7 results about "Atropa belladonna" patented technology

This invention discloses a method for refining and desalting belladonna fluid extract, relating to the field of traditional Chinese medicine pharmaceuticals. Belladonna grass is pulverized through a 24-mesh sieve to form coarse powder. The powder is percolated using ethanol as a solvent to obtain a primary belladonna extract. The ethanol is recovered from the primary extract, and the extract is cooled to room temperature. Chlorophyll is removed by separation using a ceramic membrane. The extract is then filtered, and the filtrate is concentrated using an organic nanofiltration membrane to remove salts. The membrane filtrate is further concentrated under reduced pressure to a thick paste. Seven times the volume of ethanol is added and stirred until homogeneous. The mixture is allowed to stand until complete precipitation. The supernatant is collected, and the ethanol is recovered and concentrated to a thick paste. Based on the content determined in the thick paste, an appropriate amount of 85% ethanol is added, and the mixture is diluted with water to prepare a finished belladonna fluid extract product conforming to pharmacopoeia standards. This method is stable and reliable, avoids high-temperature degradation of the active ingredients, effectively removes impurities from the belladonna fluid extract, and prevents salt precipitation in the finished product. This ensures its efficacy and medication safety, and has broad market application prospects.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition with the function of relieving stomach inflammation and a preparation method and application. The traditional Chinese medicine composition comprises a main medicine and an auxiliary medicine, the main medicine consists of cuttle bone, thunberg fritillary bulb and rheum officinale; the adjuvants comprise a first adjuvant and a second adjuvant; the first adjuvant is belladonna and daphniphyllum calycinum; the second adjuvant comprises fructus aurantii and cortex magnoliae officinalis. The traditional Chinese medicine composition provided by the invention is simple in component, only a trace amount of belladonna extract needs to be added, meanwhile, daphniphyllum calycinum is compounded, the addition of excessive other unnecessary components is avoided, and the symptom of chronic non-atrophic gastritis with erosion can be remarkably improved.

This invention discloses the application of overexpression of transcription factor AbbHLH29 in improving the biological traits of belladonna. By overexpressing AbbHLH29 in belladonna, this invention increases the expression level of the H6H gene, iron content, chlorophyll a content, aboveground biomass, or the content of scopolamine and anisodamine. The nucleotide sequence of AbbHLH29 is shown in SEQ ID No. 11. This research is not only significant in elucidating the molecular mechanism by which AbbHLH29 regulates the biosynthesis of belladonna alkaloids and iron homeostasis, but also provides important technical support for future research on belladonna alkaloid metabolic engineering.

The invention discloses a phenyllactic acid UDP-glycosyl transferase mutant and application thereof, and belongs to the technical field of biological medicine, the phenyllactic acid UDP-glycosyl transferase mutant PLA-UGT-M5 with improved catalytic efficiency is obtained through a directed evolution method, the mutant simultaneously contains five amino acid substitutions F19S, G21E, N128S, I209L and I292V, metabolites are detected in yeast cells, and the activity of the metabolites is improved. The catalytic efficiency is obviously higher than that of a wild type. Further functional verification shows that the PLA-UGT-M5 can remarkably promote biosynthesis of the scopolamine and the scopolamine in a yeast cell factory and a belladonna hairy root system, has a good metabolic engineering application prospect, provides a key tool for efficient biosynthesis of the scopolamine and the scopolamine, and has important industrial application potential.

The invention relates to an enteric analgesic suppository for treating haemorrhoids and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine suppositories. The haemorrhoid relieving enteric-coated analgesic suppository is prepared from the following raw materials in parts by weight: 23 to 28 parts of vinegar-processed rhizoma corydalis, 23 to 28 parts of radix angelicae, 2 to 5 parts of borneol, 1 to 2 parts of belladonna extract, 3 to 6 parts of nefopam, 8 to 12 parts of diclofenac sodium, 0.5 to 1 part of meloxicam and 160 to 190 parts of matrix. The traditional Chinese medicines and the western medicines are reasonably configured by analyzing the mechanism of the medicine effect on nerves, the related nerves are influenced from different levels, the mechanism of generating local pain is inhibited or blocked from different angles and different levels, the synergistic analgesia is realized, the obvious analgesic and anti-inflammatory effects are realized, the obvious stimulation to local tissues is avoided, and the safety is good. The suppository analgesic can directly introduce the medicine into an action target position to play a local effect, so that the influence of the medicine on the whole body is reduced.

The invention discloses a sesame bacterial wilt prevention and treatment agent and a prevention and treatment method, and belongs to the technical field of plant protection. The sesame bacterial wilt prevention and treatment agent comprises the following raw materials in parts by mass: 1.5-2 parts of a microbial compound, 1-2 parts of a plant extract, 15-20 parts of a straw fermentation product, 15-20 parts of biomass charcoal, 20-25 parts of peanut shells and 2-3 parts of hydroxypropyl methyl cellulose, wherein the plant extract comprises the following components in parts by mass: 6-9 parts of a nightshade / belladonna mixed extract and 10-12 parts of a purslane extract. The sesame bacterial wilt prevention and control agent has an excellent disease prevention and control effect, and the yield of sesame can be increased while diseases are prevented and controlled.

The application discloses a phenyllactic acid UDP-glycosyltransferase mutant and application thereof, and belongs to the technical field of biological medicine. A phenyllactic acid UDP-glycosyltransferase mutant PLA-UGT-M5 with improved catalytic efficiency is obtained through a directed evolution method. The mutant simultaneously contains five amino acid substitutions F19S, G21E, N128S, I209L and I292V. Metabolic products are detected in yeast cells, and the mutant shows a catalytic efficiency significantly higher than that of a wild type. Further functional verification shows that the PLA-UGT-M5 can significantly promote the biosynthesis of scopolamine and hyoscyamine in a yeast cell factory and a belladonna hairy root system, has a good metabolic engineering application prospect, provides a key tool for the efficient biosynthesis of scopolamine and hyoscyamine, and has important industrial application potential.