The fertilizer can promote the growth of the casuarinas and increase the survival ratio and preservation ratio of the casuarinas as well as increasing the quantity of the casuarinas seedling with a nodule. Therefore, the resisting performance and the drought-resistance ability of the casuarinas are promoted. The crown closure speed of the plantation is increased. The windbreak and sand fixation effect of the casuarinas in the coastal protection forest is strengthened. The strain CCTCC M 207197 of Frankia.sp fgc6 provided by the invention has a better effect.

The invention mainly relates to the technical field of planting, and discloses a novel watermelon seed dressing agent. The watermelon seed dressing agent is prepared from the raw materials: nandina domestica, cedar needles, cortex erythrinae, water Caltrap shells, Illicium simonsii leaves, casuarina, Chukrasia tabularis, bombax roots, Jasminum multiflorum, Jasminum officinale, lignum vitae, compound enzyme, mint extract, uric acid, matrine alkali powder of which the concentration is 1.1% and zeatin; the novel watermelon seed dressing agent is rich in raw materials, safe and non-toxic, faint in scent and free of peculiar smells, convenient to use and less in use amount, further added with a variety of Chinese herbal medicines, extracted from pure plants and rich in nutrition and has a role of spectrum sterilization, so that micromolecule nutrients required for germination of watermelon seeds are provided to promote germination, and the sterilization and insect resistant rates both reach 86.2%; a variety of nutritional extractants can keep the embryo vigor of the watermelon seeds and enhance the stress resistance of watermelon seedlings; the uric acid is taken as a harmful body metabolite and can promote the watermelon seeds to germinate, so that wastes are turned into useful resources and utilization ways of the uric acid are increased; by adopting the novel watermelon seed dressing agent, the germination rate of the watermelon seeds reaches 97%, the watermelon yield is increased by 11.4%, and the planting cost is saved by 9.6%.

The invention provides a calcium organic fertilizer specially for preventing and treating peanut blight and a preparation method of the calcium organic fertilizer. In the invention, an aerobic high-temperature composting mode is adopted, the raw material formula of the calcium organic fertilizer is as follows: the calcium organic fertilizer comprises the following components by weight percentage:35-45% of pig manure, 8-12% of casuarina, 8-12% of peanut shell, 10-14% of shell powder, 3-6% of calcium superphosphate, 20-30% of water and 0.2-0.5% of effective microorganism (EM) agent. The pig manure is processed by a dry-wet separation technique. By applying regulation and control technologies such as moisture, temperature, pH value, C / N (carbon / nitrogen), C / P (carbon / phosphorus) and the like, based on sprouting index and C / N as maturity evaluation standards, the produced calcium organic fertilizer can meet the national standard of commercial organic fertilizer. In a sand ground peanut blight area along a shore, a special calcium peanut organic fertilizer is used, thereby effectively reducing the peanut blight rate and improving the economic benefits of peanuts.

The invention provides a method for of cultivating biomass, comprising the steps of: (a) Selecting at least a plant variety from the plant genus Casuarina capable of growing at a density equivalent to at least 5,000 stems per hectare for a period of 2 to 5 years; (b) Cultivating the plant variety for two to five years under suitable conditions to maintain the plantings at a density equivalent to at least 5,000 stems per hectare; and (c) Harvesting the resultant biomass between 2 and 5 years from the date of initial plantings.

The invention discloses a greenhouse pollination hybridization control method for casuarina. The method comprises the steps as follows: the male parent and the female parent of casuarina are grafted to obtain dwarfing hybridization parent plants; a pollination control chamber is built; and after blooming, the method of factorial mating is adopted to ensure that dwarfing hybridization parent plants of different types are pollinated and hybridized freely inter- or intra-species and are moved out from the pollination control chamber to go on growing after female flowers fade completely, so that the hybridization filial generation can be obtained. According to the invention, through tree selection of the dwarfing casuarina parent plants and pollination control of the pollination control chamber, the problems of high cost and low security caused by pollen collection or frame building pollination of large trees in the field can be avoided, and operability of hybridization pollination can be improved; besides, huge material cost and labor capacity of pollen collection and stage, female flower bagging, artificial pollination and the like in the traditional cross breeding can be saved, the detect of very low fruit setting rate and fruiting rate caused by adopting the conventional bagging pollination hybridization method can be overcome, wherein the fruit setting can reach over 90% and the fruiting rate can reach over 50%, the yield of hybridization seeds of casuarina can be increased greatly, and the cross-breeding efficiency can be improved.

