42 results about "Alpha-Glucosidases" patented technology

The invention relates to the preparation of tea and aims to provide a preparation method of ultramicro mulberry leaf tea powder. For obtaining ultramicro mulberry leaf powder and ultramicro tea powder, the method comprises the following steps of: selecting raw materials, killing out, kneading, drying, primarily crushing and ultromicro crushing; sufficiently mixing ultramicro mulberry leaf powder with a nojirimycin crude product with a V type mixer, and sufficiently mixing the mixture with ultramicro tea powder to obtain the ultromicro mulberry leaf tea powder, wherein the weight content of nojirimycin in the nojirimycin crude product is 10-12 percent; and finally packing. For the ultromicro mulberry leaf tea prepared by the invention, ultromicro crushed to obtain the grain size less than 10 mum, the powder can be sufficiently suspended in hot water and can be completely taken, thereby the health care and nutritious value of the mulberry leaf are fully exerted. The nojirimycin crude product is added in the ultramicro mulberry leaf tea powder so that the activity of alpha-glucosidase can be more effectively restrained, glucose entering the blood is reduced, and the blood sugar is reduced. The invention can be used for health care or auxiliarily treating diabetes.

The present invention relates to a pentacyclic triterpanoid derivative of multiple-oxide substitution of the A ring and the medicine salt or solvate of the derivative, and the present invention also relates to the preparation method, the drug combination, and medical use of the derivative. The compound of the present invention has the functions of inhibiting the activity of six human tumor cell strains in vitro, such as human prostate cancer cell (PC-3), nasopharyngeal carcinoma cells (CNE), oral squamous carcinoma cell(KB), human lung cancer cell (A549), human hepatoma cell (BEL-7404), and human cervix cancer cell (Hela), and the function of the invention is at the same magnitude of the positive control of cisplatin, thereby the compound can be used as expected antitumor drug. The compound of the present invention also inhibits the alpha glucosidase strongly, and the inhibiting effect is greater than the positive control of acarbose, thereby the compound can be used as expected medicine for preventing and treating diabetes and the treatment of the virus diseases.

The methods of the invention relate to the treatment of diabetes, including type 2 diabetes, and related disorders by administration of a PDE10A inhibitor. Such PDE10A inhibitors may be administered in conjunction with alpha-glucosidase inhibitors, insulin sensitizers, insulin secretagogues, hepatic glucose output lowering compounds, B-3 agonist, or insulin. Such PDE10A inhibitors may also be administered in conjunction with body weight reducing agents. Further methods of the invention relate to stimulating insulin release from pancreatic cells, for example, in response to an elevation in blood glucose concentration, by administration of a PDE10A inhibitor.

The invention relates to a method of using alpha-glucosidase for preparing isomaltooligosaccharide. The method includes alpha-glucosidase immobilization, continuous reaction and isomaltooligosaccharide preparation. The alpha-glucosidase is immobilized through alginate, carrageenan and chitosan to implement continuous reaction, converting rate of raw material is 75%, and production cost is reduced by 1 / 3. By the aid of the method, isomaltooligosaccharide 50 or total isomaltooligosaccharide content of the isomaltooligosaccharide 50 is 55%, and the total content of isomalt, panose and isomaltotriose is 35%; isomaltooligosaccharide 90 or total isomaltooligosaccharide content of the isomaltooligosaccharide 90 is 95%, and the the total content of isomalt, panose and isomaltotriose is 70%.

The invention provides a compound having an alpha-glucosidase inhibitory activity in lotus leaves. Nine compounds having the alpha-glucosidase inhibitory activity are screened from the lotus leaves through magnetic bead separation and liquid chromatogram-mass spectrometry analysis, and the obtained compounds are confirmed to have the alpha-glucosidase inhibitory activity through a verification of the alpha-glucosidase inhibitory activity. Compared with tradition methods, separated compounds are not needed to prepare during a primary screening, thereby substantially improving screening efficiency and shortening screening time. Due to changing a traditional activity-screening mode which separates firstly and screens secondly to a novel activity-screening mode which screens firstly and separates secondly, blindness of preparing and separating compounds is greatly reduced. The method can be popularized and applied to quick screening of compounds having the alpha-glucosidase inhibitory activity from natural medicines or herbal mixtures. The obtained compounds can be used for preparing anti-diabetic drugs.

