Endophytic fungus Y6 for enhancing oxidation resistance of Casuarina equisetifolia in high-salt environment

A technology of anti-oxidation ability and endophytic fungi, applied in the field of microorganisms, can solve the problems of less research on endophytic fungi in woody plants, and achieve the effect of alleviating damage

Active Publication Date: 2020-04-21
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A series of previous studies have shown that the symbiont between plant endophytic fungi and the host is beneficial for the host to cope with the adverse environment and promote the growth and development of the plant. Less research on endophytic fungi

Method used

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  • Endophytic fungus Y6 for enhancing oxidation resistance of Casuarina equisetifolia in high-salt environment
  • Endophytic fungus Y6 for enhancing oxidation resistance of Casuarina equisetifolia in high-salt environment
  • Endophytic fungus Y6 for enhancing oxidation resistance of Casuarina equisetifolia in high-salt environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation of Schima superba endophytic fungi

[0021] 1. Main equipment

[0022] AX224ZH electronic balance (OHAUS), LS-75HD high-pressure steam sterilizer (Jinan Noble Medical Equipment Co., Ltd.), JB-CJ-1500FX ultra-clean workbench (Suzhou Jiabao Purification Engineering Equipment Co., Ltd.), DNP-9162 constant temperature culture Box (Shanghai Jinghong), ZHWY2111B constant temperature shaking incubator (Shanghai Zhicheng), Beckman ultracentrifuge (USA), etc. Main instruments for strain identification: FQD-48A PCR amplification instrument (BIOER), EPS-100 nucleic acid electrophoresis instrument (Shanghai Tianneng Technology), SeqStudio DNA sequencer (USA), mortar, ice pack, foam box, pipette (Thermo), 1.5ml and 2ml EP tubes (Axygen).

[0023] 2. Main reagents and media

[0024] Reagents: 70% alcohol, sodium hypochlorite, potato dextrose agar (PDA) medium, potato dextrose (PDB) medium, NaCl, Taq enzyme and PCR related reagents, primers (ITS1 / ITS4), OMEGA fungal geno...

Embodiment 2

[0042] Bacterial solution preparation: Inoculate the activated salt-tolerant strains in 60mL Potato Dextrose Medium (PDB) liquid medium, and place them on a constant temperature shaker for 72h (28℃, 160 r•min-1). Calculate the concentration of the bacterial solution by hemocytometer, and the concentration is 8.75×10 5 cfu / mL bacterial solution.

[0043] In this experiment, a soil culture pot experiment was used. The selected Casuarina equisetifolia was an annual seedling with an average seedling height of 20cm and an average ground diameter of 3.0mm, provided by the Chihu State-owned Forest Farm in Hui'an, Fujian Province. On July 3, 2017, Casuarina equisetifolia was selected and planted in a plastic basin with a diameter of 15 cm and a height of 10 cm. The soil required for the experiment was mixed and weighed, and the same amount (4kg) of yellow heart soil was placed in each pot. The nutrient content of the soil is shown in Table 1. After a month of restorative growth, the in...

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Abstract

The invention discloses an endophytic fungus Y6 for enhancing the oxidation resistance of Casuarina equisetifolia in a high-salt environment, belonging to the technical field of microorganisms. The endophytic fungus Y6 is classified and named as Hypoxylon sp., and is preserved in China General Microbiological Culture Collection Center on November 20, 2019, with an accession number being CGMCC NO.18814. The bacterial liquid of the endophytic fungus Y6 is applied to planting of Casuarina equisetifolia seedlings in a high-salt environment in a rhizosphere soil watering mode or a direct seedlinginoculation mode, and can improve the oxidation resistance of Casuarina equisetifolia.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to an endophytic fungus Y6 that can increase the antioxidant capacity of Casuarina equisetifolia in a high-salt environment. Background technique [0002] Salt stress seriously affects the growth and development of crops and woodland plants, and is a major abiotic stress worldwide. Excessive salt separators, such as Na + , K + , Cl - After being ingested by plants, the ion concentration in plant cells will increase, and various biological enzymes in plant cells can only be active in a narrow range of ion concentrations, such as Na + Ions and Cl - The ion requirement is less than 50mM, for K + For ions, it is 0.1-0.2M. Therefore, changes in ion concentration affect the activity of biological enzymes, thereby affecting plant growth. Salt stress can also impair the function of plant cell membranes by affecting a series of changes in the composition, permeability, and tran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00C12R1/645
CPCC12N1/145C12R2001/645
Inventor 李键龙凤林勇明洪滔陈灿
Owner FUJIAN AGRI & FORESTRY UNIV
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