Endophytic fungus Y6 for enhancing oxidation resistance of Casuarina equisetifolia in high-salt environment
A technology of anti-oxidation ability and endophytic fungi, applied in the field of microorganisms, can solve the problems of less research on endophytic fungi in woody plants, and achieve the effect of alleviating damage
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Embodiment 1
[0020] Example 1 Isolation of Schima superba endophytic fungi
[0021] 1. Main equipment
[0022] AX224ZH electronic balance (OHAUS), LS-75HD high-pressure steam sterilizer (Jinan Noble Medical Equipment Co., Ltd.), JB-CJ-1500FX ultra-clean workbench (Suzhou Jiabao Purification Engineering Equipment Co., Ltd.), DNP-9162 constant temperature culture Box (Shanghai Jinghong), ZHWY2111B constant temperature shaking incubator (Shanghai Zhicheng), Beckman ultracentrifuge (USA), etc. Main instruments for strain identification: FQD-48A PCR amplification instrument (BIOER), EPS-100 nucleic acid electrophoresis instrument (Shanghai Tianneng Technology), SeqStudio DNA sequencer (USA), mortar, ice pack, foam box, pipette (Thermo), 1.5ml and 2ml EP tubes (Axygen).
[0023] 2. Main reagents and media
[0024] Reagents: 70% alcohol, sodium hypochlorite, potato dextrose agar (PDA) medium, potato dextrose (PDB) medium, NaCl, Taq enzyme and PCR related reagents, primers (ITS1 / ITS4), OMEGA fungal geno...
Embodiment 2
[0042] Bacterial solution preparation: Inoculate the activated salt-tolerant strains in 60mL Potato Dextrose Medium (PDB) liquid medium, and place them on a constant temperature shaker for 72h (28℃, 160 r•min-1). Calculate the concentration of the bacterial solution by hemocytometer, and the concentration is 8.75×10 5 cfu / mL bacterial solution.
[0043] In this experiment, a soil culture pot experiment was used. The selected Casuarina equisetifolia was an annual seedling with an average seedling height of 20cm and an average ground diameter of 3.0mm, provided by the Chihu State-owned Forest Farm in Hui'an, Fujian Province. On July 3, 2017, Casuarina equisetifolia was selected and planted in a plastic basin with a diameter of 15 cm and a height of 10 cm. The soil required for the experiment was mixed and weighed, and the same amount (4kg) of yellow heart soil was placed in each pot. The nutrient content of the soil is shown in Table 1. After a month of restorative growth, the in...
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