Method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme

A honeysuckle leaf, catalytic activation technology, applied in organic chemistry, separation/purification of carboxylic acid esters, etc., can solve the problem of chlorogenic acid extraction and purification process instability, affecting the economic development value of chlorogenic acid products, and increasing production energy consumption. and cost issues, to achieve the effect of ensuring extraction energy efficiency and product quality, reducing environmental treatment costs, and low production costs

Inactive Publication Date: 2013-05-08
王星敏
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main disadvantages of this method are: (1) the method adopts directly soaking honeysuckle leaves and eluting the soaking liquid to obtain chlorogenic acid, because the solubility of chlorogenic acid in water at 25°C is about 4%, and the chlorogenic acid in honeysuckle leaves at normal temperature The amount of acid soluble in soaking liquid is few, causes the waste of chlorogenic acid resource in honeysuckle leaf, directly affects the extraction amount of product, thereby reduces the economic value of chlorogenic acid resource development in honeysuckle leaf; (2) this method successively through

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  • Method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme
  • Method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme
  • Method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme

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Example Embodiment

[0020] Example 1

[0021] The specific steps of an enzyme-catalyzed activation method for extracting chlorogenic acid from honeysuckle leaves are as follows:

[0022] (1) Raw material pretreatment

[0023] Wash the honeysuckle leaves, dry them, place them in an oven, bake them in an oven at 100°C for 60 minutes, then take them out, pulverize them with a pulverizer, and sieve them through a 60 mesh molecular sieve to obtain honeysuckle leaves with a particle size of less than 60 meshes. Use a plastic bag Divide and spare.

[0024] (2) Enzymatic hydrolysis reaction

[0025] After the completion of step (1), the volume ratio of the mass of the solid waste of honeysuckle leaf after pretreatment in step (1): the mass of enzyme: water is 1:0.1:25. The enzyme is ligninase: cellulase in a mass ratio of 1:2. Add the compound enzyme and water to the raw materials pretreated in step (1). After stirring, adjust the pH of the system to 4 with sulfuric acid solution, and then place the pH-adjusted...

Example Embodiment

[0030] Example 2

[0031] An enzyme-catalyzed method for extracting chlorogenic acid from honeysuckle leaves is the same as that in Example 1, wherein:

[0032] In step (1), the honeysuckle leaf mesh number is 160 meshes, the oven temperature is 110°C, and the drying time is 30 minutes.

[0033] In step (2), the volume ratio of the mass of honeysuckle leaf solid waste: the mass of enzyme: water is 1:0.05:5, and the mass ratio of the enzyme is ligninase:cellulase is 1:0.5. The pH value of the system is adjusted to 8 with hydrochloric acid solution, and the temperature of the water bath is 65°C. Enzymatic hydrolysis activates honeysuckle leaf base material for 2h.

[0034] In step (3), the mass ratio of the pretreated honeysuckle leaf solid waste: ethanol solution volume ratio is 1:8. The ultrasonic power is 40W, the ultrasonic temperature is 55°C, the ultrasonic time is 10min; the centrifugal speed of the filtrate is 4000r / min, and the centrifugal time is 5min.

[0035] In step (4), t...

Example Embodiment

[0036] Example 3

[0037] An enzyme-catalyzed method for extracting chlorogenic acid from honeysuckle leaves is the same as that in Example 1, wherein:

[0038] In step (1), the honeysuckle leaf mesh number is 20 meshes, the oven temperature is 85°C, and the drying time is 90 minutes.

[0039] In step (2), the volume ratio of the mass of the pretreated honeysuckle leaf solid waste: the mass of enzyme: water is 1:0.3:30, and the mass ratio of the enzyme is ligninase: cellulase is 1: 3. Use hydrochloric acid solution to adjust the pH of the system to 3 and the temperature of the water bath to 20°C. Enzymatic hydrolysis activates honeysuckle leaf base material for 14h.

[0040] In step (3), the mass ratio of the honeysuckle leaf enzymatic hydrolysis activation base material: the volume ratio of the ethanol solution is 1:15. The ultrasonic power is 200W, the ultrasonic temperature is 25°C, the ultrasonic time is 45min; the centrifugal speed of the filtrate is 2500r / min, and the centrif...

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Abstract

The invention discloses a method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme, belonging to the technical field of chlorogenic acid extraction. According to the method, the solid waste of Lonicera japonica leaves, used as raw material, is prepared into the target product by the simple processes of treatment on Lonicera japonica leaves, enzymatic hydrolysis, ultrasonic wave synergistic digestion and chlorogenic acid preparation. The raw material used in the method provided by the invention is cheap and easily available, and the method has the characteristics that the waste is fully used, the prepared chlorogenic acid product is high in extraction ratio and purity and complete in biological activity, energy consumption in production process is low, the production equipment is hardly corroded, the consumption of organic solvent is low, environment protection is facilitated, and so on. The method provided by the invention can be widely used for extracting natural products, such as chlorogenic acid, isochlorogenic acid and Ginnol, from the solid waste of Lonicera japonica leaves, and effectively realizes the resource utilization of the Lonicera japonica leaves.

Description

technical field [0001] The invention belongs to the technical field of chlorogenic acid extraction, and in particular relates to a method for extracting chlorogenic acid by enzymatically activating honeysuckle leaves. Background technique [0002] Chlorogenic acid is an ester formed by caffeic acid and quinic acid, which belongs to phenylpropanoid compounds and is mainly found in plants such as honeysuckle, Eucommia, cocoa and coffee. As an important raw material for drug synthesis, chlorogenic acid has a significant effect on the prevention and treatment of cardiovascular system diseases and diabetes. It is also widely used in food preservation, anti-aging in cosmetics, and obesity prevention. It has broad applications in the fields of medicine and population health. market application prospects. However, chlorogenic acid itself is unstable. During the extraction process from plants, conditions such as high temperature, strong light, and long-term heating can accelerate th...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48
Inventor 王星敏
Owner 王星敏
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