Preparation method and applications of secondary metabolites with antibacterial and/or antioxidant activity of casuarina equisetifolia endophytic fungi

A technology of secondary metabolites and antioxidant activity, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc.

Inactive Publication Date: 2019-01-29
SOUTH CHINA AGRI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of preparation method and application of secondary metabolites of Casuarina endophytic fungus antibacterial and / or antioxidative activity, which solves the problem that there is currently no secondary metabolites of Casuarina endophytic fungi. The research and the problem of the control of casuarina bacterial wilt can obtain secondary metabolites with antibacterial and / or antioxidant activity from casuarina endophytes, and obtain secondary metabolites with resistance to casuarina bacterial wilt. Metabolites for the control of casuarina bacterial wilt

Method used

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  • Preparation method and applications of secondary metabolites with antibacterial and/or antioxidant activity of casuarina equisetifolia endophytic fungi
  • Preparation method and applications of secondary metabolites with antibacterial and/or antioxidant activity of casuarina equisetifolia endophytic fungi
  • Preparation method and applications of secondary metabolites with antibacterial and/or antioxidant activity of casuarina equisetifolia endophytic fungi

Examples

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experiment example 1

[0026] Experimental example 1 Isolation and identification of endophytic fungi in casuarina

[0027] (1) Isolation of endophytic fungi from casuarina

[0028] Wash casuarina branches and fruits with clean water, treat with 70% ethanol for 30 seconds, then treat with 0.2% mercuric chloride for 20 minutes, and finally rinse with sterile water for 3 times, each time for 5 minutes, and place them on sterile filter paper to dry . After removing the epidermis, the branches were divided into pieces of about 0.5 cm × 0.5 cm × 0.5 cm in size, and placed on a PDA medium plate (containing 500 μg / mL streptomycin sulfate, streptomycin sulfate was purchased from Sigma, USA, 99 %), 3 pieces / dish, cultured in the dark at 28°C in a constant temperature incubator, after the endophytic fungus grows, pick a small section of mycelia from the edge of each colony and inoculate it on the PDA medium, and purify continuously for many times , until the colony shape is consistent. Store at 4°C for lat...

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Abstract

The invention discloses a preparation method and applications of secondary metabolites with the antibacterial and/or antioxidant activity of casuarina equisetifolia endophytic fungi. The method comprises the following steps: (1) separating endophytic fungi from casuarina equisetifolia by adopting a tissue block separating method; (2) fermenting the separated endophytic fungi in a rice solid medium; (3) after fermentation is finished, carrying out cold soaking extraction on the fermentation product by adopting a mixed solution of ethyl acetate and acetone, and carrying out concentration under reduced pressure on the extracted solution, thus obtaining the secondary metabolites of the casuarina equisetifolia endophytic fungi; and (4) screening the secondary metabolites with the antibacterialand/or antioxidant activity by adopting the thin layer chromatography-bioautography method. According to the method, the secondary metabolites with the antibacterial and/or antioxidant activity can beobtained from the casuarina equisetifolia endophytic fungi, and meanwhile, the secondary metabolites capable of resisting pseudomonas solanacearum of casuarina equisetifolia are obtained, so that thebacterial wilt of casuarina equisetifolia can be prevented and treated.

Description

technical field [0001] The invention relates to a secondary metabolite of an endophytic fungus, in particular to a preparation method and application of a secondary metabolite of the endophytic fungus casuarina with antibacterial and / or antioxidant activity. Background technique [0002] Casuarina spp., belonging to Casuarinaceae (Casuarinaceae), evergreen trees or shrubs, native to Australia, Pacific Islands and southeastern Asia. Casuarina is widely used in the construction of coastal shelterbelts in tropical and subtropical regions due to its special functions such as rapid growth, drought resistance, windbreak and sand fixation, salt and alkali tolerance, and biological nitrogen fixation. Economic forest and landscaping tree species. [0003] In recent years, due to the single species of casuarinas along the coast, the lack of disease-resistant varieties and the influence of natural disasters and other factors, the occurrence of casuarina bacterial wilt has become incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A01N63/04A01P1/00C12R1/645
CPCC12P1/02A01N63/30C12R2001/645C12N1/145
Inventor 单体江王军毛子翎王胜坤伍慧雄谢银燕吴春银
Owner SOUTH CHINA AGRI UNIV
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