Application of radix physochlainae extract in preparation of drugs for preventing and treating myopia
An extract and myopia technology, applied in the field of biomedicine, can solve problems such as the improvement effect of myopia that has not yet been seen, and achieve the effect of delaying the progress of myopia, high safety, and remarkable therapeutic effect
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Embodiment 1
[0039] Example 1 Preparation of Huashan Ginseng Extract by Ultrasonic Extraction
[0040] The preparation method of Huashan ginseng extract comprises the following steps:
[0041] (1) extraction
[0042] Grind Huashan ginseng to make Huashan ginseng powder, weigh 2g of Huashan ginseng powder and put it into a conical flask, add 70% ethanol as an extractant according to the ratio of solid to liquid 1:25 (g / mL), at a temperature of 30°C, Ultrasonic (350W, 50Hz) treatment was performed for 30 minutes; vacuum filtration while hot, and the filtrates were combined to obtain an extract.
[0043] (2) concentrated
[0044] Concentrate the extract under reduced pressure until it has no alcohol smell, and set it aside.
Embodiment 2
[0045] Example 2 Preparation of Huashan ginseng extract by heating and reflux extraction
[0046] The preparation method of Huashan ginseng extract comprises the following steps:
[0047] (1) extraction
[0048] Grind Huashan ginseng to make Huashan ginseng powder, weigh 200g Huashan ginseng powder, put it into a 2L round bottom flask, add 70% ethanol solution until the sample can be completely soaked, and extract in a constant temperature (100°C) water bath for 3 hours , the obtained extract is filtered with gauze, and the filtrate is collected; the above operation is repeated once again; finally, the filtrate is combined to obtain the extract.
[0049] (2) concentrated
[0050] Concentrate the extract under reduced pressure until it has no alcohol smell, and set it aside.
Embodiment 3
[0051] Example 3 Preparation of Huashan Ginseng Extract by Decoction
[0052] The preparation method of Huashan ginseng extract comprises the following steps:
[0053] (1) extraction
[0054] Grind Huashan ginseng to make Huashan ginseng powder, weigh 200g Huashan ginseng, add water to extract twice, each time for 2 hours, collect the filtrate, concentrate under reduced pressure to a clear paste with a relative density of 1.25-1.35 (60°C), add ethanol For precipitation, adjust the amount of ethanol to 70%, let stand, and take the supernatant.
[0055] (2) concentrated
[0056] Concentrate the supernatant under reduced pressure until it has no alcohol smell, and set it aside.
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