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38 results about "Nojirimycin" patented technology
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Nojirimycin is the parent compound of a class of antibiotics and glycosidase inhibitors. Nojirimycin and its derivatives are mainly obtained from a class of Streptomyces species. Chemically, it is an iminosugar.
Process of extracting 1-deoxy nojirimycin
InactiveCN101020655AGood hypoglycemic effectGuaranteed to bring inOrganic chemistryMetabolism disorderDiabetes modelYerba santa extract
The present invention is process of extracting 1-deoxy nojirimycin (DNJ) from mulberry leaf. Mulberry leaf extract is treated with inorganic acid, cooled to eliminate impurity and column chromatographically separated to obtain DNJ. Animal experiment of mouse shows that the mulberry leaf extract has obvious blood sugar reducing effect, so that the present invention may be used in preparing blood sugar reducing health product. The present invention has low preparation cost, and is suitable for industrial production.
Owner:ZHEJIANG UNIV
Preparation method of ultramicro mulberry leaf tea powder
InactiveCN101797000AGive full play to health careGive full play to nutritional valueTea substituesNojirimycinAlpha-Glucosidases
The invention relates to the preparation of tea and aims to provide a preparation method of ultramicro mulberry leaf tea powder. For obtaining ultramicro mulberry leaf powder and ultramicro tea powder, the method comprises the following steps of: selecting raw materials, killing out, kneading, drying, primarily crushing and ultromicro crushing; sufficiently mixing ultramicro mulberry leaf powder with a nojirimycin crude product with a V type mixer, and sufficiently mixing the mixture with ultramicro tea powder to obtain the ultromicro mulberry leaf tea powder, wherein the weight content of nojirimycin in the nojirimycin crude product is 10-12 percent; and finally packing. For the ultromicro mulberry leaf tea prepared by the invention, ultromicro crushed to obtain the grain size less than 10 mum, the powder can be sufficiently suspended in hot water and can be completely taken, thereby the health care and nutritious value of the mulberry leaf are fully exerted. The nojirimycin crude product is added in the ultramicro mulberry leaf tea powder so that the activity of alpha-glucosidase can be more effectively restrained, glucose entering the blood is reduced, and the blood sugar is reduced. The invention can be used for health care or auxiliarily treating diabetes.
Owner:ZHEJIANG UNIV
Method for separating 1-deoxidized nojirimycin monomer from mulberry leaf total alkaloid
The invention relates to the technology field of effective ingredient separation of the Chinese traditional medicine, particularly a method for separating 1-desoxy nojirimycin monomer from total alkaloid of mulberry leaf. The method comprises the steps as follows: preparing a sample solution; pretreating adsorbent and packing into a column; passing through the resin column at the room temperature; collecting eluates part by part; subjecting to the qualitative detection; mixing the eluates with the same composition; concentrating under the reduced pressure; vacuum-drying; and recrystallizing to obtain 1-desoxy nojirimycin crystals. According to the high-efficiency liquid chromatography measurement, the purity of the crystal is higher than 95% and the yield of the crystal is higher than 75%.
Owner:JIANGSU UNIV
Sanggenone C and sanggenone D extracted from morus plants and new medicine application of composition
The invention discloses sanggenone C and sanggenone D extracted from white mulberry root-barks, mulberry twigs and folium leaves and a new medicine application of a composition comprising the sanggenone C and the sanggenone D which are main ingredients, particularly the application of the single ingredient sanggenone C or sanggenone D and the composition composed of the sanggenone C, the sanggenone D, mulberrin, mulberroside, oxidized resveratrol, resveratrol and deoxynojirimycin in preparation of alpha-glucosidase inhibitor medicines. The composition is prepared by respectively extracting medicinal materials containing the chemical components above and blending according to certain proportions, or using several extraction methods for extracting the medicinal materials simultaneously containing the chemical components above. The white mulberry root-barks, the mulberry twigs and the folium leaves are accidentally found to contain the sanggenone C and the sanggenone D apart from containing alkaloids such as deoxynojirimycin; and the sanggenone C and the sanggenone D have stronger pharmacological activity than the alkaloids such as deoxynojirimycin.
