68 results about "Casuarina equisetifolia" patented technology

The invention discloses a method for promoting recovery of native vegetation in tropical coast sandy land and relates to a method for recovery of native vegetation in non-forested tropical coast sandy wasteland or under the Casuarina equisetifolia forest in sandy land. According to the technical scheme, seeds of proper arbores, shrubs and herbaceous plants are selected to form a seed bank and matched in a proper proportion, branches of flowering shrubs are selected as cutting shoots, an uncovered biodegradable thin carton is planted in the non-forested sand wasteland or under the Casuarina equisetifolia forest in the sand land to serve as a seed bank patch, sandy soil is paved at the bottom in the patch, the seeds and the sandy soil are mixed and spread in the patch, a sunshade net is erected above the seed bank patch in the non-forested sandy wasteland, the forest gap area above the seed bank patch under the Casuarina equisetifolia forest is larger than or equal to 25 square meters, and a sunshade net is erected above the seed bank patch; the cutting shoots of the flowering shrubs are cut and inserted into the place, where the seeds are not germinated or few seedlings emerge, in the seed bank patch. The seed sprouting rate and the seedling survival rate are high, the colonization period of the native vegetation in a completely natural state is shortened, the biological diversity and the structure complexity are improved, and the stability and the stress resistance of a constructed forest community are high.

The invention discloses a rooting method for a casuarina equisetifolia sterile test-tube seedling, which comprises the following steps of: cutting a fresh cut on the base part of the sterile test-tube seedling, inserting the base part of the sterile test-tube seedling into a sterilized and cooled mesenchymal stem cell (MSC) liquid culture medium which contains 20 to 30 mum of naphthylacetic acid and 15 to 25g/L of saccharose and culturing for 2 to 3 days under the conditions of a temperature of 27+/-3 DEG C, an illumination time period of 12 to 20 hours each time and illumination intensity of 45 to 60 mu mol/m<2>/s; taking out the sterile test-tube seedling and washing the base part of the sterile test-tube seedling with sterilized de-ionized water for 2 to 5 times; and transferring the sterile test-tube seedling into the sterilized and cooled MSC liquid culture medium which contains 30 to 50g/L of saccharose for cultivation for 1 to 2 weeks under the condition of a temperature of 27+/-3 DEG C, an illumination time period of 12 to 20 hours each time and illumination intensity of 45 to 60 mu mol/m<2>/s. The rooting method for the casuarina equisetifolia sterile test-tube seedling has the advantages that: rooting is quick and efficient; roots can grow after 10 days of cultivation; rooting efficiency is high and reaches 100 percent after two weeks of cultivation; a large number of roots grow; and the roots extend and grow obviously.

The invention discloses a preparation method and applications of secondary metabolites with the antibacterial and/or antioxidant activity of casuarina equisetifolia endophytic fungi. The method comprises the following steps: (1) separating endophytic fungi from casuarina equisetifolia by adopting a tissue block separating method; (2) fermenting the separated endophytic fungi in a rice solid medium; (3) after fermentation is finished, carrying out cold soaking extraction on the fermentation product by adopting a mixed solution of ethyl acetate and acetone, and carrying out concentration under reduced pressure on the extracted solution, thus obtaining the secondary metabolites of the casuarina equisetifolia endophytic fungi; and (4) screening the secondary metabolites with the antibacterialand/or antioxidant activity by adopting the thin layer chromatography-bioautography method. According to the method, the secondary metabolites with the antibacterial and/or antioxidant activity can beobtained from the casuarina equisetifolia endophytic fungi, and meanwhile, the secondary metabolites capable of resisting pseudomonas solanacearum of casuarina equisetifolia are obtained, so that thebacterial wilt of casuarina equisetifolia can be prevented and treated.

