The invention belongs to the technical field of atropa belladonna extract extraction, and concretely relates to a percolation extraction method of atropa belladonna extract. The percolation extractionmethod comprises the steps of medicinal material treatment, wetting, barreling, percolation, and dry extract preparation, and the medicinal material treatment process comprises the steps of taking roots of atropa belladonna, removing impurities and mildewed parts, washing with a saline solution with the mass concentration of 3%, taking out, rubbing with a rubbing machine, and removing non-shredded parts after rubbing to obtain atropa belladonna rubbing shreds; wherein the percolation speed and the solvent temperature are adjusted in the percolation process. Compared with the prior art, the method has the following advantages that the impregnation time and the percolation time can be shortened, and meanwhile, the obtained dry extract can meet the provisions about the belladonna herb dry extract; the extraction time can be shortened by about a half from the traditional 10h, 168-173g of dry extract can be prepared by rubbing every 1000g of atropa belladonna shreds, and the yield of hyoscyamine reaches 85.5% or above.