An endophytic fungus y6 that enhances the antioxidant capacity of casuarina under high-salt environment

A technology of anti-oxidation ability and endophytic fungi, applied in the field of microorganisms, can solve the problems of less research on endophytic fungi in woody plants, and achieve the effect of alleviating damage

Active Publication Date: 2021-07-27
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A series of previous studies have shown that the symbiont between plant endophytic fungi and the host is beneficial for the host to cope with the adverse environment and promote the growth and development of the plant. Less research on endophytic fungi

Method used

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  • An endophytic fungus y6 that enhances the antioxidant capacity of casuarina under high-salt environment
  • An endophytic fungus y6 that enhances the antioxidant capacity of casuarina under high-salt environment
  • An endophytic fungus y6 that enhances the antioxidant capacity of casuarina under high-salt environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation of endophytic fungi of Schima superba

[0021] 1. Main equipment

[0022] AX224ZH electronic balance (OHAUS), LS-75HD high-pressure steam sterilizer (Jinan Laibao Medical Equipment Co., Ltd.), JB-CJ-1500FX ultra-clean workbench (Suzhou Jiabao Purification Engineering Equipment Co., Ltd.), DNP-9162 constant temperature culture box (Shanghai Jinghong), ZHWY2111B constant temperature shaking incubator (Shanghai Zhicheng), Beckman ultracentrifuge (USA), etc. Main instruments for strain identification: FQD-48APCR amplification instrument (BIOER), EPS-100 nucleic acid electrophoresis instrument (Shanghai Tianneng Technology), SeqStudio DNA sequencer (USA), mortar, ice pack, foam box, pipette (Thermo), 1.5ml and 2ml EP tubes (Axygen).

[0023] 2. Main Reagents and Media

[0024] Reagents: 70% alcohol, sodium hypochlorite, potato dextrose agar (PDA) medium, potato dextrose (PDB) medium, NaCl, Taq enzyme and PCR related reagents, primers (ITS1 / ITS4), OMEGA...

Embodiment 2

[0042] Bacterial liquid preparation: Inoculate the activated salt-tolerant strain into 60mL potato dextrose medium (PDB) liquid medium, and culture it on a constant temperature shaker for 72h (28°C, 160r·min-1). The concentration of the bacterial solution was calculated by the hemocytometer method, and the obtained concentration was 8.75×10 5 cfu / mL bacterial solution.

[0043] This experiment adopts the soil cultivation pot experiment. The casuarinas selected for the experiment are annual seedlings with an average seedling height of 20cm and an average ground diameter of 3.0mm. They were provided by the Chihu State-owned Forest Farm in Hui'an, Fujian Province. On July 3, 2017, casuarinas with consistent growth were selected and planted in plastic pots with a diameter of 15 cm and a height of 10 cm. The soil required for the experiment was mixed and weighed, and an equal amount (4kg) of yellow core soil was placed in each pot. The nutrient content in the soil is shown in Tab...

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Abstract

The invention discloses an endophytic fungus Y6 which can increase the antioxidant capacity of casuarina under high-salt environment, and belongs to the technical field of microorganisms. The taxonomic name of this endophytic fungus Y6 is Cyclobacterium ( Hypoxylon sp. ), which was deposited in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on November 20, 2019, with the preservation number CGMCC NO.18814. The endophytic fungus Y6 bacterial solution was applied to the planting of casuarina seedlings in high-salt environment through rhizosphere soil irrigation or direct inoculation of seedlings, which could increase the antioxidant capacity of casuarina.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an endophytic fungus Y6 which can increase the antioxidant capacity of casuarina under a high-salt environment. Background technique [0002] Salt stress seriously affects the growth and development of crops and forest plants, and is the main abiotic stress worldwide. Excess salt ions, such as Na + 、K + , Cl - After being ingested by plants, the ion concentration in plant cells will increase, and various biological enzymes in plant cells can only be active in a narrow range of ion concentrations, such as Na + ions and Cl - Ion requirements below 50mM for K + For ions, it is 0.1-0.2M. Therefore, changes in ion concentration affect the activity of biological enzymes, thereby affecting the growth of plants. Salt stress can also damage the plant cell membrane function by affecting a series of changes such as the composition, permeability, and transport of the plas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00C12R1/645
CPCC12N1/145C12R2001/645
Inventor 李键龙凤林勇明洪滔陈灿
Owner FUJIAN AGRI & FORESTRY UNIV
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