36 results about "Nojirimycin" patented technology

The present invention is process of extracting 1-deoxy nojirimycin (DNJ) from mulberry leaf. Mulberry leaf extract is treated with inorganic acid, cooled to eliminate impurity and column chromatographically separated to obtain DNJ. Animal experiment of mouse shows that the mulberry leaf extract has obvious blood sugar reducing effect, so that the present invention may be used in preparing blood sugar reducing health product. The present invention has low preparation cost, and is suitable for industrial production.

The invention relates to the preparation of tea and aims to provide a preparation method of ultramicro mulberry leaf tea powder. For obtaining ultramicro mulberry leaf powder and ultramicro tea powder, the method comprises the following steps of: selecting raw materials, killing out, kneading, drying, primarily crushing and ultromicro crushing; sufficiently mixing ultramicro mulberry leaf powder with a nojirimycin crude product with a V type mixer, and sufficiently mixing the mixture with ultramicro tea powder to obtain the ultromicro mulberry leaf tea powder, wherein the weight content of nojirimycin in the nojirimycin crude product is 10-12 percent; and finally packing. For the ultromicro mulberry leaf tea prepared by the invention, ultromicro crushed to obtain the grain size less than 10 mum, the powder can be sufficiently suspended in hot water and can be completely taken, thereby the health care and nutritious value of the mulberry leaf are fully exerted. The nojirimycin crude product is added in the ultramicro mulberry leaf tea powder so that the activity of alpha-glucosidase can be more effectively restrained, glucose entering the blood is reduced, and the blood sugar is reduced. The invention can be used for health care or auxiliarily treating diabetes.

The invention relates to the technology field of effective ingredient separation of the Chinese traditional medicine, particularly a method for separating 1-desoxy nojirimycin monomer from total alkaloid of mulberry leaf. The method comprises the steps as follows: preparing a sample solution; pretreating adsorbent and packing into a column; passing through the resin column at the room temperature; collecting eluates part by part; subjecting to the qualitative detection; mixing the eluates with the same composition; concentrating under the reduced pressure; vacuum-drying; and recrystallizing to obtain 1-desoxy nojirimycin crystals. According to the high-efficiency liquid chromatography measurement, the purity of the crystal is higher than 95% and the yield of the crystal is higher than 75%.

The invention discloses sanggenone C and sanggenone D extracted from white mulberry root-barks, mulberry twigs and folium leaves and a new medicine application of a composition comprising the sanggenone C and the sanggenone D which are main ingredients, particularly the application of the single ingredient sanggenone C or sanggenone D and the composition composed of the sanggenone C, the sanggenone D, mulberrin, mulberroside, oxidized resveratrol, resveratrol and deoxynojirimycin in preparation of alpha-glucosidase inhibitor medicines. The composition is prepared by respectively extracting medicinal materials containing the chemical components above and blending according to certain proportions, or using several extraction methods for extracting the medicinal materials simultaneously containing the chemical components above. The white mulberry root-barks, the mulberry twigs and the folium leaves are accidentally found to contain the sanggenone C and the sanggenone D apart from containing alkaloids such as deoxynojirimycin; and the sanggenone C and the sanggenone D have stronger pharmacological activity than the alkaloids such as deoxynojirimycin.

The present invention relates to edible compositions for the maintenance of normal liver function comprising zinc, 1-deoxynojirimycinand α-lipoic acid.

The invention discloses a preparation method of high-purity 1-deoxynojirimycin. 1-deoxynojirimycin is enriched by using ammonium cation exchange resin, and the 1-deoxynojirimycin is pulped and purified by combining anion exchange resin and a hot solvent to prepare the high-purity 1-deoxynojirimycin. The use of a high-concentration acid liquor can be avoided, so that equipment in a production workshop is prevented from being corroded by acids, the production risk is reduced, the energy consumption can be reduced, the solvent loss can be reduced, the product yield can be increased, and finally,the product with the mass content of 1-deoxynojirimycin being greater than 98.0% can be prepared.

The method of extracting and preparing N-methyl-1-deoxy nojirimycin includes the following steps: A. crushing mulberry plant material, soaking in water, filtering and concentrating filtrate to obtain concentrate; B. precipitating the concentrate with alcohol, separating the supernatant with cationic exchange resin, water washing, and eluting with 0.2-0.5 M concentration ammonia solution; C. collecting concentrating the ammonia solution eluted liquid; D. adding formaldehyde and acetic acid or formic acid to the concentrate of the last step, maintaining at 40-90 deg c for 1-10 hr and separating with cationic exchange resin to obtain N-methyl-1-deoxy nojirimycin. The method has simple technological process and high yield, and is suitable for industrial production.

