Tissue culture method of baptisia australis

A technology of tissue culture and culture medium, applied in the field of plant tissue culture, which can solve the problems of large market demand, slow branching, limited supply of seedlings, etc., and achieve the effect of increasing the reproduction speed and maintaining the traits of the female parent

Inactive Publication Date: 2014-04-30
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

New varieties introduced from abroad, the number of introductions is small, the ramets are slow, the market demand is large, and the supply of seedlings is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for tissue culturing Australian lentils, using the following steps:

[0030] (1) Aseptic treatment

[0031] Pick the newly germinated terminal buds of Australian blue bean plants, wash them with water for 2 hours, soak them in 75v / v% ethanol for 30s, soak them in 1v / v%0 liter mercury for 15mins, and then wash them with sterile water for 5-6 times. Blot the surface moisture, cut the buds into 1em and inoculate them on the bud induction medium. The nutritional composition of the bud induction medium is MS+6-BA1.0mg / L+NAA0.1mg / L, and also includes sucrose 30g / L, The basic composition of agar 6g / L, the pH value of the medium is 5.8, the control culture temperature is 24 ℃, and the light is 70 μmol / ms, and the bud induction culture is carried out;

[0032] (2) differentiation and proliferation

[0033] Calli can be seen in the shoots inoculated on the bud induction medium after 4 weeks of culture, and after 1 month of culture, the callus with buds is inoculated on...

Embodiment 2

[0041] A method for tissue culturing Australian lentils, using the following steps:

[0042] (1) Aseptic treatment

[0043] Pick the newly germinated terminal buds of Australian blue bean plants, wash them with water for 2 hours, soak them in 75v / v% ethanol for 30s, soak them in 1v / v%0 liter mercury for 15mins, rinse them with sterile water for 5 times, and blot them dry with sterile filter paper Surface moisture, bud segment is cut into 1cm and inoculated on the bud induction medium, the nutrient composition of the bud induction medium is MS+6-BA3.0mg / L+NAA0.3mg / L, also includes sucrose 30g / L, agar 6g / L of the basic components, the pH value of the medium is 5.8, the control culture temperature is 25°C, and the light is 80 μmol / ms, and the bud induction culture is carried out;

[0044] (2) differentiation and proliferation

[0045] The bud segment inoculated on the bud induction medium can be seen as callus tissue after 4 weeks of culture, and after culturing for 1 month, t...

Embodiment 3

[0053] A method for tissue culturing Australian lentils, using the following steps:

[0054] (1) Aseptic treatment

[0055] Pick the newly germinated terminal buds of Australian blue bean plants, wash them with water for 2 hours, soak them in 75v / v% ethanol for 30s, soak them in 1v / v%0L mercury for 15mins, rinse them with sterile water for 6 times, and blot them dry with sterile filter paper Surface moisture, cut the buds into 1cm and inoculate them on the bud induction medium. The nutritional composition of the bud induction medium is MS+6-BA5.0mg / L+NAA0.5mg / L, including 30g / L sucrose and 6g agar / L of the basic components, the pH value of the medium is 5.8, the control culture temperature is 26°C, and the light is 90 μmol / ms, and the bud induction culture is carried out;

[0056] (2) differentiation and proliferation

[0057] Calli can be seen in the shoots inoculated on the bud induction medium after 4 weeks of culture, and after 1 month of culture, the callus with buds i...

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PUM

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Abstract

The invention relates to a tissue culture method of baptisia australis. The tissue culture method comprises the steps of sterile treatment, differentiation and proliferation, strong seedling of adventitious buds, rooting culture, acclimatization and transplanting, and the like. The step-by-step tissue culture of the baptisia australis is carried out by using a bud induction medium, a proliferation medium, a strong seedling medium and a rooting medium. Compared with the prior art, the tissue culture method has the advantages that the baptisia australis is subjected to step-by-step tissue culture by selecting the appropriate mediums, so that the reproduction speed and the evenness degree of nursery stocks are increased, the original female parent characters are maintained well, and in addition, the survival rate of transplanting can reach 85%.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for tissue culture of australis bean. Background technique [0002] Australian blue bean is a perennial root flower plant of the genus Pseudoindigo in the family Fabaceae. Native to Australia, often grows on forest margins. The stems are erect, about 50-100cm high, and form clumps. Pinnate compound leaves, alternate, raceme born on the top of the stem, [0003] Corolla butterflies, blue. The flowering period is April-May. Fruit oval or oblong; seeds brownish yellow, reniform. Perennial roots can be overwintered in the open air. Strong new shoots sprout from the base in spring. Like cool, well-drained, ventilated, sunny place, avoid hot and humid environment. Australian blue beans are more afraid of dark and humid environments, so the cultivation environment must be ventilated and sunny. It can be planted in large areas as ground cover, or it can be planted in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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