The invention discloses a method for determining and evaluating cold resistance of casuarina equisetifolia and belongs to the technical field of determination and evaluation of cold resistance of tree species. The method is characterized in that: (1) new leaves of field living casuarina equisetifolia are taken as the material and refrigerated for 7-12 hours at the low temperature of minus 2-minus 15 DEG C; and (2) a chlorophyll luminoscope is standardized; a part of determined leaves which are processed are fixed and subjected to dark adaptation for 20-50 minutes; measuring light intensity and gain are adjusted to ensure the initial fluorescence F0 of the leaves which are subjected to dark adaptation to be between 200 and 500; the maximum photochemical amount Fv/Fm of a photosystem II of different parts of the leaves which are subjected to dark adaptation is measured and the measurement is repeated for three times for averaging. The method for determining and evaluating cold resistance of casuarina equisetifolia has the advantages of convenience in operation and low determining cost; and as field living casuarina equisetifolia is determined, specific analysis and evaluation can be performed according to practical condition and space. Therefore, the precision is higher and the casuarina equisetifolia can be determined and evaluated objectively.

The invention relates to an endophytic fungus promoting casuarina equisetifolia root system growth effect. The endophytic fungus is phyllosticta sp. casuarina equisetifolia rhizospheric fungus 15, which is registered and collected in China General Microbiological Culture Collection Center on June 28th, 2012. The collection number is CGMCC No. 6307. The fungus is inoculated in casuarina equisetifolia hydroponic seedling. Comprehensive evaluation is carried out according to various indicators of root system growth, and it is further proved that the casuarina equisetifolia rhizospheric fungus 15 has a promotion effect upon rooting and an actual synergetic effect upon casuarina equisetifolia dry matters. When a functional endophytic fungus beneficial for promoting rooting effect is found, the endophytic fungus can be used in production.

The invention relates to an endophytic fungus promoting casuarina equisetifolia biomass growth. The endophytic fungus is aspergillus sp. casuarina equisetifolia rhizospheric fungus 17, which is registered and collected in China General Microbiological Culture Collection Center on June 28th, 2012. The collection number is CGMCC No. 6309. The casuarina equisetifolia rhizospheric fungus 17 is inoculated in casuarina equisetifolia hydroponic seedling. Comprehensive evaluation is carried out according to various indicators, and it is further proved that the fungus has a promotion effect upon biomass growth and an actual synergetic effect upon casuarina equisetifolia dry matters. When a functional endophytic fungus beneficial for promoting biomass growth is found, the endophytic fungus can be used in production.

The invention provides a calcium organic fertilizer specially for preventing and treating peanut blight and a preparation method of the calcium organic fertilizer. In the invention, an aerobic high-temperature composting mode is adopted, the raw material formula of the calcium organic fertilizer is as follows: the calcium organic fertilizer comprises the following components by weight percentage:35-45% of pig manure, 8-12% of casuarina, 8-12% of peanut shell, 10-14% of shell powder, 3-6% of calcium superphosphate, 20-30% of water and 0.2-0.5% of effective microorganism (EM) agent. The pig manure is processed by a dry-wet separation technique. By applying regulation and control technologies such as moisture, temperature, pH value, C/N (carbon/nitrogen), C/P (carbon/phosphorus) and the like, based on sprouting index and C/N as maturity evaluation standards, the produced calcium organic fertilizer can meet the national standard of commercial organic fertilizer. In a sand ground peanut blight area along a shore, a special calcium peanut organic fertilizer is used, thereby effectively reducing the peanut blight rate and improving the economic benefits of peanuts.

The invention discloses chronic dysentery preparation capable of invigorating spleen and supplementing qi and a preparation method thereof. The chronic dysentery preparation is mainly prepared from the following drug substances in parts by weight: 3-6 parts of coptis chinensis, 10-20 parts of the root of Chinese pulsatilla, 5-15 parts of radices saussureae, 15-25 parts of capitate knotweed herb, 6-12 parts of Java bishopwood root-bark branchlet and leaf, 12-20 parts of astragalus membranaceus, 6-10 parts of griffith streptocaulon root or leaf, 8-15 parts of cassia twig, 18-40 parts of herba portulacae, 6-12 parts of casuarina equisetifolia and 6-15 parts of thyrocarpus sampsonii hance. The chronic dysentery preparation is obvious in curative effect, free of toxic or side effect and short in course of treatment and becomes effective rapidly; chronic dysentery does not relapse when cured by adopting the chronic dysentery preparation.