Owner:赵全成
A kind of deoxynojirimycin extraction method
InactiveCN102276515AReduce energy consumptionSave energyOrganic chemistryDeoxynojirimycineReverse osmosis
The invention discloses a method for extracting a deoxynojirimycin monomer. The method comprises the following steps of: soaking a mulberry raw material in acidic aqueous solution, and extracting for 1 to 3 times; adsorbing extracting solution on a cation exchange resin, washing, and eluting by using ammonia water; performing reverse osmosis pre-concentration on eluent, recovering ammonia, dissolving an intermediate in a hydrophilic solvent, performing catalytic conversion, allowing the solution to pass through a gel chromatographic column, and eluting the hydrophilic solvent; recovering the solvent, dissolving in a mixed solvent, allowing the mixed solvent to pass through the chromatographic column, and eluting the mixed solvent; and recovering the mixed solution to obtain pure deoxynojirimycin products. The method is low in energy consumption and environment-friendly, and the pure deoxynojirimycin products with various purity grades of 10 percent, 50 percent, 99 percent and the like.
Owner:SOUTHWEST UNIV
Edible composition
InactiveUS20140227371A1Inhibit lipid accumulationImpaired liver functionBiocideInorganic active ingredientsLiver functionNormal liver function
The present invention relates to edible compositions for the maintenance of normal liver function comprising zinc, 1-deoxynojirimycinand α-lipoic acid.
Owner:CONOPCO INC D B A UNILEVER
Preparation method of high-purity 1-deoxynojirimycin
ActiveCN111269171AHigh quality contentMethod environmentally friendlyOrganic chemistryAgainst vector-borne diseasesHigh concentrationDeoxynojirimycine
The invention discloses a preparation method of high-purity 1-deoxynojirimycin. 1-deoxynojirimycin is enriched by using ammonium cation exchange resin, and the 1-deoxynojirimycin is pulped and purified by combining anion exchange resin and a hot solvent to prepare the high-purity 1-deoxynojirimycin. The use of a high-concentration acid liquor can be avoided, so that equipment in a production workshop is prevented from being corroded by acids, the production risk is reduced, the energy consumption can be reduced, the solvent loss can be reduced, the product yield can be increased, and finally,the product with the mass content of 1-deoxynojirimycin being greater than 98.0% can be prepared.
Owner:劲牌持正堂药业有限公司
Application of 1-deoxynojirimycin in treatment of diabetic cardiomyopathy and liver injury
PendingCN110368388AExpand the field of medicineReduces levels of oxidative stress in the heartMetabolism disorderDigestive systemMyocardial fiberAlpha-glucosidase inhibitor
The invention relates to application of 1-deoxynojirimycin in the treatment of diabetic cardiomyopathy and liver injury. 1-Deoxynojirimycin is a known potent alpha-glucosidase inhibitor. The applicable field of 1-deoxynojirimycin is widened herein; the use of 1-deoxynojirimycin or its pharmaceutically acceptable salts in the treatment and / or prevention of diabetic cardiomyopathy and liver injury is provided for the first time; experiments show that 1-deoxynojirimycin plays a positive role in myocardial protection aspects, such as lowering cardiac oxidative stress level of a diabetic patient, inhibiting cardiovascular endothelial cell proliferation of the diabetic patient, and inhibiting myocardial fiber proliferation of the diabetic patient; it is expected to develop 1-deoxynojirimycin into drugs to treat diabetic cardiomyopathy, thereby filling the blank of the prior art.
Owner:陕西含光生物科技有限公司
Method for extracting and preparing N-methyl-1-deoxy noijirimycin
The method of extracting and preparing N-methyl-1-deoxy nojirimycin includes the following steps: A. crushing mulberry plant material, soaking in water, filtering and concentrating filtrate to obtain concentrate; B. precipitating the concentrate with alcohol, separating the supernatant with cationic exchange resin, water washing, and eluting with 0.2-0.5 M concentration ammonia solution; C. collecting concentrating the ammonia solution eluted liquid; D. adding formaldehyde and acetic acid or formic acid to the concentrate of the last step, maintaining at 40-90 deg c for 1-10 hr and separating with cationic exchange resin to obtain N-methyl-1-deoxy nojirimycin. The method has simple technological process and high yield, and is suitable for industrial production.
