Tissue culture method of Hemerocallis dumortieri

A technique for cultivating Hemerocallis Hemerocallis and tissue culture, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions to achieve the effects of increasing the reproduction speed and improving the uniformity of seedlings

Inactive Publication Date: 2012-01-04
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a variety imported from abroad, the number of introductions of this flower is small, the ramets are slow, and the supply of seedlings is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Obtaining sterile materials

[0028] Pick the flower buds of "Jinhuang" evergreen daylilies when they bloom, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 60% for 10 seconds, and then soak them in mercury with a volume concentration of 0.5‰ After 10 min, rinse with sterile water for 4 times, use sterile filter paper to blot the water on the surface of the bud, cut the base of the bud into 0.5cm long segments, and inoculate in the culture medium including MS+KT0.1mg / L+NAA1.0mg / On the flower bud induction medium of L;

[0029] (2) Differentiation and proliferation of buds

[0030] The flower buds were inoculated on the flower bud induction medium, they began to expand after 1 week, and yellow-green protrusions appeared, and callus tissue was visible after 3 weeks, and then cultured for 1 month, the callus tissue was divided, cut and inserted into the culture medium including MS+6 -BA0.5...

Embodiment 2

[0039] (1) Obtaining sterile materials

[0040] Pick the flower buds of "Jinhuang" evergreen daylilies when they bloom, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 30 seconds, and then soak them in mercury with a volume concentration of 1‰ After 15 minutes, rinse with sterile water for 5 times, use sterile filter paper to blot the water on the surface of the bud, cut the base of the bud into 1cm-long segments, and inoculate in the culture medium including MS+KT0.5mg / L+NAA3.0mg / L on the flower bud induction medium;

[0041] (2) Differentiation and proliferation of buds

[0042] The flower buds were inoculated on the flower bud induction medium. After 2 weeks, they began to expand and yellow-green protrusions appeared. After 3 weeks, callus tissue was visible. After culturing for 1 month, the callus tissue was divided, cut and inserted into the culture medium including MS+6 -BA2.0mg / L+NA...

Embodiment 3

[0051] (1) Obtaining sterile materials

[0052] Pick the flower buds of "Jinhuang" evergreen daylilies when they bloom, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and then soak them in mercury with a volume concentration of 2‰ After 30 minutes, rinse with sterile water for 6 times, use sterile filter paper to dry the water on the surface of the buds, cut the base of the buds into 2cm-long segments, and inoculate them in the culture medium including MS+KT1. on the flower bud induction medium;

[0053](2) Differentiation and proliferation of buds

[0054] The flower buds were inoculated on the flower bud induction medium. After 3 weeks, they began to expand and yellow-green protrusions appeared. After 5 weeks, callus tissue was visible. After culturing for another 2 months, the callus tissue was divided, cut and inserted into the culture medium including MS+6 -BA4.0mg / L+NAA0...

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PUM

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Abstract

The invention relates to a tissue culture method of Hemerocallis dumortieri, comprising the steps of: acquisition of bacteria-free material, differentiation and proliferation of bud, sound seeding of adventitious bud, rooting culture, hardening and transplantation and the like. Compared with the prior art, the tissue culture method greatly enhances reproduction speed of Hemerocallis dumortieri and uniformity of seedlings, better maintains original maternal character, and can realize batch production of seedlings in factory.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of Jinhuang evergreen Hemerocallis grandiflorum. Background technique [0002] "Jinhuang" evergreen Hemerocallis is a plant of the genus Hemerocallis in the Liliaceae family. It is native to southern Europe through northern Asia to the Yangtze River Basin in Japan. It is a perennial evergreen herb with short rhizomes and thick spindle-shaped fleshy roots. , wide linear, tender green. It blooms in early summer, the flowers are large, funnel-shaped, about 10cm in diameter, and the perianth lobes are oblong and multiple, golden yellow. The flowering period of "Jinhuang" evergreen daylilies is from early June to mid-July. The flowers are bright and easy to cultivate. They are evergreen, and the green leaves are in clusters, which is very beautiful. Many clusters are planted in gardens or in flower borders and roads. beside. "Jinhuang" evergreen Hemerocallis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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