The invention belongs to the technical field of seedlings raising and discloses a method for promoting germination of lateral branches of casuarinas. The method comprises the following steps: utilizing large trees and old trees as material; promoting lateral branches of old trees or big trees of casuarinas to germinate new and small branches. The method comprises the methods of pulling small branches, pressing branches, chopping branches by half and inverting branches. The method is capable of germinating new branches in batch for lateral branches of casuarinas. The number of newly-germinated branches is almost equal to that of small branches originally growing on lateral branches before technical processing. The method for promoting germination of lateral branches of casuarinas has the following beneficial effects: roots easily grow out of new and small branches growing out of lateral branches of casuarinas than original and old branches; rooting rate can be up to more than 80%; and the rooting rate of old and small branches of casuarinas is almost zero.

The invention relates to an aspergillus strain capable of promoting root growth of Casuarina. The strain is Casuarina rhizosphere fungi 63 of Aspergillus sp., and is registered and preserved in China General Microbiological Culture Collection Center on June 28, 2012, with an accession No. being CGMCC No. 6311. The Casuarina rhizosphere fungi 63 is inoculated on hydroponic seedlings of the Casuarina, and a comprehensively evaluation is carried out according to various indicators of root growth, and promotion effects of the fungi on root growth is further confirmed, thereby finding a functional endophytic fungi which is benefit to promote root growth and can be applied in production.

The invention discloses a method for carrying out spilling separation on ralstonia solanacearum of casuarina. The method comprises the following steps of digging a casuarina disease root which is 8 to 15 cm in length, washing cleanly, and cutting two ends of the casuarina disease root into flat new sections; then soaking one part, which is 3 to 5 cm in length, of one end into sterile water for 4 to 12 hours, and enabling milky bacterial ooze to flow out from the section on the other end of the casuarina disease root; picking the bacterial ooze by using an inoculating needle, carrying out streak cultivation on a bacteria culture medium, and carrying out amplification culture by selecting a separated typical single colony. The method for carrying out spilling separation on the ralstonia solanacearum of the casuarina, disclosed by the invention, has the advantages of easiness in operation, simpleness, convenience and quickness, high reliability and low content of infectious microbes.

The invention relates to a forestry tree seedling technology, and especially relates to a method for growing seedlings of casuarina branchlets into multiple segments. The method comprises: cutting casuarina branchlets whose length is larger than 10 cm into a plurality of segments of branchlets whose length is 5-6 cm; using a rooting liquor to soak the bases of the branchlets; and after root culture of the branchlets, planting seedlings. Beneficial effects are that a casuarina branchlet whose length is larger than 10 cm is cut into branchlets in length of 5-6 cm, the branchlet including a tip branchlet, a middle segment branchlet, and a base segment branchlet. The middle segment branchlet and the base segment branchlet, as well as the tip branchlet which has top end advantages, can be cultivated to normal seedlings, 2-4 rooted seedlings can be cultured, so number of casuarina branchlet seedlings reaches 2-4 times of original seedling number, scion mother trees for culturing casuarina are reduced, and seedling raising cost is reduced.

The present invention relates to an Aspergillus strain capable of promoting the growth of casuarina root system, which is Aspergillus sp. Casuarina rhizosphere fungus 63, which has been registered and preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on June 28, 2012. The deposit number is CGMCCNo.6311. Casuarina rhizosphere fungus 63 was inoculated into casuarina hydroponic seedlings, and according to the comprehensive evaluation of various indicators of root growth, it was further confirmed that it can promote root growth. Finding functional endophytic fungi that are beneficial to root growth can be applied to Production.

The invention discloses an endophytic fungus capable of improving the activity of casuarina acid phosphatase in a low-phosphorus environment. The endophytic fungus Herpotrichia australis G3 has been registered and preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on November 20, 2019, with the preservation number CGMCC No.18816. The strain is isolated and purified from the root of casuarina, which can significantly increase the acid phosphatase activity of casuarina, thereby enhancing the adaptability of seedlings in a low-phosphorus environment.