The invention discloses a preparation method of sargassum oligosaccharide. The preparation method comprises the steps that fine polysaccharide obtained through degreasing and deproteinizing of sargassum is taken as a raw material, ultrasonic treatment is utilized, alginate lyase, mannase, xylanase and pectinase are sequentially added to perform enzymolysis on the polysaccharide, the polysaccharide which is not degraded fully is removed through an ethyl alcohol sedimentation method, supernate is centrifuged and screened by a molecular sieve to obtain retained matter, freeze drying is performed, and the sargassum oligosaccharide is prepared. The prepared sargassum oligosaccharide has the high inhibitory activity on alpha-glucosidase, the IC50 value is 4.82 mg / mL, the dose dependency is presented, and meanwhile the obvious promoting effect on glucose consumption of insulin-resistant HepG2 cells is achieved. According to the preparation method, the non-specific commercial enzymes are adopted, the process route is simple and reasonable, the method is suitable for industrialization, the preparation amount of the hypoglycemic active oligosaccharide is increased, and meanwhile the enzyme consumption and production cost are reduced; the method is an effective method for preparing the sargassum oligosaccharide and can be applied to hypoglycemic drugs, health care products and food.

The invention provides alpha-glucosidase. The amino acid sequence of the alpha-glucosidase is SEQ ID NO:1. The optimum action temperature of the alpha-glucosidase is 60 DEG C; over 80% of enzyme activity can be retained in a range of 50-65 DEG C; the optimum action pH value is 4.5; over 80% of enzyme activity can be retained in a pH range of 3.0-6.0. The alpha-glucosidase is capable of efficiently converting maltose; the content of isomaltose, the content of panose and the content of isomaltotriose in a conversion product are the highest and respectively reach 44.1%, 23.0% and 21.2%; the total content of the isomaltose, the panose and the isomaltotriose is 88.3%. Therefore, the alpha-glucosidase can be widely applied to production of isomalto oligosaccharides and has a wide market prospect.

The invention belongs to the technical field of enzyme engineering, and particularly relates to a method for preparing isomaltooligosaccharides from alpha-glucosidase through synchronous saccharification and glucoside conversion. The half-life t1 / 2 of the alpha-glucosidase is increased by 3.7 times at 66 DEG C; the proportion of core components (IG2 (Immune Globulin 2)+P+IG3 (Immune Globulin 3)) of the isomaltooligosaccharides, which are prepared by adopting the alpha-glucosidase, high-temperature-resistant alpha-amylase, intermediate-temperature alpha-amylase, beta-amylase and pullulanase to carry out starch liquefaction and through the synchronous saccharification and glucoside conversion, in dry substances reaches 52 to 59 percent.

The invention provides a method of producing isomalto-oligosaccharide by an enzyme method through taking starch and a derivative thereof as raw materials. The isomalto-oligosaccharide is formed by performing combinational expression on an alpha-glucosidase encoding gene from aspergillus niger and performing catalyzed synthesis on immobilized alpha-glucosidase, and quick separation and recycling of a product-immobilized enzyme mixture are realized by a non-aqueous double-phase system. According to the method, the isomalto-oligosaccharide is catalytically produced by adding immobilized alpha-glucosidase microspheres through the double-phase reaction system; after a reaction, the immobilized enzyme can be automatically distributed into an organic phase, thus realizing separation of the immobilized enzyme from a product. The method has the characteristics of mild reaction condition, no need of adding other crosslinking agents, good economy, reduction of the production cost, high enzyme activity recycling rate of the immobilized enzyme, good operation stability and the like. The isomalto-oligosaccharide product obtained by the method can be widely applied to the important fields of medicines, foods, feeds and the like.

The invention relates to application of L-ascorbyl glycoside compounds, such as 2-O-beta-D-glucosyl-L-ascorbic acid, 3-O-beta-D-glucosyl-L-ascorbic acid and 2-O-beta-D-galactosyl-L-ascorbic acid, to preparation of alpha-glucosidase inhibitors. The L-ascorbyl glycoside compounds are remarkable in alpha-glucosidase inhibition activity and have the inhibition effect approximate to that of voglibose, thereby being developed and applied as the alpha-glucosidase inhibitors.

