This invention discloses a method for refining and desalting belladonna
fluid extract, relating to the field of
traditional Chinese medicine pharmaceuticals. Belladonna grass is pulverized through a 24-mesh
sieve to form coarse
powder. The
powder is percolated using
ethanol as a
solvent to obtain a primary
belladonna extract. The
ethanol is recovered from the primary extract, and the extract is cooled to
room temperature.
Chlorophyll is removed by separation using a
ceramic membrane. The extract is then filtered, and the filtrate is concentrated using an organic
nanofiltration membrane to remove salts. The membrane filtrate is further concentrated under reduced pressure to a thick paste. Seven times the volume of
ethanol is added and stirred until homogeneous. The mixture is allowed to stand until complete
precipitation. The supernatant is collected, and the ethanol is recovered and concentrated to a thick paste. Based on the content determined in the thick paste, an appropriate amount of 85% ethanol is added, and the mixture is diluted with water to prepare a finished belladonna
fluid extract product conforming to
pharmacopoeia standards. This method is stable and reliable, avoids high-temperature degradation of the active ingredients, effectively removes impurities from the belladonna
fluid extract, and prevents salt
precipitation in the finished product. This ensures its
efficacy and medication safety, and has broad market application prospects.