The invention discloses a method for establishing an Agrobacterium-mediated short-branch casuarina callus transgenic system. The method comprises: taking short-branch casuarina shoots as explants, inducing callus to obtain callus tissue; selecting callus tissue Put it into the Agrobacterium infection solution for infection culture; co-cultivate the infected callus, and then carry out screening culture; use the light yellow, plump and obviously enlarged resistant calli to screen for adventitious buds Cultivation and rooting culture; finally, judge whether the regenerated seedlings of the casuarina short-branches are transgenic positive plants by GUS staining method, and obtain the transgenic plants of the casuarina short-branches. The present invention adopts specific callus induction medium, co-cultivation medium, resistant callus selection medium and adventitious bud selection medium to construct the Agrobacterium-mediated short-branched Casuarina callus transgenic system, which can not only obtain short The transgenic plants of casuarina twigs can greatly shorten the time of the transformation system and increase the transformation rate.

The invention relates to microbial fertilizer specially used for casuarinas. The invention is characterized in that the invention comprises liquid bacterial fertilizer which is obtained by mixing the fermentation broth of a Bacillus megaterium de Bary (Bacillus megaterium de Bary), a fermentation broth of a Frankia.sp (Frankia.sp) and stable effect promoter in a volume ratio of 1 to 1.5: 1.5 to 2.0: 0.1-0.5. The components of the stable effect promoter are counted by a mass fraction: 0.01 to 0.05 percent of flavonoids, 0.001 to 0.01 percent of coumarin, 0.001 to 0.01 percent of sodium molybdate, 0.001 to 0.01 percent of cobalt sulfate; the rest is water. The stable effect promoter also comprises a zeolite powder and organic fertilizer. The fertilizer can promote the growth of the casuarinas and increase the survival ratio and preservation ratio of the casuarinas as well as increasing the quantity of the casuarinas seedling with a nodule. Therefore, the resisting performance and the drought-resistance ability of the casuarinas are promoted. The crown closure speed of the plantation is increased. The windbreak and sand fixation effect of the casuarinas in the coastal protection forest is strengthened. The strain CCTCC M 207197 of Frankia.sp fgc6 provided by the invention has a better effect.

The invention discloses an endophytic fungus Z1 capable of increasing the biomass of casuarina under a high-salt environment, belonging to the field of microorganisms. The taxonomic name of the endophytic fungus Z1 is: Botrytis ( Botryosphaeria sp. ), was deposited in the General Microorganism Center of China Committee for Culture Collection of Microorganisms on November 20, 2019; the culture preservation number is: CGMCC NO.18817. The bacterial solution of endophytic fungus Z1 can be used to plant casuarina seedlings in high-salt environment by watering rhizosphere soil or direct inoculation of seedlings, which can increase the biomass of casuarina. By establishing the casuarina-endophytic fungal symbiotic system, the growth of casuarina under high-salt stress can be improved, and the data basis and reference can be provided for enriching the species information of endophytic fungi in woody plants and for the production of biological fertilizers in casuarina industrial forests in accordance with.

The invention provides a casuarina equisetifolia gene CeSC20 and an application of the casuarina equisetifolia gene CeSC20. A cDNA sequence of the casuarina equisetifolia gene CeSC20 is shown as SEQ ID NO.1, or is a sequence which is completely complementarily paired with the SEQ ID NO.1, or is a sequence which codes an amino acid sequence shown as SEQ ID NO.2. Functional research is carried out through gene cloning, and the CeSC20 gene can improve the drought resistance of organisms, can be widely applied to improvement of the drought resistance of the organisms, comprises construction of drought-resistant saccharomyces cerevisiae engineering strains, cultivation of drought-resistant plant varieties, improvement of the drought resistance of plants and the like, and has wide application value in the fields of microorganisms and agriculture.

