Method for culturing tissues of Buxus sempervives

A technology for tissue culture and golden-leaf boxwood is applied in the field of tissue culture of herbs to achieve the effects of increasing survival rate, increasing yield and increasing reproduction speed

Active Publication Date: 2013-03-06
上海上房园艺有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on tissue culture of Buxus aureus in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for tissue culture of Buxus aureus, the method comprising the following steps:

[0023] (1) Aseptic treatment

[0024] Pick the small buds from the upper part of Euonymus aureus, rinse them with tap water for 2 hours, put them on a clean bench, soak them in 75wt% ethanol for 30s, soak them in 1wt‰ mercury chloride solution for 15mins, and rinse them with sterile water for 5-6 times Afterwards, dry the surface water with sterile filter paper, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA2.0mg / L+NAA0.2mg / L;

[0025] (2) Differentiation and proliferation of buds

[0026] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA2.0mg / L+NAA0.2mg / L was cultu...

Embodiment 2

[0034] A method for tissue culture of Buxus aureus, the method comprising the following steps:

[0035] (1) Aseptic treatment

[0036] Pick the small buds from the upper part of Buxus aureus, rinse them with tap water for 2 hours, put them on a clean bench, soak them in 75wt% ethanol for 20s, soak them in 1wt‰ mercury chloride solution for 10mins, and rinse them with sterile water for 5-6 times Then use sterile filter paper to dry the surface water, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA1.0mg / L+NAA0.1mg / L;

[0037] (2) Differentiation and proliferation of buds

[0038] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA1.0mg / L+NAA0.1mg / L was cultured, and...

Embodiment 3

[0046] A method for tissue culture of Buxus aureus, the method comprising the following steps:

[0047] (1) Aseptic treatment

[0048] Pick the small buds from the upper part of Euonymus aureus, rinse them with tap water for 2 hours, put them on a clean bench, soak them in 75wt% ethanol for 40s, soak them in 1wt‰ mercury chloride solution for 20mins, and rinse them with sterile water for 5-6 times Then use sterile filter paper to blot the surface moisture, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA3.0mg / L+NAA0.3mg / L;

[0049] (2) Differentiation and proliferation of buds

[0050] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA3.0mg / L+NAA0.3mg / L was cultur...

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PUM

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Abstract

The invention relates to a method for culturing tissues of Buxus sempervives. The method comprises the following steps of: picking budlets on the upper part of the Buxus sempervives, and sequentially performing sterile treatment, differentiating and proliferating buds, performing hardening-off culture of adventitious buds, performing rooting culture, exercising seedlings and transplanting to obtain seedlings of the Buxus sempervives. Compared with the prior art, the method has the advantages that: by a tissue culture technology, the reproductive speed and the uniformity of nursery stocks can be greatly improved, the original maternal character can be better maintained, and the survival rate can be improved, so that the yield is improved; and the transplantation survival rate can reach 95 percent.

Description

technical field [0001] The invention relates to a method for tissue culture of herbal plants, in particular to a method for tissue culture of Buxus aureus. Background technique [0002] Euonymus aureus is an evergreen shrub with a compact plant shape and bright yellow new leaves that turn dark green with yellow spots when mature. It likes light and is more cold-resistant. It can survive the winter on land in Beijing. It likes warm and humid climate and fertile and well-drained soil. It is a good ornamental shrub for flower mirrors and gardens, and can also be used as hedgerows or molding materials. Buxus aureus is a species imported from abroad with excellent ornamental value, but the number of introduced species is small, its propagation is slow, the market demand is large, and the supply of seedlings is limited. Through tissue culture technology, the propagation speed and the uniformity of seedlings are greatly improved, and the original female parent traits are better ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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