A kind of deoxynojirimycin extraction method

Abstract

The invention discloses a method for extracting a deoxynojirimycin monomer. The method comprises the following steps of: soaking a mulberry raw material in acidic aqueous solution, and extracting for 1 to 3 times; adsorbing extracting solution on a cation exchange resin, washing, and eluting by using ammonia water; performing reverse osmosis pre-concentration on eluent, recovering ammonia, dissolving an intermediate in a hydrophilic solvent, performing catalytic conversion, allowing the solution to pass through a gel chromatographic column, and eluting the hydrophilic solvent; recovering the solvent, dissolving in a mixed solvent, allowing the mixed solvent to pass through the chromatographic column, and eluting the mixed solvent; and recovering the mixed solution to obtain pure deoxynojirimycin products. The method is low in energy consumption and environment-friendly, and the pure deoxynojirimycin products with various purity grades of 10 percent, 50 percent, 99 percent and the like.

Description

technical field [0001] The invention relates to biological extraction technology, in particular to a method for extracting deoxynojirimycin monomer. Background technique [0002] 1-deoxynojirimycin (1-deoxynojirimycin, DNJ) is a polar N-containing compound, its chemical name is 3, 4, 5-trihydroxy-2-hydroxymethyltetrahydropyridine, and its molecular formula is C 6 h 13 NO 4 , with a relative molecular mass of 163. DNJ has a strong inhibitory effect on α-glucosidase, and is an effective ingredient for lowering blood sugar and treating diabetes [1～4] , and antiviral [5] , anti-tumor metastasis [6] and other pharmacological effects. In recent years, scholars at home and abroad have conducted a lot of research and exploration on the source of DNJ, the synthesis mechanism in vivo and chemical synthesis methods, hoping to find a stable, efficient and economical way to obtain DNJ [7] . [0003] Li Yuliang [8] reported a method for extracting deoxynojirimycin from mulberry l...

AI Technical Summary

Problems solved by technology

This method requires that the "total alkaloids" of the raw material contain more than 40% of deoxynojirimycin, and the step-by-step eluents are alcohol solutions of different concentrations, and the recrystallization is a mixed solution of methanol and acetone; a large amount of detection work is required during the step-by-step elution And the collection of pure products, the operation is more troublesome, and the mixed solvent of recrystallization is difficult to handle

[0013] Chinese patent technology (200810236144.1) reported a compound extraction process for the co-production of mulberry leaf flavonoids, polysaccharides and alkaloids, but did not separate and obtain pure deoxynojirimycin

This process requires the use of ultrafiltration technology, ethanol distributed elution, and the collection of the target eluent requires continuous detection of the effluent components, etc. The operation is complicated; the energy consumption is high

[0020] So far, from the existing literature and patented technology, the extraction of pure deoxynojirimycin or the use of too much organic solvent, especially the use of chloroform, lead to low safety; or require multiple concentrations and high energy consumption ; or need to use too many steps, or need to detect and collect target components step by step, because deoxynojirimycin has no ultraviolet absorption, detection is more troublesome, complex operation, difficult to control industrial production; or can not get pure products, etc.