Tissue culture method of Hemerocallis dumortieri
A technique for cultivating Hemerocallis Hemerocallis and tissue culture, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions to achieve the effects of increasing the reproduction speed and improving the uniformity of seedlings
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Embodiment 1
[0027] (1) Obtaining sterile materials
[0028] Pick the flower buds of "Jinhuang" evergreen daylilies when they bloom, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 60% for 10 seconds, and then soak them in mercury with a volume concentration of 0.5‰ After 10 min, rinse with sterile water for 4 times, use sterile filter paper to blot the water on the surface of the bud, cut the base of the bud into 0.5cm long segments, and inoculate in the culture medium including MS+KT0.1mg / L+NAA1.0mg / On the flower bud induction medium of L;
[0029] (2) Differentiation and proliferation of buds
[0030] The flower buds were inoculated on the flower bud induction medium, they began to expand after 1 week, and yellow-green protrusions appeared, and callus tissue was visible after 3 weeks, and then cultured for 1 month, the callus tissue was divided, cut and inserted into the culture medium including MS+6 -BA0.5...
Embodiment 2
[0039] (1) Obtaining sterile materials
[0040] Pick the flower buds of "Jinhuang" evergreen daylilies when they bloom, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 30 seconds, and then soak them in mercury with a volume concentration of 1‰ After 15 minutes, rinse with sterile water for 5 times, use sterile filter paper to blot the water on the surface of the bud, cut the base of the bud into 1cm-long segments, and inoculate in the culture medium including MS+KT0.5mg / L+NAA3.0mg / L on the flower bud induction medium;
[0041] (2) Differentiation and proliferation of buds
[0042] The flower buds were inoculated on the flower bud induction medium. After 2 weeks, they began to expand and yellow-green protrusions appeared. After 3 weeks, callus tissue was visible. After culturing for 1 month, the callus tissue was divided, cut and inserted into the culture medium including MS+6 -BA2.0mg / L+NA...
Embodiment 3
[0051] (1) Obtaining sterile materials
[0052] Pick the flower buds of "Jinhuang" evergreen daylilies when they bloom, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and then soak them in mercury with a volume concentration of 2‰ After 30 minutes, rinse with sterile water for 6 times, use sterile filter paper to dry the water on the surface of the buds, cut the base of the buds into 2cm-long segments, and inoculate them in the culture medium including MS+KT1. on the flower bud induction medium;
[0053](2) Differentiation and proliferation of buds
[0054] The flower buds were inoculated on the flower bud induction medium. After 3 weeks, they began to expand and yellow-green protrusions appeared. After 5 weeks, callus tissue was visible. After culturing for another 2 months, the callus tissue was divided, cut and inserted into the culture medium including MS+6 -BA4.0mg / L+NAA0...
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