Owner:车庆明
Preparation method of mulberry leaf extract containing 1-deoxynojirimycin
ActiveCN105294542ASimple and fast operationHigh yieldOrganic chemistryBiotechnologyDeoxynojirimycine
The invention provides a preparation method of mulberry leaf extract containing 1-deoxynojirimycin. The method mainly including the steps of saline solution water-proof cooking and softening, enzymolysis, polyamide column purification and simulated moving bed refining is determined by sufficiently considering the raw material characteristics of mulberry leaves and the dissolution characteristics of the target component 1-deoxynojirimycin and conducting system screening and optimizing. The separation effect is extremely good, and the concentration of 1-deoxynojirimycin in the finally obtained mulberry leaf extract is larger than 95 wt%. All materials used for the whole method are conventional materials, cost is low, yield is high, solvent can be recycled, environment pollution can be reduced, and industrialization can be easily achieved.
Owner:内蒙古昶辉生物科技股份有限公司
Method for preparing larva powder of domestic silkworm with high content of 1 - deoxidized nojirimycin
InactiveCN101091724AHigh content of 1-deoxynojirimycinGood hypoglycemic effectPowder deliveryAnthropod material medical ingredientsMedicineNojirimycin
The present invention discloses a domestic silkworm larva powder with high 1-deoxynojirimycin (DNj) content and its preparation method. Said domestic silkworm larva powder not only has high 1-deoxynojirimycin content, but also has the obvious effect for reducing blood sugar, so that it can be made into the medicine and health-care food for preventing and curing diabetes. Besides, said invention also provides the concrete steps of its preparation method.
Owner:ZHEJIANG UNIV
Bacillus amyloliquefaciens and application thereof in preparation of 1-deoxynojirimycin
ActiveCN112094789AEnhanced inhibitory effectIncrease contentBacteriaMicroorganism based processesBiotechnologyDeoxynojirimycine
The invention discloses bacillus amyloliquefaciens capable of producing 1-deoxynojirimycin at high yield. The strain preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No. 19098. The invention also discloses a method for producing the 1-deoxynojirimycin by fermenting the bacillus amyloliquefaciens, and the yield of the 1-deoxynojirimycinreaches 0.9 g / L. The 1-deoxynojirimycin has alpha-glucosidase inhibitory activity and can be applied to blood glucose control of diabetics, so that the strain and the method for preparing the 1-deoxynojirimycin through fermentation of the strain have wide application prospects.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI +1
Synthesis of nojirimycins
InactiveUS20070015793A1Reduce wasteIncrease productionBiocideOrganic chemistryDeoxynojirimycineNojirimycin
Disclosed are methods of preparing substituted nojirimycins represented by the following formula, comprising reacting 1-deoxynojirimycin with a reagent prepared by reacting an oxidizing agent with the compound represented by the following formula.
Owner:MACROZYME DNM
Oral preparation for slowing down absorption of alpha-glycosidase inhibitor and enhancing hypoglycemic drug effect
ActiveCN102210866AReduce absorptionProlong the action timeMetabolism disorderMacromolecular non-active ingredientsDiseaseCellulose
The invention discloses an oral preparation for slowing down the absorption of an alpha-glycosidase inhibitor and enhancing a hypoglycemic drug effect. The preparation is an oral preparation prepared by adding the following adhesive auxiliary materials based on the certain proportion: carboxymethyl cellulose, hydroxyethyl cellulose, methyl cellulose or beta-cyclodextrin and the like, to each alpha-glycosidase inhibitor (acarbose, voglibose, miglitol, 1-deoxynojirimycin and extract of the 1-deoxynojirimycin). Compared with singly taken alpha-glycosidase inhibitors, the adhesives can be used for prolonging the action time of the alpha-glycosidase inhibitor in the intestine, slowing down the absorption of a medicament and glucose in the intestine and further reducing a plasma concentration, thus the hypoglycemic drug effect is improved. The oral preparation disclosed by the invention can be applied to the prevention and therapy of diseases, such as diabetes, obesity and the like.
