Diphosphoric uridine glucosyl transferase gene and encoded protein thereof

Abstract

The invention discloses a nucleoside diphosphate glucosyltransferase gene (Ta-UGT) and coded protein thereof, and belongs to the gene engineering field. The cDNA sequence of the nucleoside diphosphate glucosyltransferase gene (Ta-UGT) is SEQ ID NO.1, the sequence of coded amino acid thereof is SEQ ID NO.2, and the gene is from wheat scab (triticum asetivum L.), which is reported for the first time in the wheat and induced by deoxynivalenol (DON) to strengthen expression. An experiment shows that the over expression of the gene can improve the resistibility of plants to DON. The Ta-UGT is introduced into the wheat variety easily infected by gibberellic disease, so as to expect to reduce the content of the DON in the wheat grain.

Description

1. Technical field: [0001] The invention discloses a diphosphourinoglucosyltransferase gene (Ta-UGT) from wheat (Triticum asetivum L.) Wangshuibai and its coded protein, which belongs to the field of genetic engineering. Mildew wheat varieties, may reduce DON content in wheat kernels. 2. Background technology: [0002] Wheat head blight (FHB) is a fungal disease that has become a worldwide devastating disease. It can cause diseases to crops such as wheat, barley and other small grains and corn, directly causing crop yield reduction. On the other hand, harvested grains were degraded by contamination with deoxynivalenol (DON) and some other trichothecenetoxins. These toxins are very harmful to humans and animals. Among this class of toxins, DON is the most prevalent class of toxins present in food in many countries, including China. In order to protect the safety of consumers, many countries have imposed certain restrictions on the content of DON in wheat flour. [0003] ...

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Problems solved by technology

However, no reports of such genes have been found in wheat

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