15 results about "Nucleoside diphosphate" patented technology

The current invention concerns methods for the synthesis of 6-azido-6-deoxy-2-N-acetyl-monosaccharide-nucleoside diphosphate, in particular 6-azido-6-deoxy-2-N-acetyl-D-galactosamine-nucleoside diphosphate or 6-azido-6-deoxy-2-N-acetyl-D-glucosamine-nucleoside diphosphate. The synthesis method according to the invention is characterized by being highly efficient and high yielding. Also part of the present invention are key intermediates of this process.

The current invention concerns methods for the synthesis of 6-azido-6-deoxy-2-N-acetyl-monosaccharide-nucleoside diphosphate, in particular 6-azido-6-deoxy-2-N-acetyl-D-galactosamine-nucleoside diphosphate or 6-azido-6-deoxy-2-N-acetyl-D-glucosamine-nucleoside diphosphate. The synthesis method according to the invention is characterized by being highly efficient and high yielding. Also part of the present invention are key intermediates of this process.

Disclosed are methods and compositions for glycosylation of a sugar acceptor, comprising the steps of: (i) converting an exogenous nucleoside triphosphate (NTP) to a nucleoside diphosphate (NDP); (ii) converting the NDP to NDP-sugar, comprising contacting at least one Leloir glycosyltransferase (GT) with the NDP and an exogenous sugar donor (SD) in a reaction medium; and (iii) converting a sugar acceptor (SA) to a glycosylated SA, comprising contacting the at least one GT with the NDP-sugar and the SA in the reaction medium.

The present disclosure provides enzymatic methods for the production of 3'-phosphatenucleoside-5'-triphosphate (NQP). The present disclosure further provides enzymatic methods for the production of nucleotide-5'-triphosphates. In some embodiments, a method of producing a nucleotide triphosphate with a phosphate group at the 3' position of the sugar moiety (NQP), the method comprising at least: contacting a nucleoside diphosphate (NDP) with a nucleoside diphosphate kinase, a 3' -O-kinase, and a phosphate donor under reaction conditions such that a NTP with a phosphate group on the 3' position of the sugar moiety (NQP) is produced.

The application relates to the technical field of biological medicine, and discloses a nucleoside diphosphate compound and a preparation method thereof. The compound has the structure shown in formula (I). The preparation method of the nucleoside compound comprises the following steps: contacting a compound shown in formula (I-2) with a compound shown in formula (I-1) to perform a contact reaction I. The aforementioned nucleoside compound provided by the application has excellent inhibitory activity on CD73, and in-vivo experiments show that the compound has good antitumor activity.

The present invention relates to a method for the synthesis of 6-azido-6-deoxy-2-N-acetyl-monosaccharide-nucleotide diphosphates, in particular 6-azido-6-deoxy-2-N-acetyl-D-galactosamine-nucleotide diphosphate or 6-azido-6-deoxy-2-N-acetyl-D-glucosamine-nucleotide diphosphate. The synthesis method according to the present invention is characterized by high efficiency and high yield. Another part of the present invention is a key intermediate of the method.

The present application discloses a method for purifying a fluorescein-labeled nucleoside triphosphate. In the present application, a liquid chromatography method is used to purify a crude product of a fluorescein-labeled nucleoside triphosphate, and a target fraction is collected; wherein, the mobile phase comprises a first component and a second component, the first component is a hexylamine acetate aqueous solution, and the second component is selected from acetonitrile or methanol. The method for purifying a fluorescein-labeled nucleoside triphosphate of the present invention can separate a nucleoside diphosphate, which is similar in structure to the nucleoside triphosphate and difficult to separate, from the target product in the crude product of the fluorescein-labeled nucleoside triphosphate, and obtain a fluorescein-labeled nucleoside triphosphate with a purity greater than 99%. The fluorescein-labeled nucleoside triphosphate purified by the method for purifying a fluorescein-labeled nucleoside triphosphate of the present invention has good fluorescence intensity and high sensitivity, meeting the requirements of fluorescence in situ hybridization experiments.

