The invention relates to a method for preparing 2-C-methyl-D-ribotide-1,4-
lactone, which comprises the following steps: adding a
raw material D-glucose into dibenzyl amine,
acetic acid and
ethanol at
room temperature, dissolving by stirring, heating, and refluxing to generate 1-
desoxy-1-(N,N-amino)-D-
fructose; after finishing the reaction, cooling to
room temperature to obtain a white
solid,
drying to obtain 1-
desoxy-1-(N,N-amino)-D-
fructose, dissolving the 1-
desoxy-1-(N,N-amino)-D-
fructose and CaO in
ethanol and water by stirring, and heating under
reflux; and after finishing the reaction, cooling, carrying out centrifugal
drying to remove the
solvent, adding water and
oxalic acid hydrate to precipitate abundant
calcium oxalate, filtering, washing, carrying out centrifugal
drying on the filtrate to obtain a black
viscous liquid, adding
acetone, cooling by standing, and recrystallizing with
acetone to obtain the 2-C-methyl-D-ribotide-1,4-
lactone. The method has the advantages of reasonable technique, low cost, short reaction time and high yield, and is simple to operate.