Monoclonal antibody and ELISA method and kit for detecting carbadox and cydox metabolites

A monoclonal antibody and metabolite technology, applied in the field of veterinary drug residue analysis and immunology, to achieve high accuracy, save time and cost, and minimize health hazards
CN104558186BInactive Publication Date: 2017-07-04HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2017-07-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a specificity monoclonal antibody capable of distinguishing various carbadox and cyadox metabolism products and an enzyme linked immunosorbent assay method and kit for detecting the carbadox and cyadox metabolism products. According to the invention, the monoclonal antibody is secreted by a hybridoma cell strain DCBX5B1 of which the preservation number is CCTCC NO: C201495. Compared with the prior art, the monoclonal antibody disclosed by the invention can distinguish DCBX, bisdesoxy cyadox and desoxy cyadox (N4) at the same time, and has higher sensitivity and specificity. The ELISA method and the kit, disclosed by the invention, can detect the residue of the carbadox and cyadox metabolism products in meat food at the same time, and have the advantages of high detection efficiency, high sensitivity, high precision, high accuracy and the like.
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Description

technical field

[0001] The invention belongs to the technical field of veterinary drug residue analysis and immunology, and relates to a monoclonal antibody capable of recognizing carbadox and cycadol metabolites and an enzyme-linked immunosorbent method (ELISA) for detecting carbadox and cycadol metabolites. ) and kits. Background technique

[0002] Dedioxycarboxylate and dedioxycydol are quinoxaline drugs, which are the products of the deoxygenation and metabolism of the prototype drugs carbadox and cyadox in animals. structure, carbadox and quincedo have a broad antibacterial spectrum, and their anti-gram-negative bacteria are stronger than positive bacteria. It has been widely used in veterinary clinic and breeding industry. People abuse drugs in pursuit of economic benefits, resulting in a large amount of drug residues in animal food. Quinoxaline drugs can cause toxic and side effects such as tertiary effects, photosensitivity, and adrenal cortex damage to animals. S...

Claims

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