Owner:NANKAI UNIV +1
Method for preparing miglitol midbody N-substituted-1-deoxidization nojirimycin derivative
InactiveCN101270378AReduce lossesLarge amount of processingMicroorganism based processesOn/in organic carrierMiglitolNojirimycin
The invention relates to a preparation method of miglitol intermediate, in particular to a preparation method of a derivative of N-substitution-1-deoxynojirimycin which is the key intermediate of miglitol. Gluoconobacter oxydans or the Gluoconobacter oxydans treated by immobilized cell technology are used repeatedly for transforming the substrate to the derivative of N-substitution-1-deoxynojirimycin which is the miglitol intermediate, the transfer temperature is controlled at 5 to 25 DEG C, pH value is controlled at 4.0 to 6.5 and dissolved oxygen is controlled at 5 to 80 percent (in volume). Adopting the invention can decrease the biotransformation cost, reduce the inter-assay of cell, and be suitable for the large scale industrial production, increase the mechanical strength of the wall of the strain prepared, make the strain not to dissolve during the cultivation in long-term liquid environment, lengthen the service life and have outstanding slow release performance.
Owner:ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
Process of extracting 1-deoxy nojirimycin from mulberry leaf
InactiveCN100556892CGood hypoglycemic effectGuaranteed to bring inOrganic chemistryMetabolism disorderDiabetes modelIon exchange
Owner:ZHEJIANG UNIV
Method for detecting content of alpha-homonojirimycin in suregade glomerulata medicinal materials
ActiveCN108828121AStrong alpha-glucosidase inhibitory activityIncrease contentComponent separationAcetonitrileChloroform
The invention relates to the field of traditional Chinese medicine content detection, in particular to a method for detecting content of alpha-homonojirimycin in suregade glomerulata medicinal materials. According to the method, alpha-homonojirimycin is taken as a control, acyl chloride derivatization reagents are used for precolumn derivatization of alpha-homonojirimycin, dichloromethane or chloroform is added to accelerate the reaction, an AgilentExtendC18 chromatographic column is used as the chromatographic column, and the mixture of methanol, acetonitrile and water is used as the mobile phase, and separating determination and quantitative analysis of alpha-homonojirimycin is conducted with high performance liquid chromatography. The detection method for the suregade glomerulata medicinal materials is quick, accurate and convenient.
Owner:CHINA RESOURCES SANJIU MEDICAL & PHARMA +1
Effective-component extract of Foliun-Mori and its preparation method
InactiveCN1259915CLow calorieGood blood pressureSilicon organic compoundsMetabolism disorderAlcoholMedicine
The invention relates to a mulberry leaf effective ingredient extract and its manufacturing method. The extract is deposited out from the water extracting liquid of the mulberry leaf, and then carries on alcohol sediment; the nojirimycin in it is 1.0-3.0%, gamma-aminobutyric acid 0.8-1.5%. The purity of the extract can be upgraded greatly, the effective ingredient will be upgraded prominently, and it can be applied to product food, healthy keeping product and medicine for reducing blood sugar, controlling blood pressure, and improving the intestinal canal.
Owner:上海润巍投资管理有限公司
Preparation method and application of healthy food base material capable of improving metabolic disturbance of glycolipid
ActiveCN107836707AHigh activityIncrease initial gamma-aminobutyric acid contentNatural extract food ingredientsFood ingredient functionsNojirimycinGlycolipid
The invention discloses a preparation method and application of a healthy food base material capable of improving metabolic disturbance of glycolipid. The preparation method comprises the following steps of picking mulberry leaves, and performing pretreatment; preparing fresh mulberry leaf virgin pulp; preparing mulberry leaf acidic polysaccharose; preparing a tartarian buckwheat extract; preparing an oat extract; preparing composite virgin pulp; performing sterilization and fermentation; and performing drying, packaging and sterilization. The healthy food base material is rich in gamma-aminobutyric acid, N-saccharide compounds (1-deoxidized nojirimycin, fagopyrin and the like), micromolecular oligosaccharide prebiotics and probiotics. According to the method, on one hand, the content of the gamma-aminobutyric acid and the content of the N-saccharide compounds in raw materials are increased through optimizing microorganism fermentation strains and a fermentation technology, besides, conversion of natural macromolecular polysaccharide in the raw materials into the oligosaccharide prebiotics can also be promoted in the fermentation process, and on the other hand, the bioactivity of functional factors of the gamma-aminobutyric acid, the N-saccharide compounds, the micromolecular oligosaccharide prebiotics, the probiotics and the like in products can be reserved to the maximum extent through optimizing a preparation technology.
