The application discloses a method for in-situ encapsulating
enzyme by
lignin. The pH of industrial
lignin water dispersion is adjusted to above 10.0 to dissolve the industrial
lignin, and the industrial lignin solution is obtained after filtering and removing impurities. Then the pH of the industrial lignin solution is adjusted to 5-9.5, and
enzyme protein is added to obtain a
mixed solution. The pH of the
mixed solution is adjusted to 3.0-4.0 again, so that the industrial lignin and the
enzyme protein are co-precipitated. After separating the precipitate,
drying is performed to obtain the
immobilized enzyme. The application uses the adsorption capacity of lignin to enzyme and the property of alkali-soluble acid-extractable of lignin to immobilize the enzyme with low pH sensitivity, so that the encapsulation rate of the enzyme is improved, and the activity of the
immobilized enzyme is better preserved. The application provides a new method for in-situ encapsulating
enzyme protein, and has a positive significance for promoting the development of lignocellulose bio-refining and green bio-manufacturing.