Diphosphoric uridine glucosyl transferase gene and encoded protein thereof

A diphosphourea-based, glucose-based technology, applied in the field of protein, to achieve the effects of variety improvement, resistance improvement, and content reduction

Inactive Publication Date: 2008-12-03
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Diphosphoric uridine glucosyl transferase gene and encoded protein thereof
  • Diphosphoric uridine glucosyl transferase gene and encoded protein thereof
  • Diphosphoric uridine glucosyl transferase gene and encoded protein thereof

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Embodiment Construction

[0015] 1. Using chip information to screen candidate genes in the DON-induced panicle cDNA library

[0016] Wheat variety Wangshuibai (commonly known public materials, analysis of combining ability of DON content in common wheat grains such as Pei Ziyou, Jia Gaofeng, etc. ACTA AGRONOMICA SINICA 2007, 33(5): 731-737) was inoculated with deoxynivalenol ( DON) (Sigma) in water (100 ppm), inoculated with water as a control. Ear samples were taken 12 and 24 hours after inoculation, and total RNA was extracted with TRIZOL (invitogen) according to the reagent instructions. The two RNAs induced by DON were mixed in equal amounts as the experimental group, and the two RNAs inoculated in water were mixed in equal amounts as the control group. The RNA of the experimental group and the control group was hybridized to the Affymetrix wheat gene expression profile chip (part number900559) (Affymetrix). The experiment was completed at the "Shanghai National Biochip Engineering Center". http...

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Abstract

The invention discloses a nucleoside diphosphate glucosyltransferase gene (Ta-UGT) and coded protein thereof, and belongs to the gene engineering field. The cDNA sequence of the nucleoside diphosphate glucosyltransferase gene (Ta-UGT) is SEQ ID NO.1, the sequence of coded amino acid thereof is SEQ ID NO.2, and the gene is from wheat scab (triticum asetivum L.), which is reported for the first time in the wheat and induced by deoxynivalenol (DON) to strengthen expression. An experiment shows that the over expression of the gene can improve the resistibility of plants to DON. The Ta-UGT is introduced into the wheat variety easily infected by gibberellic disease, so as to expect to reduce the content of the DON in the wheat grain.

Description

1. Technical field: [0001] The invention discloses a diphosphourinoglucosyltransferase gene (Ta-UGT) from wheat (Triticum asetivum L.) Wangshuibai and its coded protein, which belongs to the field of genetic engineering. Mildew wheat varieties, may reduce DON content in wheat kernels. 2. Background technology: [0002] Wheat head blight (FHB) is a fungal disease that has become a worldwide devastating disease. It can cause diseases to crops such as wheat, barley and other small grains and corn, directly causing crop yield reduction. On the other hand, harvested grains were degraded by contamination with deoxynivalenol (DON) and some other trichothecenetoxins. These toxins are very harmful to humans and animals. Among this class of toxins, DON is the most prevalent class of toxins present in food in many countries, including China. In order to protect the safety of consumers, many countries have imposed certain restrictions on the content of DON in wheat flour. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N9/10
Inventor 刘大钧马璐琳王秀娥亓增军陈佩度曹爱忠邢莉萍
Owner NANJING AGRICULTURAL UNIVERSITY
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