Owner:山东夏津古桑产业研究院有限公司
1-deoxynojirimycin enriched mulberry leaf preparation method and obtained ultra-fine powder
ActiveCN105520152AImprove absorption and utilizationFit for consumptionFood ingredient functionsUltra fineD-Glucose
The present invention relates to a 1-deoxynojirimycin enriched mulberry leaf preparation method and an obtained ultra-fine powder, which belongs to the processing field of medicinal and edible food. Fresh and complete tender mulberry leaves are washed, air-dried and cut into slice shapes with a length and width of 3-6 cm*3-6 cm, the mulberry leaf broken slices are soaked in a glucose solution at a temperature of 30-60 DEG C and a concentration of 10-30 g / L for 3-5 hours, the taken mulberry broken slices are dried to a water content to below 5%, the dried mulberry broken slices are crushed into an average particle size of 8 [mu]m or less, and the crushed mulberry leaves are dried to a water content to below 5%, thereby obtaining the 1-deoxynojirimycin enriched mulberry leaf ultra-fine powder. During the fresh mulberry leaf soaking process, endogenous DNJ synthesis related enzymes are activated and the glucose is catalyzed to enrich DNJ. The highest content of the finished product of the DNJ is 250mg / 100g. The obtained product by the method is clear in health-care function and safe and reliable in consumption.
Owner:镇江市智农食品有限公司
1-deoxynojirimycin-hydroxychalcone hybrid derivative and its preparation method and application
ActiveCN109456254BLower postprandial blood sugarStrong inhibitory activityOrganic chemistryMetabolism disorderAlgluceraseDeoxynojirimycine
Owner:JIANGXI AGRICULTURAL UNIVERSITY
Method for detecting content of alpha-homonojirimycin in suregade glomerulata medicinal material
ActiveCN111024868AAchieve direct qualitativeRealize quantitative analysisComponent separationMedicinal herbsResolution (mass spectrometry)
The invention provides a method for detecting the content of alpha-homonojirimycin in a suregade glomerulata medicinal material. The method comprises the following steps: preparing a reference substance solution, namely, precisely weighing an alpha-nojirimycin reference substance, and adding a solvent for dissolving to obtain the reference substance solution with required concentration; preparinga test solution, namely, taking suregade glomerulata medicinal material powder to be detected, adding an extraction solvent for extracting, carrying out centrifuging, taking a supernatant, and fixingthe volume to obtain the test solution; and carrying out content determination, namely, determining the peak areas of the alpha-homonojirimycin in the reference substance solution and the test solution through a liquid chromatography-tandem mass spectrometry method, and calculating the content of the alpha-homonojirimycin in the suregade glomerulata medicinal material according to an external standard method. According to the method, a liquid chromatography-tandem mass spectrometry method is adopted, resolution of the polyhydroxy alkaloid substance alpha-homonojirimycin and the four chiral isomers of the polyhydroxy alkaloid substance alpha-homonojirimycin is achieved for the first time, direct qualitative and quantitative analysis of the alpha-homonojirimycin is achieved, and the method has good system applicability, stability, repeatability and reproducibility.
