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Method of utilizing sodium azide to induce bud mutation of poinsettia

A technology of sodium azide and first grade is applied in the field of using sodium azide to induce poinsettia bud transformation, which can solve the problems of small natural mutation rate of poinsettia and inability to actively carry out large-scale resource innovation of poinsettia, and achieve the effect of shortening the breeding period.

Inactive Publication Date: 2018-04-17
东莞市粮作花卉研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] This patent technology proposes a treatment method for sodium azide-induced poinsettia bud change. This method clarifies the conditions such as the concentration and treatment method for sodium azide to effectively induce poinsettia axillary bud variation, and is easy to develop and effectively applied to poinsettia bud change breeding. , to solve the problems that the natural variation rate of poinsettia is small, and it is impossible to actively carry out large-scale innovation of poinsettia resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Solution preparation:

[0016] ① Buffer preparation

[0017] Weigh the medicine Potassium Dihydrogen Phosphate (KH 2 PO 4 ) 0.27g, disodium hydrogen phosphate (Na 2 HPO 4 ) 1.42g, sodium chloride (NaCl) 8g, potassium chloride (KCl) 0.2g, add about 800mL of deionized water and stir to dissolve, then add concentrated hydrochloric acid to adjust the pH to 7.4, and finally dilute to 1L, and prepare PBS buffer liquid.

[0018] ②Preparation of sodium azide solution

[0019] Weigh the medicine in the fume hood, weigh 0.1gNaN 3 , add phosphate buffer to the beaker to dissolve, use a glass rod to dissolve the NaN 3 Transfer the solution to a 100ml volumetric flask and rinse the beaker with buffer several times to ensure NaN 3 Transfer completely, dilute to 100ml with phosphate buffer, and prepare 0.1% NaN 3 solution. Gradiently dilute the 0.1% sodium azide mother solution to a concentration of 0.06% for later use.

Embodiment 2

[0021] (1) Solution preparation:

[0022] ① Buffer preparation

[0023] Weigh the medicine Potassium Dihydrogen Phosphate (KH 2 PO 4 )0.1g, disodium hydrogen phosphate (Na 2 HPO 4 ) 1g, sodium chloride (NaCl) 5g, potassium chloride (KCl) 0.5g, add about 800mL of deionized water and stir to dissolve, then add concentrated hydrochloric acid to adjust the pH to 7.4, and finally dilute to 1L to prepare PBS buffer .

[0024] ②Preparation of sodium azide solution

[0025] Weigh the medicine in the fume hood, weigh 0.03gNaN 3 , add phosphate buffer to the beaker to dissolve, use a glass rod to dissolve the NaN 3 Transfer the solution to a 100ml volumetric flask and rinse the beaker with buffer several times to ensure NaN 3 Transfer completely, dilute to 100ml with phosphate buffer, and prepare 0.03% NaN3 solution.

Embodiment 3

[0027] (1) Solution preparation:

[0028] ① Buffer preparation

[0029] Weigh the medicine Potassium Dihydrogen Phosphate (KH 2 PO 4 )0.2g, disodium hydrogen phosphate (Na 2 HPO 4 ) 1.6g, sodium chloride (NaCl) 10g, potassium chloride (KCl) 0.1g, add about 800mL of deionized water and stir to dissolve, then add concentrated hydrochloric acid to adjust the pH to 7.4, and finally adjust the volume to 1L, and prepare PBS buffer liquid.

[0030] ②Preparation of sodium azide solution

[0031] Weigh the medicine in the fume hood, weigh 0.08gNaN 3 , add phosphate buffer to the beaker to dissolve, use a glass rod to dissolve the NaN 3 Transfer the solution to a 100ml volumetric flask and rinse the beaker with buffer several times to ensure NaN 3 Transfer completely, dilute to 100ml with phosphate buffer, and prepare 0.08% NaN 3 solution.

[0032] Poinsettia trial treatment:

[0033] ①Before the arrival of short-day sunshine in September-October every year, the poinsettia ad...

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Abstract

The invention discloses a method of utilizing sodium azide to induce bud mutation of poinsettia. The method includes treatment steps: using a scalpel to pinch poinsettia, reserving an internode on anaxillary bud of 2-5cm during pinching, using a liquid transfer gun to extract 100-500ml of NaN3 solution, injecting into the internode, and wrapping a notch; putting treated poinsettia in a greenhousefor culture, watering, fertilizing, and waiting for a new bud to grow out after 1 month, wherein concentration of the NaN3 solution is 0.02-0.08%. The method specifies conditions like concentration and treatment methods for effectively inducing bud mutation of poinsettia, is simple and convenient in research and development and can be effectively applied in bud mutation breeding of poinsettia, and the problem that poinsettia is small in natural mutation rate and the problem that resource innovation of poinsettia cannot be performed actively on a large-scale are solved.

Description

technical field [0001] The invention relates to the technical field of plant mutation, in particular to a method for inducing poinsettia bud change by using sodium azide. Background technique [0002] Sodium azide (NaN3) has the advantages of no residual toxicity and relatively safe use, but there is no research report on the method of using sodium azide to induce poinsettia bud change in China so far. Sodium azide has been used in the breeding research of plants such as barley, rice, sunflower, soybean, flower and apple, mostly for mutagenesis of plant seeds. At present, there is following defect in the treatment method that sodium azide induces poinsettia bud change: 1. The concentration (reagent preparation) and processing time that sodium azide effectively induces poinsettia bud change are not clear, how can sodium azide handle poinsettia plant On the problem of effectively causing bud change, there is no research report so far. 2. There is no research report on the si...

Claims

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Application Information

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IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 黄子锋王凤兰郑武贞
Owner 东莞市粮作花卉研究所
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