Tissue culture method of dianella ensifolia

A technology of tissue culture of Suga orchid japonica, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as large market demand and limited supply of seedlings, so as to improve the propagation speed and seedling quality The effect of uniformity

Active Publication Date: 2014-04-30
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The market demand is high, and the supply of seedlings is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Procurement of sterile materials

[0024] Take the small buds that germinate from the base of Sugamophyllum japonica, wash them with tap water for 2 hours, put them on a clean bench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, wash them with sterile water 5-6 times, and put them on a clean bench. Blot the water on the surface with filter paper, cut the base of the buds into 1cm lengths, and inoculate them on the bud induction medium. The nutritional components of the medium are MS+6-BA1. The composition is 30g / L sucrose, 6g / L agar, the pH value of the medium is controlled at 5.8, the culture temperature is controlled at 24°C, and the light is 70μmol / ms, and the bud induction culture is carried out.

[0025] Bud differentiation and proliferation

[0026] Four weeks after the budlets were inoculated on the budlet induction medium, the budlets began to swell, and yellow-green protrusions appeared, and obvious callus tissue could ...

Embodiment 2

[0034] Procurement of sterile materials

[0035] Take the small buds that germinate from the base of Sugamophyllum japonica, wash them with tap water for 2 hours, put them on a clean bench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, wash them with sterile water 5-6 times, and put them on a clean bench. Blot the water on the surface with filter paper, cut the base of the buds into 1cm lengths, and inoculate them on the bud induction medium. The nutrient composition of the medium is MS+6-BA2. The composition is 30g / L sucrose, 6g / L agar, the pH value of the medium is controlled at 5.8, the culture temperature is controlled at 24°C, and the light is 70μmol / ms, and the bud induction culture is carried out.

[0036] Bud differentiation and proliferation

[0037] Four weeks after the budlets were inoculated on the budlet induction medium, the budlets began to swell, and yellow-green protrusions appeared, and obvious callus tissue could be ...

Embodiment 3

[0045] Procurement of sterile materials

[0046] Take the small buds that germinate from the base of Sugamophyllum japonica, wash them with tap water for 2 hours, put them on a clean bench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, wash them with sterile water 5-6 times, and put them on a clean bench. Blot the water on the surface with filter paper, cut the base of the buds into 1cm lengths, and inoculate them on the bud induction medium. The nutrient composition of the medium is MS+6-BA3. The composition is 30g / L sucrose, 6g / L agar, the pH value of the medium is controlled at 5.8, the culture temperature is controlled at 25°C, and the light is 80μmol / ms, and the bud induction culture is carried out.

[0047] Bud differentiation and proliferation

[0048] Four weeks after the budlets were inoculated on the budlet induction medium, the budlets began to swell, and yellow-green protrusions appeared, and obvious callus tissue could be ...

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PUM

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Abstract

The invention relates to a tissue culture method of dianella ensifolia. The tissue culture method comprises the steps: respectively using a budlet induction medium for carrying out bud induction culture, a proliferation medium for carrying out proliferation culture, a strong seedling medium for carrying out strong seedling culture and a rooting medium for rooting culture, then carrying out domestication and transplanting so as to obtain dianella ensifolia plants. Compared with the prior art, the tissue culture method has the advantages that the reproduction speed and the evenness degree of seedlings are greatly increased through a tissue culture technology, the original female parent characters are maintained well, and the survival rate of transplanting can reach 95%.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for tissue culture of Suga japonica. Background technique [0002] Huayeshan Suga is a horticultural cultivar of Liliaceae Suga, belonging to perennial herbaceous plants, with a plant height of 50-70 cm; leaves near the base, 2 rows, narrow strip-lanceolate, leathery, 30-60 cm long; The stems are transverse, nodular, with thin and hard roots on the nodes; scapes are pulled out from the leaves, and the panicles are 10-30 cm long, with many flowers, open in summer, lavender, and berries purple-blue. Suga orchid has beautiful plant shape, beautiful leaf color, and silver-white stripes on the edge of the leaf. It is elegant and beautiful, and is deeply loved by gardeners. It is often used as a ground cover plant in gardens. It is often used in forests, garden roadsides, and rocks. , It can also be used as a potted plant indoors. In the garden, it is often planted in clu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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