Method for determining and evaluating cold resistance of casuarina equisetifolia

Abstract

The invention discloses a method for determining and evaluating cold resistance of casuarina equisetifolia and belongs to the technical field of determination and evaluation of cold resistance of tree species. The method is characterized in that: (1) new leaves of field living casuarina equisetifolia are taken as the material and refrigerated for 7-12 hours at the low temperature of minus 2-minus 15 DEG C; and (2) a chlorophyll luminoscope is standardized; a part of determined leaves which are processed are fixed and subjected to dark adaptation for 20-50 minutes; measuring light intensity and gain are adjusted to ensure the initial fluorescence F0 of the leaves which are subjected to dark adaptation to be between 200 and 500; the maximum photochemical amount Fv / Fm of a photosystem II of different parts of the leaves which are subjected to dark adaptation is measured and the measurement is repeated for three times for averaging. The method for determining and evaluating cold resistance of casuarina equisetifolia has the advantages of convenience in operation and low determining cost; and as field living casuarina equisetifolia is determined, specific analysis and evaluation can be performed according to practical condition and space. Therefore, the precision is higher and the casuarina equisetifolia can be determined and evaluated objectively.

Description

technical field [0001] The invention belongs to the technical field of identification and evaluation of cold resistance of tree species, in particular to a method for identification and evaluation of cold resistance of casuarina. Background technique [0002] Casuarina, native to Australia and the Pacific Islands, has been introduced to China for more than 80 years. It is cultivated in Guangdong, Guangxi, Fujian, Taiwan and South China Sea Islands. The material is strong and heavy, and is used for construction, furniture, and papermaking; the bark can be used to extract tannins and dyes; the branches and leaves are livestock feed, and the seeds are used to feed poultry; the crown is tower-shaped, elegant, and it is a tree species for garden greening. [0003] Casuarinas play an important role in preventing typhoon damage, preventing wave erosion, sand fixation, restoring coastal terrestrial ecosystems, and improving soil in barren coastal sandy land and severely degraded so...

AI Technical Summary

Problems solved by technology

Since the 21st century, Zhejiang Zhoushan and Ningbo areas have introduced species from Taizhou. Due to the low temperature (-5~-6 °C) freezing weather in Hangzhou Bay area in some years, the introduced casuarinas often suffer from severe freezing damage, which has caused serious damage to the construction of coastal shelter forests. Big loss

The traditional method evaluates the cold resistance of seedlings by observing the dead state of the branches and leaves after low temperature. This method is inconvenient to operate, has low accuracy and high identification cost.