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Rooting method of casuarina equisetifolia sterile test-tube seedling

A test-tube seedling, casuarina japonica technology is applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of long time for inducing rooting, wound callus, low rooting rate, etc., and achieves root elongation and growth. Obvious, broad development prospects, easy to promote the effect

Inactive Publication Date: 2011-01-26
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For sterile test-tube seedlings of casuarina, it is impossible to induce rooting through traditional hydroponics, because traditional casuarina hydroponics requires sufficient sunlight and continuous and frequent replacement of culture medium, which seriously restricts the traditional casuarina hydroponics. Rooting method is suitable for rooting culture of aseptic test-tube plantlets
Because there is no direct sunlight, and the sterile test tube seedlings are young (not lignified), and the frequent replacement of the culture medium is also likely to cause pollution, etc.
[0006] Therefore, someone proposed to take the aseptic test-tube plantlets directly to the greenhouse for hardening without roots, and then use the traditional hydroponics method to cultivate and take root. The test found that the rooting rate of this method is very low, and the aseptic test-tube plantlets are easy to grow without roots. dead before
There are also researchers (Duhoux et al., 1990; Parthiban et al., 1996) to induce rooting of aseptic test-tube plantlets in solid medium supplemented with substances such as hormones and activated carbon, but the rooting rate is not high, the time for inducing rooting is longer, and it is easy to induce Callus produced from base wound of sterile test-tube plantlets
In addition, some researchers have replaced the solid medium usually used for Casuarina equisetifolia tissue culture with liquid medium to induce rooting culture of sterile test-tube plantlets. The rooting rate can reach about 50%, but the elongation and growth of roots are inhibited. The growth is slow, and the wound will also produce callus, and the effect is also unsatisfactory

Method used

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  • Rooting method of casuarina equisetifolia sterile test-tube seedling

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A rooting method of casuarina aseptic test-tube seedlings, the steps are as follows:

[0022] (1) MSC liquid medium (improved medium containing 1 / 2MS medium macroelements and 1 / 1MS medium trace elements, and vitamins of NN medium) containing 25μM naphthaleneacetic acid and 20g / L sucrose was prepared, with 0.1 Adjust the pH to 5.6 with M KOH.

[0023] The composition of MSC liquid medium is: NH 4 NO 3 825mg / L, KNO 3 950mg / L, CaCl 2 2H 2 O220mg / L, MgSO 4 ·7H 2 O 185mg / L, KH 2 PO 4 85mg / L, KI 0.83mg / L, H 3 BO 3 6.2mg / L, MnSO 4 4H 2 O 22.3mg / L, ZnSO 4 ·7H 2 O 8.6mg / L, NaMoO 4 2H 2 O 0.25mg / L, CoCl 2 ·6H 2 O 0.025mg / L, CuSO 4 ·5H 2 O 0.025mg / L, Na 2 FeEDTA (FeSO 4 ·7H 2 O27.8mg / L, Na 2 EDTA·2H 2 O 37.3mg / L), biotin 0.05mg / L, folic acid 5mg / L, glycine 2mg / L, inositol 100mg / L, niacin 5mg / L, vitamin B6 0.05mg / L, vitamin B1 0.05mg / L.

[0024] (2) Put filter paper and cotton as a support at the bottom of the test tube (25×200mm), fill in 30ml of the ...

Embodiment 2

[0031] Except containing 20 μ M naphthaleneacetic acid and 15g / L sucrose in the MSC liquid medium in step (1), except containing 35g / L sucrose in the MSC liquid medium of step (6), other operations are all identical with embodiment 1.

[0032] Adopt the condition of embodiment 2, done repeated test 5 times, each repeats and adopts 6 strains of sterile test-tube plantlets to test, the data after rooting is carried out statistical analysis, rooting rate is 92.5%, the average rooting number 1.72 of rooting seedlings, The average longest root length of rooted seedlings was 1.63 cm.

Embodiment 3

[0034] Except containing 30 μ M naphthaleneacetic acid and 25g / L sucrose in the MSC liquid medium in step (1), except containing 48g / L sucrose in the MSC liquid medium of step (6), other operations are all identical with embodiment 1.

[0035]Adopt the condition of embodiment 2, done repeated test 5 times, each repeats and adopts 6 strains of sterile test-tube plantlets to test, the data after rooting is carried out statistical analysis, rooting rate is 86.0%, the average rooting number 1.35 of rooting seedlings, The average longest root length of rooted seedlings was 3.12 cm.

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Abstract

The invention discloses a rooting method for a casuarina equisetifolia sterile test-tube seedling, which comprises the following steps of: cutting a fresh cut on the base part of the sterile test-tube seedling, inserting the base part of the sterile test-tube seedling into a sterilized and cooled mesenchymal stem cell (MSC) liquid culture medium which contains 20 to 30 mum of naphthylacetic acid and 15 to 25g / L of saccharose and culturing for 2 to 3 days under the conditions of a temperature of 27+ / -3 DEG C, an illumination time period of 12 to 20 hours each time and illumination intensity of 45 to 60 mu mol / m<2> / s; taking out the sterile test-tube seedling and washing the base part of the sterile test-tube seedling with sterilized de-ionized water for 2 to 5 times; and transferring the sterile test-tube seedling into the sterilized and cooled MSC liquid culture medium which contains 30 to 50g / L of saccharose for cultivation for 1 to 2 weeks under the condition of a temperature of 27+ / -3 DEG C, an illumination time period of 12 to 20 hours each time and illumination intensity of 45 to 60 mu mol / m<2> / s. The rooting method for the casuarina equisetifolia sterile test-tube seedling has the advantages that: rooting is quick and efficient; roots can grow after 10 days of cultivation; rooting efficiency is high and reaches 100 percent after two weeks of cultivation; a large number of roots grow; and the roots extend and grow obviously.

Description

technical field [0001] The invention belongs to the technical field of plant asexual propagation, in particular, the invention relates to a method for rooting casuarina aseptic test-tube seedlings. Background technique [0002] Casuarina is a tree species with important ecological, social and economic benefits, and the research and development of this tree species has global impact. At present, there are about 2 million hectares of casuarina plantations planted in tropical and subtropical regions of the world. Casuarina is the main tree species for building coastal shelterbelts, and it is also a pioneer tree species for farmland shelterbelts and restoration of degraded land vegetation. Its wood can be used to produce pillars, fuel wood, boards and pulp. At present, my country's casuarina plantation area has reached more than 300,000 hectares. Casuarinas play an irreplaceable role in windbreak, sand fixation and vegetation restoration in the coastal areas of South China; th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 仲崇禄姜清彬张勇曾炳山陈羽陈珍
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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