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Preparation method of mulberry leaf extract containing 1-deoxynojirimycin

A technology of deoxynojirimycin and mulberry leaf extract, which is applied in the direction of organic chemistry, can solve the problems of high cost, membrane pores will be blocked and polluted, use, etc., and achieve low cost, easy industrialization, and reduce environmental pollution Effect

Active Publication Date: 2016-02-03
内蒙古昶辉生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of membrane separation technology, the membrane pores will inevitably be blocked and polluted, which requires regular cleaning and inspection, and the cost is high
[0009] It can be found that there are many problems in the existing methods for preparing the mulberry leaf extract containing 1-deoxynojirimycin, either a large amount of toxic solvents are used, or the cost is high, or regular treatment is required, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Get 5kg of mulberry leaves, cook in 3% salt water for 1 hour, and cool naturally; move to the extraction tank, add water twice the weight of the raw material, and add the compound enzyme of pectinase and hemicellulase by 2% of the water amount ( 2:1), stirring and extracting at room temperature for 2 hours, filtering to obtain the extract; concentrating the extract into an extract, dissolving the sample in 50% ethanol, separating it through a polyamide column, and using a mobile phase of 3% phosphoric acid solution-ethanol (1:4) elution, collect the eluent; separate the eluent through simulated moving bed chromatography, wherein the adsorbent filled in the simulated moving bed chromatography is silica gel, and the eluent is 0.1% triethylamine aqueous solution; the adsorption zone The flow rate is 2BV / h; the flow rate in the elution zone is 2BV / h; the switching time is 600s; the temperature is controlled at 25°C; the pressure is controlled at 0.3MPa; the mulberry leaf sep...

Embodiment 2

[0031] Take 5kg of mulberry leaves, boil them in 1% salt water for 2 hours, and cool them naturally; move them to the extraction tank, add water 1 times the weight of the raw materials, and add pectinase and hemicellulase compound enzymes according to 3% of the water amount ( 2:1), stirring and extracting at room temperature for 1 hour, filtering to obtain the extract; concentrating the extract into an extract, dissolving the sample in 50% ethanol, separating it through a polyamide column, and using a mobile phase of 3% phosphoric acid solution-ethanol (1:4) elution, collect the eluent; separate the eluent through simulated moving bed chromatography, wherein the adsorbent filled in the simulated moving bed chromatography is silica gel, and the eluent is 0.1% triethylamine aqueous solution; the adsorption zone The flow rate is 2BV / h; the flow rate in the elution zone is 3BV / h; the switching time is 700s; the temperature is controlled at 20°C; the pressure is controlled at 0.4MPa...

Embodiment 3

[0033] Get 5kg of mulberry leaves, cook in 5% salt water for 0.5 hours, and cool naturally; move to the extraction tank, add water 3 times the weight of the raw material, and add pectinase and hemicellulase compound enzyme by 1% of the water amount ( 2:1), stirring and extracting at room temperature for 3 hours, filtering to obtain the extract; concentrating the extract into an extract, dissolving the sample in 50% ethanol, separating it through a polyamide column, and using a mobile phase of 3% phosphoric acid solution-ethanol (1:4) elution, collect the eluate; separate the eluate through simulated moving bed chromatography, wherein the adsorbent filled in the simulated moving bed chromatography is silica gel, and the eluent is 0.1% triethylamine aqueous solution; the adsorption zone The flow rate is 1BV / h; the flow rate in the elution zone is 2BV / h; the switching time is 700s; the temperature is controlled at 40°C; the pressure is controlled at 0.5MPa; the mulberry leaf separ...

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PUM

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Abstract

The invention provides a preparation method of mulberry leaf extract containing 1-deoxynojirimycin. The method mainly including the steps of saline solution water-proof cooking and softening, enzymolysis, polyamide column purification and simulated moving bed refining is determined by sufficiently considering the raw material characteristics of mulberry leaves and the dissolution characteristics of the target component 1-deoxynojirimycin and conducting system screening and optimizing. The separation effect is extremely good, and the concentration of 1-deoxynojirimycin in the finally obtained mulberry leaf extract is larger than 95 wt%. All materials used for the whole method are conventional materials, cost is low, yield is high, solvent can be recycled, environment pollution can be reduced, and industrialization can be easily achieved.

Description

technical field [0001] The invention relates to a preparation method of mulberry leaf extract, which belongs to the field of natural plant extracts. Background technique [0002] Mulberry Leaf, (Mulberry Leaf), scientific name: Folium Mori, is the dry leaf of the Moraceae plant mulberry, also known as home mulberry, Vitex mulberry, mulberry tree, yellow mulberry leaf, etc., cultivated or wild, it is widely planted in the north and south of my country, and the output of mulberry leaves Rich. Modern scientific research shows that mulberry leaves contain flavonoids, polysaccharides, vitamins, minerals, amino acids and other chemical components, which have physiological effects such as lowering blood sugar, lowering blood pressure, anti-aging, antibacterial, and anti-virus, especially mulberry leaves. Studies have shown that the polyhydroxyalkaloids contained in the drug have strong hypoglycemic activity, which has become a research hotspot in recent years. [0003] The represe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D211/46
CPCC07D211/46
Inventor 马亚琼赵永强高瑞梁玉强贾洪涛
Owner 内蒙古昶辉生物科技股份有限公司
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