The present invention addresses the problem of providing a novel method for synthesizing a deoxynucleoside triphosphate or nucleoside triphosphate.　This method for synthesizing a deoxynucleoside triphosphate (dNTP) from a deoxyribonucleoside or synthesizing a ribonucleoside triphosphate (NTP) from a ribonucleoside is characterized by: adding, to a reaction vessel, (i) a deoxyribonucleoside or ribonucleoside as a starting material, (ii) a kinase capable of producing a deoxyribonucleoside monophosphate from the deoxyribonucleoside or a ribonucleoside monophosphate from the ribonucleoside, a kinase capable of producing a deoxyribonucleoside diphosphate from the deoxyribonucleoside monophosphate or a ribonucleoside diphosphate from the ribonucleoside monophosphate, and a pyruvate kinase as enzymes, and (iii) a nucleoside triphosphate or deoxynucleoside triphosphate and a phosphoenolpyruvic acid (PEP) as phosphoric acid donors, thereby adjusting a reaction solution; and reacting the same in one pot.

To provide a method for producing rebaudioside D and rebaudioside M by reaction of glycosyltransferase, and to provide a composition for producing rebaudioside D and rebaudioside M containing glycosyltransferase.SOLUTION: Provided is a method for preparing rebaudioside D, the method including preparing rebaudioside D by reacting glucose-linked nucleotide diphosphate with rebaudioside A in the presence of glycosyltransferase B (UGT-B), wherein the glycosyltransferase B is at least one protein selected from the group consisting of proteins consisting of an amino acid sequence represented by a specific sequence.SELECTED DRAWING: Figure 1

The present invention provides engineered adenylyl-sulfate kinase polypeptides capable of adding a phosphate group to the 3' position of the sugar of a natural or modified nucleoside diphosphate, as well as compositions and methods of utilizing these engineered polypeptides.

The present invention belongs to the field of biomedicine, and particularly relates to the application of overexpressing Nudt12 in the preparation of drugs for treating acute myocardial infarction. The present invention discloses the application of nucleoside diphosphate-linked moiety X motif 12 (Nudt12) in the preparation of drugs for treating acute myocardial infarction. By establishing a model of apoptosis induced by ischemia and hypoxia in neonatal mouse cardiomyocytes and a model of acute myocardial infarction induced by ligation of the left anterior descending coronary artery in mice, the present invention finds that after overexpression of Nudt12, it can significantly reduce the oxidative stress and apoptosis level of cardiomyocytes under ischemia and hypoxia conditions, increase the synthesis of ATP, enhance the anti-apoptosis ability of cardiomyocytes, significantly reduce myocardial fibrosis and the area of myocardial infarction after myocardial infarction, maintain the contraction of cardiomyocytes, and improve the cardiac function of mice with myocardial infarction. The present invention provides a new target and strategy for the clinical diagnosis and treatment of heart failure caused by acute myocardial infarction.

To provide an improved method for producing a sugar nucleoside diphosphate in the field of a functionalized nucleoside sugar.SOLUTION: A method for the chemical conversion of GalNAc or GlcNAc to its respective 6-azido derivative via a cyclic sulfate, followed by conversion to the respective UDP derivative via an anomeric phosphorylation step and a UMP coupling step, wherein a 6-azido-6-deoxy monosaccharide compound having the structure (I) is prepared according to the following scheme: SELECTED DRAWING: None

This invention relates to a method for synthesizing 6-azido-6-deoxy-2-N-acetyl-monosaccharide-nucleoside diphosphate, particularly 6-azido-6-deoxy-2-N-acetyl-D-galactosamine-nucleoside diphosphate or 6-azido-6-deoxy-2-N-acetyl-D-glucosamine-nucleoside diphosphate. The synthetic method according to the invention is characterized by high efficiency and high yield. Another part of the invention concerns the key intermediates of this method.

Disclosed is a method for evaluating the oxidative activity of a dehydrogenase, which comprises a step in which the dehydrogenase and the DT-diaphorase are subjected to a catalytic oxidation-reduction reaction in an aqueous solution containing one molecule of the dehydrogenase, a substrate for the dehydrogenase, a nucleoside diphosphate derivative, the DT-diaphorase, and a fluorescent probe, and fluorescence after the reaction is measured.

The invention provides a catalytic system. The catalytic system is used for catalyzing nucleoside monophosphate into nucleoside diphosphate; the catalytic system comprises nucleotide kinase, acetate kinase, acetyl phosphate or salts thereof, a phosphoric acid donor, inorganic pyrophosphatase and a solvent. The method has the beneficial effects that a catalytic system of nucleotide kinase, acetate kinase and inorganic pyrophosphatase is simple and efficient, nucleoside monophosphate can be converted into nucleoside diphosphate in one step, and the method is particularly suitable for converting 3, 7-dimethyl guanosine monophosphate into 3, 7-dimethyl guanosine diphosphate. The catalytic system provided by the invention is high in conversion efficiency and can be used for large-scale production.