Owner:CHINA RESOURCES SANJIU MEDICAL & PHARMA
1-Deoxynojirimycin-methyl hydroxycinnamate hybrid derivative and preparation method and application thereof
ActiveCN109293564ALower postprandial blood sugarLower blood sugar regulationOrganic chemistryMetabolism disorderDigestive enzymeAlpha-glucosidase inhibitor
The invention relates to the field of medicine and food, in particular to 1-deoxynojirimycin-methyl hydroxycinnamate hybrid derivative and a preparation method and application thereof. The 1-deoxynojirimycin-methyl hydroxycinnamate hybrid derivative has a structure shown as formula (I); in-vitro text proves that the 1-deoxynojirimycin-methyl hydroxycinnamate hybrid derivative is an efficient alpha-glucosidase inhibitor evidently more active in inhibiting alpha-glucosidase than the post-diet glucose controller, acarbose, the most common at present. As the 1-deoxynojirimycin-methyl hydroxycinnamate hybrid derivative can efficiently inhibit activity of sugar digestive enzymes, such as alpha-glucosidase and effectively lower post-diet glucose, the 1-deoxynojirimycin-methyl hydroxycinnamate hybrid derivative has a promising application prospect in hypoglycemic drugs and auxiliary hypoglycemic foods.
Owner:JIANGXI AGRICULTURAL UNIVERSITY
Method for extracting deoxynojirimycin
InactiveCN102276515BReduce energy consumptionSave energyOrganic chemistryDeoxynojirimycineReverse osmosis
The invention discloses a method for extracting a deoxynojirimycin monomer. The method comprises the following steps of: soaking a mulberry raw material in acidic aqueous solution, and extracting for 1 to 3 times; adsorbing extracting solution on a cation exchange resin, washing, and eluting by using ammonia water; performing reverse osmosis pre-concentration on eluent, recovering ammonia, dissolving an intermediate in a hydrophilic solvent, performing catalytic conversion, allowing the solution to pass through a gel chromatographic column, and eluting the hydrophilic solvent; recovering the solvent, dissolving in a mixed solvent, allowing the mixed solvent to pass through the chromatographic column, and eluting the mixed solvent; and recovering the mixed solution to obtain pure deoxynojirimycin products. The method is low in energy consumption and environment-friendly, and the pure deoxynojirimycin products with various purity grades of 10 percent, 50 percent, 99 percent and the like.
Owner:SOUTHWEST UNIV
Preparation method of ultramicro mulberry leaf tea powder
The invention relates to the preparation of tea and aims to provide a preparation method of ultramicro mulberry leaf tea powder. For obtaining ultramicro mulberry leaf powder and ultramicro tea powder, the method comprises the following steps of: selecting raw materials, killing out, kneading, drying, primarily crushing and ultromicro crushing; sufficiently mixing ultramicro mulberry leaf powder with a nojirimycin crude product with a V type mixer, and sufficiently mixing the mixture with ultramicro tea powder to obtain the ultromicro mulberry leaf tea powder, wherein the weight content of nojirimycin in the nojirimycin crude product is 10-12 percent; and finally packing. For the ultromicro mulberry leaf tea prepared by the invention, ultromicro crushed to obtain the grain size less than 10 mum, the powder can be sufficiently suspended in hot water and can be completely taken, thereby the health care and nutritious value of the mulberry leaf are fully exerted. The nojirimycin crude product is added in the ultramicro mulberry leaf tea powder so that the activity of alpha-glucosidase can be more effectively restrained, glucose entering the blood is reduced, and the blood sugar is reduced. The invention can be used for health care or auxiliarily treating diabetes.
Owner:ZHEJIANG UNIV
A method for separating trace alkaloid monomers from total alkaloids of Cortex Morus alba
The invention relates to a technical method for separating monomers with hypoglycemic alkaloid activity such as fagopyrin, 1,4-dideoxy-1,4-imino-d-arabinitol and 1-deoxynojirimycin from white mulberry root-bark total alkaloid. The method comprises the following steps: by using the self-extracted white mulberry root-bark total alkaloid as a primary material, re-dissolving through methyl alcohol to remove most of DNJ (1-deoxynojirimycin); concentrating eluent, then performing derivatization reaction, performing column chromatography through a silica gel column, obtaining multiple active alkaloid monomers through column elution, and finally, dissociating derivatized groups to obtain the alkaloid monomers. The content of the obtained monomers exceeds 95.0 percent, and the monomers can be used as standard reference materials or other materials.