The invention discloses a constructing and building method for non-suitable-for-forestry new diked tidal flat coast protection forest backbone shelterbelts, and belongs to the technical field of agricultural cultivation. The constructing and building method comprises the following steps: A, establishing basic facilities: digging a main river channel on a new diked tidal flat, building a water control gate, building road traffic of seawall land and a drainage system; B, cutting earth and turning soil, and piling mounds; C, planting container seedlings; D, loosening soil and tending: after a forest survives, performing soil loosening and tending; E, cutting weeds, covering soil and performing maintenance management. The method can make casuarina equisetifolia and other saline-alkali tolerant plants be able to normally grow on a non-suitable-for-forestry coastal new diked tidal flat, and improves survival rate of the protection forest. The method is beneficial for large-area popularization and application, and is an ecological and environmentally-friendly afforestation method. The method has significance for resisting major natural disasters in coastal regions.

The invention discloses an endophytic fungus Y6 for enhancing the oxidation resistance of Casuarina equisetifolia in a high-salt environment, belonging to the technical field of microorganisms. The endophytic fungus Y6 is classified and named as Hypoxylon sp., and is preserved in China General Microbiological Culture Collection Center on November 20, 2019, with an accession number being CGMCC NO.18814. The bacterial liquid of the endophytic fungus Y6 is applied to planting of Casuarina equisetifolia seedlings in a high-salt environment in a rhizosphere soil watering mode or a direct seedlinginoculation mode, and can improve the oxidation resistance of Casuarina equisetifolia.

The invention discloses a method for promoting in-situ decomposition of casuarina equisetifolia leaf litters. The method comprises the following steps: adding an N element into the casuarina equisetifolia leaf litters, after 2-4 months, adding an Mn element, and conducting decomposition for 3-6 months. According to the method disclosed by the invention, the litter stock is fundamentally reduced, and the forest fire occurrence risk is reduced; and moreover, according to the method, the elements are added under the forest, the litters under the forest are not moved, regression of nutrients of the litters can be promoted after decomposition is accelerated, soil fertility is maintained, and the method has the advantages of being easy to operate, ecological, environmentally friendly and long in effect.

The invention relates to a rejuvenation method of short-branch casuarina equisetifolia mature woods. The method comprises the steps that short-branch casuarina equisetifolia branches are cut from theshort-branch casuarina equisetifolia mature woods, tender branchlets on the branches are cut off, and the branches serve as scion branches; short-branch casuarina equisetifolia seedlings serve as stocks, the scion branches are grafted to the stocks through a cut-grafting method or a cut-attachment grafting method, then the scion branches and the stocks are bound through sealing films, and the scion branches are wrapped; and the sealing films wrapping the scion branches are coated with wax layers. According to the rejuvenation method of the short-branch casuarina equisetifolia mature woods, excellent germplasm resource rejuvenation is achieved through the cut-attachment grafting method or the cut-grafting method, sealing is conducted in a sealing film wrapping and waxing mode, connector moisture losses can be reduced, rapid formation of calluses is promoted, outside influences such as morning fog and insects and ants can be effectively avoided, the rejuvenation survival rate of mature short-branch casuarina equisetifolia trees is increased to 70% or above, adopted wax can be common household candles or industrial paraffin, the cost is low, and operation is easy and fast.

The invention relates to an endophytic fungus promoting casuarina equisetifolia photosynthesis. The endophytic fungus is aspergillussp. casuarina equisetifolia rhizospheric fungus 7, which is registered and collected in China General Microbiological Culture Collection Center on June 28th, 2012. The collection number is CGMCC No. 6305. The casuarina equisetifolia rhizospheric fungus 7 is inoculated in casuarina equisetifolia hydroponic seedling. Comprehensive evaluation is carried out according to various indicators of photosynthesis, and it is further proved that the fungus has a promotion effect upon photosynthesis. When a functional endophytic fungus beneficial for promoting photosynthesis is found, the endophytic fungus can be used in production. Through a fungus solution watering method, the endophytic fungus is inoculated on casuarina equisetifolia seedlings. The endophytic fungus has substantial effects in promoting plant photosynthesis and increasing casuarina equisetifolia dry matter accumulation.