The invention discloses a method for preparing a non-milk probiotic beverage based on an aspergillus oryzae culture, which belongs to the technical field of fermented beverages. The method comprises the following steps: mixing wheat bran and bean pulp, subsequently steaming, cooling down, subsequently inoculating aspergillus oryzae to culture, further pretreating wheat bran so as to obtain a pretreated wheat bran mixture, further regulating the moisture of the aged culture, subsequently mixing with the pretreated wheat bran mixture, stirring, performing hydrolysis and separating the hydrolysate so as to obtain an extract, further sterilizing the extract, subsequently inoculating lactic acid bacteria to ferment so as to obtain fermentation liquor, and preparing the beverage by using the fermentation liquor. The beverage prepared by using the method disclosed by the invention is unique in favor and taste, and has the functions of antioxidation, alpha-glucosidase resistance and the like, the number of viable probiotics can be 5*10<9>cfu / ml, the total phenols content can be 134mg / 100ml, the inhibitory activity of the alpha-glucosidase can be 90%, and great nutrition and healthcare functions are achieved.

The invention provides a method for preparing hulless oat blood sugar reducing polypeptide with hulless oat albumen powder. The method comprises the steps that degreased hulless oat albumen powder is added into pure water at the material to liquid ratio of 1:(100-500), the pH value is regulated to be 1.0-3.0 with 1M hydrochloric acid, pepsin is added according to E / S of 1:(50-100), enzymolysis is carried out for 1-4 h at 37 DEG C, and primary enzymatic hydrolysate is obtained; the pH value of the primary enzymatic hydrolysate is regulated with 1M NaoH to be 7.0-8.0, then trypsin, alpha-chymotrypsin and elastase are added according to E / S of 1:(50-100), enzymolysis is carried out for 1-4 h at 37 DEG C, and secondary enzymatic hydrolysate is obtained; the secondary enzymatic hydrolysate is heated to 95 DEG C for enzyme denaturalixation for 15 min, spraying drying is carried out, and polypeptide containing alpha glucosidase inhibiting activity is obtained. The process is simple, the production cost is low, and the product is safe and free of toxic or side effects. The hulless oat blood sugar reducing polypeptide prepared through the method can be applied in preparing medicine or health-care products for preventing and treating diabetes.

The invention discloses a preparation method of soybean flavored crispy purple rice. The soybean flavored crispy purple rice is mainly composed of following components in parts by weight: 10 to 30 kilograms of purple rice, 1 to 3 kilograms of maltodextrin, 1 to 3 kilograms of boiled soybean powder, and a right amount of spices and edible plant oil. The crispy purple rice is characterized by also comprising following components: alpha-glucosidase with a content of 10000 to 30000 U per kilogram of purple rice, transglutaminase with a content of 100 to 300 U per kilogram of purple rice and notatin preparation with content of 700 to 1100 U per kilogram of purple rice. The soybean flavored crispy purple rice has the advantages of rapid cooking and energy saving, has a strong purple rice flavor and a moderate soybean flavor, tastes crispy, and has no hard core. The raw materials are natural, do not contain chemical compounds such as antiseptic and raising agent, and palm oil is not used, so that the crispy purple rice is not only tasty, but also more nutritional and healthier.

The invention relates to an alpha-glucosidase mutant and application thereof, in particular to an alpha-glucosidase mutant and application of the alpha-glucosidase mutant to production of isomaltooligosaccharide and resistant dextrin, and belongs to the technical field of gene engineering. An alpha-glucosidase mutant gene from aspergillus nidulans is transferred into pichia pastoris to be expressed; the recombinant alpha-glucosidase is used for preparing isomaltooligosaccharide and resistant dextrin; under the conditions of the temperature being 45 DEG C, the pH being 5.5, the enzyme adding quantity being 5 U / g and the substrate concentration being 300 g / L, the yield of the isomaltooligosaccharide reaches 216 g / L; the conversion rate is 72 percent. When pyrodextrin is used as a substrate,three enzymes of branching enzymes, alpha-CGT enzymes and alpha-glucosidase are compounded to improve the ingredients of resistant dextrin; the resistant ingredients of the pyrodextrin can be improvedto 70 percent from the original 44 percent. By the method, the production efficiency is effectively improved; the production cost is reduced.