Owner:HEFEI HUAFANG PHARMA SCI & TECH
Method for separating 1-deoxidized nojirimycin monomer from mulberry leaf total alkaloid
The invention relates to the technology field of effective ingredient separation of the Chinese traditional medicine, particularly a method for separating 1-desoxy nojirimycin monomer from total alkaloid of mulberry leaf. The method comprises the steps as follows: preparing a sample solution; pretreating adsorbent and packing into a column; passing through the resin column at the room temperature; collecting eluates part by part; subjecting to the qualitative detection; mixing the eluates with the same composition; concentrating under the reduced pressure; vacuum-drying; and recrystallizing to obtain 1-desoxy nojirimycin crystals. According to the high-efficiency liquid chromatography measurement, the purity of the crystal is higher than 95% and the yield of the crystal is higher than 75%.
Owner:JIANGSU UNIV
Method for preparing miglitol midbody N-substituted-1-deoxidization nojirimycin derivative
InactiveCN101270378BReduce lossesLarge amount of processingMicroorganism based processesOn/in organic carrierMiglitolOxygen
The invention relates to a preparation method of miglitol intermediate, in particular to a preparation method of a derivative of N-substitution-1-deoxynojirimycin which is the key intermediate of miglitol. Gluoconobacter oxydans or the Gluoconobacter oxydans treated by immobilized cell technology are used repeatedly for transforming the substrate to the derivative of N-substitution-1-deoxynojirimycin which is the miglitol intermediate, the transfer temperature is controlled at 5 to 25 DEG C, pH value is controlled at 4.0 to 6.5 and dissolved oxygen is controlled at 5 to 80 percent (in volume). Adopting the invention can decrease the biotransformation cost, reduce the inter-assay of cell, and be suitable for the large scale industrial production, increase the mechanical strength of the wall of the strain prepared, make the strain not to dissolve during the cultivation in long-term liquid environment, lengthen the service life and have outstanding slow release performance.
Owner:ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
Method of altering glycosylation of proteins in response to nojirimycin glucuronide in a plant cell expressing glucuronidase
Novel pro-drugs and methods for their use to alter the growth and biological characteristics of living cells, tissues, or whole organisms are described. Such cells may include animal cells, plant cells and bacterial cells. The methods allow for selective activation of the pro-drugs at or near transformant host cells expressing a gene for an enzyme that activates the pro-drugs. Pro-drugs according to a preferred embodiment of the invention are conjugates of a bioactive compound and a chemical group that is capable of being cleaved from the bioactive compound by action of an enzyme. Methods according to this invention include, (a) introducing into targeted cells a gene encoding an enzyme and (b) administering a pro-drug, wherein the enzyme releases the pro-drug from conjugation. In a preferred embodiment of the invention, the gene encoding the enzyme is a marker gene. Methods according to a preferred embodiment of the invention include altering protein production in a plant cell such as altering glycosylation of a protein produced by the plant cell.
Owner:MARKER GENE TECH
Preparation method and application of a healthy food base material capable of improving glucose and lipid metabolism disorders
ActiveCN107836707BHigh activityIncrease initial gamma-aminobutyric acid contentNatural extract food ingredientsFood ingredient functionsBiotechnologyPolygonum fagopyrum
The invention discloses a preparation method and application of a health food base material capable of improving glucose and lipid metabolism disorders, comprising the following steps: picking and pretreatment of mulberry leaves; preparation of fresh mulberry leaf puree; preparation of mulberry leaf acidic polysaccharide; preparation of tartary buckwheat extract ; Preparation of oat extract; Preparation of compound puree; Sterilization and fermentation; Drying, packaging and sterilization. The health food base material of this invention is rich in γ-aminobutyric acid, N-saccharide compounds (1-deoxynojirimycin and buckwheat alkali, etc.), small molecule oligosaccharide prebiotics and probiotics; Optimize the microbial fermentation strain and fermentation process to increase the content of γ-aminobutyric acid and N-saccharide compounds in the raw material, and at the same time promote the conversion of natural macromolecular polysaccharides in the raw material into oligosaccharide prebiotics during the fermentation process. On the one hand, the biological activity of functional factors such as γ-aminobutyric acid, N-saccharide compounds, small molecular oligosaccharides, prebiotics and probiotics can be retained to the greatest extent through optimization of the preparation process.
Owner:山东夏津古桑产业研究院有限公司
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