An endophytic fungus y6 that enhances the antioxidant capacity of casuarina under high-salt environment
A technology of anti-oxidation ability and endophytic fungi, applied in the field of microorganisms, can solve the problems of less research on endophytic fungi in woody plants, and achieve the effect of alleviating damage
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Embodiment 1
[0020] Example 1 Isolation of endophytic fungi of Schima superba
[0021] 1. Main equipment
[0022] AX224ZH electronic balance (OHAUS), LS-75HD high-pressure steam sterilizer (Jinan Laibao Medical Equipment Co., Ltd.), JB-CJ-1500FX ultra-clean workbench (Suzhou Jiabao Purification Engineering Equipment Co., Ltd.), DNP-9162 constant temperature culture box (Shanghai Jinghong), ZHWY2111B constant temperature shaking incubator (Shanghai Zhicheng), Beckman ultracentrifuge (USA), etc. Main instruments for strain identification: FQD-48APCR amplification instrument (BIOER), EPS-100 nucleic acid electrophoresis instrument (Shanghai Tianneng Technology), SeqStudio DNA sequencer (USA), mortar, ice pack, foam box, pipette (Thermo), 1.5ml and 2ml EP tubes (Axygen).
[0023] 2. Main Reagents and Media
[0024] Reagents: 70% alcohol, sodium hypochlorite, potato dextrose agar (PDA) medium, potato dextrose (PDB) medium, NaCl, Taq enzyme and PCR related reagents, primers (ITS1 / ITS4), OMEGA...
Embodiment 2
[0042] Bacterial liquid preparation: Inoculate the activated salt-tolerant strain into 60mL potato dextrose medium (PDB) liquid medium, and culture it on a constant temperature shaker for 72h (28°C, 160r·min-1). The concentration of the bacterial solution was calculated by the hemocytometer method, and the obtained concentration was 8.75×10 5 cfu / mL bacterial solution.
[0043] This experiment adopts the soil cultivation pot experiment. The casuarinas selected for the experiment are annual seedlings with an average seedling height of 20cm and an average ground diameter of 3.0mm. They were provided by the Chihu State-owned Forest Farm in Hui'an, Fujian Province. On July 3, 2017, casuarinas with consistent growth were selected and planted in plastic pots with a diameter of 15 cm and a height of 10 cm. The soil required for the experiment was mixed and weighed, and an equal amount (4kg) of yellow core soil was placed in each pot. The nutrient content in the soil is shown in Tab...
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