The present invention discloses a domestic silkworm larva powder with high 1-deoxynojirimycin (DNj) content and its preparation method. Said domestic silkworm larva powder not only has high 1-deoxynojirimycin content, but also has the obvious effect for reducing blood sugar, so that it can be made into the medicine and health-care food for preventing and curing diabetes. Besides, said invention also provides the concrete steps of its preparation method.

The invention discloses bacillus amyloliquefaciens capable of producing 1-deoxynojirimycin at high yield. The strain preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No. 19098. The invention also discloses a method for producing the 1-deoxynojirimycin by fermenting the bacillus amyloliquefaciens, and the yield of the 1-deoxynojirimycinreaches 0.9 g/L. The 1-deoxynojirimycin has alpha-glucosidase inhibitory activity and can be applied to blood glucose control of diabetics, so that the strain and the method for preparing the 1-deoxynojirimycin through fermentation of the strain have wide application prospects.

Disclosed are methods of preparing substituted nojirimycins represented by the following formula,

comprising reacting 1-deoxynojirimycin with a reagent prepared by reacting an oxidizing agent with the compound represented by the following formula.

The invention relates to a preparation method of miglitol intermediate, in particular to a preparation method of a derivative of N-substitution-1-deoxynojirimycin which is the key intermediate of miglitol. Gluoconobacter oxydans or the Gluoconobacter oxydans treated by immobilized cell technology are used repeatedly for transforming the substrate to the derivative of N-substitution-1-deoxynojirimycin which is the miglitol intermediate, the transfer temperature is controlled at 5 to 25 DEG C, pH value is controlled at 4.0 to 6.5 and dissolved oxygen is controlled at 5 to 80 percent (in volume). Adopting the invention can decrease the biotransformation cost, reduce the inter-assay of cell, and be suitable for the large scale industrial production, increase the mechanical strength of the wall of the strain prepared, make the strain not to dissolve during the cultivation in long-term liquid environment, lengthen the service life and have outstanding slow release performance.

The invention relates to the field of traditional Chinese medicine content detection, in particular to a method for detecting content of alpha-homonojirimycin in suregade glomerulata medicinal materials. According to the method, alpha-homonojirimycin is taken as a control, acyl chloride derivatization reagents are used for precolumn derivatization of alpha-homonojirimycin, dichloromethane or chloroform is added to accelerate the reaction, an AgilentExtendC18 chromatographic column is used as the chromatographic column, and the mixture of methanol, acetonitrile and water is used as the mobile phase, and separating determination and quantitative analysis of alpha-homonojirimycin is conducted with high performance liquid chromatography. The detection method for the suregade glomerulata medicinal materials is quick, accurate and convenient.

The invention relates to a mulberry leaf effective ingredient extract and its manufacturing method. The extract is deposited out from the water extracting liquid of the mulberry leaf, and then carries on alcohol sediment; the nojirimycin in it is 1.0-3.0%, gamma-aminobutyric acid 0.8-1.5%. The purity of the extract can be upgraded greatly, the effective ingredient will be upgraded prominently, and it can be applied to product food, healthy keeping product and medicine for reducing blood sugar, controlling blood pressure, and improving the intestinal canal.

The invention relates to the field of medicine and food, in particular to 1-deoxynojirimycin-methyl hydroxycinnamate hybrid derivative and a preparation method and application thereof. The 1-deoxynojirimycin-methyl hydroxycinnamate hybrid derivative has a structure shown as formula (I); in-vitro text proves that the 1-deoxynojirimycin-methyl hydroxycinnamate hybrid derivative is an efficient alpha-glucosidase inhibitor evidently more active in inhibiting alpha-glucosidase than the post-diet glucose controller, acarbose, the most common at present. As the 1-deoxynojirimycin-methyl hydroxycinnamate hybrid derivative can efficiently inhibit activity of sugar digestive enzymes, such as alpha-glucosidase and effectively lower post-diet glucose, the 1-deoxynojirimycin-methyl hydroxycinnamate hybrid derivative has a promising application prospect in hypoglycemic drugs and auxiliary hypoglycemic foods.