Tissue culture method of nandina flame
A technology of nandina and flame, which is applied in the field of tissue culture of nandina 'flame', can solve the problems of cutting, slow branching, low cutting survival rate and small number, etc., and achieve the effect of increasing the speed of reproduction and
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Embodiment 1
[0028] The tissue culture method of nandina 'flame', the method comprises the following steps:
[0029] (1) Obtaining sterile materials
[0030] Pick the newly germinated buds of Nantian bamboo, rinse them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 20s and 1wt‰mercuric chloride solution for 10mins, rinse them with sterile water for 5-6 times and remove them. Blot the surface moisture, take the base of the buds and cut them into 1cm and inoculate them in the bud induction medium. The composition of the bud induction medium is MS+6-BA1.0mg / L+NAA0.1mg / L+IBA1.0mg / L L;
[0031] (2) Differentiation and proliferation of buds
[0032] Four weeks after inoculation of the small buds on the small bud induction medium, the small buds began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks. Proliferation, adventitious buds grow rapidly without vitrification and other adverse phenom...
Embodiment 2
[0041] The tissue culture method of nandina 'flame', the method comprises the following steps:
[0042] (1) Obtaining sterile materials
[0043] Pick the newly germinated buds of Nantian bamboo, rinse them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 40s and 1wt‰mercuric chloride solution for 20mins, rinse them with sterile water for 6 times and blot them dry Surface water, take the base of the budlet and cut it into 1cm and inoculate it in the budlet induction medium, the composition of the budlet induction medium is MS+6-BA3.0mg / L+NAA0.3mg / L+IBA3.0mg / L;
[0044] (2) Differentiation and proliferation of buds
[0045] Four weeks after the budlets were inoculated on the budlet induction medium, the budlets began to swell, and yellow-green protrusions appeared, and obvious callus tissue could be seen after 2 weeks. Proliferation, adventitious buds grow rapidly without vitrification and other adverse phenomena, they grow well...
Embodiment 3
[0054] The tissue culture method of nandina 'flame', the method comprises the following steps:
[0055] (1) Obtaining sterile materials
[0056] Pick the newly germinated buds of Nantian bamboo, rinse them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 30s and 1wt‰mercuric chloride solution for 15mins, rinse them with sterile water for 5 times and blot them dry Surface moisture, take the base of the budlet and cut it into 1cm and inoculate it in the budlet induction medium, the composition of the budlet induction medium is MS+6-BA5.0mg / L+NAA0.5mg / L+IBA5.0mg / L;
[0057] (2) Differentiation and proliferation of buds
[0058] Four weeks after inoculation of the small buds on the small bud induction medium, the small buds began to swell, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks, and there were more small buds on the basal callus, but it did not affect the adventitious buds Advent...
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