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Method for obtaining poinsettia filial generation through utilizing young embryo rescue

A technology of immature embryo rescue and first-class products, applied in the field of biotechnology breeding, to overcome the difficulty of obtaining hybrids, shorten the growth cycle, and improve the success rate

Inactive Publication Date: 2013-01-02
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since Ehanning first succeeded in in vitro culture of embryos using Brassicaceae plants as test materials in 1904, immature embryo rescue technology, as one of the most effective means to overcome embryo abortion after fertilization, has been used in field crops such as wheat and rapeseed. And fruit trees, vegetables, flowers and other horticultural crop hybrid breeding fields are widely used, but there is no relevant report in poinsettia hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The method for rescuing immature ovule embryos after embodiment one poinsettia artificial hybridization pollination 15d

[0023] 1. Artificial cross-pollination: Select and determine the hybrid parents of poinsettia, and control the flowering period and keep the flowering period consistent through shading or supplementary light adjustment. Pollination is carried out between 11:00 and 12:00 in the morning on a sunny day. Fresh pollen collected from the male parent's stigma on the same day is gently transferred to the inverted "eight"-shaped stigma of the female parent with a brush; pollination is repeated once the next day. The ambient temperature of the greenhouse should not be lower than 25°C and should be managed normally.

[0024] 2. Stripping of hybrid ovules and induction culture: take poinsettia ovaries that have developed 15 days after cross-pollination, wash them under running water for 5 minutes, soak them in ethanol with a volume ratio of 75% for 1 minute unde...

Embodiment 2

[0027] The ovule immature embryo rescue method after embodiment two poinsettia artificial hybridization pollination 22d

[0028] 1 item is the same as embodiment one.

[0029] 2. Extraction of hybrid ovules and induction culture: take poinsettia hybrid ovary developed 22 days after pollination, wash it under running water for 5 minutes, soak it in ethanol with a volume ratio of 75% for 1 minute under sterile conditions, and then use a mass percentage of 0.1% Disinfect in mercuric solution for 10 minutes, rinse with sterile water for 4 to 5 times, tear off the ovary wall with tweezers, gently peel off the ovules, and quickly inoculate them with MS+NAA 0.5mg / L+6-BA 0.5mg / L for culture Cultivate on medium, medium pH 5.6-5.8, sucrose 60g / L, agar 6g / L, at a temperature of 20-25°C, a photoperiod of 10-12h / d, and a light intensity of 1500-2000lux. Culture directly induces germination of immature embryos.

[0030]3. Subculture, proliferation and rooting culture: After 30 days, trans...

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Abstract

The invention discloses a method for obtaining a poinsettia filial generation through utilizing young embryo rescue. The method is characterized in that 15-22d of ovules which are subjected to artificial cross pollination are subjected to young embryo rescue through adopting in-vitro culture; and the method comprises the following specific steps of: (1) ovule stripping, (2) induction culturing, (3) subculturing, multiplying and rooting, and (4) transplanting. By utilizing the method, poinsettia hybrid ovules are effectively prevented from premature failure, the hybrid survival rate of the poinsettia filial generation is improved, the culture period of the poinsettia filial generation is shortened, and the method can be applied to poinsettia hybridization breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to a method for obtaining poinsettia hybrid offspring by rescuing immature embryos. Background technique [0002] Poinsettia (Euphorbia pulcherrima willd), native to tropical Mexico and Central America, is an evergreen and semi-evergreen perennial upright shrub of the Euphorbia family (Euphorbiaceae). Its natural "flowering" period is from November to March of the following year, which coincides with In winter festivals, the leaf-shaped bracts on the top of the main ornamental part are huge and colorful. It is one of the traditional potted flowers in European and American countries. The large-scale commercial production of poinsettia in my country began in 1998. Due to the introduction of new and superior varieties and advanced cultivation techniques, the market share has increased year by year. [0003] At present, hundreds of varieties with different plant types,...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 李畅苏家乐刘晓青陈尚平何丽斯
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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