A nutritional supplement composition contains inhibitors of alpha-glucosidase and alpha-amylase substantially in the absence of lipase inhibitors. The composition can include touchi extract and phaseolamin as the alpha-glucosidase and alpha-amylase inhibitor, respectively. A method of limiting the absorption of carbohydrates contained in a foodstuff includes administering the nutritional supplement composition prior to consumption of the carbohydrates, and a method of promoting weight loss in an individual includes administering the nutritional supplement composition to the individual over a period of days.

The invention discloses clone and expression of an Alpha-glucosidase (abbreviated as AGLU enzyme) gene and belongs to the fields of enzyme gene engineering and enzyme engineering. The invention obtains the aglu gene with SEQ ID NO of 1 through an Aspergillus niger (A.niger) WX-07 total DNA, and aglu cDNA uses an expression vector of plasmid pPIC9K and an expression parasitifer of pichia stipitis (P.pastoris), thus realizing extracellular dissolubility expression of the aglu gene; and the aglu cDNA totally has 2880 nucleic acid and encoding of 960 amino acid, constructs eukaryotic expression plasmid and transforms Pichia pastoris KM71 for AGLU enzyme expression. The recombinant enzyme has transnucleosidation activity, can generate isomalto oligosaccharide through maltose transnucleosidation, is applicable to the requirements of industrial application including food, feeding stuffs and medicaments and the like, and can be applied into the industrial production of isomalto oligosaccharide.

The invention provides a novel Moluo glycoside with natural source as an alpha-glucosidase depressant, and discloses a process for preparing the Moluo glycoside from natural plants such as Patrinia villosa Juss. and cornus officinalis. The invention also discloses the use of Muluo in treating diabetes.

The invention discloses a preparation method of aspergillus-oryzae fermented beverage based on wheat-bran bean pulp and belongs to the technical field of fermented beverage. The preparation method comprises the following steps of mixing and cooking the wheat-bran bean pulp, cooling, then inoculating aspergillus oryzae, culturing to obtain mature culture, then hydrolyzing the mature culture, separating hydrolysate to obtain extracting solution and utilizing the extracting solution to prepare the fermented beverage. The beverage prepared by utilizing the method has the characteristics of being natural, safe, delicious and health, contains rich active ferments such as protease and amylase and phenolic substances and such as oligopeptides with functions of resisting oxidation and inhibiting alpha-glucosidase, wherein the content of total phenols in a product can reach 205mg / 100ml, the inhibition ratio of alpha-glucosidase can reach 92.41% so as to be very beneficial to controlling the blood sugar of a patient with diabetes.

The invention provides an application of a waxberry flesh extract in preparation of an alpha-glucosidase inhibitor. The waxberry flesh extract is prepared through extraction and concentration with methanol, and extraction separation by a solid phase extraction column. Preliminary activity screening tests prove that crude flesh extracts of different waxberry varieties all show alpha-glucosidase inhibition activity. The alpha-glucosidase inhibition activity of different elution components after enrichment of the crude extracts through the solid phase extraction column is improved remarkably, and especially the activity of 50% elution components is much higher than that of positive control acarbose. The application has great significance in reasonable use of waxberry resources and increase of additional values of waxberry products.

The invention discloses a novel strain Monascus purpureus Zhejiang monascus I and its use in preparation of 1-deoxynojirimycin. The Monascus purpureus Zhejiang monascus I is preserved in the China general microbiological culture collection center in No. 3, Courtyard I, Beichen West Road, Chaoyang District, Beijing City on July 18, 2014, and has a preservation number of CGMCC No. 9553. Through fermentation culture of the Monascus purpureus Zhejiang monascus I, 1-deoxynojirimycin culture can be obtained. 1-deoxynojirimycin has alpha-glucosidase inhibition activity. The novel strain Monascus purpureus Zhejiang monascus I and the 1-deoxynojirimycin culture obtained by fermentation of the Monascus purpureus Zhejiang monascus I have wide application prospects.

