Endophytic fungus J12 for promoting growth of casuarina equisetifolia in low-phosphorus environment

A technology of endophytic fungi and casuarina, which is applied in the field of microorganisms, can solve the problems of phosphorus deficiency in rhizosphere soil and low phosphorus mobility, and achieve the effect of promoting seedling height

Active Publication Date: 2020-04-07
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus has little mobility in the soil, and plants usually absorb the soil phosphorus on the surface of the rhizosphere to supple

Method used

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  • Endophytic fungus J12 for promoting growth of casuarina equisetifolia in low-phosphorus environment
  • Endophytic fungus J12 for promoting growth of casuarina equisetifolia in low-phosphorus environment
  • Endophytic fungus J12 for promoting growth of casuarina equisetifolia in low-phosphorus environment

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Experimental program
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example 1

[0017] Example 1 Isolation of endophytic fungi from casuarina

[0018] 1. Main equipment

[0019] Ultra-clean workbench SW-CJ-1FD, constant temperature incubator HH•B11-II, constant temperature culture oscillator zhwy-211B, ten-thousandth balance AR1140, automatic vertical sterilizer LMQ.C-4060, ultrapure water Machine P60-CW etc.

[0020] 2. Main Reagents and Media

[0021] Reagents: 15% sodium hypochlorite, 75% absolute ethanol, primer PAGE 11-59bp OD 1-2, DNA electrophoresis loading buffer, GoodViewTM nucleic acid dye, 2×Tap PCR MasterMix, fungal DNA extraction kit, DNA purification and recovery kit.

[0022] Medium: (1) Modified Martin agar medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C 6 h 12 o 6 •H 2 O) 20.0g, Potassium dihydrogen phosphate (KH 2 PO 4 ) 1.0 g, magnesium sulfate (MgSO 4 •7H 2 O) 0.5g, agar 15.0g, ultrapure water 1000ml, pH 6.2~6.6.

[0023] (2) Improved Martin's liquid medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C 6 ...

Embodiment 2

[0042] Bacterial solution preparation: The screened endophytic fungi (J12) were cultured in potato dextrose agar medium at 28°C for one week, then inserted into 60 mL potato dextrose liquid medium, and cultured in a constant temperature shaker for 72 h (28°C, 160 r min -1 ), and then diluted with 40 mL of sterile water, the concentration of the diluted bacterial solution was 8.75×10 5 cfu / mL, this concentration and dose were used for inoculation in subsequent experiments.

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Abstract

The invention discloses an endophytic fungus J12 for promoting casuarina equisetifolia growth in a low-phosphorus environment, which belongs to the technical field of microorganisms. The endophytic fungus J12 is classified and named as Pseudofusicoccum violaceum, is registered and preserved in the China General Microbiological Culture Collection Center on November 20, 2019, and has a preservationnumber of CGMCC NO. 18815. The strain is separated and purified from the stem of casuarina equisetifolia, and can significantly promote the increase of the seedling height and ground diameter of casuarina equisetifolia.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an endophytic fungus J12 which can promote the growth of casuarina under low-phosphorus environment. Background technique [0002] Phosphorus participates in the construction of macromolecular substances in plant cells, photosynthetic metabolism, and has the effect of improving plant stress resistance. It is one of the essential nutrients in the process of plant growth and development. Existing studies have shown that phosphorus deficiency exists in the soil that accounts for 74% of the cultivated land in my country, and phosphorus deficiency has become a major factor limiting plant productivity in my country. In the southern coastal sandy land, because most of the phosphorus in the soil is easily combined with calcium and iron ions to form insoluble phosphate, the phenomenon of soil phosphorus deficiency is more serious. However, it has been reported that in ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G7/06C12R1/645
CPCA01G7/06C12N1/145C12R2001/645
Inventor 李键梁安洁林勇明洪滔范海兰
Owner FUJIAN AGRI & FORESTRY UNIV
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