The invention discloses a method for promoting the decomposition of understory litter of tropical Casuarina equisetifolia coastal defense forest, comprising the following steps: A: selecting seeds of A. chinensis, A. japonica and A. japonicus; B. The seeds of sterilization, seed soaking, and seed preparation are carried out; C, the seeds of step B gained a little red, Flos japonica and Toxocarpus are sown under the Casuarina forest, and watered and fertilized in the later stage. The method can promote the decomposition of understory litter in the casuarina coastal protection forest and increase the plant diversity of the understory of casuarina.

The invention discloses a coast oak water planting seedling culturing method. The technology comprises the step of selecting variety and concentration of hormones, medicine liquor soaking time, coast oak budlings and the like for coast oak water planting seedling culturing. Different hormone concentration and soaking time are selected according to different seedling culturing time, so the optimal coast oak root forming number and quality are achieved, the root forming time is shortened, the survival rate of the seedling culturing is improved and the production cost is reduced.

The invention discloses an endophytic fungus capable of improving casuarina phosphorus absorption in a low-phosphorus environment, and the endophytic fungus Z5 belongs to the genus Botrytis ( Botryosphaeria sp. ), which was registered and preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on November 20, 2019, with the preservation number CGMCC No.18813. The strain is isolated and purified from casuarina twigs, which can significantly increase the phosphorus uptake of casuarina, thereby promoting the growth of plants in a low-phosphorus environment.

The invention discloses a method for promoting in-situ decomposition of casuarina leaf litter. The method comprises adding N element to the casuarina leaf litter for 2 to 4 months, and then adding Mn element for 3 to 6 months of decomposition. The method of the present invention fundamentally reduces the litter stock and reduces the risk of forest fires; and the method of the present invention is added under the forest without moving the litter under the forest, and can also promote the return of litter nutrients after accelerated decomposition. Maintaining soil fertility has the advantages of simple operation, ecological environment protection and long-term effect.

The invention relates to the field of under-tree forestation methods, and provides a podocarpus nagi casuarina under-tree forestation method. The defects that in the prior art, a podocarpus nagi plannting method is low in forest space utilization rate and low in yield, and the single creature can survive under the same forest are overcome. The method comprises the following steps of 1 forest selection, 2 forest cleaning and soil preparation, 3 nursery stock selection, 4 planting technology, 5 fertilization, 6 weeding and hole enlarging, 7 pruning, 8 flower and fruit management, 9 pest controland 10 fruit harvesting and storing.

The present invention relates to a formula of a novel Chinese herbal shampoo. The raw materials in the formula are composed of 5-15% of angelica, 1-5% of Angelica dahurica, 1-10% of Schizonepeta and 5-15% of Myrobalan , Clematis 3‑9%, Arborvitae 2‑3%, Eclipta 1‑5%, Morning Glory 11‑19%, Casuarina 5‑7%, Senecio 3‑5%, Morus Alba 3 -5%, Soapberry 1-5%, Glycyrrhizae 5-10%, Potassium Azelayl Diglycinate 1-1.5%, Water-Soluble Hesperidin 4-5%, the novel Chinese medicine plant shampoo of the present invention can effectively Eliminates dandruff and leaves hair radiant and radiant.

The invention relates to an endophytic fungus capable of promoting the growth of casuarina biomass. The endophytic fungus is Aspergillus sp. Casuarina rhizosphere fungus 17, which has been registered and preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on June 28, 2012, and the preservation number is CGMCC No.6309. The casuarina rhizosphere fungus 17 was inoculated into the casuarina hydroponic seedlings, and according to the comprehensive evaluation of various indicators, it was further confirmed that it had a promoting effect on the biomass growth and actually had a synergistic effect on the dry matter of the casuarina. The growth of beneficial functional endophytic fungi can be applied in production.

The invention relates to a horsetail beefwood rooting and seedling raising method.The method comprises the steps that the root of horsetail beefwood is placed in a rooting agent to be soaked and then inserted into a rooting matrix, the rooting matrix is prepared from water and agar with the weight ratio of 1000:8-10, water is added to the surface of the rooting matrix, and rooting culture is carried out on the horsetail beefwood inserted into the rooting matrix.The horsetail beefwood rooting and seedling raising method has the advantages of improving raw material utilization rate exponentially, changing water conveniently, lowering horsetail beefwood rotting and stinking rate and improving the rooting rate.