The invention discloses a pharmaceutical composition for treating diabetes and indications thereof. The effective component of the medical composition for diabetes and indications consists of a medicinal extract, a coenzyme Q and folic acid. The medicinal extract consists of extracts of radix curcumae, rheum officinale, rhizoma curcumae longae, aloe and Polygonum cuspidatum. The medical composition for treating diabetes and indications can greatly inhibit the activities of three target enzymes: alpha-glucosidase, hexokinase and aldose reductase, and can effectively inhibit rise of blood sugar and blood fat in a diabetic animal model as well as treat indications induced by diabetes such as hyperlipidemia and retinopathy.

The invention provides a peanut skin extract for regulating the activity of amylolytic enzyme. The extract is obtained by performing extraction and centrifugation on peanut skins with an extraction solvent, wherein the extraction solvent is water, alcohol, aqueous alcohol or a combination of the three, the regulation refers to inhibition of the activity, the amylolytic enzyme is alpha-amylase or alpha-glucosidase, and the extract is used for inhibiting amylolysis and slowing down the intestinal absorption of carbohydrates.

The invention discloses a preparation method for Chinese hawthorn leaf extract with the alpha-glucosidase restraining effect. The method includes the following steps of preparing Chinese hawthorn leaf powder, preparing a Chinese hawthorn leaf extracting solution through an ultrasonic assistance method, obtaining a concentrated extracting solution, obtaining a water-insoluble object, conducting chromatography on the water-insoluble object through polyacrylamide, obtaining effluent with a methanol solution as eluant, and conducting pressure-reduction concentrating and drying to obtain the target extract. The separation, extraction and preparation method is easy and feasible, and has the industrialized preparation potential; the enzyme activity restraining rate is higher than that of existing acarbose sold on the market; raw materials are wide in source, low in price and easy to obtain, and comprehensive utilization of by-products is achieved.

The invention relates to a method for preparing a bitter gourd solid beverage, in particular to the technical field of food preparations. The method comprises the following steps that pulp and seeds of bitter gourds are separated; the separated pulp of the bitter gourds is prepared into bitter gourd instant powder; the separated seeds of the bitter gourds are prepared into bitter gourd small molecule polypeptide; auxiliary materials are added to the bitter gourd instant powder and the bitter gourd small molecule polypeptide to prepare the bitter gourd solid beverage. According to the method, with the fresh bitter gourds as a raw material, the pulp of the bitter gourds is subjected to homogenization, heat-preservation enzymatic hydrolysis, filtration, concentration and spray drying to prepare the bitter gourd instant powder, and the seeds of the bitter gourds are subjected to degreasing, enzymatic hydrolysis and ultrafiltration classification treatment to prepare the bitter gourd smallmolecule polypeptide; the bitter gourd instant powder, the bitter gourd small molecule polypeptide and the auxiliary materials are mixed to prepare the bitter gourd solid beverage with a blood glucosereducing effect through a wet-method granulation process. The prepared bitter gourd small molecule polypeptide has a blood glucose reducing effect similar to that of acarbose, and has an obvious function of inhibiting alpha-amylase and alpha-glucosidase.

The invention discloses bioactivity polypeptide and an application thereof to preparation of an alpha-glucosidases inhibitor. The amino acid sequence of the bioactivity polypeptide is as shown in SEQID NO.1, the name of the bioactivity polypeptide is AGI-1, the AGI-1 consists of 10 amino acid residues, wherein the 10 amino acid residues contain 2 pieces of cysteine which form a pair of intramolecular disulfide bonds, and through MALDI-TOF mass spectrometric detection, the molecular weight is 1102.3 Da. The physical and chemical properties of purified freeze-dried powder of the polypeptide AGI-1 are white or off-white loose bodies which are odorless and dissolve in water easily, and an aqueous solution is nearly colorless and transparent. The AGI-1 can restrain activity of alpha-glucosidase, and the IC50 value is 1.54 mM.