The invention discloses a method for establishing an agrobacterium tumefaciens-mediated casuarina equisetifolia callus transgenic system, which comprises the following steps: taking a casuarina equisetifolia branch as an explant, and inducing a callus to obtain a callus; selecting and putting the callus into agrobacterium infection liquid for infection culture; carrying out co-culture on the infected calluses, and then carrying out screening culture; performing adventitious bud screening culture and rooting culture on the faint yellow, plump and obviously expanded resistant calluses; and finally, judging whether the Casuarina equisetifolia regenerated seedling is a transgenic positive plant or not through a GUS staining method to obtain a Casuarina equisetifolia transgenic plant. The specific callus induction culture medium, the co-culture culture medium, the resistant callus screening culture medium and the adventitious bud screening culture medium are adopted to construct the agrobacterium tumefaciens-mediated casuarina equisetifolia callus transgenic system, so that not only can the transgenic plant of the casuarina equisetifolia be obtained, but also the time of transforming the system can be greatly shortened; and the conversion rate is improved.

The invention provides a high-yield biological fertilizer of sagittaria sagittifolia and a making method thereof and belongs to the field of agricultural planting. The biological fertilizer is prepared by mixing and fermenting the following raw materials in parts by weight: 50-55 parts of pea shells, 10-15 parts of egg shells, 15-20 parts of pomegranate peels, 30-35 parts of straws, 8-10 parts ofshrimp shells, 30-35 parts of duck manures, 25-30 parts of cow manures, 0.4-0.6 part of a casuarina equisetifolia extract, 0.1-0.3 part of a carduus marianus extract, 0.02-0.03 part of a compound bacterial agent and 0.002-0.004 part of an additive. The high-yield biological fertilizer and the making method thereof provided by the invention have the benefits that the survival rate of sagittaria sagittifolia seedlings can be improved, and diseases are prevented, so that the yield and the bulb nutrition value are improved; the fertilizer can be saved, is suitable for large-scale systematic planting and increases the yield and the income for growers.

The invention discloses organic fertilizer capable of preventing and treating grape root maggots. The organic fertilizer is prepared by, by weight, 3.5-3.8 parts of Omphalia lapidescens Schroet. sclerotia, 1.4-1.6 parts of magnesium sulfate, 8-10 parts of potassium dihydrogen phosphate, 7-9 parts of casuarina equisetifolia, 6-7 parts of attapulgite, 2.2-2.3 parts of acetylsalicylic acid, 20-24 parts of pig manure, 15-18 parts of nano carbon black, 55-57 parts of domestic organic waste, 16-17 parts of swine bone discarded by a slaughter house, 12-13 parts of bovine bone discarded by a slaughter house, 2.8-3.4 parts of citric acid and an appropriate amount of water. The organic fertilizer has the advantages that aiming at the nutrition requirements of grape, the potassium dihydrogen phosphate, the pig manure and the casuarina equisetifolia are added into the organic fertilizer to achieve comprehensive nutrition supplement, an anaerobic-aerobic-mixed fermentation system guarantees the sufficient decomposition of fertilizer particles, incubation and survival of root maggot eggs are avoided, the activities of the root maggots can be inhibited greatly by the added Omphalia lapidescens Schroet. sclerotia, grape root system health is guaranteed, the raw materials of the organic fertilizer are harmless to soil environments, and the organic fertilizer is high in economic and environmental benefits.

The invention discloses endophytic fungus strain capable of enhancing activity of acid phosphatase of casuarina equisetifolia in low-phosphorus-content environment. The endophytic fungus strain is named Herpotrichia australis G3, and is registered and preserved in China General Microbiological Culture Collection Center since November 20, 2019 under the preservation number of CGMCC No. 18816. The endophytic fungus strain is separated and purified from the root of the casuarina equisetifolia, and is capable of significantly improving the activity of the acid phosphatase of the casuarina equisetifolia so as to enhance adaptability of seedlings in the low-phosphorus-content environment.

