Method for tissue culture of long-ear kniphofia

A technology of tissue culture and torch flower, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low seed setting rate and slow branching, and achieve the effect of maintaining the traits of the female parent and improving the seed setting rate

Active Publication Date: 2014-05-07
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long spike torch flower is longer than general torch flower inflorescence, but the seed setting rate is very low, and the branching is slow. Therefore, it is necessary to develop tissue culture technology, improve the seedling propagation speed and seedling uniformity and maintain the original female parent traits, and realize the seedling plant. At present, there is no report on the tissue culture of Torchflower elongatum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for tissue culturing Torchflower long spikes, adopts the following steps:

[0028] (1) Bud induction culture: pick the small buds of Torchia longis, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, and rinse them with sterile water for 5-6 minutes. The second time, dry the surface moisture with sterile filter paper, cut the base of the shoots into 1cm lengths, and inoculate them on the bud induction medium. 6-BA1.0mg / L+NAA0.1mg / L, the pH value is 5.5, the culture temperature is controlled at 24°C, and the light is 100μmol / ms, and the bud induction culture is carried out;

[0029] (2) Bud differentiation and proliferation: 5 weeks after the buds were inoculated on the bud induction medium, the base of the buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. After culturing for 1 month, the ca...

Embodiment 2

[0034] A method for tissue culturing Torchflower long spikes, adopts the following steps:

[0035] (1) Bud induction culture: pick the small buds of Torchia longis, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, and rinse them with sterile water for 5-6 minutes. The second time, dry the surface moisture with sterile filter paper, cut the base of the tender shoots into 1cm lengths, and inoculate them on the bud induction medium. 6-BA2.0mg / L+NAA0.2mg / L, the pH value is 5.7, the control culture temperature is 25°C, the light is 90μmol / ms, and the bud induction culture is carried out:

[0036] (2) Bud differentiation and proliferation: 5 weeks after the buds were inoculated on the bud induction medium, the base of the buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. After culturing for 1 month, the callus...

Embodiment 3

[0041] A method for tissue culturing Torchflower long spikes, adopts the following steps:

[0042](1) Bud induction culture: pick the small buds of Torchia longis, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, and rinse them with sterile water for 5-6 minutes. The second time, dry the surface moisture with sterile filter paper, cut the base of the tender shoots into 1cm lengths, and inoculate them on the bud induction medium. 6-BA3.0mg / L+NAA0.3mg / L, the pH value is 5.8, the culture temperature is controlled at 25°C, the light is 80μmol / ms, and bud induction culture is carried out;

[0043] (2) Bud differentiation and proliferation: 5 weeks after the buds were inoculated on the bud induction medium, the base of the buds began to expand, and yellow-green protrusions appeared. After 3 weeks, obvious callus tissue was visible. After culturing for 1 month, the callu...

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PUM

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Abstract

The invention discloses a method for the tissue culture of long-ear kniphofia. The method comprises the steps of picking up long-ear kniphofia budlet, washing, culturing by a bud induction medium, putting cultured calluses with buds into a multiplication medium for differentiation multiplication, carrying out adventitious bud cultivation and rooting cultivation, and obtaining the long-ear kniphofia plant through the seedling hardening technology and the transplanting technology. Compared with the prior art, according to the tissue culture technology, the maturing rate can be improved, and the different nutrition facts can be provided at the different cultivation stages by the selected bud induction culture medium, multiplication culture medium, seedling boosting culture medium and rooting culture medium, so that the original female characters can be kept to the maximum, and the transplanting survival rate in the steps of seedling hardening and transplanting can reach 90%.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a method for tissue culturing lobula long panicle. Background technique [0002] Torchflower long spike is a perennial herb of the genus Torchflower in the family Liliaceae. Plant height 80-120 cm, stems erect. The raceme bears hundreds of small tubular flowers, which are torch-shaped, and the corolla is orange-red. The flowering period is from June to July. It likes warmth and should grow in loose and fertile sandy loam. The tall flower stems hold up the torch-like inflorescences, which are magnificent. It is suitable for arranging perennial mixed flower borders and configuring in front of buildings. It can be planted in lawns or next to artificial rocks as a background. It can also be used as a background For cut flowers. Long spike torch flower is longer than general torch flower inflorescence, but the seed setting rate is very low, and the branching is slow. Therefore, i...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄建荣申瑞雪陈建华沈勤
Owner 上海上房园艺有限公司
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