The invention refers to the field of traditional Chinese medicine, and especially relates to furoate glucoside analogue 1 and purpose thereof of using as alpha-glycosidase inhibitor. The structure of the furoate glucoside analogue 1 is 3-(furan-2-methylcarbonyl)oxyglucopyranose aldehyde acid-1 alpha-4-D-glucopyranose-1 alpha -4-D-glucopyranose. The furoate glucoside analogue 1 has strong inhibition ability to alpha-glucosidase, and can be further developed to be drugs for treating and assisting the treatment of 2 type diabetes.

The invention specially discloses a fermented milk beverage and a preparation method thereof. The preparation method comprises the following steps of performing inoculating with paenibacillus polyxyma( Paenibacillus polymyxa ) CGMCC No.10062 to skim milk, performing fermentation to obtain fermented milk, wherein the blending liquid comprises a sweetening agent and / or a sour agent, uniformly mixing the blending liquid with the prepared fermented milk, performing homogenizing, and performing sterilization so that the fermented milk beverage is obtained. The preparation method disclosed by the technical scheme comprises the following steps of firstly, adopting the paenibacillus polyxyma ( Paenibacillus polymyxa ) CGMCC No.10062, performing fermentation with skim milk as a culture medium, performing blending, performing homogenizing and performing sterilization so as to prepare the fermented milk beverage having alpha-glucosidases restrain activity. The paenibacillus polyxyma CGMCC No.10062 is disclosed to have the new purpose of generating the fermented milk beverage having alpha-glucosidases restrain activity, from the fermentation skim milk.

The invention discloses a preparation method of an Inonotus obliquus extract which can be used as an Alpha-glucosaccharase inhibitor. In the preparation method, ethanol is firstly adopted to heat and reflow the Inonotus obliquus so as to obtain an ethanol extract, then the ethanol extract is fully dispersed into distilled water and is extracted by sherwood oil so as to remove low-polarity components in the extract, which have no inhibition activity to the Alpha-glucosaccharase; and then the raffinate is extracted by ethyl acetate, thereby obtaining the extract which has strong inhibition activity to the Alpha-glucosaccharase; and the yield rate is 2 to 4 percent. Experiment proves that the Inonotus obliquus extract obtained by the preparation method provided by the invention has very strong inhibition activity to the Alpha-glucosaccharase; when the dosage is 400 [mu]g, the inhibition rate can reach 80.5 percent, with the IC50 of 103.9 [mu]g / ml; therefore, the method can be used for developing sugar-reducing medicament. Moreover, the preparation method also has the advantages of simple technique, convenient operation, and the like.

The invention provides a method for preparing polyphenol polymers with alpha-glucosidase inhibiting activity from teng tea. The method for preparing polyphenol polymers with alpha-glucosidase inhibiting activity from teng tea is characterized by comprising the following steps of (1) teng tea water extract preparation: putting the teng tea into boiling water, soaking the teng tea for a period of time, filtering liquid by gauze, and performing freeze drying on filter liquid to obtain a teng tea water extract; (2) column chromatography separation: dissolving the teng tea extract by a 50-percent methanol water solution, using an upper chromatography column, firstly flushing away impurities by the methanol water solution with a certain concentration, then performing elution by an acetone water solution with a certain concentration, and collecting an elution solution; and (3) membrane dialysis: performing vacuum rotary evaporation on the obtained elution solution to remove acetone, putting the obtained water solution into a dialysis bag, performing dialysis by water for a certain time, collecting a sample solution in the bag, performing freeze drying and obtaining the polyphenol polymers. The method has the advantages that the production process is simple, and the cost is low.

The invention relates to polyoxygenated cyclohexene derivatives separated from a fermentation product of Phomopsis sp. YE3250, and an application thereof, and belongs to the technical field of microorganisms. Seven polyoxygenated cyclohexene derivatives are obtained through conventional fermentation, extraction and separation of the strain YE3250, and are respectively named as polyoxygenated cyclohexene A, polyoxygenated cyclohexene B, polyoxygenated cyclohexene C, polyoxygenated cyclohexene D, polyoxygenated cyclohexene E, polyoxygenated cyclohexene F and polyoxygenated cyclohexene G. The polyoxygenated cyclohexene derivatives have an obvious inhibitory activity on alpha-glucosidase, and have a potential use in the preparation of new drugs for preventing and treating diabetes.