The invention discloses an endophytic fungus Z1 for increasing the biomass of Casuarina equisetifolia in a high-salt environment, and belongs to the field of microorganisms. The classification name ofthe endophytic fungus Z1 is Botryosphaeria sp., and the endophytic fungus Z1 is preserved in China General Microbiological Culture Collection Center on November 20, 2019 with the preservation numberof CGMCC NO.18817. The bacterial liquid of the endophytic fungus Z1 is applied to planting of Casuarina equisetifolia seedlings in the high-salt environment in a rhizosphere soil watering mode or a seedling direct inoculation mode, and the biomoass of the Casuarina equisetifolia can be increased. A Casuarina equisetifolia-endophytic fungus symbiotic system is built to improve the growth of Casuarina equisetifolia under high-salt stress and provide a data basis and a reference basis for enriching strain information of endophytic fungi of woody plants and manufacturing biological bacterial fertilizers for Casuarina equisetifolia industrial forest operation.

The invention provides a root-divided alternative irrigation method with a sepiolite salt partitioning layer. The root-divided alternative irrigation method includes the steps of water pipe laying, and further includes the steps of salt partitioning layer laying; the salt partitioning layer sequentially comprises a leak-proof layer, a rough stone layer, a fine stone layer and a nutrition layer from bottom to top. The invention further provides an application of the root-divided alternative irrigation method with the sepiolite salt partitioning layer to planting casuarina equisetifolia in saline-alkali land. The root-divided alternative irrigation method is adopted, the adaptive capacity of plant roots is also greatly enhanced, and the growth vigor in soil with the water content of 10.0% to 11.0% is good; meanwhile, the plant roots are good in development and high in growth speed; compared with the common fresh water irrigation amount, the per-mu fresh water irrigation amount is reduced, and the saved fresh water amount reaches 60% to 70%; the content of the medicinal components in plants is increased, and the medicinal value of the plants is greatly improved.

The invention provides a casuarina equisetifolia gene CeDREB1 and an application of the casuarina equisetifolia gene CeDREB1. The cDNA sequence of the casuarina equisetifolia drought-resistant gene CeDREB1 is shown as SEQ ID NO.1, or is a sequence which is completely complementarily paired with the SEQ ID NO.1, or is a sequence with a coded amino acid sequence shown as SEQ ID NO.2. Functional research is carried out through gene cloning, and the CeDREB1 gene can improve drought resistance of organisms, can be widely applied to improvement of sexual drought resistance of the organisms, comprises construction of drought-resistant saccharomyces cerevisiae engineering strains, cultivation of drought-resistant plant varieties, improvement of drought resistance of plants and the like, and has awide application value in the fields of microorganisms and agriculture.

The invention discloses an endophytic fungus J12 for promoting casuarina equisetifolia growth in a low-phosphorus environment, which belongs to the technical field of microorganisms. The endophytic fungus J12 is classified and named as Pseudofusicoccum violaceum, is registered and preserved in the China General Microbiological Culture Collection Center on November 20, 2019, and has a preservationnumber of CGMCC NO. 18815. The strain is separated and purified from the stem of casuarina equisetifolia, and can significantly promote the increase of the seedling height and ground diameter of casuarina equisetifolia.

The invention relates to the preparation technology of rhizoma gastrodiae vinegar, in particular to a preparation method of rhizoma gastrodiae vinegar, and solves the problem that existing rhizoma gastrodiae vinegar cannot bring the medicinal value of rhizoma gastrodiae into full play. The preparation method includes the steps of firstly, mixing clean rhizoma gastrodiae, eggplant roots, Drynaria fortunei, rhizoma chuanxiong, Casuarina equisetifolia, and soaking into a sodium bicarbonate solution of 60 DEG C for 40 minutes; secondly, adding sucrose, starch and distiller's yeast into a second mixture, evenly stirring, and performing sealed fermentation for 3 days under 30 DEG C to obtain a third mixture, wherein the mass ratio of the second mixture to the sucrose to the starch to the distiller's yeast is 10:1:3:1; adding purified water and vinegar koji into the third mixture, and then fermenting in a liquid fermentation tank under 26-29 DEG C for 6-8 days to obtain a fourth mixture, wherein the mass ratio of the third mixture to the purified water and the vinegar koji is 5:15:1. The preparation method is applicable to the preparation of rhizoma gastrodiae vinegar.