Medicine for preventing and treating Alzheimer's disease and preparing method thereof

A technology for Alzheimer's disease and medicine, which is applied in the field of medicines for preventing and treating Alzheimer's disease and their preparation, and can solve the problems of separation and identification of active ingredients and reports of pharmacological effects, increasing coronary flow, and anti-virus. and other problems, to achieve the effect of inhibiting Aβ aggregation and toxicity, reducing memory damage, and easy access

Inactive Publication Date: 2009-09-30
THE HONG KONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the second most effective active ingredient of Panax notoginseng, flavonoids have certain effects on lowering blood pressure, increasing coronary flow, and anti-virus, but there are no reports on the isolation, identification and pharmacological effects of its active ingredients, especially those related to estrogen and neuroprotection. Reports about the role

Method used

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  • Medicine for preventing and treating Alzheimer's disease and preparing method thereof
  • Medicine for preventing and treating Alzheimer's disease and preparing method thereof
  • Medicine for preventing and treating Alzheimer's disease and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation and identification of RNFG

[0023] Panax notoginseng was collected in Wenshan, Yunnan Province, and dried naturally. After the crude drug identification, the sample certificate was preserved in the Department of Biology, Hong Kong University of Science and Technology. American ginseng and Korean ginseng were purchased from the Hong Kong market.

[0024] 1 kg of the original medicinal material Radix Notoginseng was pulverized, passed through a 200-mesh sieve, and extracted with 70% ethanol (twice, 3 liters each). The ethanol solution was concentrated in vacuo, the concentrate was heated and dissolved with 500 ml of water, centrifuged for 15 min, the supernatant was taken, passed through a polyamide column, and eluted with water, 30% ethanol and 50% ethanol in sequence, and the 30% ethanol eluted part was dissolved in 100 mL of methanol, as the crude flavonoid fraction. Crude flavonoids were further subjected to Sephadex LH-20 column chromatography...

experiment example 2

[0031] Experimental Example 2: Verification of estrogenic activity of RNFG

[0032] 1. Cell Culture Conditions

[0033] MCF-7 (containing ERE gene) or MCF-7 cells were cultured in MEM medium containing 10% fetal calf serum, penicillin 100U / ml, gentamicin 100ug / ml, 1mM amino acid and 0.1mM sodium pyruvate, Incubator temperature 37°C with 5% CO 2 air and saturation humidity. Three days before the experiment, the medium was changed to phenol red-free MEMα medium containing 2% activated carbon / dextran-treated fetal bovine serum, and other conditions were the same as above.

[0034] 2. Determination of estrogen level

[0035] (1) Determination of Estrogen Response Element-Luciferase Activity (ERE-luciferase)

[0036] MCF-7 cells (containing the ERE gene) were seeded on a 24-well plate with a cell density of about 5 x 10 per well 4 Let the cells adhere to the wall overnight, and add RNFG (1-100 μ M) from the next day, every 24 hours, and replace the culture medium before each a...

experiment example 3

[0039] Experimental example 3: neuroprotective effect of RNFG

[0040] 1. Cell Culture Conditions

[0041] PC12 cells were cultured in DMEM medium, containing 6% fetal bovine serum, containing 6% horse serum, penicillin 100U / ml, gentamicin 100ug / ml, incubator temperature 37°C, containing 7.5% CO 2 air and a certain humidity. The primary cultured cortical nerve cells were obtained by dissecting the cerebral cortex of rat embryos (18 days). The cerebral cortex cells were cultured in neural basal medium containing B27 and 0.5mM GlutaMAX for more than two weeks. The temperature of the incubator was 37°C, containing 5 %CO 2 air and saturation humidity.

[0042] 2. Determination of reactive oxygen species (ROS)

[0043] PC-12 cells were seeded on a 96-well plate at a density of approximately 5 x 10 cells per well 4 Let the cells adhere to the wall overnight, start adding the drug RNFG (1-100μM) the next day, and replace the culture medium before adding the drug. 24 hours after...

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Abstract

The present invention discloses a medicine for preventing and treating Alzheimer's disease. The active ingredient is meletin-3-O-beta-D-xylopyransoyl(1-2)-beta-D-galactopyranoside. The active ingredient RNFG of medicine according to the invention has estrogenic activity, oxidation resisting activity, neuroprotection function for restricting Abeta aggregation and toxicity, and can reduce the memory damage caused by scopolamine. The active ingredient RNFG of medicine according to the invention can be used for preparing the medicine and health product for preventing and treating AD and further has the advantages of usual crude raw material for extracting and easy acquisition.

Description

technical field [0001] The invention belongs to the technical field of medicine and health care, and in particular relates to a medicine for preventing and treating Alzheimer's disease and a preparation method thereof. Background technique [0002] Alzheimer's disease (AD) is a degenerative disease of the central nervous system mainly characterized by progressive cognitive impairment and memory impairment, and dementia is its most prominent mental symptom. Since the etiology and pathogenesis of the disease are not yet clear, it is generally agreed that it is related to the increase of reactive oxygen species and the damage of amyloid protein deposition to nerve cells. Clinically, there is still a lack of effective etiological treatment methods, and the prevention and early detection of AD is even more important. In the middle and late stages, it is mainly nursing. Many drugs have good effects in improving the memory ability, cognitive function and delaying aging of AD pati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P25/28A23L1/30
CPCA61K31/7048A61K36/258A61P25/28
Inventor 詹华强董婷霞
Owner THE HONG KONG UNIV OF SCI & TECH
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