Method for tissue culture of long-ear kniphofia
A technology of tissue culture and torch flower, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low seed setting rate and slow branching, and achieve the effect of maintaining the traits of the female parent and improving the seed setting rate
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Embodiment 1
[0027] A method for tissue culturing Torchflower long spikes, adopts the following steps:
[0028] (1) Bud induction culture: pick the small buds of Torchia longis, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, and rinse them with sterile water for 5-6 minutes. The second time, dry the surface moisture with sterile filter paper, cut the base of the shoots into 1cm lengths, and inoculate them on the bud induction medium. 6-BA1.0mg / L+NAA0.1mg / L, the pH value is 5.5, the culture temperature is controlled at 24°C, and the light is 100μmol / ms, and the bud induction culture is carried out;
[0029] (2) Bud differentiation and proliferation: 5 weeks after the buds were inoculated on the bud induction medium, the base of the buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. After culturing for 1 month, the ca...
Embodiment 2
[0034] A method for tissue culturing Torchflower long spikes, adopts the following steps:
[0035] (1) Bud induction culture: pick the small buds of Torchia longis, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, and rinse them with sterile water for 5-6 minutes. The second time, dry the surface moisture with sterile filter paper, cut the base of the tender shoots into 1cm lengths, and inoculate them on the bud induction medium. 6-BA2.0mg / L+NAA0.2mg / L, the pH value is 5.7, the control culture temperature is 25°C, the light is 90μmol / ms, and the bud induction culture is carried out:
[0036] (2) Bud differentiation and proliferation: 5 weeks after the buds were inoculated on the bud induction medium, the base of the buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. After culturing for 1 month, the callus...
Embodiment 3
[0041] A method for tissue culturing Torchflower long spikes, adopts the following steps:
[0042](1) Bud induction culture: pick the small buds of Torchia longis, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, and rinse them with sterile water for 5-6 minutes. The second time, dry the surface moisture with sterile filter paper, cut the base of the tender shoots into 1cm lengths, and inoculate them on the bud induction medium. 6-BA3.0mg / L+NAA0.3mg / L, the pH value is 5.8, the culture temperature is controlled at 25°C, the light is 80μmol / ms, and bud induction culture is carried out;
[0043] (2) Bud differentiation and proliferation: 5 weeks after the buds were inoculated on the bud induction medium, the base of the buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. After culturing for 1 month, the callu...
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