The invention discloses a preparation method of casuarina tanning and hypoglycemic application of casuarina tanning. The preparation method comprises the steps of alcohol extraction and concentration, ethyl acetate extraction, high-pressure preparative liquid chromatography purification and high-speed counter-current chromatography separation. High-pressure preparative liquid chromatography and high-speed counter-current chromatography are combined, and then technological parameters are optimized, therefore, high-purity casuarina tanning monomer is separated and prepared from a melastoma dodecandrum raw material with complex substance composition. An activity test shows that the casuarina tannin monomer can significantly inhibit the activity of alpha-glucosidase, and can be used for preparing an alpha-glucosidase inhibitor to control the postprandial blood sugar level.

The invention provides a calcium organic fertilizer specially for preventing and treating peanut blight and a preparation method of the calcium organic fertilizer. In the invention, an aerobic high-temperature composting mode is adopted, the raw material formula of the calcium organic fertilizer is as follows: the calcium organic fertilizer comprises the following components by weight percentage:35-45% of pig manure, 8-12% of casuarina, 8-12% of peanut shell, 10-14% of shell powder, 3-6% of calcium superphosphate, 20-30% of water and 0.2-0.5% of effective microorganism (EM) agent. The pig manure is processed by a dry-wet separation technique. By applying regulation and control technologies such as moisture, temperature, pH value, C / N (carbon / nitrogen), C / P (carbon / phosphorus) and the like, based on sprouting index and C / N as maturity evaluation standards, the produced calcium organic fertilizer can meet the national standard of commercial organic fertilizer. In a sand ground peanut blight area along a shore, a special calcium peanut organic fertilizer is used, thereby effectively reducing the peanut blight rate and improving the economic benefits of peanuts.

The invention provides a casuarina equisetifolia gene CeDREB1 and an application of the casuarina equisetifolia gene CeDREB1. The cDNA sequence of the casuarina equisetifolia drought-resistant gene CeDREB1 is shown as SEQ ID NO.1, or is a sequence which is completely complementarily paired with the SEQ ID NO.1, or is a sequence with a coded amino acid sequence shown as SEQ ID NO.2. Functional research is carried out through gene cloning, and the CeDREB1 gene can improve drought resistance of organisms, can be widely applied to improvement of sexual drought resistance of the organisms, comprises construction of drought-resistant saccharomyces cerevisiae engineering strains, cultivation of drought-resistant plant varieties, improvement of drought resistance of plants and the like, and has awide application value in the fields of microorganisms and agriculture.

The invention relates to a series of tannin extract raw materials prepared by ultrafine pulverization and its combination and coupled preparation process and method for a series of tannin extract products. That is to say, the superfine powder materials of oak bowl shell, larch, casuarina, oil orange, black wattle, bayberry, red root, live oak, and acacia bark are subjected to double dynamic extraction or flat transformation Continuous leaching, spiral sedimentation centrifugal separation (horizontal spiral unloading centrifugal separation), foam separation, sedimentation separation, plate and frame filter press, etc. combination, coupling process and equipment processing, correspondingly solve the filtration in the production and processing process of tannin extract raw materials , sol, fine fiber particles, insoluble matter and non-tannin high-level problems, prepared industrial-grade rubber extracts, larch extracts, casuarina extracts, oil orange extracts, and black wattle extracts that meet industry standards , bayberry extract, red root extract, oak tree extract, and acacia extract.

The invention belongs to the technical field of seedlings raising and discloses a method for promoting germination of lateral branches of casuarinas. The method comprises the following steps: utilizing large trees and old trees as material; promoting lateral branches of old trees or big trees of casuarinas to germinate new and small branches. The method comprises the methods of pulling small branches, pressing branches, chopping branches by half and inverting branches. The method is capable of germinating new branches in batch for lateral branches of casuarinas. The number of newly-germinated branches is almost equal to that of small branches originally growing on lateral branches before technical processing. The method for promoting germination of lateral branches of casuarinas has the following beneficial effects: roots easily grow out of new and small branches growing out of lateral branches of casuarinas than original and old branches; rooting rate can be up to more than 80%; and the rooting rate of old and small branches of casuarinas is almost zero.