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A kind of cultivation method of alum root 'matcha' seedling

A cultivation method and technology of alum root, applied in the field of plant cultivation, can solve the problems of long division propagation period, limited supply of seedlings, poor seedling consistency, etc., to reduce the variation of test-tube seedlings, avoid the variation of test-tube seedlings, and increase the propagation speed Effect

Inactive Publication Date: 2019-11-26
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many varieties of alum root, and some varieties can be sown and propagated, but the seeds germinate slowly, the emergence of seedlings is irregular or the consistency of seedlings is poor; the propagation cycle of ramets is long and the reproduction coefficient is low. Although the market demand is large, the supply of seedlings is limited, which hinders new varieties Promoted application of
'Matcha' is an alum root variety with green leaves and red flowers. It has grown well in southern regions such as Shanghai after planting observations, but the seedling breeding technology of this variety has not been reported so far.

Method used

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  • A kind of cultivation method of alum root 'matcha' seedling
  • A kind of cultivation method of alum root 'matcha' seedling
  • A kind of cultivation method of alum root 'matcha' seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1~9

[0037] Using the stem base tissue of alum root 'Matcha' in spring and summer as explants, the explants are first sterilized. The aseptic treatment is to rinse the explants with running water for 1.5 hours, and then place them on an ultra-clean workbench. Above; soak in ethanol with a mass fraction of 75% for 30-40s; then soak in sodium hypochlorite solution (adding 1-2 drops of Tween) with an available chlorine concentration of 0.4%-0.6% for 10-12min; finally rinse with sterile water for 4 -After 5 times, blot the surface moisture with sterile gauze;

[0038] Next, cut the stem base of the alum root 'Matcha' after the aseptic treatment into 0.8-1.2 cm lengths and inoculate them on the induction medium for clustering buds, inoculate 3 explants in each bottle, and inoculate 6 bottles for each treatment; , the induction medium of clustered buds corresponds to MS basic medium, MS+6-BA0.5mg / L+NAA0.1mg / L, MS+6-BA1.0mg / L+NAA0.1mg / L respectively according to embodiment 1~9 L, MS+6-BA...

Embodiment 10~25

[0049] After clustering buds are divided into single buds, they are respectively inoculated in the proliferation medium for proliferation culture. Each bottle is inoculated with 4 small buds as a treatment, and each treatment is inoculated with 6 bottles. The proliferation culture period is 30 days; Examples 10-25 correspond to MS basic medium, MS+KT0.1mg / L+IAA0.2mg / L, MS+KT0.5mg / L+IAA0.2mg / L, MS+KT1.0mg / L+IAA0.2mg / L, MS+KT2.0mg / L+IAA0.2mg / L, MS+KT2.5mg / L+IAA0.2mg / L, MS+6-BA0.1mg / L+AA0.2mg / L, MS+6 -BA0.5mg / L+IAA0.2mg / L, MS+6-BA1.0mg / L+IAA0.2mg / L, MS+6-BA2.0mg / L+IAA0.2mg / L, MS+6-BA0 .2mg / L+KT0.5mg / L+IAA0.2mg / L, MS+6-BA0.2mg / L+KT1.0mg / L+IAA0.2mg / L, MS+6-BA0.3mg / L+KT0 .5mg / L+IAA0.2mg / L, MS+6-BA0.3mg / L+KT1mg / L+IAA0.2mg / L, MS+6-BA0.5mg / L+KT0.2mg / L+IAA0.2mg / L.

[0050] The test results are shown in Table 2, which shows that:

[0051] (1) In combination with Example 10 in Table 2, the single bud of 'Matcha' hardly proliferates on the MS medium without plant growth regulators. A...

Embodiment 26~38

[0060] After a certain amount of clustered buds were obtained from the multiplication culture, the clustered shoots were divided into individual sprouts, the old leaves at the base were gently pulled off, and they were respectively inoculated in the rooting medium to induce rooting. Each bottle was inoculated with 8 plants, and each treatment was inoculated with 4 bottles. 25 Complete plants were formed after -30d; wherein, the proliferation medium corresponds to 1 / 2MS basic medium, 1 / 2MS+IBA0.2mg / L, 1 / 2MS+IBA0.5mg / L, 1 / 2MS respectively according to Examples 26-38 +IBA1.0mg / L, 1 / 2MS+IBA1.5mg / L, 1 / 2MS+NAA0.1mg / L, 1 / 2MS+NAA0.2mg / L, 1 / 2MS+NAA0.5mg / L, 1 / 2MS +NAA1.0mg / L, 1 / 2MS+IBA0.5mg / L+NAA0.1mg / L, 1 / 2MS+IBA0.5mg / L+NAA0.2mg / L, 1 / 2MS+IBA0.5mg / L+NAA0 .3mg / L, 1 / 2MS+IBA0.5mg / L+NAA0.4mg / L.

[0061] The results are shown in Table 3, showing that:

[0062] (1) In conjunction with Example 26 in Table 3, on the MS medium without plant growth regulators, the alum root 'Matcha' tissue cult...

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Abstract

The invention discloses a sweetleaf root 'matcha' seedling culture method which includes the steps: digging sweetleaf root 'matcha' stem base tissues in spring and summer, performing bacteria-free treatment, and inoculating the tissues on a multiple-shoot inducement culture medium for multiple-shoot inducement culture to obtain multiple shoots 30 days later; dividing the multiple shoots and placing the multiple shoots into a proliferation culture medium for proliferation culture with the cycle of 30 days; acquiring a certain number of multiple shoots by proliferation culture, dividing the multiple shoots into single bud seedlings, culturing the bud seedlings in a rooting culture medium for rooting induction, and forming a complete plant 25-30 days later; hardening the seedlings with a bottle cap in an environment with scattered light for 3-7 days, and then hardening the seedlings in an uncapped manner for 1-2 days; taking out test-tube seedlings after hardening, washing a root culturemedium and transplanting the seedlings into a seedling culture plug. Compared with the prior art, the sweetleaf root 'matcha' seedling culture method has the advantages that sweetleaf root 'matcha' seedlings are reproduced by a tissue culture technology, operation is simple, reproductive rate is high, the problem of too low speed of division propagation can be solved, and mass production of the sweetleaf root 'matcha' seedlings with consistent characters is realized.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and relates to a tissue culture method of a perennial color-leaved herb, in particular to a method for cultivating alum root 'Matcha' seedlings. Background technique [0002] Alumroot is a perennial herbaceous flower with colorful leaves native to Central America. In recent years, alum root has been introduced to China, and has gradually attracted attention due to its beautiful flowers and leaves, bright and rich colors. Alum root can be used as materials such as edging, clump planting, ground cover, potted plant, etc., and it continues to heat up in the flower market. Alum root is also introduced and planted in a small amount in southern regions such as Shanghai. The colorful leaf plant does not lose its leaves in winter in warm southern regions such as Shanghai. It can be used as a ground cover to supplement the lack of winter landscape color; it can also be used as a potted plant to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 殷丽青蔡友铭吴杰孙翊张永春庹晓庆杨柳燕
Owner SHANGHAI ACAD OF AGRI SCI
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