Tissue culture method for heuchera micrantha 'Palace Purple'

A tissue culture, coral clock technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of small number of introductions, slow ramet propagation, etc. The effect of uniformity

Inactive Publication Date: 2012-01-04
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a new variety introduced from abroad, the number of introduced species of this plant is small, and the division propagation is slow. The demand on the market is limited by the supply of seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Obtaining sterile materials

[0028] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and then soak them in mercury with a volume concentration of 0.5‰ for 10 minutes. Rinse 4 times with sterile water, use sterile filter paper to blot the water on the surface of the buds, cut the buds into 0.5cm-long segments with axillary buds, and inoculate them with MS+6-BA1.0mg / L+NAA0.1mg / L bud induction medium;

[0029] (2) Differentiation and proliferation of buds

[0030] One week after the segments were inoculated on the axillary bud induction medium, the axillary buds began to expand and green protrusions appeared, and after 3 weeks, bud meristems could be seen. After culturing for another month, the small buds formed clusters, and the adventitious buds were cut off and transferred to the MS+6-BA0.5mg / L+NAA0.05mg / L ...

Embodiment 2

[0039] (1) Obtaining sterile materials

[0040] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 30 seconds, and then soak them in mercury with a volume concentration of 1‰ for 15 minutes. Rinse 5 times with sterile water, use sterile filter paper to blot the water on the surface of the buds, cut the buds into 1cm-long segments with axillary buds, and inoculate them in a medium containing MS+6-BA2.0mg / L+NAA0.2mg / On the shoot induction medium of L;

[0041] (2) Differentiation and proliferation of buds

[0042] After the segments were inoculated on the axillary bud induction medium for 2 weeks, the axillary buds began to expand and green protrusions appeared. After 3 weeks, bud meristems could be seen. After culturing for another month, the small buds formed clusters. Cut off the adventitious buds and transfer them to MS+6-BA2.0mg...

Embodiment 3

[0051] (1) Obtaining sterile materials

[0052] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and then soak them in mercury with a volume concentration of 2‰ for 30 minutes. Rinse 6 times with sterile water, use sterile filter paper to blot the water on the surface of the buds, cut the buds into 2cm-long segments with axillary buds, and inoculate them in a medium containing MS+6-BA3.0mg / L+NAA0.3mg / On the shoot induction medium of L;

[0053] (2) Differentiation and proliferation of buds

[0054]After the segments were inoculated on the axillary bud induction medium for 3 weeks, the axillary buds began to expand and green protrusions appeared, and after 5 weeks, bud meristems could be seen. After 2 months of culture, the small buds formed clusters, and the adventitious buds were cut off and transferred to the MS+6-B...

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PUM

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Abstract

The invention relates to a tissue culture method for heuchera micrantha 'Palace Purple'. The method comprises the following steps of: obtaining sterile materials; differentiating and proliferating buds; culturing strong seedlings of adventitious buds; rooting; and hardening off seedlings and transplanting the seedlings and the like. Compared with the prior art, the method greatly improves the reproductive speed of the heuchera micrantha 'Palace Purple' and the trimming of the seedlings, and improves the stability of shape and properties of the heuchera micrantha 'Palace Purple', so that the industrial mass production of the seedlings can be realized.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to the tissue culture method of the purple leaf coral bell. Background technique [0002] Purple leaf coral bell is a plant of the saxifrage family Alumroot. Native to North America, it is a perennial flower with purplish red leaves, prefers half shade, and can withstand full light; it is mostly used in forest flower borders, ground covers, garden greening, etc. in gardens, and the flowering period is from April to October. It is an ideal color Leaf perennial flower border material. However, as a new variety introduced from abroad, the number of introduced species of this plant is small, and the division propagation is slow. The demand on the market is limited by the supply of seedlings. Contents of the invention [0003] The purpose of the present invention is exactly to provide a kind of tissue culture method of the purple leaf coral bell that can improve the propagation speed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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