Method for preparing euphausia superba oil, microcapsule of euphausia superba oil and low-fluorine euphausia superba peptide by using aqueous enzymatic method

A technology of Antarctic krill oil and Antarctic krill, applied in the direction of microcapsule preparation, microsphere preparation, fat oil/fat production, etc., can solve the problems of large amount of organic solvent usage, separation of organic solvent, and difficulty in recovery, etc., to overcome extraction Incomplete, no solvent residue, avoiding the effect of solvent residue

Active Publication Date: 2015-03-04
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When leaching with a single organic solvent, the extraction efficiency is low. When extracting Antarctic krill oil abroad, a strong polar organic solvent and a weak polar organic solvent are used for step-by-step extraction. This method is called "two footwork"
Although the two-step method can improve the extraction efficiency, there are also problems such as cumbersome operation process, large amount of organic solvent used, separation and recovery of organic solvent, etc.
In addition, the desolvation of shrimp oil extracted by organic solvent extraction generally requires high temperature, which will destroy the structure of the heat-sensitive functional active ingredient astaxanthin in Antarctic shrimp oil, and affect the quality of shrimp oil
In addition to organic solvent extraction, there is also a greener extraction method, supercritical CO 2 The extraction method can extract Antarctic krill oil without using organic solvents, but the shrimp oil extracted by this method is mainly neutral oils such as triglycerides, and the content of polar oils - phospholipids is very low, and phospholipids are just The characteristic functional ingredients of Antarctic krill oil, so purely use supercritical CO 2 Extraction Difficult to Achieve Adequate Extraction of Antarctic Krill Oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Antarctic krill (whole shrimp) was heat-treated in a water bath at 100°C for 30 minutes, and homogenized.

[0014] (2) Add 1 times of water by weight and stir evenly. Use 1mol / L sodium hydroxide solution to adjust the pH value of the homogenate to 8, add food-grade alkaline protease (EC3.4.21.62), the enzyme dosage is 250U / g krill raw material, and enzymolyze at 40°C for 0.5h . Heat the enzymolysis solution to boiling and keep boiling for 10 minutes to inactivate protease. The enzymolysis solution was cooled to 10°C and centrifuged at 8000×g for 10 minutes. The centrifuged enzymatic hydrolyzate can be divided into 4 layers, from top to bottom are free oil layer + emulsion layer I, water layer, emulsion layer II, and residue layer. The oil recovery rate of the obtained free oil layer + emulsion layer I and emulsion layer II was 60.65±2.07%, and the phospholipid content accounted for 51.62±1.08% of lipids.

[0015] (3) Collect the free oil layer + emulsion layer I...

Embodiment 2

[0018] (1) Antarctic krill (whole shrimp) was heat-treated in a water bath at 100°C for 30 minutes, and homogenized.

[0019] (2) Add 4 times of water by weight and stir evenly. Use 1mol / L sodium hydroxide solution to adjust the pH value of the homogenate to 10, add food-grade alkaline protease (EC3.4.21.62), the enzyme dosage is 1000U / g krill raw material, and enzymolyze at 60°C for 2.5h . Heat the enzymolysis solution to boiling and keep boiling for 30 minutes to inactivate protease. The enzymolysis solution was cooled to 40°C and centrifuged at 13500×g for 30 minutes. The centrifuged enzymatic hydrolyzate can be divided into 4 layers, from top to bottom are free oil layer + emulsion layer I, water layer, emulsion layer II, and residue layer. The oil recovery rate of the obtained free oil layer+emulsion layer I and emulsion layer II was 61.51+0.58%, and the phospholipid content accounted for 61.11±0.89% of lipids.

[0020] (3) Collect the free oil layer + emulsion layer ...

Embodiment 3

[0023] (1) Antarctic krill (whole shrimp) was heat-treated in a water bath at 100°C for 30 minutes, and homogenized.

[0024](2) Add 2 times of water by weight and stir evenly. Use 1mol / L sodium hydroxide solution to adjust the pH value of the homogenate to 9, add food-grade alkaline protease (EC3.4.21.62), the enzyme dosage is 750U / g krill raw material, and enzymolyze at 50°C for 1.5h . Heat the enzymolysis solution to boiling and keep boiling for 20 minutes to inactivate protease. The enzymolysis solution was cooled to 20°C and centrifuged at 13500×g for 15 minutes. The centrifuged enzymatic hydrolyzate can be divided into 4 layers, from top to bottom are free oil layer + emulsion layer I, water layer, emulsion layer II, and residue layer. The oil recovery rate of the obtained free oil layer + emulsion layer I and emulsion layer II was 64.77±0.12%, and the phospholipid content accounted for 65.58±1.02% of lipids.

[0025] (3) Collect the free oil layer + emulsion layer I...

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Abstract

The invention discloses a method for preparing euphausia superba oil, a microcapsule of the euphausia superba oil and low-fluorine euphausia superba peptide by using an aqueous enzymatic method. The method comprises the following steps: thermally treating and homogenizing a euphausia superba raw material, subsequently performing enzymolysis and centrifuging, wherein the centrifuged enzymatic hydrolysate is divided into four layers, respectively including a free oil layer + an emulsion layer I, a water layer, an emulsion layer II and a residue layer from top to bottom; collecting the free oil layer + emulsion layer I and the emulsion layer II, taking the free oil layer + emulsion layer I and the emulsion layer II as core materials, heating and mixing Arabic gum and gelatin to obtain a mixed solution which is taken as a wall material, emulsifying, homogenizing and then performing spray-drying to obtain a euphausia superba microcapsule; filtering the water layer by a ceramic membrane, carrying out vacuum concentration on the filtrate and then spray-drying to obtain low-fluorine euphausia superba peptide powder. The method disclosed by the invention is green, friendly to environment and free of solvent residues, prevents unsafe factors such as solvent residues caused by a solvent method and overcomes the problem of incomplete extraction of lipid compositions in a supercritical fluid extraction method; moreover, the method disclosed by the invention is used for respectively preparing the euphausia superba oil, the euphausia superba microcapsule and the low-fluorine euphausia superba peptide, so that the variety of euphausia superba oil products is enriched.

Description

technical field [0001] The invention relates to the deep processing technology of Antarctic krill, more specifically, relates to a method for preparing Antarctic krill oil, its microcapsules and low-fluorine Antarctic krill peptide by aqueous enzymatic method. Background technique [0002] Antarctic krill (Euphausia superba) is one of the largest and most successful single species of biological resources on the earth. According to the data released by the Food and Agriculture Organization of the United Nations, the biomass of Antarctic krill is about 125-725 million tons, and the annual catchable amount is more than 13 million tons. It is an important potential fishery resource. In recent years, with the gradual depletion of world-wide traditional fishery resources and the proposal of a 200 nautical mile exclusive economic zone, the huge Antarctic krill resources in Antarctic waters have attracted the attention of some pelagic fishery developed countries. Since 2009, my cou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/02B01J13/02C12P21/06
CPCB01J13/043B01J13/046C11B1/00C11B1/02C11B1/025C12P21/06Y02P20/54
Inventor 周大勇徐文思宋泽宇潘锦锋朱蓓薇詹佳新王风雅何超琪
Owner DALIAN POLYTECHNIC UNIVERSITY
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