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Method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme

A honeysuckle leaf, catalytic activation technology, applied in organic chemistry, separation/purification of carboxylic acid esters, etc., can solve the problem of chlorogenic acid extraction and purification process instability, affecting the economic development value of chlorogenic acid products, and increasing production energy consumption. and cost issues, to achieve the effect of ensuring extraction energy efficiency and product quality, reducing environmental treatment costs, and low production costs

Inactive Publication Date: 2013-05-08
王星敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main disadvantages of this method are: (1) the method adopts directly soaking honeysuckle leaves and eluting the soaking liquid to obtain chlorogenic acid, because the solubility of chlorogenic acid in water at 25°C is about 4%, and the chlorogenic acid in honeysuckle leaves at normal temperature The amount of acid soluble in soaking liquid is few, causes the waste of chlorogenic acid resource in honeysuckle leaf, directly affects the extraction amount of product, thereby reduces the economic value of chlorogenic acid resource development in honeysuckle leaf; (2) this method successively through three times of soaking, Purified water and ethanol are eluted twice, and the production steps are 7 steps in total. The extraction purity of the obtained chlorogenic acid is between 35% and 95%. The instability of acid extraction and purification process affects the quality of chlorogenic acid products and directly affects the economic development value of chlorogenic acid products; (3) in the production process, it only takes 10-14 hours to stand still in various steps, and the elution method used The additive ethanol is food grade, which further increases the production energy consumption and cost

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  • Method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme
  • Method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme
  • Method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme

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Effect test

Embodiment 1

[0021] The specific steps of a method for extracting chlorogenic acid by enzymatically activating honeysuckle leaves are as follows:

[0022] (1) Raw material pretreatment

[0023] Wash the honeysuckle leaves, dry them, place them in an oven, bake them in an oven at 100°C for 60 minutes, take them out, crush them with a pulverizer, and pass through a 60-mesh molecular sieve to obtain honeysuckle leaves with a particle size of less than 60 mesh. Subpackage, spare.

[0024] (2) Enzymolysis reaction

[0025] After the step (1) is completed, the mass of the honeysuckle leaf solid waste pretreated in the step (1): the mass of the enzyme: the volume ratio of water is 1:0.1:25. The enzyme is ligninase: cellulase with a mass ratio of 1:2. In the raw material pretreated in step (1), add compound enzyme and water, stir evenly, then use sulfuric acid solution to adjust the pH value of the system to 4, and then place the mixed solution after adjusting the pH value in a shaker, After p...

Embodiment 2

[0031] A method for extracting chlorogenic acid by enzymatically activating honeysuckle leaves, same as in Example 1, wherein:

[0032] In step (1), the mesh number of honeysuckle leaves selected is 160 mesh, the oven temperature is 110° C., and the drying time is 30 minutes.

[0033] In step (2), the mass of honeysuckle leaf solid waste: the mass of enzyme: the volume ratio of water is 1:0.05:5, and the enzyme is ligninase: the mass ratio of cellulase is 1:0.5. A hydrochloric acid solution was used to adjust the pH of the system to 8, and the temperature of the water bath was 65°C. Enzymolysis activated honeysuckle leaf base material for 2 hours.

[0034] In step (3), the mass of the pretreated honeysuckle leaf solid waste: the volume ratio of the ethanol solution is 1:8. The ultrasonic power is 40W, the ultrasonic temperature is 55°C, and the ultrasonic time is 10min; the filtrate is centrifuged at 4000r / min, and the centrifugation time is 5min.

[0035] In the (4) step, ...

Embodiment 3

[0037] A method for extracting chlorogenic acid by enzymatically activating honeysuckle leaves, same as in Example 1, wherein:

[0038] In the (1) step, the mesh number of honeysuckle leaves is 20 mesh, the oven temperature is 85° C., and the drying time is 90 minutes.

[0039] In the (2) step, the quality of the honeysuckle leaf solid waste after pretreatment: the quality of enzyme: the volume ratio of water is 1: 0.3: 30, and described enzyme is ligninase: cellulase mass ratio is 1: 3. A hydrochloric acid solution was used to adjust the pH of the system to 3, and the temperature of the water bath was 20°C. Enzymolysis activated honeysuckle leaf base material for 14 hours.

[0040] In step (3), the mass of honeysuckle leaf enzymolysis activation base material: the volume ratio of ethanol solution is 1:15. The ultrasonic power is 200W, the ultrasonic temperature is 25°C, and the ultrasonic time is 45min; the filtrate is centrifuged at 2500r / min, and the centrifugation time ...

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Abstract

The invention discloses a method for extracting chlorogenic acid from Lonicera japonica leaves activated under catalysis of enzyme, belonging to the technical field of chlorogenic acid extraction. According to the method, the solid waste of Lonicera japonica leaves, used as raw material, is prepared into the target product by the simple processes of treatment on Lonicera japonica leaves, enzymatic hydrolysis, ultrasonic wave synergistic digestion and chlorogenic acid preparation. The raw material used in the method provided by the invention is cheap and easily available, and the method has the characteristics that the waste is fully used, the prepared chlorogenic acid product is high in extraction ratio and purity and complete in biological activity, energy consumption in production process is low, the production equipment is hardly corroded, the consumption of organic solvent is low, environment protection is facilitated, and so on. The method provided by the invention can be widely used for extracting natural products, such as chlorogenic acid, isochlorogenic acid and Ginnol, from the solid waste of Lonicera japonica leaves, and effectively realizes the resource utilization of the Lonicera japonica leaves.

Description

technical field [0001] The invention belongs to the technical field of chlorogenic acid extraction, and in particular relates to a method for extracting chlorogenic acid by enzymatically activating honeysuckle leaves. Background technique [0002] Chlorogenic acid is an ester formed by caffeic acid and quinic acid, which belongs to phenylpropanoid compounds and is mainly found in plants such as honeysuckle, Eucommia, cocoa and coffee. As an important raw material for drug synthesis, chlorogenic acid has a significant effect on the prevention and treatment of cardiovascular system diseases and diabetes. It is also widely used in food preservation, anti-aging in cosmetics, and obesity prevention. It has broad applications in the fields of medicine and population health. market application prospects. However, chlorogenic acid itself is unstable. During the extraction process from plants, conditions such as high temperature, strong light, and long-term heating can accelerate th...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48
Inventor 王星敏
Owner 王星敏
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